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Subcellular insight into cholesterol-mediated proliferation of the tick cell line
KROPÁČKOVÁ, Sára
2022 - English
Arthropods, including ticks, are not able to synthetise cholesterol de novo. However, cholesterol is an essential component of their cell membranes and serves as a precursor for the synthesis of steroid hormones. In this thesis, I looked at the biological function of exogenous cholesterol on the tick Ixodes ricinus cell line IRE/CTVM19. I verified the need for cholesterol supplementation in the growth medium for proper cell proliferation. Cellular proliferation was stimulated by cholesterol and not by other sterols (ergosterol, sitosterol). We further explored the two assumed aforementioned cholesterol cellular roles, but also investigated the role of cholesterol as a signal molecule: The effect of cholesterol sensing is described on the tick cell level, as well as on the level of the whole organism, using the ex vivo feeding system of ticks. This work provides novel insights into the cholesterol biology of ticks and tick cells, increasing our understanding of tick-host blood molecules' interaction. Arthropods, including ticks, are not able to synthetise cholesterol de novo. However, cholesterol is an essential component of their cell membranes and serves as a precursor for the synthesis of steroid hormones. In this thesis, I looked at the biological function of exogenous cholesterol on the tick Ixodes ricinus cell line IRE/CTVM19. I verified the need for cholesterol supplementation in the growth medium for proper cell proliferation. Cellular proliferation was stimulated by cholesterol and not by other sterols (ergosterol, sitosterol). We further explored the two assumed aforementioned cholesterol cellular roles, but also investigated the role of cholesterol as a signal molecule: The effect of cholesterol sensing is described on the tick cell level, as well as on the level of the whole organism, using the ex vivo feeding system of ticks. This work provides novel insights into the cholesterol biology of ticks and tick cells, increasing our understanding of tick-host blood molecules' interaction.
Keywords:
tick; cholesterol; tick cell line; IRE/CTVM19; Hedgehog signalling pathway
Available in the Digital Repository of University of South Bohemia.
Subcellular insight into cholesterol-mediated proliferation of the tick cell line
Arthropods, including ticks, are not able to synthetise cholesterol de novo. However, cholesterol is an essential component of their cell membranes and serves as a precursor for the synthesis of ...
Numerical simulations of dynamic processes in the solar corona
BELOV, Sofya
2022 - English
This master's thesis deals with 3D numerical simulations of turbulent flow past magnetic structures in the solar corona, focusing on the phenomenon of vortex shedding. This phenomenon is well known in hydrodynamic conditions. Still, it has not yet been satisfactorily investigated in magnetohydrodynamic (MHD) conditions, such as in the solar atmosphere, where its occurrence has been suggested by several studies and may explain some oscillatory phenomena. Numerical code Lare3d is used for the simulations.
Keywords:
vortex shedding; solar corona; coronal loops; solar prominences; coronal mass ejections; kink oscillations; magnetohydrodynamics; numerical simulations; Lare3d
Available in the Digital Repository of University of South Bohemia.
Numerical simulations of dynamic processes in the solar corona
This master's thesis deals with 3D numerical simulations of turbulent flow past magnetic structures in the solar corona, focusing on the phenomenon of vortex shedding. This phenomenon is well known in ...
The role of Q tRNA modification in codon biased translation in \kur{Leishmania mexicana}
ŠVÁBOVÁ, Monika
2022 - English
The aim of the thesis was to standardize polysome profiling and ribosome profiling protocols for the parasitic protist Leishmania mexicana in order to identify the effect of Q-tRNAs in translation. In addition, we analyzed the role of queuosine (Q) t-RNA modification in the cell cycle of L. mexicana. The proteomics analysis was carried out in order to elucidate the role of Q-tRNAs in protein folding.
Keywords:
Leishmania mexicana; translation; queuosine; modification; ribosome profiling; polysome profiling; tRNA
Available in the Digital Repository of University of South Bohemia.
The role of Q tRNA modification in codon biased translation in \kur{Leishmania mexicana}
The aim of the thesis was to standardize polysome profiling and ribosome profiling protocols for the parasitic protist Leishmania mexicana in order to identify the effect of Q-tRNAs in translation. In ...
Analysis of genetic diversity of \kur{Borrelia burgdorferi} sensu lato and \kur{Borrelia miyamotoi} across Central and Eastern Europe using multilocus sequence typing (MLST) in range of Slovakia and data from the MLST database with focus on Central and Eastern Europe
MORÁVKOVÁ, Veronika
2022 - English
The study was based on tick collection, DNA extraction, PCR amplification, sequencing and computational analyses of Borrelia burgdorferi sensu lato and B. miyamotoi. The study included evaluation on the prevalence of Borrelia burgdorferi sensu lato and B. miyamotoi in Ixodes ricinus ticks across the Slovak Republic based on the collection of ticks from 2017. The spirochetes were further classified into genospecies as well as sequence types (ST) according to MLST method by amplifying and sequencing eight housekeeping genes. Subsequently, population-genetic relationships of Borrelia tick-borne pathogens across Central and Eastern Europe were analysed. The study was based on tick collection, DNA extraction, PCR amplification, sequencing and computational analyses of Borrelia burgdorferi sensu lato and B. miyamotoi. The study included evaluation on the prevalence of Borrelia burgdorferi sensu lato and B. miyamotoi in Ixodes ricinus ticks across the Slovak Republic based on the collection of ticks from 2017. The spirochetes were further classified into genospecies as well as sequence types (ST) according to MLST method by amplifying and sequencing eight housekeeping genes. Subsequently, population-genetic relationships of Borrelia tick-borne pathogens across Central and Eastern Europe were analysed.
Keywords:
Borrelia afzelii; Borrelia garinii; Borrelia burgdorferi sensu stricto; Borrelia miyamotoi; PCR; MLST; MLSA; Ixodes ricinus; sequence type; genetic diversity; Slovak Republic; Slovakia; population-genetic analysis; Lyme borreliosis; Lyme disease; relapsing fever
Available in the Digital Repository of University of South Bohemia.
Analysis of genetic diversity of \kur{Borrelia burgdorferi} sensu lato and \kur{Borrelia miyamotoi} across Central and Eastern Europe using multilocus sequence typing (MLST) in range of Slovakia and data from the MLST database with focus on Central and Eastern Europe
The study was based on tick collection, DNA extraction, PCR amplification, sequencing and computational analyses of Borrelia burgdorferi sensu lato and B. miyamotoi. The study included evaluation on ...
Svalbard reindeer and its interaction with extreme polar environment
KAVANOVÁ, Veronika
2022 - English
Two distinct populations of Svalbard reindeer are compared based on their antler parameters. Relative antler size and number of tines are supposed to reflect well environmental conditions of the sedentary population within the growing season. Both studied populations are well spatially isolated due to high mountain ridges, glaciers and fjords. The population in Petuniabukta occupies sparsely vegetated region with harsh climatic conditions, whereas Skansbukta represents area with continuous tundra vegetation, milder climatic conditions and consequently also longer vegetation season. These environmental factors probably lead to significant differences in relative antler size and number of tines. Skansbukta population has larger relative antler size and higher number of tines than the population in Petuniabukta. Two distinct populations of Svalbard reindeer are compared based on their antler parameters. Relative antler size and number of tines are supposed to reflect well environmental conditions of the sedentary population within the growing season. Both studied populations are well spatially isolated due to high mountain ridges, glaciers and fjords. The population in Petuniabukta occupies sparsely vegetated region with harsh climatic conditions, whereas Skansbukta represents area with continuous tundra vegetation, milder climatic conditions and consequently also longer vegetation season. These environmental factors probably lead to significant differences in relative antler size and number of tines. Skansbukta population has larger relative antler size and higher number of tines than the population in Petuniabukta.
Keywords:
Svalbard reindeer; antlers; population characteristics; environmental conditions
Available in the Digital Repository of University of South Bohemia.
Svalbard reindeer and its interaction with extreme polar environment
Two distinct populations of Svalbard reindeer are compared based on their antler parameters. Relative antler size and number of tines are supposed to reflect well environmental conditions of the ...
Trade-offs in parasitoid resistance and competitive ability in Drosophila
SIDWELL, Jeni
2022 - English
Trade-offs in fitness traits in Drosophila were studied in a laboratory environment. Fly and wasp lines originated from an ecological community in a tropical rainforest in Queensland, Australia and were established in České Budějovice. Experimental evolution was used to impose selective pressures on populations of flies to select for parasitoid resistance and competitive ability. The selection regimes were followed by two separate phenotyping experiments to measure the resulting phenotypic changes and impose novel conditions to the evolved flies in order to identify trade-offs.
Keywords:
ecology; evolution; eco-evolutionary dynamics; trade-offs; fitness; Drosophila
Available in the Digital Repository of University of South Bohemia.
Trade-offs in parasitoid resistance and competitive ability in Drosophila
Trade-offs in fitness traits in Drosophila were studied in a laboratory environment. Fly and wasp lines originated from an ecological community in a tropical rainforest in Queensland, Australia and ...
Differentiating the adhesion properties of surface proteins between various Borrelia species
DANKLMAIER, Anna-Celine
2021 - English
The aim of the thesis was to gain a better understanding of decorin binding proteins A and B (DbpAB) of various Borrelia species my different means. Overexpression of DbpA or DbpB in B. afzelii was addressed, examining phenotypic changes in mutants. Also, it was aimed to create shuttle vectors to complement DbpAB of different European Borrelia species as well as suicide vectors. The aim of the thesis was to gain a better understanding of decorin binding proteins A and B (DbpAB) of various Borrelia species my different means. Overexpression of DbpA or DbpB in B. afzelii was addressed, examining phenotypic changes in mutants. Also, it was aimed to create shuttle vectors to complement DbpAB of different European Borrelia species as well as suicide vectors.
Keywords:
Borrelia; surface proteins; adhesion
Available in the Digital Repository of University of South Bohemia.
Differentiating the adhesion properties of surface proteins between various Borrelia species
The aim of the thesis was to gain a better understanding of decorin binding proteins A and B (DbpAB) of various Borrelia species my different means. Overexpression of DbpA or DbpB in B. afzelii was ...
Design and production of TBEV specific cleavage reporter for in vitro and in cell studies
KOTOUNOVÁ, Eliška
2021 - English
Tick-borne encephalitis virus (TBEV) is an important human pathogen and as of now, there are no specific therapeutics to combat this disease. In vitro and in cell models are essential in studying the disease and in the pursuit of novel therapeutics. This thesis deals with the design and production of two genetically encoded cleavage reporters of TBEV NS2B-NS3 protease activity. The reporters were produced in E. coli and tested with recombinant NS2B-NS3 protease, as well as produced in mammalian cell lines and analyzed for fluorescence upon infection with TBEV. Tick-borne encephalitis virus (TBEV) is an important human pathogen and as of now, there are no specific therapeutics to combat this disease. In vitro and in cell models are essential in studying the disease and in the pursuit of novel therapeutics. This thesis deals with the design and production of two genetically encoded cleavage reporters of TBEV NS2B-NS3 protease activity. The reporters were produced in E. coli and tested with recombinant NS2B-NS3 protease, as well as produced in mammalian cell lines and analyzed for fluorescence upon infection with TBEV.
Keywords:
TBEV; NS2B-NS3 protease; cleavage reporter; PAmCherry
Available in the Digital Repository of University of South Bohemia.
Design and production of TBEV specific cleavage reporter for in vitro and in cell studies
Tick-borne encephalitis virus (TBEV) is an important human pathogen and as of now, there are no specific therapeutics to combat this disease. In vitro and in cell models are essential in studying the ...
The Mitochondrial Contact Site and Cristae Organization System and F1FO-ATP Synthase Crosstalk is a Fundamental Property of Mitochondrial Cristae
CADENA, Lawrence Rudy
2021 - English
The acquisition of mitochondria from an endosymbiont closely related to extant alphaproteobacteria occurred prior to the divergence of modern eukaryotes. Since then, diverse eukaryotes have not only developed a number of different mechanisms to adapt to their environment regarding growth and proliferation, but perpetuated certain traits that have persisted for eons. This thesis postulates an ancestral mechanism for cristae development in mitochondria involving interplay between two cristae shaping protein complexes, the Mitochondrial Contact Site and Cristae Organization System and F1FO-ATP Synthase, that has remained conserved throughout eukaryotic diversification for over 2 billion years. The acquisition of mitochondria from an endosymbiont closely related to extant alphaproteobacteria occurred prior to the divergence of modern eukaryotes. Since then, diverse eukaryotes have not only developed a number of different mechanisms to adapt to their environment regarding growth and proliferation, but perpetuated certain traits that have persisted for eons. This thesis postulates an ancestral mechanism for cristae development in mitochondria involving interplay between two cristae shaping protein complexes, the Mitochondrial Contact Site and Cristae Organization System and F1FO-ATP Synthase, that has remained conserved throughout eukaryotic diversification for over 2 billion years.
Keywords:
Mitochondria; MICOS; ATP Synthase; Trypanosoma brucei; evolution; protein interaction
Available in the Digital Repository of University of South Bohemia.
The Mitochondrial Contact Site and Cristae Organization System and F1FO-ATP Synthase Crosstalk is a Fundamental Property of Mitochondrial Cristae
The acquisition of mitochondria from an endosymbiont closely related to extant alphaproteobacteria occurred prior to the divergence of modern eukaryotes. Since then, diverse eukaryotes have not only ...
Teacher questions supporting communication in the classroom (on the material of ELT)
BRONOVÁ, Pavlína
2021 - English
Diplomová práce se zabývá otázkami učitele ve třídních interakcích ve výuce anglického jazyka na 2. stupni ZŠ. Mnoho výzkumů totiž ukazuje, že kvalitní otázky učitele vedou k rozvoji komunikace u žáků v cizojazyčné výuce, zvyšují kvalitu a množství žákova jazyka. Teoretická část práce nejprve představuje postavení angličtiny jako školního předmětu v Rámcovém vzdělávacím programu. Nejrozsáhlejší část práce se zabývá vymezením pojmů třídní komunikace a interakce mezi učitelem a žákem nazývanou v anglicky psané literatuře jako IRE nebo IRF. Práce se zaměřuje na iniciaci ve formě otázky a na základě odborné literatury popisuje typy otázek podle různých typologií a zabývá se tím, které otázky učitele jsou ve třídní interakci nejefektivnější. Praktická část vychází z videonahrávek hodin anglického jazyka na 2. stupni ZŠ. Otázky analyzuje na základě popsané typologie a sleduje, které typy otázek nejvíce rozvíjejí komunikaci ve třídě a které ji naopak limitují. The diploma thesis deals with the teacher questions in classroom interaction in the teaching of English at the second stage of primary school. Many studies show that quality teacher questions develop pupils' communication in foreign language teaching, increase the quality and quantity of the pupil's language. The theoretical part of the thesis first presents the position of English as a school subject within the Framework Educational Programme. The most extensive part of the thesis deals with the terms of classroom communication and interaction known as IRE or IRF. Based on the literature, the thesis focuses on initiation in the form of a question and describes the types of questions according to different typologies and tries to find out which teacher questions are most effective in classroom interaction. The practical part is based on video recordings of English language lessons at the second stage of primary school. This part analyses the questions based on the described typology and observes which types of teacher questions develop classroom communication and which, on the contrary, limit it.
Keywords:
Otázky učitele; pedagogická komunikace; pedagogická interakce; IRF struktura; dialog
Available in the Digital Repository of University of South Bohemia.
Teacher questions supporting communication in the classroom (on the material of ELT)
Diplomová práce se zabývá otázkami učitele ve třídních interakcích ve výuce anglického jazyka na 2. stupni ZŠ. Mnoho výzkumů totiž ukazuje, že kvalitní otázky učitele vedou k rozvoji komunikace u žáků ...
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