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Analysis of individual organelles
Václavek, Tomáš; Manion-Fisher, D.; Muratore, K.; Taylor, T.; Křenková, Jana; Foret, František; Arriaga, E. A.
2014 - anglický
This poster describes a method of organelle analysis using capillary electrophoresis instrumentation with high sensitivity laser induced fluorescence detection.
Klíčová slova:
organelle; capillary; fluorescence
Dokument je dostupný na externích webových stránkách.
Analysis of individual organelles
This poster describes a method of organelle analysis using capillary electrophoresis instrumentation with high sensitivity laser induced fluorescence detection.
Identification of nosocomial infection by electrophoretic techniques
Kubesová, Anna; Moravcová, Dana; Tesařová, Marie; Horká, Marie
2014 - anglický
The detection and identification of pathogens currently relies upon a very diverse range of techniques and skills, from traditional culturing and taxonomic procedures to modern molecular biology based methods. However, conventional laboratory methods are time consuming, laborious, and they may provide both false positive or negative results, especially for closely related microorganisms. In this study, we suggest capillary electrophoresis techniques for differentiation and characterization of Methicillin-resistant and Methicillin-susceptible Staphylococcus aureus.
Klíčová slova:
capillary electrophoresis; nosocomial infection; Staphylococcus aureus
Dokument je dostupný na externích webových stránkách.
Identification of nosocomial infection by electrophoretic techniques
The detection and identification of pathogens currently relies upon a very diverse range of techniques and skills, from traditional culturing and taxonomic procedures to modern molecular biology based ...
Computer assisted development of a pneumatic electrospray nebulizer
Járvás, G.; Szigeti, M.; Grym, Jakub; Foret, František; Guttman, A.
2014 - anglický
In this study we demonstrate how computer simulations can assist the development of a novel microfabricated pneumatic electrospray nebulizer. The microdevice under consideration is designed for electrospray mass spectrometry interfacing without the need to fabricate an electrospray needle. This new interfacing strategy requires comprehensive evaluation of the differently designed chip layouts. The flow evaluation was supported by computational fluid dynamics simulations. It was proved with experimental validation of the simulation results that the developed models were practical tools for the numerical investigation of the separations-ESI-MS coupling.
Klíčová slova:
microfabricated ESI-MS; electrospray mass spectrometry; pneumatic electrospray nebulizer
Dokument je dostupný na externích webových stránkách.
Computer assisted development of a pneumatic electrospray nebulizer
In this study we demonstrate how computer simulations can assist the development of a novel microfabricated pneumatic electrospray nebulizer. The microdevice under consideration is designed for ...
Isotachophoretic determination of ketone bodies and lactate in cattle serum
Vyskočilová, V.; Pechová, A.; Pantůčková, Pavla
2014 - anglický
The aim of the work was the optimization of isotachophoretic determination of lactate and ketone bodies (beta-hydroxybutyrate and acetoacetate) for veterinary medicine. The measurement of these metabolites is very important for the evaluation of metabolic diseases of cattle. The complete separation of these three analytes together with other possible blood components was achieved using 10 mmol/l hydrochloric acid with beta-alanine as a leading electrolyte at pH = 3.15 and 10 mmol/l sodium propionate as a terminating electrolyte. Limits of detection determined from the standard mixture for acetoacetate, lactate and beta-hydroxybutyrate were 3.75 mu mol/l, 3.96 mu mol/l and 7.46 mu mol/l, respectively.
Klíčová slova:
cattle serum; lactate; ketone bodies; isotachophoresis
Dokument je dostupný na externích webových stránkách.
Isotachophoretic determination of ketone bodies and lactate in cattle serum
The aim of the work was the optimization of isotachophoretic determination of lactate and ketone bodies (beta-hydroxybutyrate and acetoacetate) for veterinary medicine. The measurement of these ...
Microbicidal properties and chemical composition of essential oils
Křůmal, Kamil; Večeřa, Zbyněk
2014 - anglický
The microbicidal properties of 6 essential oils (EOs; Lavandula angustifolia, Cymbopogon nardus, Citrus aurantifolia, Juniperus communis, Myrtus communis and Cinnamomum zeylanicum ) for 17 microorganisms were determined using the vapour-agar contact method. The most effective EO (i.e. Lavandula angustifolia ) whose volatile components provided the sufficient microbicidal properties was chosen for detailed study of chemical composition.
Klíčová slova:
essential oil; microbicidal properties; denuder
Dokument je dostupný na externích webových stránkách.
Microbicidal properties and chemical composition of essential oils
The microbicidal properties of 6 essential oils (EOs; Lavandula angustifolia, Cymbopogon nardus, Citrus aurantifolia, Juniperus communis, Myrtus communis and Cinnamomum zeylanicum ) for 17 ...
Direct coupling of supported liquid membrane extractions to capillary electrophoresis. Sensitivity enhancement in analyses of untreated complex samples
Pantůčková, Pavla; Kubáň, Pavel; Boček, Petr
2014 - anglický
Extractions across supported liquid membrane (SLM) were in-line coupled to capillary electrophoresis (CE) commercial system for direct injection of basic drugs from raw complex samples. A micro-extraction unit was inserted into the CE system sampling device and ensured excellent sample clean-up and injections directly from the membrane surface. Transient isotachophoresis (t-ITP) combined with large electrokinetic injections of selectively extracted basic analytes increased the sensitivity of the analytical method up to 300-fold when compared with conventional hydrodynamic injections and capillary zone electrophoresis (CZE) of the basic analytes.
Klíčová slova:
capillary electrophoresis; supported liquid membrane; transient isotachophoresis
Dokument je dostupný na externích webových stránkách.
Direct coupling of supported liquid membrane extractions to capillary electrophoresis. Sensitivity enhancement in analyses of untreated complex samples
Extractions across supported liquid membrane (SLM) were in-line coupled to capillary electrophoresis (CE) commercial system for direct injection of basic drugs from raw complex samples. A ...
Influence of inhaled manganese oxides nanoparticles on mass of internal organs in mice
Zeman, T.; Dočekal, Bohumil; Mikuška, Pavel; Buchtová, Marcela; Míšek, Ivan; Šerý, Omar; Večeřa, Zbyněk
2014 - anglický
Results of the statistical analysis of a long-term inhalation experiment on laboratory mice are presented. During 17 weeks of the experiment, the experimental group was exposed to inhaled manganese oxides nanoparticles. Manganese oxides (MnO.Mn2O3) nanoparticles (MnONPs) were synthesized continuously via aerosol route in a hot wall tube flow reactor using a thermal decomposition of metal organic precursor manganese(II)acetylacetonate in the flow tube reactor (in vertical position) at temperature 750 °C in the presence of 30 vol% of oxygen. The concentration of produced MnONPs at the reactor output was in the range 1-3 × 107 particles/cm3 and the size of generated nanoparticles MnO.Mn2O3 was in the range 7-50 nm. Before entering the inhalation chamber, MnONPs in the mixture of N2, O2 and air (at total flow rate of 3 L/min) were further diluted using filtrated humidified air (20 L/min) at temperature 21 °C resulting in the MnONPs concentration 2 × 106 particles/cm3. At regular time intervals the mass of selected internal organs of mice from both experimental and control group was assessed. It has been proven that inhaled nanoparticles are able to influence the mass of internal organs of mice. Statistically significantly lower mass of kidneys, liver and spleen and higher mass of pancreas have been found in the experimental group compared to the control group.
Klíčová slova:
inhaled nanoparticles; manganese oxides nanoparticles; changes in internal organ mass of mice; long-term inhalation experiment
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Influence of inhaled manganese oxides nanoparticles on mass of internal organs in mice
Results of the statistical analysis of a long-term inhalation experiment on laboratory mice are presented. During 17 weeks of the experiment, the experimental group was exposed to inhaled manganese ...
Monosaccharide anhydrides in atmospheric aerosols - optimization of separation and detection by means of LC-MS technique
Čmelík, Richard; Coufalík, Pavel; Mikuška, Pavel
2014 - anglický
We report a simple analytical method, based on high-performance liquid chromatography with mass spectrometric detection, which was developed and utilized to determine monosaccharide anhydrides in atmospheric aerosols.
Klíčová slova:
monosaccharide anhydrides; LC-MS; aerosols
Dokument je dostupný na externích webových stránkách.
Monosaccharide anhydrides in atmospheric aerosols - optimization of separation and detection by means of LC-MS technique
We report a simple analytical method, based on high-performance liquid chromatography with mass spectrometric detection, which was developed and utilized to determine monosaccharide anhydrides in ...
Quantum dot-based Fӧrster resonance energy transfer bioanalysis
Datinská, Vladimíra; Klepárník, Karel; Belšánová, B.; Minárik, M.; Foret, František
2014 - anglický
We describe a new configuration for Fӧrster resonance energy transfer (FRET) between a quantum dot (QD) donor and a fluorescence dye acceptor attached to DNA molecule. This FRET sensor is aimed to be used in genomic analysis for DNA mutation detection applicable especially in cancer research.
Klíčová slova:
FRET; quantum dot; DNA
Dokument je dostupný na externích webových stránkách.
Quantum dot-based Fӧrster resonance energy transfer bioanalysis
We describe a new configuration for Fӧrster resonance energy transfer (FRET) between a quantum dot (QD) donor and a fluorescence dye acceptor attached to DNA molecule. This FRET sensor is aimed to be ...
Photo-induced flow-injection determination of nitrate in water
Mikuška, Pavel; Čapka, Lukáš; Večeřa, Zbyněk; Kalinichenko, I.; Kellner, J.
2014 - anglický
A sensitive flow-injection method for the chemiluminescent determination of ultra-low concentration of nitrate in water.
Klíčová slova:
flow injection analysis; nitrate; chemiluminescence
Dokument je dostupný na externích webových stránkách.
Photo-induced flow-injection determination of nitrate in water
A sensitive flow-injection method for the chemiluminescent determination of ultra-low concentration of nitrate in water.
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