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New developments in hydride atomizers for atomic absorption and atomic fluorescence spectrometry
Dědina, Jiří; Korunová, Vlasta; D'Ulivo, A.
2003 - anglický
The most recent developments of multiatomizers and FIFS were described and performance of the atomizers namely in terms of resistance to atomization interferences and of minimization of the limit detection for AAS and AFS was discussed.
Klíčová slova:
atomic absortion spectrometry; hydride atomization; hydride generation
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
New developments in hydride atomizers for atomic absorption and atomic fluorescence spectrometry
The most recent developments of multiatomizers and FIFS were described and performance of the atomizers namely in terms of resistance to atomization interferences and of minimization of the limit ...
Dynamic modification of microorganisms by pyrene-butanoate for fluorometric detection in capillary electrokinetic techniques
Horká, Marie; Růžička, F.; Šlais, Karel
2003 - anglický
Pyrenebutanoate and non-ionogenic tenside on the basis of pyrenebutanoate as the fluorescent compounds were used as buffer additives in capillary zone electrophoresis or capillary isoelectric focusing, respectively, for a dynamic modification of the microbial samples. Using the deuterium lamp UV excitation for the on column fluorometric detection, the minimum detectable amount of the microorganisms in order of ones to tens of cells sampled on the separation capillary was achieved.
Klíčová slova:
capillary electrokinetic techniques; dynamic modification of microorganisms; fluorometric detection
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Dynamic modification of microorganisms by pyrene-butanoate for fluorometric detection in capillary electrokinetic techniques
Pyrenebutanoate and non-ionogenic tenside on the basis of pyrenebutanoate as the fluorescent compounds were used as buffer additives in capillary zone electrophoresis or capillary isoelectric ...
Preparation and optimization of packing of microcolumns for HPLC and SFC
Planeta, Josef
2003 - anglický
The lecture summarizes the study of the influence of packing conditions on the efficiency and the total porosity of the prepared columns. For both critical and supercritical CO2, optimum packing conditions were found.and their reproducibility was defined.
Klíčová slova:
packing microcolumns; preparation
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Preparation and optimization of packing of microcolumns for HPLC and SFC
The lecture summarizes the study of the influence of packing conditions on the efficiency and the total porosity of the prepared columns. For both critical and supercritical CO2, optimum packing ...
Autoradiography of 75Se on Image Plate
Korunová, Vlasta
2002 - anglický
The Image Plate and BAS5000 Fuji Film instrument were used to depict a flat layout of 75Se and for its quantitative determination. The goal for this experiment was determinig the efficiency and area of selenium deposited during in situ trapping in the AAS graphite furnace. S graphite furnace.
Klíčová slova:
autoradiography; 75Se isotop; image plate
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Autoradiography of 75Se on Image Plate
The Image Plate and BAS5000 Fuji Film instrument were used to depict a flat layout of 75Se and for its quantitative determination. The goal for this experiment was determinig the efficiency and area ...
Chiral separation of charged cluster boranes
Slavíček, V.; Vespalec, Radim
2002 - anglický
Capillary zone electrophoresis is effective instrumental technique for chiral separations of charged cluster boranes if β-cyclodextrin serves as chiral selector.
Klíčová slova:
capillary electrophoresis; chiral separation; cluster boranes; borany; odlučování
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Chiral separation of charged cluster boranes
Capillary zone electrophoresis is effective instrumental technique for chiral separations of charged cluster boranes if β-cyclodextrin serves as chiral selector.
Single cell diagnostics
Klepárník, Karel
2002 - anglický
DNA fragmentation, as a hallmark of apoptosis, has been detected in a single MCF7 breast cancer apoptotic cell.
Klíčová slova:
single cell analysis; apoptosis
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Single cell diagnostics
DNA fragmentation, as a hallmark of apoptosis, has been detected in a single MCF7 breast cancer apoptotic cell.
pK determination of sparingly soluble weak bases
Vlčková, Markéta; Barták, P.; Vespalec, Radim
2002 - anglický
pK values of sanguinarine and chelerythrine have been determinedat conditions of planned interaction measurements and renasons for the pread of published pK data of these weak bases have been investigated.
Klíčová slova:
capillary electrophoresis; sanguinarine; chelerythrine; zásady; elektroforéza
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
pK determination of sparingly soluble weak bases
pK values of sanguinarine and chelerythrine have been determinedat conditions of planned interaction measurements and renasons for the pread of published pK data of these weak bases have been ...
Exact solution to the Navier-Stokes equation describing the velocity profile of electroosmotic flow in cylindrical capillaries
Otevřel, Marek; Klepárník, Karel
2002 - anglický
The partial differential equation describing unsteady EOF velocity profile was derived and solved. The steady state EOF was solved for an electrolyte of nonconstant viscosity.
Klíčová slova:
electroosmotic flow; Navier-Stokes equation
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Exact solution to the Navier-Stokes equation describing the velocity profile of electroosmotic flow in cylindrical capillaries
The partial differential equation describing unsteady EOF velocity profile was derived and solved. The steady state EOF was solved for an electrolyte of nonconstant viscosity.
Determination of saccharides in beverages by capillary electrophoresis and MALDI-TOF mass spectrometry
Žídková, Jitka; Přibyla, Lubomír; Chmelík, Josef
2001 - anglický
A fast method for the detection of addition of cheap sweeteners in beverages based on combination of matrix assisted laser desorption/ionisation time of flight (MALDI-TOF) mass spectrometry with capillary electrophoresis has been developed. Capillary electrophoresis has been employed to detect the ratio of concentration of major saccharides. Application of these techniques to orange juices is presented.
Klíčová slova:
saccharides; capillary electrophoresis; MALDI-TOF MS
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Determination of saccharides in beverages by capillary electrophoresis and MALDI-TOF mass spectrometry
A fast method for the detection of addition of cheap sweeteners in beverages based on combination of matrix assisted laser desorption/ionisation time of flight (MALDI-TOF) mass spectrometry with ...
Dynamic modification of proteins for fluorometric detection in CZE
Horká, Marie; Šlais, Karel
2001 - anglický
The separation techniques employing fluorescence detection are sensitive and selective so they have been often applied for the trace analysis of biological samples. The commonly used derivatization of proteins can lower the detection limits; however, they can change the acido-basic properties and mobilities when compared to the native species. Recently, we have used the colored tenside as a buffer additive for the photometric detection of proteins in the UV region. The selectivity, efficiency and resolution of CZE separation using this dye were found to be similar to the CZE with SDS as the additive. In this study, the ampiphilic fluorescent compound is suggested as a buffer additive in CZE for dynamic modification of the sample of several proteins. Using the deuterium lamp for the excitation in the UV region for the on column fluorometric detection, the amol minimum detectable amounts of the proteins sampled on the CZE capillary were achieved.
Klíčová slova:
capillary zone electrophoresis; fluorometric detection; dynamic modification
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Dynamic modification of proteins for fluorometric detection in CZE
The separation techniques employing fluorescence detection are sensitive and selective so they have been often applied for the trace analysis of biological samples. The commonly used derivatization of ...
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