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Affinity microfluidic chip based on packed microbeads
Novotný, Jakub; Ostatná, Veronika; Foret, František
2018 - English
In this project, a plastic device was fabricated using CNC milling in polycarbonate. Chip contains integrated pneumatic valves for flow control and allows for injection and stacking of beaded supports. Intended use of these supports is formation of packed affinity columns suitable for glycoprotein assays.
Keywords:
microfluidic chips; microfluidic systems; microfluidic devices
Fulltext is available at external website.
Affinity microfluidic chip based on packed microbeads
In this project, a plastic device was fabricated using CNC milling in polycarbonate. Chip contains integrated pneumatic valves for flow control and allows for injection and stacking of beaded ...
Hexahistidine-based tag for capillary electrophoresis-mass spektrometry analysis of oligosaccharides and N-linked glycans
Partyka, Jan; Křenková, Jana; Čmelík, Richard; Foret, František
2018 - English
Various basic amino acids and peptides were studied as an alternative labels for capillary electrophoresis-mass spectrometry analysis of oligosaccharides. The hexahistidine-based label with big number of positive charges allowed to use effective and sensitive CE-MS analysis in positive ion mode both neutral and acidic glycans.
Keywords:
CE-MS analysis; oligosaccharides; amino acids; peptides
Fulltext is available at external website.
Hexahistidine-based tag for capillary electrophoresis-mass spektrometry analysis of oligosaccharides and N-linked glycans
Various basic amino acids and peptides were studied as an alternative labels for capillary electrophoresis-mass spectrometry analysis of oligosaccharides. The hexahistidine-based label with big number ...
Epitachophoretic separation and concentration of large volume samples
Voráčová, Ivona; Foret, František; Datinská, Vladimíra; Novotný, Jakub; Gheibi, P.; Berka, J.; Astier, Y.
2018 - English
There has been a growing interest in developing isotachophoretic protocols for concentration and purification of DNA as an alternative to the solid phase extraction protocols of DNA preparation. Also concentration of DNA from large volume samples is required. We have developed a new device suitable for separation, concentration and collection of ions from several milliliter sample volumes into microliter fractions. We have used a discontinuous electrolyte system comprising of the leading and terminating electrolytes utilized for concentration of samples containing DNA fragments. The method is simple, fast with high concentration factor and without extensive surface interactions.
Keywords:
epitachophoretic separation; DNA; large sample volumes
Fulltext is available at external website.
Epitachophoretic separation and concentration of large volume samples
There has been a growing interest in developing isotachophoretic protocols for concentration and purification of DNA as an alternative to the solid phase extraction protocols of DNA preparation. Also ...
CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis
Foret, František; Křenková, Jana; Drobníková, Iveta; Klepárník, Karel; Přikryl, Jan
2018 - English
This year the meeting included full texts of 25 oral and 61 poster presentations. The attached conference proceedings, edited by highly cited scientists, documents the quality of the meeting. Similarly to the previous years the lectures were given by outstanding, highly cited, scientists. The list of speakers as well as more detailed information about the conference can be found on its permanent web at www.ce-ce.org.
Keywords:
bioanalysis; separation; biotechnology
Fulltext is available at external website.
CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis
This year the meeting included full texts of 25 oral and 61 poster presentations. The attached conference proceedings, edited by highly cited scientists, documents the quality of the meeting. ...
Vliv inhalovaných nanočástic oxidů kovů na orgány exponovaných myší
Mikuška, Pavel; Večeřa, Zbyněk; Dočekal, Bohumil; Čapka, Lukáš; Křůmal, Kamil; Coufalík, Pavel; Moravec, Pavel; Buchtová, Marcela; Míšek, Ivan; Vrlíková, Lucie; Smutná, Tereza; Dumková, J.; Hampl, A.; Fictum, P.
2018 - Czech
Dospělé myši byly kontinuálně vystaveny nanočásticím oxidů kovů v různých časových intervalech. Na konci experimentu byly orgány myší (plíce, mozek, ledviny, játra a slezina) analyzovány chemickými, biochemickými, histologickými a elektron mikroskopickými analýzami. Adult mice were continuously exposed to metal oxide nanoparticles for different time intervals. At the end of experiment, mice organs (lung, brain, kidney, liver and spleen) were collected for chemical, biochemical, histological and electron microscopical analyses.
Keywords:
nanoparticles; inhalation; mice
Available in digital repository of the ASCR
Vliv inhalovaných nanočástic oxidů kovů na orgány exponovaných myší
Dospělé myši byly kontinuálně vystaveny nanočásticím oxidů kovů v různých časových intervalech. Na konci experimentu byly orgány myší (plíce, mozek, ledviny, játra a slezina) analyzovány chemickými, ...
Hollow fibre-liquid phase microextraction of basic drugs from dried dried blood spots
Dvořák, Miloš; Miková, B.; Šlampová, Andrea; Kubáň, Pavel
2018 - English
Hollow fibre-liquid phase microextraction (HF-LPME) was applied for rapid and efficient extraction of basic drugs from dried blood spots (DBS). Basic drugs from DBS were first extracted into 10 mM NaOH solution, which acted as the HF-LPME donor solution, and simultaneously the neutralized drugs were transferred across the HF and preconcentrated in 10 mM HCl acceptor solution inside the HF lumen. Mixture (1:1, v:v) of 1-ethyl-2-nitrobenzene and dihexyl ether was used for impregnation of the porous HF membrane. Analytes in the acceptor solution were injected directly from the HF into capillary electrophoresis instrument for further separation, detection and quantification. Repeatability of the hyphenated analytical system ranged from 9.8 to 13.8 % (RSD) and enrichment factors of 52 – 78 were obtained for model basic drugs spiked to DBS extracts at a concentration level of 0.1 mg/L.
Keywords:
hollow fibre-liquid phase microextraction; microextraction of basic drugs; dried blood spots
Fulltext is available at external website.
Hollow fibre-liquid phase microextraction of basic drugs from dried dried blood spots
Hollow fibre-liquid phase microextraction (HF-LPME) was applied for rapid and efficient extraction of basic drugs from dried blood spots (DBS). Basic drugs from DBS were first extracted into 10 mM ...
Surface enhanced raman spectroscopy: Detection platforms for sample analysis with and without electrophoretic separation
Týčová, Anna; Přikryl, Jan; Foret, František; Belder, D.
2018 - English
In this work we present two platforms of relatively simple construction for user-friendly sample detection via surface enhanced Raman spectroscopy (SERS). The first platform is designed for direct sample analysis and deals with hydrophilic spots array within a hydrophobic surface. The second presented platform aims on analysis of samples of higher complexity requiring separation step prior the detection. This is achived via microfluidic electrophoretic device with online SERS detection.
Keywords:
raman spectroscopy; SERS; microfluidic electrophoretic device
Fulltext is available at external website.
Surface enhanced raman spectroscopy: Detection platforms for sample analysis with and without electrophoretic separation
In this work we present two platforms of relatively simple construction for user-friendly sample detection via surface enhanced Raman spectroscopy (SERS). The first platform is designed for direct ...
Speciation analysis of As and Hg in biological material
Petry-Podgórska, Inga; Matoušek, Tomáš; Migašová, M.; Zemanová, Veronika; Pavlík, Milan; Pavlíková, D.; Kratzer, Jan
2018 - English
This work was focused on an analysis of arsenic in selected plants and mercury in hair. A combination of HPLC and ICP-MS was used for analysis of biological tissues extracts. The\nspeciation analysis of low molecular arsenic and mercury species like inorganic iAs3+ and iAs5+, methylarsenic and dimethylarsenic forms was carried out with hydride generation (HG)\ntechnique, arsenic peptides and inorganic mercury (iHg2+) and its methylated form (MHg+) - with reverse phase (RP) chromatography and detected by ICP-MS.
Keywords:
mercury; arsenic; speciation
Fulltext is available at external website.
Speciation analysis of As and Hg in biological material
This work was focused on an analysis of arsenic in selected plants and mercury in hair. A combination of HPLC and ICP-MS was used for analysis of biological tissues extracts. The\nspeciation analysis ...
Synthetic approaches for new labeling tags facilitating glycan analysis
Čmelík, Richard; Křenková, Jana; Foret, František
2018 - English
This study is focused on the synthesis of labels based on the pyrene core bearing several ionizable quaternary amines and the efficient carbonyl-reactive group, such as primary amine or hydrazine.
Keywords:
labeling; organic synthesis; glycan
Fulltext is available at external website.
Synthetic approaches for new labeling tags facilitating glycan analysis
This study is focused on the synthesis of labels based on the pyrene core bearing several ionizable quaternary amines and the efficient carbonyl-reactive group, such as primary amine or hydrazine.
Oxidační potenciál atmosferického aerosolu jako indikátor tvorby ROS
Barboříková, Hana; Mikuška, Pavel; Hegrová, J.; Krajčovič, J.
2018 - Czech
Částice atmosferického aerosolu (PM) ovlivňují naše životní prostředí a především lidské zdraví, jelikož obsahují toxické látky. Účelem této studie je určit celkovou koncentraci a rozpustné frakce vybraných kovů a také příspěvek kovů rozpustných v plicních tekutinách k celkovému oxidačnímu potenciálu PM. Atmospheric particulate matter (PM) affects our environment especially human health because it contains many toxic chemical substances. The purpose of this study is to determine a total content and a soluble fraction of selected metals and also the contribution of metals soluble in pulmonary fluids to a total oxidative potential of PM.
Keywords:
aerosol; oxidative potential; metal
Available in digital repository of the ASCR
Oxidační potenciál atmosferického aerosolu jako indikátor tvorby ROS
Částice atmosferického aerosolu (PM) ovlivňují naše životní prostředí a především lidské zdraví, jelikož obsahují toxické látky. Účelem této studie je určit celkovou koncentraci a rozpustné frakce ...
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