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Charakterizace organických sloučenin v PM1 aerosolu v Kladně v zimním období 2016
Křůmal, Kamil; Mikuška, Pavel; Večeřa, Zbyněk
2016 - Czech
Organické sloučeniny a markery používané pro identifikaci hlavních emisních zdrojů byly měřeny v PM1 aerosolu v malém městě Kladně-Švermově. Nejvyšší koncentrace byly pro anhydridy monosacharidů a pryskyřičné kyseliny (spalování biomasy). Dalšími analyzovanými skupinami organických sloučenin a markerů byly polycyklické aromatické uhlovodíky (emise z dopravy a nedokonalého spalování), hopany (spalování uhlí, doprava), sterany (doprava), sacharidy a alkany. In this work the organic compounds and organic markers used for the identification of main aerosol sources were measured in PM1 aerosol in a small town (Kladno-Švermov) near Prague. Monosaccharide anhydrides and resin acids (emissions from biomass combustion) were found especially. Polycyclic aromatic hydrocarbons (emissions from traffic and incomplete combustion), hopanes (traffic, coal combustion), steranes (traffic), saccharides and alkanes were other groups of analysed organic compounds.
Keywords:
organic tracer; urban aerosol; PM1
Available in digital repository of the ASCR
Charakterizace organických sloučenin v PM1 aerosolu v Kladně v zimním období 2016
Organické sloučeniny a markery používané pro identifikaci hlavních emisních zdrojů byly měřeny v PM1 aerosolu v malém městě Kladně-Švermově. Nejvyšší koncentrace byly pro anhydridy monosacharidů a ...
A novel nanospray liquid junction interface for versatile CE-MS
Křenková, Jana; Haselberg, R.; Somsen, G. W.; Foret, František
2016 - English
In this work we present a new polyimide-based liquid junction nanospray interface, combining the advantages of sheath flow and sheathless designs. The liquid junction interface was prepared as a hybrid microfabricated plastic liquid junction sprayer allowing to perform analysis in standard (30-100 micro m ID) separation capillaries. The interface incorporates a self-aligning port for coupling of the separation capillary as well as ports for automated flushing of both the liquid junction and the separation capillary. The interface permits performing CE both with and without electroosmotic flow. The use of large bore capillaries allows injection and preconcentration of larger sample volumes, favoring detection sensitivity. Different compositions of the spray liquid and BGE can be used when needed.
Keywords:
capillary electrophoresis; mass spectrometry; liquid junction interface
Available in digital repository of the ASCR
A novel nanospray liquid junction interface for versatile CE-MS
In this work we present a new polyimide-based liquid junction nanospray interface, combining the advantages of sheath flow and sheathless designs. The liquid junction interface was prepared as a ...
Determination of levoglucosan in urban aerosol
Coufalík, Pavel; Mikuška, Pavel; Čmelík, Richard
2016 - English
The main aim of this work was a development of the analytical method for levoglucosan\ndetermination including direct collection of aerosol into water phase, preconcentration,\nand determination by LC-MS.
Keywords:
monosaccharide anhydrides; urban aerosol; levoglucosan
Available in digital repository of the ASCR
Determination of levoglucosan in urban aerosol
The main aim of this work was a development of the analytical method for levoglucosan\ndetermination including direct collection of aerosol into water phase, preconcentration,\nand determination by ...
Zvýšení účinnosti kontinuálního aerosolového vzorkovače
Mikuška, Pavel; Čapka, Lukáš; Večeřa, Zbyněk
2016 - Czech
Příspěvek se zabývá zvýšením účinnosti záchytu ACTJU vzorkovače i pro částice menší než 300 nm. Použitím tzv. „růstové trubky“ (RT), která je umístěna před vzorkovačem, vzroste velikost částic na několik µm a zvětšené částice jsou pak snadno kvantitativně zachyceny v ACTJU vzorkovači. Contribution deals with increasing of collection efficiency of ACTJU sampler also for particles smaller than 300 nm in diameter. Use of so-called „growth tube“ (GT) located upstream of the ACTJU results in increase of particle size to several µm. Enlarged particles are then easily quantitative collected in ACTJU sampler.
Keywords:
continuous sampler; on-line analysis; atmospheric aerosol
Available in digital repository of the ASCR
Zvýšení účinnosti kontinuálního aerosolového vzorkovače
Příspěvek se zabývá zvýšením účinnosti záchytu ACTJU vzorkovače i pro částice menší než 300 nm. Použitím tzv. „růstové trubky“ (RT), která je umístěna před vzorkovačem, vzroste velikost částic na ...
Effect of two retinoic acid isomers on the expression of selected nuclear retinoid receptors and protein pattern in human MCF-7 breast cancer cell line
Brtko, J.; Toporová, L.; Macejová, D.; Bialešová, L.; Flodrová, Dana; Bobálová, Janette
2016 - English
Effect of two retinoic acid isomers on RARalpha, RARgamma, RXRalpha, RXRbeta expression and protein profile in human MCF-7 breast cancer line were investigated.
Keywords:
retinoic acid; retinoid receptors; protein pattern
Available in digital repository of the ASCR
Effect of two retinoic acid isomers on the expression of selected nuclear retinoid receptors and protein pattern in human MCF-7 breast cancer cell line
Effect of two retinoic acid isomers on RARalpha, RARgamma, RXRalpha, RXRbeta expression and protein profile in human MCF-7 breast cancer line were investigated.
Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
Ledvina, Vojtěch; Klepárník, Karel
2016 - English
Caspases are proteases that play key role in the process of apoptosis, the programmed\ncell death. Among them, caspase-3 and -7 are main executioner caspases that cleave\nmany vital proteins during apoptosis and after their widespread activation, the process\ncannot be reversed. To analyze caspase-3/7 activation within single cells, a miniaturized\ndevice for parallel analysis of eight samples was developed. The assay is based on the\nmodified luciferin-firefly luciferase bioluminescence (BL) system. Individual\nsuspended cells were collected and transferred into detection microvials using a\nmicromanipulator. The bioluminescence was detected using a photon counting head\nwith cooled photcathode. The LOD suitable for detection of active caspase-3/7 in both\napoptotic and non-apoptotic cells was reached.
Keywords:
single-cell analysis; apoptotic cells; non-apoptotic cells
Available in digital repository of the ASCR
Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
Caspases are proteases that play key role in the process of apoptosis, the programmed\ncell death. Among them, caspase-3 and -7 are main executioner caspases that cleave\nmany vital proteins during ...
Novel, versatile capillary electrophoresis instrument with laser induced fluorescence for analysis of various lipid peroxidation biomarkers
Lačná, J.; Foret, František; Kubáň, Petr
2016 - English
In this work we have developed a new sensitive method for quantitation of MDA in\nbiological samples by CE-LIF with LOD about 1.7 nM. First part of the work was\ndevoted to the construction of a new CE-LIF system. Then the derivatization process\nof MDA by TBA was optimized. The ability to use this method for the detection of\nMDA in a biological samples, such as EBC and plasma, was also demonstrated. For the\nfirst time it was possible to detect MDA in EBC samples by CE. Other biomarkers are\nnow being studied.
Keywords:
CE-LIF; malondialdehyde; biomarkers; lipids
Available in digital repository of the ASCR
Novel, versatile capillary electrophoresis instrument with laser induced fluorescence for analysis of various lipid peroxidation biomarkers
In this work we have developed a new sensitive method for quantitation of MDA in\nbiological samples by CE-LIF with LOD about 1.7 nM. First part of the work was\ndevoted to the construction of a new ...
Micro-electromembrane extraction across multiple aqueous and organic phases for selective pretreatment of raw biological samples
Kubáň, Pavel; Boček, Petr; Seip, K. F.; Gjelstad, A.; Pedersen-Bjergaard, S.
2016 - English
Micro-electromembrane extraction (μ-EME) across multiple aqueous and organic\nsolutions was developed for selective extractions of various analytes from untreated\nbiological samples. The novel approach requires μL volumes of samples and organic\nsolvents, which form consecutive immiscible plugs in a transparent polymeric capillary\nand the extraction process is accelerated by application of d.c. voltage. Number of the\nplugs, their composition, volume and sequence in the extraction capillary can be chosen\nspecifically for each particular application and high variability and selectivity of the\nextraction process can be achieved. In this contribution, we demonstrate suitability of\na five-phase μ-EME system for simultaneous extractions of cations and anions from\nurine and for selective extractions of basic analytes based on their acid-base strength\nfrom plasma.
Keywords:
micro-electromembrane extraction; sample pretreatment techniques; biological samples
Available in digital repository of the ASCR
Micro-electromembrane extraction across multiple aqueous and organic phases for selective pretreatment of raw biological samples
Micro-electromembrane extraction (μ-EME) across multiple aqueous and organic\nsolutions was developed for selective extractions of various analytes from untreated\nbiological samples. The novel ...
Characterization of FRET sensor
Datinská, Vladimíra; Klepárník, Karel; Belšánová, B.; Minárik, M.; Foret, František
2016 - English
In this study, we present characterization of sensor based on Fӧrster resonance energy\ntransfer (FRET). The sensor is composed of ssDNA chain attached to a laboratory\nsynthesized quantum dot (QD). A complementary chain of a sample is labeled by a\nluminescent dye. When the dsDNA hybrid is formed, the energy from the QD (donor)\nis transferred to the dye (acceptor) and FRET is observed as a decrease of QD\nluminescence emission intensity and an increase of dye luminescence emission\nintensity.
Keywords:
FRET sensor; Fӧrster resonance energy transfer; quantum dot
Available in digital repository of the ASCR
Characterization of FRET sensor
In this study, we present characterization of sensor based on Fӧrster resonance energy\ntransfer (FRET). The sensor is composed of ssDNA chain attached to a laboratory\nsynthesized quantum dot (QD). A ...
Microfluidic device for cell counting and characterization
Václavek, Tomáš; Křenková, Jana; Foret, František
2016 - English
Integration of cell counting unit is an important step in developing of microfluidic\ndevice for cellular analysis. Here we present a microfluidic device for cell counting\nbased on resistive pulse sensing.
Keywords:
microfluidic devices; microfluidic chip; cellular analysis
Available in digital repository of the ASCR
Microfluidic device for cell counting and characterization
Integration of cell counting unit is an important step in developing of microfluidic\ndevice for cellular analysis. Here we present a microfluidic device for cell counting\nbased on resistive pulse ...
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