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TEMPERATURE INFLUENCE ON DESORPTION AND DETERMINATION OF BIOACCESSIBLE PAHS
Čvančarová, Monika; Pokorná, Lenka; Velebová, R.; Najmanová, P.; Cajthaml, Tomáš
2013 - English
Hydrophobic organic pollutants tend to adsorb onto soil particles which can cause a significant decrease in their bioaccessible fractions. Definition of the bioaccessibility is currently not unified; however it represents a part of total PAHs amount which is potentially available for biological processes. It is an important parameter which can be used for prediction of microbial degradation and environmental risk evaluation. This paper studies desorption behavior of PAHs at 20 and 60˚C. The desorption of PAHs from long-term contaminated soils was evaluated using resin Amberlite XAD- 2. A two-phase desorption model was used to calculate the desorption kinetic curves and F fractions which represent bioaccessible amount of PAHs. The results showed that the desorption of PAHs was significantly influenced by temperature. The greatest differences in the desorption behavior were observed at 5–6 ring PAHs. We compared the F fractions and the rate constants of individual PAHs which were determined at 20 and 60˚C. The results were also compared with sequential supercritical fluid extraction which has been already used for prediction of microbial degradation.
Keywords:
pahs
Available at various institutes of the ASCR
TEMPERATURE INFLUENCE ON DESORPTION AND DETERMINATION OF BIOACCESSIBLE PAHS
Hydrophobic organic pollutants tend to adsorb onto soil particles which can cause a significant decrease in their bioaccessible fractions. Definition of the bioaccessibility is currently not unified; ...
ENDOCRINE DISUPTIVE CHEMICALS: MECHANISMS OF WHITE ROT FUNGI BIODEGRADATION
Cajthaml, Tomáš; Křesinová, Zdena; Čvančarová, Monika; Maternová, H.; Filipová, A.
2013 - English
The main aim of this study was to elucidate mechanisms involved in EE2 and other EDs compounds degradation by white rot fungi. Results showed simultaneous involvement of ligninolytic enzymes and cytochrome P-450 in biodegradation. The present results indicate the advantage of utilization of WRF cultures for organopollutants degradation.
Keywords:
biodegradation
Available at various institutes of the ASCR
ENDOCRINE DISUPTIVE CHEMICALS: MECHANISMS OF WHITE ROT FUNGI BIODEGRADATION
The main aim of this study was to elucidate mechanisms involved in EE2 and other EDs compounds degradation by white rot fungi. Results showed simultaneous involvement of ligninolytic enzymes and ...
A NOVEL BIOAUGMENTATION APPROACH IN BIODEGRADATION OF ORGANIC POLLUTANTS IN CONTAMINATED SOIL
Lhotský, O.; Filipová, Alena; Innemanová, P.; Velebová, R.; Cajthaml, Tomáš
2013 - English
Polycyclic aromatic hydrocarbons (PAHs) belong among organic compounds that accumulate in the environment mainly resulting from human activities. Because of their potentially negative effects on human health, methods of their removal from the environment are intensively studied. In this work, a biodegradation method of bioaugmentation based on sequential stepwise mixing of inoculated soil with non inoculated soil was used. The subject of this work was an application of a bacterial consortium on a real contaminated (7399 mg.kg-1 of PAHs) soil. 8 bacterial strains isolated from other contaminated sites were inoculated. DNA was isolated from the samples during the inoculation process and the degradation of PAHs was monitored after 4 months.
Keywords:
soil
Available at various institutes of the ASCR
A NOVEL BIOAUGMENTATION APPROACH IN BIODEGRADATION OF ORGANIC POLLUTANTS IN CONTAMINATED SOIL
Polycyclic aromatic hydrocarbons (PAHs) belong among organic compounds that accumulate in the environment mainly resulting from human activities. Because of their potentially negative effects on human ...
Microalgae Chlorella sp. as an Alternative Source of Fermentable Sugars
Maršálková, B.; Širmerová, M.; Kuřec, M.; Brányik, T.; Brányiková, Irena; Melzoch, K.; Zachleder, Vilém
2010 - English
The aim of this work was to optimize the enzymatic hydrolysis of starch originating from microalgae (34.0 +/- 1.2% wt. starch in the dry algal biomass) grown on flue gas from waste incineration plant and to study the influence cell disintegration method on the yield of hydrolysis. Apart from the starch, the possibility of cellulose usage from microalgae cell wall as a further source of fermentable sugars was studied and preliminary results are presented.
Keywords:
Flue-gas; Biofuels; Photobioredactor
Available at various institutes of the ASCR
Microalgae Chlorella sp. as an Alternative Source of Fermentable Sugars
The aim of this work was to optimize the enzymatic hydrolysis of starch originating from microalgae (34.0 +/- 1.2% wt. starch in the dry algal biomass) grown on flue gas from waste incineration plant ...
Imunomodulační efekt bakteriálního lyzátu Lactobacillus Casei DN 1140001 na vznik a vývoj experimentálně vyvolaného střevního zánětu
Zákostelská, Zuzana; Kverka, Miloslav; Rossmann, Pavel; Klimešová, Klára; Mrázek, Jakub; Kopečný, Jan; Tlaskalová-Hogenová, Helena
2010 - Czech
Lactobacillus casei DN 114001 patří mezi jedno z nejvíce studovaných probiotik současnosti. Cílem této práce bylo zjistit, zda frakce izolované z tohoto probiotika dokáží zmírnit průběh střevního zánětu. Lactobacillus casei DN 114 001 belongs to one of the most studied probiotics today. The aim of this study was to determine whether this fraction isolated from probiotics can alleviate the course of intestinal inflammation
Keywords:
probiotics
Available at various institutes of the ASCR
Imunomodulační efekt bakteriálního lyzátu Lactobacillus Casei DN 1140001 na vznik a vývoj experimentálně vyvolaného střevního zánětu
Lactobacillus casei DN 114001 patří mezi jedno z nejvíce studovaných probiotik současnosti. Cílem této práce bylo zjistit, zda frakce izolované z tohoto probiotika dokáží zmírnit průběh střevního ...
Litter decomposition in a primary succession chronosequence on brown coal mine deposits
Urbanová, Michaela; Valášková, Vendula; Šnajdr, Jaroslav; Baldrian, Petr
2009 - English
Litter decomposition in a primary succession chronosequence on brown coal mine deposits varies along with the successional age of the site
Keywords:
soil; enzyme; succesion
Available at various institutes of the ASCR
Litter decomposition in a primary succession chronosequence on brown coal mine deposits
Litter decomposition in a primary succession chronosequence on brown coal mine deposits varies along with the successional age of the site
Fungal community and enzyme activities in topsoil layers of Quercus petraea forest: how important is the seasonality?
Voříšková, Jana; Valášková, Vendula; Baldrian, Petr
2009 - English
The analysis of fungal community composition and enzyme activities in topsoil layers of a Quercus petraea forest show that seasonality is an important factor affecting community composition
Keywords:
soil; enzyme; litter
Available at various institutes of the ASCR
Fungal community and enzyme activities in topsoil layers of Quercus petraea forest: how important is the seasonality?
The analysis of fungal community composition and enzyme activities in topsoil layers of a Quercus petraea forest show that seasonality is an important factor affecting community composition
Production of Enzymes by Fungal Colonies in Soils: A Laboratory Study
Šnajdr, Jaroslav; Popelářová, Petra; Baadri, S.; Alawi, A.; Boddy, L.; Baldrian, Petr
2009 - English
Production of enzymes by fungal colonies in soils is affected by mycelium age and the presence or absence of interspecific mycelial interactions
Keywords:
soil; enzyme; interaction
Available at various institutes of the ASCR
Production of Enzymes by Fungal Colonies in Soils: A Laboratory Study
Production of enzymes by fungal colonies in soils is affected by mycelium age and the presence or absence of interspecific mycelial interactions
Mikrobiální biodegradace 17alfa-ethinylestradiolu a bisfenolu A
Křesinová, Zdena; Svobodová, Kateřina; Moeder, M.; Cajthaml, Tomáš
2009 - Czech
17 ethinylestradiol (EE2) a bisfenol A (BA) patří mezi skupinu látek označovaných jako endokrinní disruptory (ED). Tyto látky mají negativní vliv na hormonální systém živočichů a člověka, a představují závažný problém z hlediska kontaminace životního prostředí. Tato práce byla zaměřená na biodegradace uvedených látek pomocí osmi druhů hub bílé hniloby v tekutých médiích. Již během prvních tří dnů kultivace se zástupci kmenů Pleurotus ostreatus, Pycnoporus cinnabarinus a Irpex lacteus poklesla koncentrace obou ED z původní koncentrace10 ppm pod limit detekce. Naopak v experimentu s Phanerochaete chrysosporium nebyl zaznamenán pokles koncentrace EE2 a BA ani po dvou týdnech kultivace. Během degradačního pokusu byl dále měřen vliv přítomnosti EE2 a BA na expresi ligninolytických enzymů hub bílé hniloby (lakasa, manganperoxidasa a ligninperoxidasa), které by mohly participovat na biodegradačních procesech 17ethinylestradiol (EE2) and bisphenol A (BA) are endocrine disrupting compounds (ED). ED have a negative influence on animals and human hormonal systems animals, and thus they are serious envronmental contaminants. This work was focused on biodegradtion of EE2 and BA using 8 white rot fungi strains in liquid media. Pleurotus ostreatus, Pycnoporus cinnabarinus and Irpex lacteus were able to remove both ED under detection limit (initial concetration was 10 ppm) during the first three days of cultivation. In contrary, Phanerochaete chrysosporium was not able to degrade both ED durin 2 weaks long cultivation
Keywords:
ligninolytic fungi; biobiodegradation; endocrine disrupting compounds
Available at various institutes of the ASCR
Mikrobiální biodegradace 17alfa-ethinylestradiolu a bisfenolu A
17 ethinylestradiol (EE2) a bisfenol A (BA) patří mezi skupinu látek označovaných jako endokrinní disruptory (ED). Tyto látky mají negativní vliv na hormonální systém živočichů a člověka, a ...
Charakterizace mikrobiálních populací v průběhu kompostování kontaminované půdy
Petránková, Mirka; Čechová, Zuzana; Brennerová, Mária; Cajthaml, Tomáš
2009 - Czech
Bioremediace je jedna z nejvýhodnějších metod pro dekontaminaci znečištěných půd. Její účinnost závisí na několika faktorech, nejvíce však na schopnosti bioremediačních organismů degradovat cílové polutanty, na fyzikálně-chemických vlastnostech polutantů (např. rozpustnost ve vodě) a na vlastnostech půdy. Kompostování se ukazuje jako vhodná technika pro remediaci půd kontaminovaných např. PAU. Proces je založen na schopnosti mikrorganismů degradovat organický materiál v závislosti na termogenezi. Teplota uvnitř kompostovací jednotky může dosáhnout až 75 stupňů C. Pro remediaci je kompostovací substrát jednoduše smíchán s částí kontaminované půdy Bioremediation is an indisputably advantageous approach to cleaning up contaminated soils. The efficiency of bioremediation depends on a number of factors, the most important of which are the ability of the bioremediative organism to degrade the target pollutants, the pollutant’s intrinsic properties (aqueous solubility being of key importance), and soil parameters. Composting has been recently shown to be efficient for remediation of soils contaminated e.g. with PAHs
Keywords:
Phospholipid fatty acids; biodegradation; composting
Available at various institutes of the ASCR
Charakterizace mikrobiálních populací v průběhu kompostování kontaminované půdy
Bioremediace je jedna z nejvýhodnějších metod pro dekontaminaci znečištěných půd. Její účinnost závisí na několika faktorech, nejvíce však na schopnosti bioremediačních organismů degradovat cílové ...
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