Yes (1442)
No (5)
Number of found documents:
1447
Published from
to
Mannan-BAM, TLR ligands, and anti-CD40 immunotherapy in established murine pancreatic adenocarcinoma: understanding therapeutic potentials and limitations
VENHAUEROVÁ, Anna
2023 - English
The aim of this RNDr. Thesis is focused on understanding therapeutic potentials and limitations of the antitumor MBTA immunotherapy which is based on synergy of TLR agonists, anti-CD40, and phagocytosis stimulating ligands anchored into the tumor cell membranes. In this study, immunotherapy was tested in murine pancreatic adenocarcinoma Panc02 model. Firstly, the short-term and long-term efficacy of MBTA therapy was tested using established subcutaneous Panc02 tumors two times larger than in previous study. Secondly, the work is devoted to better understanding of the adaptive immunity involvement focusing on CD4+ and CD8+ T lymphocytes during the therapy and their effect on tumor volume reduction, long-term survival and resistance against tumor rechallenge. Subsequently, the ability of immunological memory to cross over the blood-brain barrier confirming its potential applicability in metastatic brain tumors was examined. Moreover, the antigen specificity of the immunological memory was evaluated. Finally, the potential of MBTA therapy to cure metastatic disease, represented by bilateral Panc02 mouse model, was studied. In this case, the MBTA therapy manifested a lower therapeutic response. Therefore, it was combined with diverse therapeutic approaches, such as intratumoral application of anti-CTLA-4 antibody, heat-killed Listeria monocytogenes, chemoablation using EtOH, targeting the tumor microenvironment by hyaluronidase, simultaneous injections of MBTA therapy in primary and secondary distant tumors, and its combination with RT. Despite all these combinations, our results showed that only simultaneous application of MBTA therapy into both tumors has potential for the treatment of the bilateral Panc02. The aim of this RNDr. Thesis is focused on understanding therapeutic potentials and limitations of the antitumor MBTA immunotherapy which is based on synergy of TLR agonists, anti-CD40, and phagocytosis stimulating ligands anchored into the tumor cell membranes. In this study, immunotherapy was tested in murine pancreatic adenocarcinoma Panc02 model. Firstly, the short-term and long-term efficacy of MBTA therapy was tested using established subcutaneous Panc02 tumors two times larger than in previous study. Secondly, the work is devoted to better understanding of the adaptive immunity involvement focusing on CD4+ and CD8+ T lymphocytes during the therapy and their effect on tumor volume reduction, long-term survival and resistance against tumor rechallenge. Subsequently, the ability of immunological memory to cross over the blood-brain barrier confirming its potential applicability in metastatic brain tumors was examined. Moreover, the antigen specificity of the immunological memory was evaluated. Finally, the potential of MBTA therapy to cure metastatic disease, represented by bilateral Panc02 mouse model, was studied. In this case, the MBTA therapy manifested a lower therapeutic response. Therefore, it was combined with diverse therapeutic approaches, such as intratumoral application of anti-CTLA-4 antibody, heat-killed Listeria monocytogenes, chemoablation using EtOH, targeting the tumor microenvironment by hyaluronidase, simultaneous injections of MBTA therapy in primary and secondary distant tumors, and its combination with RT. Despite all these combinations, our results showed that only simultaneous application of MBTA therapy into both tumors has potential for the treatment of the bilateral Panc02.
Keywords:
cancer immunotherapy; immunology; cancer; pancreatic adenocarcinoma; TLR agonists; anti-CD40; phagocytosis
Available in the Digital Repository of University of South Bohemia.
Mannan-BAM, TLR ligands, and anti-CD40 immunotherapy in established murine pancreatic adenocarcinoma: understanding therapeutic potentials and limitations
The aim of this RNDr. Thesis is focused on understanding therapeutic potentials and limitations of the antitumor MBTA immunotherapy which is based on synergy of TLR agonists, anti-CD40, and ...
Determinants of Piper (Piperaceae) climber composition in a lowland tropical rainforest in New Guinea
LISNER, Aleš
2023 - English
The study examines the distribution of root climbers from the genus Piper on host trees depending on environmental properties and host tree characteristics in a lowland primary tropical rainforest in Papua New Guinea. More specifically, the study examines the relationship between the climbers' presence and various factors including topography (e.g. slope, altitude), vegetation properties, and host tree characteristics. The findings reveal that Piper climbers tend to avoid higher altitudes, steeper slopes, closed canopy layers, and areas with larger total basal area of host vegetation. The preferences of Piper climbers for certain tree species are primarily influenced by the properties of the host trees, particularly their diameter at breast height (DBH). Additionally, the study suggests that Piper species are more commonly found on rare tree species, possibly due to their preference for larger host tree DBH. The results highlight the non-random association between climbers and their host trees, which is influenced by local environmental conditions. These interactions are likely to have important implications for forest vegetation dynamics and the maintenance of diversity.
Keywords:
Piper; climber; tropical forest
Available in the Digital Repository of University of South Bohemia.
Determinants of Piper (Piperaceae) climber composition in a lowland tropical rainforest in New Guinea
The study examines the distribution of root climbers from the genus Piper on host trees depending on environmental properties and host tree characteristics in a lowland primary tropical rainforest in ...
Mechanisms behind diversity and ecosystem functioning relationships - the insights from observational and manipulative studies in grassland communities
LISNER, Aleš
2023 - English
This thesis focuses on the relationship between diversity and two commonly used ecosystem functions - productivity and its stability. The thesis tries to assess the problematics from different perspectives using large-scale observational studies and small-scale manipulative experiments to better understand which mechanism create observed patterns. High emphasis is put on the methodological aspects and potential theoretical or practical issues arising from experimental setting and data collection. More specifically, the thesis starts with description and critical evaluation of patterns observed at bigger spatial scales and across multiple sites (Chapter I). Then continue with detailed small-scale experimental approaches and assessment of mechanism driving relationship between diversity, productivity and stability of communities (Chapter II and III). Further, the thesis digs into bigger detail focusing on which plant characteristics define abundance patterns of individual species, which translates into productivity (Chapter IV). Finally, it elaborates on common issues related to vegetation data collection and their subsequent analysis (Chapter V).
Keywords:
diversity; productivity; ecosystem functioning; traits; stability; sampling error
Available in the Digital Repository of University of South Bohemia.
Mechanisms behind diversity and ecosystem functioning relationships - the insights from observational and manipulative studies in grassland communities
This thesis focuses on the relationship between diversity and two commonly used ecosystem functions - productivity and its stability. The thesis tries to assess the problematics from different ...
Cell segmentation from wide-field light microscopy images using CNNs
GHAZNAVI, Ali
2023 - English
Image object segmentation allows localising the region of interest in the image (ROI) and separating the foreground from the background. Cell detection and segmentation are the primary and critical steps in microscopy image analysis. Analysing microscopy images allows us to extract vital information about the cells, including their morphology, size, and life cycle. On the other hand, living cell segmentation is challenging due to the complexity of these datasets. This research focused on developing Artificial Intelligence/Machine Learning methods of single- and multi-class segmentation of living cells. For this study, the Negroid cervical epithelioid carcinoma HeLa line was chosen as the oldest, immortal, and most widely used model cell line. Several time-lapse image series of living HeLa cells were captured using a high-resolved wide-field transmitted/reflected light microscope (custom-made for the Institute of Complex System, Nové Hrady, Czech Republic) to observe micro-objects and cells. Employing a telecentric objective with a high-resolution camera with a large sensor size allows us to achieve a high level of detail and sharper borders in large microscopy images. The collected time-lapse images were calibrated and denoised in the pre-processing step. The data sets collected under the transmission microscope setup were analyzed using a simple U-Net, Attention U-Net, and Residual Attention U-Net to achieve the best single-class semantic segmentation result. The data sets collected under the reflection microscope setup were analyzed using hybrid U-Net methods, including Vgg19-Unet, Inception-Unet, and ResNet34-Unet, to achieve the most precise multi-class segmentation result. Image object segmentation allows localising the region of interest in the image (ROI) and separating the foreground from the background. Cell detection and segmentation are the primary and critical steps in microscopy image analysis. Analysing microscopy images allows us to extract vital information about the cells, including their morphology, size, and life cycle. On the other hand, living cell segmentation is challenging due to the complexity of these datasets. This research focused on developing Artificial Intelligence/Machine Learning methods of single- and multi-class segmentation of living cells. For this study, the Negroid cervical epithelioid carcinoma HeLa line was chosen as the oldest, immortal, and most widely used model cell line. Several time-lapse image series of living HeLa cells were captured using a high-resolved wide-field transmitted/reflected light microscope (custom-made for the Institute of Complex System, Nové Hrady, Czech Republic) to observe micro-objects and cells. Employing a telecentric objective with a high-resolution camera with a large sensor size allows us to achieve a high level of detail and sharper borders in large microscopy images. The collected time-lapse images were calibrated and denoised in the pre-processing step. The data sets collected under the transmission microscope setup were analyzed using a simple U-Net, Attention U-Net, and Residual Attention U-Net to achieve the best single-class semantic segmentation result. The data sets collected under the reflection microscope setup were analyzed using hybrid U-Net methods, including Vgg19-Unet, Inception-Unet, and ResNet34-Unet, to achieve the most precise multi-class segmentation result.
Keywords:
Categorical segmentation; Neural network; Cell detection; Microscopy image segmentation; U-Net; Tissue segmentation; Semantic segmentation; Bright-Field Microscopy cell segmentation; Cell analysis
Available in the Digital Repository of University of South Bohemia.
Cell segmentation from wide-field light microscopy images using CNNs
Image object segmentation allows localising the region of interest in the image (ROI) and separating the foreground from the background. Cell detection and segmentation are the primary and critical ...
Effects of hydropeaking on the attached eggs of a rheophilic cyprinid species
BARTOŇ, Daniel
2023 - English
Ability of eggs of asp (Leuciscus aspius) to survive an increased water velocity was tested in experimental condition as well as in situ. Hydropeaking occurs on the study site and can cause detachment of most spawned eggs.
Keywords:
fish reproduction; Leuciscus aspius; wildlife conservation; river ecology; habitat alternation
Available in the Digital Repository of University of South Bohemia.
Effects of hydropeaking on the attached eggs of a rheophilic cyprinid species
Ability of eggs of asp (Leuciscus aspius) to survive an increased water velocity was tested in experimental condition as well as in situ. Hydropeaking occurs on the study site and can cause detachment ...
Detection of Diabetic Retinopathy using Deep Learning and Transfer Learning Techniques with Oversampling to Address Imbalanced Dataset
RANĐELOVIĆ, Teodora
2023 - English
The study aims to develop a system for detecting diabetic retinopathy using deep learning. In this study I have explored transfer learning with four distinct models and addressed the issue of an unbalanced dataset with oversampling. The final experiment achieved a significant improvement in accuracy and quadratic kappa score. The study highlights the potential of deep learning and the importance of addressing dataset imbalances for accurate results. The study aims to develop a system for detecting diabetic retinopathy using deep learning. In this study I have explored transfer learning with four distinct models and addressed the issue of an unbalanced dataset with oversampling. The final experiment achieved a significant improvement in accuracy and quadratic kappa score. The study highlights the potential of deep learning and the importance of addressing dataset imbalances for accurate results.
Keywords:
Diabetic Retinopathy; Deep learning; Transfer learning; Convolutional neural network; Image classification; medical imaging; diabetic macular edema; retinal fundus photographs; comparative analysis; oversampling; accuracy; quadratic kappa score
Available in the Digital Repository of University of South Bohemia.
Detection of Diabetic Retinopathy using Deep Learning and Transfer Learning Techniques with Oversampling to Address Imbalanced Dataset
The study aims to develop a system for detecting diabetic retinopathy using deep learning. In this study I have explored transfer learning with four distinct models and addressed the issue of an ...
Vampirism and Queerness in Nineteenth-Century Literature
ZEMANOVÁ, Eva
2023 - English
Cílem této práce je analýza spojení mezi vampirismem a queer motivy v dílech Drákula od Brama Stokera a Krev upírky od Florence Marryat. Diplomová práce je rozdělena do tří částí. První část se zabývá vztahem viktoriánské Anglie k sexualitě a spojitostí mezi queer motivy a gotickým románem 19. století. Věnujeme se zde rozdílnému vnímání mužské a ženské homosexuality a zobrazování tzv. "Others" v dobové literatuře. Ve druhé části se zaměříme na interpretaci díla Drákula z hlediska queer teorie, a to prostřednictvím hraběte Drákuly a role, kterou v románu představuje, včetně jeho vztahu k ostatním mužským postavám. Třetí část se soustředí na analýzu díla Krev upírky, které popisuje osud ženské upírky Harriet Brandt. Nakonec se práce zabývá aspekty jinakosti, kterými Harriet ohrožuje viktoriánskou společnost, a zároveň dvojznačným chápáním tohoto díla. The aim of this thesis is the analysis of the connection between vampirism and queerness in Bram Stoker's Dracula and Florence Marryat's The Blood of the Vampire. The Master's thesis is divided into three parts. The first part examines the relationship of Victorian England to sexuality and the connection between queerness and the 19th-century Gothic novel. We will pay attention to the different perceptions of male and female homosexuality and the portrayal of the so-called "Others" in contemporary literature. In the second part, we will focus on the interpretation of Dracula in terms of queerness, namely through Count Dracula and the role he plays in the novel, including his relations to the other male characters in the novel. The third part concentrates on the analysis of The Blood of the Vampire, describing the fate of female vampire Harriet Brandt. Finally, the thesis explores the aspects of Harriet's Otherness that Harriet threatens Victorian society with, as well as the ambiguous understanding of this work.
Keywords:
queer motivy; vampirismus; viktoriánská Anglie; Bram Stoker; Drákula; Florence Marryat; Krev upírky; společnost
Available in the Digital Repository of University of South Bohemia.
Vampirism and Queerness in Nineteenth-Century Literature
Cílem této práce je analýza spojení mezi vampirismem a queer motivy v dílech Drákula od Brama Stokera a Krev upírky od Florence Marryat. Diplomová práce je rozdělena do tří částí. První část se zabývá ...
Jane Austen's characters Elizabeth Bennet and Emma Woodhouse and their social position
HRDÁ, Linda
2023 - English
Cílem této práce je analýza společenského postavení hlavních postav v románech Jane Austenové, konkrétně Pýchy a Předsudku a Emmy. Bakalářská práce je rozdělena do čtyř kapitol. První se zabývá autorkou Jane Austenovou, jejím životem a tvorbou a následně autory, jež Austenovou ovlivnili při jejím psaní. Druhá kapitola nám přibližuje dobu, kdy autorka žila, tedy Anglii 19. století. Době se přezdívalo Regentská Anglie a přiblížíme si zde roli a rozdíly v životě žen a mužů a ekonomickou situaci, jak obecně v 19. století, tak v románech Jane Austenové. Budou uvedeny příklady, kolik vydělávali hlavní hrdinové. Třetí část už se soustředí na samotnou hlavní postavu románu Emma. Popisuje rysy, kterými se Emma liší od ostatních hrdinek ve zbytku Austenových románů. Zároveň projednává její postavení v kruhu společnosti, ve kterém se vyskytuje. Druhá podkapitola odkrývá vztah s nejbližšími postavami, se kterými Emma přišla do kontaktu. Poslední kapitola Elizabeth Bennetová má trochu jinou strukturu než předchozí, i přes to že cílem práce je hlavní hrdinky vrhnou na stejné světlo. Vymezuje limity Bennetovy rodiny, které souvisí s jejich příjmem, a tudíž limituje jejich společenské postavení ve společnosti. V poslední kapitole je zahrnuta i Elizabetina ženskost. Na závěr práce porovnávám naše dvě hrdinky, konkrétně jejich osobnost, pohled na manželství, rozvoj charakteru a sociální postavení. This thesis aims to analyze the social status of the main characters in Jane Austen's novels, specifically Pride and Prejudice and Emma. The bachelor thesis is divided into four chapters. The first deals with the author Jane Austen, her life and work, and then focus on the authors who influenced Austen in her writing. The second chapter brings us closer to the time when the author lived in England in the 19th century. The era was called the Regency Era and we going to look at the role and differences in the lives of women and men and the economic situation, both in general in the 19th century and in Jane Austen's novels. Examples of how much the main male characters earned going to be given. The third part finally focuses on the main character of the novel, Emma. It describes the features that make Emma different from the other heroines in the rest of Austen's novels, while also discussing her position in the circle of society in which she existed. The second sub-chapter reveals the relationship with the closest characters with whom Emma came into contact. The last chapter of Elizabeth Bennet has a slightly different structure than the previous one, even though the work tries to cast the main characters in the same light. It defines the limits of the Bennet family, which is related to their income and therefore limits their social status in society. Elizabeth's womanhood is also included in the last chapter. At the end of the work, I compare our two heroines, specifically their personalities, views on marriage, character development, and social status.
Keywords:
ženské postavení; Jane Austenová; regentská Anglie; Elizabeth Bennet; Pýcha a předsudek; Emma Woodhouse; Emma; společnost v 18. a 19. století
Available in the Digital Repository of University of South Bohemia.
Jane Austen's characters Elizabeth Bennet and Emma Woodhouse and their social position
Cílem této práce je analýza společenského postavení hlavních postav v románech Jane Austenové, konkrétně Pýchy a Předsudku a Emmy. Bakalářská práce je rozdělena do čtyř kapitol. První se zabývá ...
The Reception of Harriet Jacobs' Incidents in the Life of a Slave Girl
HOUŠKOVÁ, Barbora
2023 - English
Cílem této bakalářské práce je zaměřit se na problematiku recepce díla Incidents in the Life of a Slave Girl od afroamerické autorky Harriet Jacobs. Ačkoli je tato autobiografie, poprvé vydaná počátkem roku 1861, dnes známá jako jedno z nejpřednějších děl tzv. slave narratives, vyprávění otroků, jeho autenticita a autorství byly zpochybňovány po mnoho dekád, a teprve koncem 20. století byla kniha opět uznána jako hodnotné a pravdivé osobní svědectví toho, co pro afroamerické ženy znamenalo otroctví ve Spojených státech před občanskou válkou. Tato práce se tedy soustředí jak na recepci dobovou, s přihlédnutím především k historickým okolnostem abolicionismu a začátku občanské války, tak i na recepci současnou, zejména na kritické ohlasy 20. století a následnou kanonizaci díla. Klíčovým faktorem této analýzy bude mnohonásobná diskriminace, jež ovlivňovala život i práci Harriet Jacobs jakožto sexuálně zneužívané ženy afroamerického původu. The aim of this thesis is to explore the problematics of the reception of Harriet Jacobs' Incidents in the Life of a Slave Girl. Though this autobiography, first published at the beginning of 1861, is today known as one of the most major works of the genre of slave narratives, its authenticity and authorship have been questioned and denied for several decades, and only by the end of the 20th century has the narrative been recognized as a valuable and truthful personal testimony of what slavery meant for African American women in antebellum America. This thesis then focuses on both the contemporary reception, mainly with regard to the historical circumstances of abolitionism and the upcoming Civil War, and a more recent one, predominantly on 20th-century criticism and the subsequent canonization of the narrative. The key factor of this analysis will be the multiple discrimination which affected the life and work of Harriet Jacobs as a sexually abused woman of African American descent.
Keywords:
abolicionismus; otroctví; autobiografie; vyprávění otroků; intersekcionalita; gender
Available in the Digital Repository of University of South Bohemia.
The Reception of Harriet Jacobs' Incidents in the Life of a Slave Girl
Cílem této bakalářské práce je zaměřit se na problematiku recepce díla Incidents in the Life of a Slave Girl od afroamerické autorky Harriet Jacobs. Ačkoli je tato autobiografie, poprvé vydaná ...
Sperm maturation process in fishes (review)
KŘIŠŤAN, Jakub
2023 - English
Hlavní cíl této práce bylo posouzení aktuálních informací v oboru zrání spermatu (procesu, při kterém nezralé spermie získají potenciál reagovat na faktory podněcující pohyblivost spermií) a také posouzení struktury urogenitálního systému ryb v souvislosti se zráním spermií. The main aim of this thesis was to review the current knowledge in the field of fish sperm maturation (a process where immature spermatozoa acquire the potential to respond to motility-activating factors) and the urogenital system structure in relation to this maturation.
Keywords:
zrání spermií; pohyblivost spermií; spermatogeneze; spermie; urogenitální systém; spermiace; endokrinní systém; hormonální stimulace; varlata; testikulární sperma; akvakultura; Chondrostei; Teleostei; Chondrichthyes
Available in the Digital Repository of University of South Bohemia.
Sperm maturation process in fishes (review)
Hlavní cíl této práce bylo posouzení aktuálních informací v oboru zrání spermatu (procesu, při kterém nezralé spermie získají potenciál reagovat na faktory podněcující pohyblivost spermií) a také ...
NRGL provides central access to information on grey literature produced in the Czech Republic in the fields of science, research and education. You can find more information about grey literature and NRGL at service web
Send your suggestions and comments to nusl@techlib.cz
Provider
Other bases