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No (118)
Number of found documents:
282
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Development of microfluidic tools for cell analysis
Václavek, Tomáš; Křenková, Jana; Foret, František
2015 - English
In this poster we present our microfabrication approach for fabrication of glass microfluidic devices which we combine with 3D-printed parts in order to develop an easy-to-use instrumentation for single cell analysis.
Keywords:
microfluidic device; 3D-printing; single cell analysis
Fulltext is available at external website.
Development of microfluidic tools for cell analysis
In this poster we present our microfabrication approach for fabrication of glass microfluidic devices which we combine with 3D-printed parts in order to develop an easy-to-use instrumentation for ...
Determination of biological active compounds in pressurized water extract of Sambucus nigra L. branches
Hohnová, Barbora; Šalplachta, Jiří
2015 - English
GC-MS and SDS-PAGE methods were employed for determination of carbohydrates and biological active proteins in pressurized hot water extract of Sambucus nigra L. branches.
Keywords:
PHWE; GC-MS; SDS-PAGE
Fulltext is available at external website.
Determination of biological active compounds in pressurized water extract of Sambucus nigra L. branches
GC-MS and SDS-PAGE methods were employed for determination of carbohydrates and biological active proteins in pressurized hot water extract of Sambucus nigra L. branches.
Simple route of caspase-3 FRET sensor synthesis using “click chemistry”
Lišková, Marcela; Křenková, Jana; Klepárník, Karel; Pazdera, P.; Foret, František
2015 - English
Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences Asp-Glu-Val-Asp (DEVD) on their substrates and hydrolyze peptide bonds after aspartic acid residues. Various techniques for the determination of caspase-3 are commercially available e.g. Enzyme Linked Immuno-Sorbent Assay (ELISA), Western blotting or flow cytometric analysis. The products of the cleavage can be detected by spectrophotometry, fluorimetry, chemiluminescence (CL) or ELISA. In this work, we suggested fluorescent sensor based on easily prepared Förster Resonance Energy Transfer (FRET). We use very simple chemistry called “click”. This type of chemistry takes advantages of quickness, simplicity and cheapness. “Click chemistry” is based on usage of various functional group and cross-linkers to combine individual molecules together.
Keywords:
caspases; click chemistry; cell analysis
Fulltext is available at external website.
Simple route of caspase-3 FRET sensor synthesis using “click chemistry”
Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences ...
Low-cost 3D printed device for fabrication of nanoESI tips by grinding
Přikryl, Jan; Týčová, Anna; Foret, František
2015 - English
The aim of this work was the development of a simple and low-cost device for nanospray tips fabrication. Although there are several ways of the tip preparation, grinding was chosen as the most suitable in this work. Inexpensive, flexible and reliable method of 3D printing was used for the device construction making it available for any research group with a 3D printer.
Keywords:
3D printing; grinding; nanospray tip
Fulltext is available at external website.
Low-cost 3D printed device for fabrication of nanoESI tips by grinding
The aim of this work was the development of a simple and low-cost device for nanospray tips fabrication. Although there are several ways of the tip preparation, grinding was chosen as the most ...
Quantum dot-based immunoprobe for optical and electrochemical detection
Dvořáková, V.; Čadková, M.; Datinská, Vladimíra; Chałupniak, A.; Korecká, L.; Merkoci, A.; Klepárník, Karel; Foret, František; Bílková, Z.
2015 - English
Three-stage labeling strategy allows the preparation of specific conjugates composed of required antibodies and quantum dots as an extremely sensitive bio-probe applicable in varied immunoassays with following optical and electrochemical detection. This approach is essentially based on carbodiimide chemistry however each single step of whole procedure is separated and controlled which makes this applied protocol oriented, highly efficient and it enables gain of pure antibody-conjugated quantum dots in ready-to-use condition.
Keywords:
quantum dots; conjugation; carbodiimide coupling; IgG molecules
Fulltext is available at external website.
Quantum dot-based immunoprobe for optical and electrochemical detection
Three-stage labeling strategy allows the preparation of specific conjugates composed of required antibodies and quantum dots as an extremely sensitive bio-probe applicable in varied immunoassays with ...
Study of the cell wall of Staphylococcus aureus and its sensitivity to enzybiotics
Čmelík, Richard; Melková, K.; Kobzová, Š.; Janda, L.
2015 - English
The endolysin resistant and sensitive strains of Staphylococcus aureus were compared by means of LC-MS based structural analysis of peptidoglycan isolated from their cell walls. The structural explanation of the resistance was suggested.
Keywords:
peptidoglycan; LC-MS; enzybiotics
Fulltext is available at external website.
Study of the cell wall of Staphylococcus aureus and its sensitivity to enzybiotics
The endolysin resistant and sensitive strains of Staphylococcus aureus were compared by means of LC-MS based structural analysis of peptidoglycan isolated from their cell walls. The structural ...
Simple flow-focusing microfluidic chip for droplet generation
Křivánková, Jana; Basova, E. Y.; Foret, František
2015 - English
There is an ascending requirement for analysis of chemical content of individual cells involving combination of various bioanalytical and optical sensors and arrangements. At the beginning of the cell analysis there is a need for creation of suitable transport vessels - droplets - that can be easily counted, sorted and serve for manipulation of cells with preserved biocompability as well. Among all of bioanalytical approaches droplet-based microfluidics proved its potential for producing droplets with precisely controlled size, shape as well as for encapsulation of reactants and delivering drugs. This study presents a simple microfluidic chip with flow-focusing geometry for generation of water droplets in fluorocarbon oil.
Keywords:
microfluidic chip; droplet generation; fluorinated oil phase
Fulltext is available at external website.
Simple flow-focusing microfluidic chip for droplet generation
There is an ascending requirement for analysis of chemical content of individual cells involving combination of various bioanalytical and optical sensors and arrangements. At the beginning of the cell ...
Efficient generation of arsanes coupled with HPLC or with cryotrapping for speciation analysis of arsenic
Marschner, Karel; Musil, Stanislav; Rychlovský, P.; Dědina, Jiří
2015 - English
Arsenic speciation analysis by HPLC postcolumn hydride generation or with cryotrapping and detection by atomic fluorescence spectrometry.
Keywords:
arsenic speciation analysis; hydride generation; HPLC
Available in digital repository of the ASCR
Efficient generation of arsanes coupled with HPLC or with cryotrapping for speciation analysis of arsenic
Arsenic speciation analysis by HPLC postcolumn hydride generation or with cryotrapping and detection by atomic fluorescence spectrometry.
Comparison of ionisation properties of aetma-labeled saccharides with common labels
Partyka, Jan; Foret, František
2015 - English
We have tested (2-aminoethyl)trimethylammonium (AETMA) as a label for analysis of oligosaccharides by capillary electrophoresis with electrospray (ESI) mass spectrometry detection and compared its performance to taurine, 2-aminobenzoic acid, 2-aminobenzamide and 8-aminopyrene-1,3,6-trisulfonic acid. The AETMA-labeled saccharides were provided higher ionization signals in positive mode than native sodium or ammonium adducts and equal or higher signals than saccharides labeled by the more commonly used labels tested in this study.
Keywords:
saccharides; AETMA; ESI/MS
Fulltext is available at external website.
Comparison of ionisation properties of aetma-labeled saccharides with common labels
We have tested (2-aminoethyl)trimethylammonium (AETMA) as a label for analysis of oligosaccharides by capillary electrophoresis with electrospray (ESI) mass spectrometry detection and compared its ...
System for fast analysis of explosives in the enviromnemt
Čapka, Lukáš; Večeřa, Zbyněk; Mikuška, Pavel; Šesták, Jozef; Kahle, Vladislav
2015 - English
Detection of explosives is the subject of many investigations by law enforcement agents and forensic scientist. The identification and discrimination of evidence taken from crime scenes is a common practice in forensic investigations. The higher demands on simple and fast determination of hazard materials or strange objects are required in connection with worldwide often repeated terrorist activities.
Keywords:
explosives; hazard materials; energetic materials; portable device for the analysis
Fulltext is available at external website.
System for fast analysis of explosives in the enviromnemt
Detection of explosives is the subject of many investigations by law enforcement agents and forensic scientist. The identification and discrimination of evidence taken from crime scenes is a common ...
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