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Estimation of the post-meiotic state of spermatogenesis in man by immunofluorescence with monoclonal antibodies against intra-acrosomal sperm proteins
Chládek, David; Pěknicová, Jana
2003 - English
Male infertility due to severe azoospermia has been associated with the absence of spermatozoa in ejaculated. We tested the ejaculates (IVF latoratory Pranatal Ltd) from 30 patients with azoospermia for the presence of cells from defferent stages of spermiogenesis. Monoclonal sntibodews against intraacrosomal human sperm proteins were used in the immunofluorescence test for detection of acrosomal proteins in the acrosomal precursors and acrosomes.
Keywords:
monoclonal antibody; acrosome
Available at various institutes of the ASCR
Estimation of the post-meiotic state of spermatogenesis in man by immunofluorescence with monoclonal antibodies against intra-acrosomal sperm proteins
Male infertility due to severe azoospermia has been associated with the absence of spermatozoa in ejaculated. We tested the ejaculates (IVF latoratory Pranatal Ltd) from 30 patients with azoospermia ...
Dynamic and role of cytosekeletal proteins in mammalian sperm cells
Paleček, J.; Dvořáková, K.; Pěknicová, Jana; Kubátová, Alena; Dráber, Pavel
2003 - English
The cytoskeleton of sperm head consists of resistant structural proteins of the nucleus and of perinuclear theca, which are largely rosponsible for the shaping of the nucleus. three mahjor cyteskeletal proteins, actin, tzubnulin and spectrin, are present in the head of mammalian spermatohzoa. Maoreober, the mictotubular cytoskeleton also contains other dynamic proteins, which have a morphological function within the cell and which are responsible for cell signalling processes.
Keywords:
cyprinus; carpio; acipenser
Available at various institutes of the ASCR
Dynamic and role of cytosekeletal proteins in mammalian sperm cells
The cytoskeleton of sperm head consists of resistant structural proteins of the nucleus and of perinuclear theca, which are largely rosponsible for the shaping of the nucleus. three mahjor ...
Regulace protizánětlivých imunitních reakcí v předním segmentu oka
Vítová, A.; Filipec, M.; Pindjáková, Jana; Krulová, Magdalena; Zajícová, Alena; Holáň, Vladimír
2003 - Czech
Rohovkové stroma bylo identifikováno jako zdroj nové molekuly zodpovědné za selektivní inhibici produkce protizánětlivých cytokinů. Corneal stroma was identified as a source of a novel molecule rosponsible for selective ingibitor of production of anti-inflammatory cytokines.
Keywords:
Rohovkové stroma; selektivní inhibice
Available at various institutes of the ASCR
Regulace protizánětlivých imunitních reakcí v předním segmentu oka
Rohovkové stroma bylo identifikováno jako zdroj nové molekuly zodpovědné za selektivní inhibici produkce protizánětlivých cytokinů....
Molecular cloning, E.coli expression and purification of SCFV antibody fragments of diagnostic/therapeutic interest
Král, Vlastimil; Fábry, Milan; Hořejší, Magdalena; Závada, Jan; Sedláček, Juraj
2003 - English
We describe molecular cloning, expression, purification and properties of two single chain antibody variable fragments (scFv) of potential diagnostic use, namely scFv M75 and scFv Tu-20. The former scFv is derived from a monoclonal antibody M75 specific for a cell surface protein MN/CA IX, strongly associated with many types of human carcinomas. The latter scFv is derived from a monoclonal antibody TU-20 specific for neuronal beta-III-tubulin.
Keywords:
cloning; purification; scFv
Available at various institutes of the ASCR
Molecular cloning, E.coli expression and purification of SCFV antibody fragments of diagnostic/therapeutic interest
We describe molecular cloning, expression, purification and properties of two single chain antibody variable fragments (scFv) of potential diagnostic use, namely scFv M75 and scFv Tu-20. The former ...
Antitrophoblast cell-mediated immunity /AT-CMI/ in recurrent miscarriage and ectopic pregnacy
Madar, J.; Kučera, E.; Šůla, K.; Chaloupková, A.; Pěknicová, Jana; Tolarová, M.; Nouza, K.; Kinský, R.
2003 - English
Specific mechanisms operate in decidua nad trophoblast to establish and maintain immunological tolerance of the embryo in mother's uterus. due to the lack of this tolerance, disturbances of pregnancy may result. In this presentation, we have tested the hypothesis that anti-trohhoblast cell-mediated immunity (AT-CMI) is elevated in habitually aborting women and that extra-uterine localisation of the embryo leads to subsequent elevation of AT-CMI.
Keywords:
trophoblast; cardiolipin
Available at various institutes of the ASCR
Antitrophoblast cell-mediated immunity /AT-CMI/ in recurrent miscarriage and ectopic pregnacy
Specific mechanisms operate in decidua nad trophoblast to establish and maintain immunological tolerance of the embryo in mother's uterus. due to the lack of this tolerance, disturbances of pregnancy ...
Multigenerational study - effect of the selected compounds on the mouse fertility
Boubelík, Michael; Pěknicová, Jana; Kyselová, Vendula; Buckiová, D.
2003 - English
Several compounds known to be effective for their influence onto the environment were tested. Compounds tested in this study were: benzo/a/ pyrene, lindane (hexachlorocyclohexane isomer), 4-n-nonylphenol, bisphenol A and octachlorostyrene. The question was if these compounds might also function as endocrine disruptors.
Keywords:
endocrine disruptors; sperm motility
Available at various institutes of the ASCR
Multigenerational study - effect of the selected compounds on the mouse fertility
Several compounds known to be effective for their influence onto the environment were tested. Compounds tested in this study were: benzo/a/ pyrene, lindane (hexachlorocyclohexane isomer), ...
Microsatellite region in the murine oncogene src - its primary structure and variability during evolution
Fučík, Vladimír; Šindelka, Radek; Beran, Jaroslav; Ferjentsik, Zoltán; Jonák, Jiří
2003 - English
In the fifth intron of the Mus musculus oncogene src a complex microsatellite was discovered that consists of a CA run and 31 bp apart followed by track of TG dinucleotides interrupted by extra Gs in specific intervals. As the 31 bp interlink can assume a hairpin structure, the whole region can be viewed as an imperfect inverted repeat. The microsatellite was sequenced in imbred lines of M. m. musculus and M. m. domesticus, as well as in mice of both subspecies trapped near the hybrid zone. Differences in the length of repeat regions, insertion/deletions and simple base changes characterize the strains. Hybrids between the two species could be identified by sequencing of cloned PCR products. The structure of the microsatellite in more distant species was found to be conspicuously different. Instead of frequent (CA)18-25, we can see only (CA)8 in M. spretus, CATGCGCCCCCCA in Mus pahari and CACCCCCC in Mus caroli. No similar structure is present in the corresponding human src intron.
Keywords:
Mus musculus, c-src introns, microsatellite
Available at various institutes of the ASCR
Microsatellite region in the murine oncogene src - its primary structure and variability during evolution
In the fifth intron of the Mus musculus oncogene src a complex microsatellite was discovered that consists of a CA run and 31 bp apart followed by track of TG dinucleotides interrupted by extra Gs in ...
A Phenylnorstatine Inhibitor Binding to HIV-1 Protease: Geometry, Protonation and Subsite-Pocket Interactions Analyzed at Atomic Resolution
Brynda, Jiří; Řezáčová, Pavlína; Fábry, Milan; Hořejší, Magdalena; Štouračová, Renata; Sedláček, Juraj; Souček, M.; Hradílek, M.; Lepšík, M.; Konvalinka, J.
2003 - English
The x-ray structure of a complex of HIV-1 protease (PR) with a phenylnorstatine inhibitor Z-Pns-Phe-Glu-Glu-NH2 has been determined at 1.03 A, the highest resolution so far reported for any HIV PR complex. The inhibiot shows subnanomolar Ki values for both the wild-type PR and the variant representing one of the most common mutations linked to resistance developoment. The structure displays a unique pattern of hydrogen bonding to the two catalytic aspartate residues. The high resolution permits to assess the donor/acceptor realtions of this hydrogen bonding and to indicate a proton shared by the two catalytic residues. Structural mechanism for the unimpaired ihnibition of the protease Val82Ala mutant is also suggested, based on energy calculations and analyses.
Keywords:
inhibitor; HIV protease; atomic resolution
Available at various institutes of the ASCR
A Phenylnorstatine Inhibitor Binding to HIV-1 Protease: Geometry, Protonation and Subsite-Pocket Interactions Analyzed at Atomic Resolution
The x-ray structure of a complex of HIV-1 protease (PR) with a phenylnorstatine inhibitor Z-Pns-Phe-Glu-Glu-NH2 has been determined at 1.03 A, the highest resolution so far reported for any HIV PR ...
Metody experimentálního určení fází (MIR, MAD,SAD) v krystalografické analýze biologických makromolekul
Brynda, Jiří
2003 - Czech
Důležitost metod poskytujících nepředpojaté řešení fázového problému se stává vstupem molekulární biologie a biochemie do tzv. post-genomické éry stále významnější. V článku jsou popsány klasické metody experimentálního určení fází:1) Metoda isomorfních derivátů s těžkými atomy 2) Metoda Anomálního rozptylu při různých vlnových délkách 3) Metoda Anomálního rozptylu při jedné vlnové délce Čím lepší jsou vaše první, experimentální mapy, tím více modelu můžete spolehlivě vystavět do nepředpojaté (modelem nezatížené) mapy před sečtením fází (původní + model). MAD fáze pocházejí z perfektně izomorfních dat a tak netrpí disordrem jako je to běžné u MIR map na malém rozlišení. MIR fáze zase na druhou stranu poskytují výtečnou fázovací sílu na nízkém rozlišení a výsledkem je lepší propojení mapy v prostoru. Není proto překvapivé, že nejlepší mapy jsou buď MAD mapy nebo kombinované MIR-MAD mapy. Importance of unbiased phases in post genomic era is emphasized. Article describes the classical methods of phase determination: 1) Multiple Isomorphous replacement, heavy-atom derivative (MIR) 2) Multiple Anomalous Dispersion (MAD) Phasing 3) Single Anomalous Dispersion (SAD) Phasing The better your initial, experimental map is, the more model you will be able to build confidently into this unbiased map before proceeding to phase combination. MAD phases result from perfectly isomorphous data, and do not deteriorate at low resolution as do MIR phases. MIR phases, on the other hand, provide superior phasing power at low resolution resulting in better connectivity. Not surprising, the best maps are either MAD or combined MIR-MAD maps.
Keywords:
phase problem; MIR,MAD,SAD
Available at various institutes of the ASCR
Metody experimentálního určení fází (MIR, MAD,SAD) v krystalografické analýze biologických makromolekul
Důležitost metod poskytujících nepředpojaté řešení fázového problému se stává vstupem molekulární biologie a biochemie do tzv. post-genomické éry stále významnější. V článku jsou popsány klasické ...
Crystallographic study of an anti=carbonic anhydrase IX monoclonal antibody M75
Štouračová, Renata; Závada, Jan; Závadová, Zuzana; Pastoreková, S.; Brynda, Jiří; Fábry, Milan; Král, Vlastimil; Hořejší, Magdalena; Sedláček, Juraj
2003 - English
Carbonic anhydrase IX (CA IX) is a cell surface protein, strongly associated with certain types of human carcinomas. Structural study of a CA IX-binding monoclonal antibody (mAb) M75, complexed with its epitope peptide may contribute toward elucidation of the role of CA IX. Monoclonal antibody M75 was obtained and proved to react excellently with native and denaturated CA IX. Using synthetic oligopeptides, the epitope of mAb M75 was localized in the proteoglycan domain of CA IX, in the region of a tandem repeat and identified as amino acids PGEEDLP. The Fab fragment was obtained by papain cleavage. We obtained crystals of free Fab M75 and Fab M75 complexed with two different epitope peptides. The data set for Fab M75 was collected and the structure solving is underway.
Keywords:
anti-carbonic ahydrase antibody, cancer, crystallization
Available at various institutes of the ASCR
Crystallographic study of an anti=carbonic anhydrase IX monoclonal antibody M75
Carbonic anhydrase IX (CA IX) is a cell surface protein, strongly associated with certain types of human carcinomas. Structural study of a CA IX-binding monoclonal antibody (mAb) M75, complexed with ...
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