Standards for capillary isoelectric focusing with laser induced fluorescence detection
Šlais, Karel; Nováčková, J.; Friedl, Z.
2001 - English Proteins, peptides or BGE ampholytes labeled with suitable dyes were previously suggested as pI markers. However, both proteins and background ampholytes have many possible reactive sites for reactive dyes and this might result in formation of heterogeneously labeled product with respect to pI. By contrast, the low molecular pI markers can be prepared with heterogeneity and greater stability than proteins and could potentially be good pI markers as found in case of UV detection. To improve the laser induced fluorescence detection, new low molecular fluorescent compounds excitable around 490 nm with suitable acidobasic and electrophoretic properties were prepared and focused in capillary IEF with photometric or fluorometric detection. The experimental setup of IEF and properties of new laser induced fluorescent pI markers are given. Keywords: capillary isoelectric focusing; standards for cIEF; laser induced fluorescent detection Available at various institutes of the ASCR Standards for capillary isoelectric focusing with laser induced fluorescence detection

Proteins, peptides or BGE ampholytes labeled with suitable dyes were previously suggested as pI markers. However, both proteins and background ampholytes have many possible reactive sites for reactive ...

Šlais, Karel; Nováčková, J.; Friedl, Z.
Ústav analytické chemie, 2001

Impact of sample matrix on quantitation in CZE
Gebauer, Petr; Křivánková, Ludmila; Pantůčková, Pavla; Boček, Petr
2001 - English Correct conditions for quantification based on peak height evaluation when UV detection is used were defined enabling to reach as high sensitivity as possible. Keywords: capillary zone electrophoresis; trace analysis; calibration curves Available at various institutes of the ASCR Impact of sample matrix on quantitation in CZE

Correct conditions for quantification based on peak height evaluation when UV detection is used were defined enabling to reach as high sensitivity as possible.

Gebauer, Petr; Křivánková, Ludmila; Pantůčková, Pavla; Boček, Petr
Ústav analytické chemie, 2001

Microchips and MS coupling
Foret, František
2001 - English Review of the current state of system miniaturization and coupling of chips with mass spectrometry. Keywords: miniaturization; mass spectrometry; proteomics Available at various institutes of the ASCR Microchips and MS coupling

Review of the current state of system miniaturization and coupling of chips with mass spectrometry.

Foret, František
Ústav analytické chemie, 2001

Chiral separations of charged boranes and carboranes by CZE
Slavíček, Viktor; Vespalec, Radim
2001 - English The applicability of capillary zone electrophoresis for achiral and chiral analysis of deltahedral boranes was proved. Twelve derivatives of different structural types were chirally separated with ß-cyclodextrin as a chiral selector. Keywords: deltahedral boranes; capillary zone electrophoresis; beta-cyclodextrin; borany; elektroforéza Available at various institutes of the ASCR Chiral separations of charged boranes and carboranes by CZE

The applicability of capillary zone electrophoresis for achiral and chiral analysis of deltahedral boranes was proved. Twelve derivatives of different structural types were chirally separated with ...

Slavíček, Viktor; Vespalec, Radim
Ústav analytické chemie, 2001

Determination of biologically active compounds in medical plants by capillary electrochromatography
Kvasničková, L.; Štěrbová, H.; Glatz, O.; Kahle, Vladislav; Slanina, J.
2001 - English Capillary electrochromatography in macroporous polyacrylamide columns was successfully used to analyse and quantify lignans in extract of the seeds of Schisandra chinensis. Good separations were achieved in less than 35 min. The levels of schizandrin, gomisin A, gomisin N and wuweizisu C determined by CEC were in good agreement with those obtained by reverse-phase HPLC. Keywords: capillary electrochromatography; lignans; Schisandra chinensis Available at various institutes of the ASCR Determination of biologically active compounds in medical plants by capillary electrochromatography

Capillary electrochromatography in macroporous polyacrylamide columns was successfully used to analyse and quantify lignans in extract of the seeds of Schisandra chinensis. Good separations were ...

Kvasničková, L.; Štěrbová, H.; Glatz, O.; Kahle, Vladislav; Slanina, J.
Ústav analytické chemie, 2001

Proteomics of barley proteins by combination of in-gel digestion and MALDI-TOF MS
Řehulka, Pavel; Chmelík, Josef; Mayrhofer, C.; Allmaier, G.
2001 - English It is shown that combination of gel electrophoresis, matrix assisted laser desorption/ionization time-of-flight mass spectrometry and database searching is a suitable procedure for rapid protein identification. This approach let us to identify some proteins from barley leaves and grains. Identification of barley proteins can be useful for characterization of varieties of malting barley which is important especially in food and brewing industry. Keywords: proteomics; protein identification; barley; ječmen Available at various institutes of the ASCR Proteomics of barley proteins by combination of in-gel digestion and MALDI-TOF MS

It is shown that combination of gel electrophoresis, matrix assisted laser desorption/ionization time-of-flight mass spectrometry and database searching is a suitable procedure for rapid protein ...

Řehulka, Pavel; Chmelík, Josef; Mayrhofer, C.; Allmaier, G.
Ústav analytické chemie, 2001

Influence of the laser power and analyte concentration on the results in MALDI-TOF MS of oligosaccharides
Čáslavský, Josef; Chmelík, Josef
2000 - English MALDI-TOF MS has been successfully applied for the analysis of a wide range of organic biopolymers including oligosaccharides. We focus our attention to the group of oligosaccharides occuring in beer and in malting and mashing products, which are mainly oligosaccharides containing from 3 to more than 30 glucose units in their molecules. We tried to evaluate various factors influencing the obtained results. We found that the shape of the spectrum depends significantly on the energy of ionising laser pulse and also on the amount of the analyte on the MALDI target. These effects were studied with standard mixtures as well as with real samples. Keywords: mass spectrometry; MALDI-TOF; oligosaccharides; hmotnostní spektrometrie; oligosacharidy Available at various institutes of the ASCR Influence of the laser power and analyte concentration on the results in MALDI-TOF MS of oligosaccharides

MALDI-TOF MS has been successfully applied for the analysis of a wide range of organic biopolymers including oligosaccharides. We focus our attention to the group of oligosaccharides occuring in beer ...

Čáslavský, Josef; Chmelík, Josef
Ústav analytické chemie, 2000

Dávkování pevných látek
Dočekal, Bohumil
1997 - English Available at various institutes of the ASCR Dávkování pevných látek

Dočekal, Bohumil
Ústav analytické chemie, 1997

Thermal decomposition of organic substances
Adamec, L.; Bartlová, M.; Coufal, O.; Teplý, Jiří
1996 - English Keywords: analýza; látky Available at various institutes of the ASCR Thermal decomposition of organic substances

Adamec, L.; Bartlová, M.; Coufal, O.; Teplý, Jiří
Ústav analytické chemie, 1996

Capillary electrophoresis in glycobiology
Plocek, Jiří; Chmelík, Josef
1996 - English Keywords: elektroforéza; umění Available at various institutes of the ASCR Capillary electrophoresis in glycobiology

Plocek, Jiří; Chmelík, Josef
Ústav analytické chemie, 1996