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Analysis of antimicrobial peptides by capillary electrophoresis
Ehala, Sille; Niederhafner, Petr; Čeřovský, Václav; Řezanka, P.; Sýkora, D.; Král, V.; Kašička, Václav
2011 - English
Capillary electrophoresis was used for the purity degree determination of new antimicrobial peptides: octadecapeptide melectin and tetradecapeptide mastoparan PDD-B, and their synthetic analogues. Besides, four semiempirical models correlating electrophoretic mobilities of these peptides with their charge and size (relative molecular mass) were applied to predict their probable structures in solution. Additionally, capillaries coated with gold nanoparticles were employed for the separation of mixtures of antimicrobial peptides.
Keywords:
capillary electrophoresis; antimicrobial peptides; gold nanoparticles
Available at various institutes of the ASCR
Analysis of antimicrobial peptides by capillary electrophoresis
Capillary electrophoresis was used for the purity degree determination of new antimicrobial peptides: octadecapeptide melectin and tetradecapeptide mastoparan PDD-B, and their synthetic analogues. ...
Lucifensin, a peptide behind the maggot therapy
Čeřovský, Václav
2011 - English
The larvae of the green bottle fly (Lucilia sericata) are routinely used as a medication for the treatment of necrotic, non-healing and highly infected wound in many hospitals all over the world. We have identified and determined primary structure of the defensin originated from the immune system of Lucilia sericata larvae and named it lucifensin. This is 40 amino acid residues and three intramolecular disulfide bridges peptide. The synthetic lucifensin was active against Gram-positive bacteria and was not hemolytic. We studied the importance of lucifensin disulfide bridges and the N-terminal part of its molecule for the structure and for antimicrobial activity.
Keywords:
lucifensin; maggot therapy; antimicrobial activity; peptide synthesis; disulfide bridge
Available at various institutes of the ASCR
Lucifensin, a peptide behind the maggot therapy
The larvae of the green bottle fly (Lucilia sericata) are routinely used as a medication for the treatment of necrotic, non-healing and highly infected wound in many hospitals all over the world. We ...
Antimicrobial peptides isolated from the venom of wild bee Panurgus calcaratus
Čujová, Sabína; Monincová, Lenka; Slaninová, Jiřina; Bednárová, Lucie; Čeřovský, Václav
2011 - English
Three novel peptides designated as PNG-1, PNG-K and PNG-R were isolated from the venom of the solitary bee Panurgus calcaratus. They exhibited antimicrobial activity against Grampositive and -negative bacteria and fungi. In this work we focused on the characterization of PNG-R which is unique 25 amino acid residues and two disulfide bridges containing peptide. The secondary structure of PNG-R was studied by circular dichroism spectroscopy measured in water and in the presence of trifluoroethanol or sodium dodecyl sulfate.
Keywords:
Panurgus calcaratus; alpha-Helical structures; antimicrobial peptides; disulfide bridges; bee venom
Available at various institutes of the ASCR
Antimicrobial peptides isolated from the venom of wild bee Panurgus calcaratus
Three novel peptides designated as PNG-1, PNG-K and PNG-R were isolated from the venom of the solitary bee Panurgus calcaratus. They exhibited antimicrobial activity against Grampositive and ...
Isolation and study of the properties of recombinant pseudoosmotin
Richterová, K.; Nováková, Martina; Antošová, Z.; Neubauerová, Tereza; Macková, Martina
2011 - English
Antimicrobial peptides represent new clinical compounds with antimicrobial action and they could participate in solving the problem of ever-increasing resistance of microorganisms to the traditional antibiotics. The aim of this work was to study optimal conditions for expression, isolation and purification of recombinant pseudoosmotin from Escherichia coli BL21 (DE3). Pseudoosmotin was isolated from membrane fraction of bacteria and was purified by the affinity chromatography using Ni-NTA. Isolated pseudoosmotin did not show antimicrobial activity against studied microorganisms.
Keywords:
osmotin; antifungal activity; antimicrobial peptides; recombinant proteins
Available at various institutes of the ASCR
Isolation and study of the properties of recombinant pseudoosmotin
Antimicrobial peptides represent new clinical compounds with antimicrobial action and they could participate in solving the problem of ever-increasing resistance of microorganisms to the traditional ...
Activity of antimicrobial peptides from the venom of hymenoptera against Candida albicans biofilms
Putnová, Helena; Fučík, Vladimír; Monincová, Lenka; Čeřovský, Václav; Slaninová, Jiřina
2011 - English
Biofilms are nowadays a serious problem especially with immunosuppressed patients. These highly resistant agglomerations are formed by cells which are firmly adhered to the surface. In this work, the effect of antimicrobial peptides isolated from venom reservoirs of wild bees were tested against Candida albicans biofilms. The results show that some synthetic analogues exhibit potency to kill quickly the yeast biofilm cells. On the contrary, some analogues active against planktonic cells are only little active or inactive against biofilms.
Keywords:
biofilm; antifungal activity; antimicrobial peptides; XTT assay
Available at various institutes of the ASCR
Activity of antimicrobial peptides from the venom of hymenoptera against Candida albicans biofilms
Biofilms are nowadays a serious problem especially with immunosuppressed patients. These highly resistant agglomerations are formed by cells which are firmly adhered to the surface. In this work, ...
Study of antimicrobial peptide induction in Brassica napus
Shevchenko, I.; Neubauerová, Tereza; Macková, Martina; Macek, Tomáš
2011 - English
Development of cheap, effective and environmentally friendly protection of industrial plants is a key problem of mankind, where using of antimicrobial peptides could be a reasonable solution. As a source for isolation of these peptides canola (Brassica napus) was chosen, which was treated by functional analog of salicylic acid – benzothiadiazole (BTH) – to stimulate the immune system. To isolate the responsible antimicrobial peptides, crude buffer extract was obtained. Next steps were precipitation, centrifugation and molecular weight filtration. The further isolation procedure comprised hexane extraction, SPE-C18 (Solid Phase Extraction) to separate hydrophobic and hydrophilic fractions and reversed-phase chromatography. Antimicrobial activity was tested by diffusion method. Fractions were tested on gram-positive and gram-negative bacteria and on yeast Candida scotii.
Keywords:
Brassica napus; induced resistance; antimicrobial peptides
Available at various institutes of the ASCR
Study of antimicrobial peptide induction in Brassica napus
Development of cheap, effective and environmentally friendly protection of industrial plants is a key problem of mankind, where using of antimicrobial peptides could be a reasonable solution. As a ...
Interaction of lasioglossin III with CCRF-CEM cells
Slaninová, Jiřina; Mlsová, V.; Günterová, Jana; Borovičková, Lenka; Čeřovský, Václav
2011 - English
Antimicrobial peptides isolated recently from the venom reservoirs of wild bees are active not only against bacteria and fungi, but also selectively lyze some cancer cell lines. At the same time they have very low hemolytic activity and concentrations required to lyze control normal cell lines are much higher. Here we describe the time course of the effect of lasioglossin III (LL-III) on CCRF-CEM cells alone and in the mixture with human red blood cells using flow cytometry.
Keywords:
lasioglossin; antimicrobial peptides; cancer cells; flow cytometry
Available at various institutes of the ASCR
Interaction of lasioglossin III with CCRF-CEM cells
Antimicrobial peptides isolated recently from the venom reservoirs of wild bees are active not only against bacteria and fungi, but also selectively lyze some cancer cell lines. At the same time ...
Expression of osmotin, an antifungal protein from Nicotiana tabacum in Escherichia coli
Viktorová, J.; Macková, M.; Macek, Tomáš
2011 - English
Plants have evolved a huge variety of proteins involved in the defense against pathogens and adaptation to stressful environments. Plant proteins whose expression is strongly induced in response to infection by pathogens belong to the group of pathogenesis-related (PR) proteins. The family of PR-5 proteins constitutes a group of cysteine-rich proteins including thaumatin, zeamatin and also osmotin. Osmotin is a cationic protein of 205 residues and molecular weight of 24 kDa. It was discovered and characterized in cells of Nicotiana tabacum var. Wisconsin 38. The plasmid harbouring cDNA of osmotin from Nicotiana tabacum was constructed for transformation of Escherichia coli. The osmotin gene was prepared in fusion with histidine tail to facilitate the isolation and purification from bacterial cells. Selection of transgenic colonies was based on antibiotic resistance. The hexahistidine-tagged osmotin was overexpressed in heterologous system by using pET expression vector and purified using immobilized metal affinity chromatography. The expression of osmotin was detected and antifungal activity was tested.
Keywords:
pathogenesis-related proteins; osmotin; antifungal activity; recombinant protein
Available at various institutes of the ASCR
Expression of osmotin, an antifungal protein from Nicotiana tabacum in Escherichia coli
Plants have evolved a huge variety of proteins involved in the defense against pathogens and adaptation to stressful environments. Plant proteins whose expression is strongly induced in response to ...
Synthesis and biological properties of the 2’-trifluoromethyl analogues of tenofovir
Jansa, Petr; Kolman, Viktor; Janeba, Zlatko
2011 - English
A series of acyclic nucleoside phosphonates substituted at the aliphatic part of the molecule by the trifluoromethyl group was prepared but none of the compounds exhibits any anti-HIV or anti-HCV activities.
Keywords:
ANPs; acyclic nucleoside phosphonates; trifluoromethyl group
Available at various institutes of the ASCR
Synthesis and biological properties of the 2’-trifluoromethyl analogues of tenofovir
A series of acyclic nucleoside phosphonates substituted at the aliphatic part of the molecule by the trifluoromethyl group was prepared but none of the compounds exhibits any anti-HIV or anti-HCV ...
Acyclic Nucleoside Phosphonates as Inhibitors of Hypoxanthine-Guanine-Xanthine Phosphoribosyltransferase: New Anti-Malarial Chemotherapy Leads
Hocková, Dana; Holý, Antonín; Česnek, Michal; Baszczyňski, Ondřej; Tichý, Tomáš; Krečmerová, Marcela; Janeba, Zlatko; Skinner-Adams, T. S.; Naesens, L.; Keough, D. T.; de Jersey, J.; Guddat, L. W.
2011 - English
Hypoxanthine-guanine-xanthine phosphoribosyltransferase (HGXPRTase) is a widely recognized target for the discovery of new anti-malarial drugs. Specific acyclic nucleoside phosphonates (ANPs) inhibit HGXPRTase and possess anti-plasmodial activity. Within the framework of a SAR-study, the classical ANPs (e.g. PME-, PMP- and HPMP-derivatives) as well as novel series of compounds were tested to investigate their efficiency and selectivity on the inhibition of P. falciparum, P. vivax and human enzyme.
Keywords:
ANPs; acyclic nucleoside phosphonates; anti-malarial; Plasmodium; HGXPRTase
Available at various institutes of the ASCR
Acyclic Nucleoside Phosphonates as Inhibitors of Hypoxanthine-Guanine-Xanthine Phosphoribosyltransferase: New Anti-Malarial Chemotherapy Leads
Hypoxanthine-guanine-xanthine phosphoribosyltransferase (HGXPRTase) is a widely recognized target for the discovery of new anti-malarial drugs. Specific acyclic nucleoside phosphonates (ANPs) inhibit ...
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