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Study of DNA oligonucleotides interactions with ethidium bromide by partial-filling affinity capillary electrophoresis
Růžička, Martin; Koval, Dušan; Kašička, Václav
2014 - anglický
A partial filling affinity capillary electrophoresis was developed and applied to investigation of non-covalent molecular interactions between double stranded DNA oligonucleotide (Dickerson dodecamer) and classical DNA intercalator ligand –ethidium bromide (EtBr). Binding constants of DNA EtBr complexes were determined from the dependence of migration time changes of DNA oligomer (applied as analyte) on the length of ligand zones introduced beforehand as plugs of various lengths in hydroxypropylcellulose coated fused silica capillary. Binding constants of DNA-EtBr complex were found to be in the range 4.2 x 103 – 1.5 104 L/mol.
Klíčová slova:
affinity capillary electrophoresis; binding constant; oligonucleotide
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Study of DNA oligonucleotides interactions with ethidium bromide by partial-filling affinity capillary electrophoresis
A partial filling affinity capillary electrophoresis was developed and applied to investigation of non-covalent molecular interactions between double stranded DNA oligonucleotide (Dickerson dodecamer) ...
Determination of enantiopurity of new types of acyclic nucleoside phosphonates by capillary electrophoresis with cyclodextrins-based chiral selectors
Šolínová, Veronika; Kaiser, Martin Maxmilian; Lukáč, Miloš; Janeba, Zlatko; Kašička, Václav
2014 - anglický
Capillary electrophoresis with neutral and cationic cyclodextrins as chiral selectors was applied for determination of enantiopurity analysis of new types of six acyclic nucleoside phosphonates, nucleotide analogues bearing ((3-hydroxypropan-2-yl)-1H-1,2,3-triazol-4-yl)phosphonic acid, 2-((diisopropoxyphosphonyl)methoxy)propanoic acid or 2 (phosphonomethoxy)propanoic acid moieties attached to adenine, guanine, 2,6-diaminopurine, uracil and 5-bromouracil nucleobases. All these compounds were found to be synthesized in pure enantiomeric forms. Employing the UV-absorption detection at 206 nm, their detection limits were in the low micromolar level.
Klíčová slova:
acyclic nucleoside phosphonates; capillary electrophoresis; enantiopurity analysis
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Determination of enantiopurity of new types of acyclic nucleoside phosphonates by capillary electrophoresis with cyclodextrins-based chiral selectors
Capillary electrophoresis with neutral and cationic cyclodextrins as chiral selectors was applied for determination of enantiopurity analysis of new types of six acyclic nucleoside phosphonates, ...
Novel conformationally locked nucleosides and nucleotides
Nencka, Radim; Šála, Michal; Hřebabecký, Hubert; Procházková, Eliška; Mackman, R.; Barauskas, O.; Lee, Y. J.; Tian, Y.
2014 - anglický
We report on a synthesis of two novel types of conformationally locked nucleoside analogues. We prepared 7 membered ring 3’,5’-bridged nucleoside analogs locked in a Southern conformation. These nucleoside analogues were converted in one pot to appropriate phosphoramidate prodrugs using boronate methodology. We also devised and prepared a novel carbocyclic nucleoside by replacing furanose with cyclohexene that resulted in a locked Northern conformation similar to LNA monomers. Further details on the biological activity and application for oligonucleotide synthesis will be discussed in detail.
Klíčová slova:
locked nucleoside analogues; oligonucleotide synthesis
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Novel conformationally locked nucleosides and nucleotides
We report on a synthesis of two novel types of conformationally locked nucleoside analogues. We prepared 7 membered ring 3’,5’-bridged nucleoside analogs locked in a Southern conformation. These ...
Structure of an immunoactive polysaccharide isolated from Korean mulberry fruit (Morus alba L.)
Bleha, R.; Lee, J. S.; Capek, P.; Pohl, Radek; Kim, H. B.; Choi, D. J.; Lee, S.; Lee, J.; Jang, S. J.; Synytsya, A.; Park, Y. I.
2014 - anglický
A water-soluble polysaccharide (JS-MP-1) was isolated from the water extract of Korean white mulberry fruits (Morus alba L.) by ethanol precipitation and then purified by DEAE-cellulose ion exchange chromatography. Obtained final polysaccharide (1600 KDa) consisted of galactose, arabinose and rhamnose as major neutral sugars. JS-MP-1 also contains galacturonic and glucuronic acids (4:1). Sugar linkage, FTIR and correlation NMR analyses confirmed that it is a rhamnogalacturonan type I (RG I), which contains the alternating sequence of 1,4-alpha-D-GalAp and 1,2-alpha-L-Rhap units in the backbone. Neutral sugar side chains of JS-MP1 were identified as (1 -> 5)-alpha-L-arabinan and arabinogalactan type II (AG II) having the (1 -> 6)-beta-D-galactan core. The arabinan side chains are bound to the backbone at the O-4 position of some alpha-L-Rhap units, while the way of linkage between RG I and AG II chains is unclear. It was demonstrated that JS-MP-1 significantly stimulates murine macrophage RAW 264.7 cells to release chemokines (RANTES and MIP-1 alpha) and proinflammatory cytokines like INF-alpha and IL-6, and induce the iNOS and COX-2 gene expression, which are responsible for the production of NO and prostaglandin PGE2, respectively. These results suggest that JS-MP-1 can act as a potent immunomodulator and these observations may support the applicability of this polysaccharide or the water extracts of mulberry fruit can be used as an immunotherapeutic adjuvant or health beneficial food material.
Klíčová slova:
pectic polysaccharides
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Structure of an immunoactive polysaccharide isolated from Korean mulberry fruit (Morus alba L.)
A water-soluble polysaccharide (JS-MP-1) was isolated from the water extract of Korean white mulberry fruits (Morus alba L.) by ethanol precipitation and then purified by DEAE-cellulose ion exchange ...
Development and applications of ionization techniques in ambient mass spectrometry
Rejšek, Jan; Vrkoslav, Vladimír; Cvačka, Josef
2014 - anglický
Ambient mass spectrometry is a sharply developing subject area. Desorption electrospray ionization (DESI) and desorption atmospheric pressure photoionization (DAPPI) are two types of ionization techniques in the ambient mass spectometry. Herein, they were investigated in two research areas: (1) TLC analysis. (2) Localization of insect defense glands.
Klíčová slova:
ambient mass spectrometry; desorption electrospray ionization; desorption atmospheric pressure photoionization; thin-layer chromatography; insect defense compounds; mass spectrometry imaging
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Development and applications of ionization techniques in ambient mass spectrometry
Ambient mass spectrometry is a sharply developing subject area. Desorption electrospray ionization (DESI) and desorption atmospheric pressure photoionization (DAPPI) are two types of ionization ...
Analysis and characterization of antimicrobial peptides by capillary electromigration methods
Tůmová, Tereza; Monincová, Lenka; Čeřovský, Václav; Kašička, Václav
2014 - anglický
High-performance capillary electromigration methods (zone electrophoresis, affinity electrophoresis, isotachophoresis and micellar electrokinetic chromatography) have been applied to analysis and physicochemical characterization of halictines – antimicrobial peptides isolated from the bee Halictus sexcinctus.
Klíčová slova:
antimicrobial peptides; capillary electrophoresis; coating
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Analysis and characterization of antimicrobial peptides by capillary electromigration methods
High-performance capillary electromigration methods (zone electrophoresis, affinity electrophoresis, isotachophoresis and micellar electrokinetic chromatography) have been applied to analysis and ...
Analytical methods Used in the Studies on Chemical Ecology of Termites
Krasulová, Jana; Hanus, Robert; Sillam-Dusses, David; Žáček, Petr; Jiroš, Pavel; Šobotník, Jan; Kalinová, Blanka; Bosáková, Z.; Valterová, Irena
2012 - anglický
In this paper, I review our recent results on the trail-following communication in the Neotropical termite Glossotermes oculas (Serritermitidae) and the defensive chemistry of the African termite Psammotermes hybostoma (Rhinotermitidae). These findings belong to our current project focused on chemical ecology of critical termite genera from families Rhinotermitidae and Serritermitidae.
Klíčová slova:
defensive compounds; GC-MS; GCxGC-MS; termites; trail-following pheromone
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Analytical methods Used in the Studies on Chemical Ecology of Termites
In this paper, I review our recent results on the trail-following communication in the Neotropical termite Glossotermes oculas (Serritermitidae) and the defensive chemistry of the African termite ...
Photoluminiscent diamond nanoparticles - new diagnostic and visualization probes
Cígler, Petr
2012 - anglický
Selected problems connected with surface modification of nanosized diamond crystals, attachment of chemical sensing architectures to them and their colloidal stability were addressed. Fluorescent nanodiamonds modified by proper functional structures were shown as unique unbleachable nanoprobes for construction of future sensors useful in biological and medicinal applications.
Klíčová slova:
nanodiamond; fluorescence probe; nanosensor
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Photoluminiscent diamond nanoparticles - new diagnostic and visualization probes
Selected problems connected with surface modification of nanosized diamond crystals, attachment of chemical sensing architectures to them and their colloidal stability were addressed. Fluorescent ...
Influence of the O-phosphorylation of serine, threonine and tyrosine in proteins on the amidic N-15 chemical shielding anisotropy tensors
Emmer, J.; Vavrinská, A.; Sychrovský, Vladimír; Benda, Ladislav; Kříž, Z.; Koča, J.; Boelens, R.; Sklenář, V.; Trantírek, L.
2012 - anglický
Density functional theory was employed to study the influence of O-phosphorylation of serine, threonine, and tyrosine on the amidic N-15 chemical shielding anisotropy (CSA) tensor in the context of the complex chemical environments of protein structures.
Klíčová slova:
15N CSA; protein phosphorylation; DFT
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Influence of the O-phosphorylation of serine, threonine and tyrosine in proteins on the amidic N-15 chemical shielding anisotropy tensors
Density functional theory was employed to study the influence of O-phosphorylation of serine, threonine, and tyrosine on the amidic N-15 chemical shielding anisotropy (CSA) tensor in the context of ...
Molecular simulations unravel preference of haloalkanes for the air/water interface
Habartová, Alena; Minofar, Babak; Obisesan, A.; Roeselová, Martina
2012 - anglický
Halogenated organics are emitted into the atmosphere from a variety of sources of both natural and anthropogenic origin (Finlayson-Pitts et al., 2000). Their uptake at the surface of aerosols can affect their reactivity, for example in processes which take part in ozone destruction due to production of reactive chlorine, bromine and iodine radicals. This study presents results of molecular dynamic (MD) simulations carried out to investigate the interaction of short-chain haloalkanes with aqueous surfaces. We report on a free energy minimum that the haloalkanes exhibit at the air/water interface.
Klíčová slova:
haloalkanes; aqueous solvation; molecular dynamics simulations
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Molecular simulations unravel preference of haloalkanes for the air/water interface
Halogenated organics are emitted into the atmosphere from a variety of sources of both natural and anthropogenic origin (Finlayson-Pitts et al., 2000). Their uptake at the surface of aerosols can ...
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