Regulation and localization of sperm akaps during capacitation, activation, induced acrosomal reaction and binding to the oocyte
Kyselová, Vendula; Pěknicová, Jana
2003 - English
Recent evidence suggests that the cAMP-dependent protein kinase A (PKA) is localized to specific subcellulatr compatrments trought association with A Kinase Anchoring Proteins (AKAPs). The AKAPs now represent a functionally related family of regulatory proteins containing a conserved PKA binding domain and a unique targeting sequence that direct the PKA-AKAP complex to subcellular structures.
Keywords:
akap; acrosome
Available at various institutes of the ASCR
Regulation and localization of sperm akaps during capacitation, activation, induced acrosomal reaction and binding to the oocyte
Recent evidence suggests that the cAMP-dependent protein kinase A (PKA) is localized to specific subcellulatr compatrments trought association with A Kinase Anchoring Proteins (AKAPs). The AKAPs now ...
Properties of serine proteinase inhibitors from boar seminal plasma
Jelínková, Petra; Maňásková, Pavla; Tichá, M.; Jonáková, Věra
2002 - English
Inhibitors of proteinases are important components of seminal plasma. Their biological function in seminal plasma is to protect protein components of seminal plasma and male and female reproductive tracts against proteolytic degradation. My work is concentrated on characterization of inihibitors found in seminal plasma.
Keywords:
inhibitors; seminal plasma; boar serine proteinase inhibitors; inhibitory
Available at various institutes of the ASCR
Properties of serine proteinase inhibitors from boar seminal plasma
Inhibitors of proteinases are important components of seminal plasma. Their biological function in seminal plasma is to protect protein components of seminal plasma and male and female reproductive ...
Study of aggregated forms of proteinase inhibitors from boar seminl plasma by gel chromatography
Jelínková, Petra; Maňásková, Pavla; Tichá, M.; Jonáková, Věra
2002 - English
Most of proteins in seminal plasma exist under physiological conditions in the form of aggregates. The study of the presence of proteinase inhibitosr in different associated forms of boar seminal plasma proteins is the subject of our present communication.
Keywords:
Gel chromatography; proteinase inhibitors; seminal plasma
Available at various institutes of the ASCR
Study of aggregated forms of proteinase inhibitors from boar seminl plasma by gel chromatography
Most of proteins in seminal plasma exist under physiological conditions in the form of aggregates. The study of the presence of proteinase inhibitosr in different associated forms of boar seminal ...
Serine proteinase inhibitors isolated from boar seminal plasma
Jelínková, Petra; Maňásková, Pavla; Tichá, M.; Jonáková, Věra
2002 - English
Serine proteinase inhibitors were found in boar seminal plasma. The work was concentrated on isolation and characterization of proteinase inhibitors and study of the existence of inhibitors in complexes with other proteins in seminal plasma.
Keywords:
proteinase inhibitors; seminal plasma; proteins; bílkoviny
Available at various institutes of the ASCR
Serine proteinase inhibitors isolated from boar seminal plasma
Serine proteinase inhibitors were found in boar seminal plasma. The work was concentrated on isolation and characterization of proteinase inhibitors and study of the existence of inhibitors in ...
Protein interactions involved in the formation of aggregated forms of boar seminal plasma proteins
Maňásková, Pavla; Balínová, P.; Tichá, M.; Jonáková, Věra
2002 - English
Mutual interactions of isolated proteins were tested by means of gel chromatography. The results obtained confirmed an existence of interactions between spermadhesins of the AQN and AWN families as well as between proteins involved in the formation of heterodimer PSPI/PSPII.
Keywords:
spermadhesins; aggregates; aggregated forms
Available at various institutes of the ASCR
Protein interactions involved in the formation of aggregated forms of boar seminal plasma proteins
Mutual interactions of isolated proteins were tested by means of gel chromatography. The results obtained confirmed an existence of interactions between spermadhesins of the AQN and AWN families as ...
Effect of endocrine disruptors on the microtubules of MCF-7 cells
Pěknicová, Jana; Kubátová, Alena; Kyselová, Vendula; Buckiová, Daniela; Paleček, J.; Dráber, Pavel
2002 - English
Keywords:
microtubules; endocrine disruptor; MCF-7 cell
Available at various institutes of the ASCR
Effect of endocrine disruptors on the microtubules of MCF-7 cells
Cryocrystallography of biological Macromolecules
Řezáčová, Pavlína
2002 - English
Crystals of biological macromolecules at near to room temperature are sensitive to X-rays and frequently suffer from radiation damage, especially when X-ray experiments are carried out on highly intense synchrotron beamlines. Performing such experiments at cryogenic temperatures greatly reduce, or eliminate radiation damage and thus produce higher quality diffraction data.
Keywords:
cryocrystallography; radiation demage; protein crystals
Available at various institutes of the ASCR
Cryocrystallography of biological Macromolecules
Crystals of biological macromolecules at near to room temperature are sensitive to X-rays and frequently suffer from radiation damage, especially when X-ray experiments are carried out on highly ...
Proteins from boar prostate secretion: their isolation and characterization
Maňásková, Pavla; Ryšlavá, H.; Tichá, M.; Jonáková, Věra
2002 - English
Prostate proteins were separated by affinity chromatography on immobilized heparin. Major identified components of prostate secretion:beta-microseminoprotein, serotransferin, serum albumin, PSP I and II spermadhesins.
Keywords:
prostate; accessory sexual glands; boar
Available at various institutes of the ASCR
Proteins from boar prostate secretion: their isolation and characterization
Prostate proteins were separated by affinity chromatography on immobilized heparin. Major identified components of prostate secretion:beta-microseminoprotein, serotransferin, serum albumin, PSP I and ...
Gamma-tubulin and its function
Dráber, Pavel
2002 - English
Keywords:
gamma-tubulin; microtubules; nucleation
Available at various institutes of the ASCR
Gamma-tubulin and its function
An approach for isolation of human seminal plasma proteins
Kraus, Marek; Tichá, M.; Jonáková, Věra
2002 - English
Human seminal plasma contains various type of protein. For their isolation were used these methods: affinity chromatography, gel chromatography, HPLC etc. The cross-reactivity of antibodies against boar seprmadhesing AQN and AWN was studied with human seminal proteins.
Keywords:
human seminal plasma; gel chromatography; 2.D-electrophoresis
Available at various institutes of the ASCR
An approach for isolation of human seminal plasma proteins
Human seminal plasma contains various type of protein. For their isolation were used these methods: affinity chromatography, gel chromatography, HPLC etc. The cross-reactivity of antibodies against ...
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