No (307)
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307
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ANTIBACTERIAL EFFECT OF SELECTED NANOPARTICLES AS REVEALED BY DOUBLING TIME OF TREATED ANTHOMONAS CAMESTRIS PV. CAMPESTRIS CULTURES
Pečenka, J.; Svobodová, Kateřina; Eichmeier, A.; Baránek, M.
2016 - English
Besides many possibilities of applications of nanoparticles in the field of medicine, diagnostics, molecular biology, bioorganic chemistry or remediation of environment, there is also a potential of employment of nanoparticles as a tool for elimination and control of bacteria invading plant tissue. In this experiment an antibacterial activity of selected nanoparticles based on silver, gold and bimetallic silver/copper was tested on bacteria Xanthomonas campestris pv. campestris (Xcc) (strain 1279a). The strongest inhibitory effect represented by doubling time of treated cultures was measured in the presence of the smallest silver nanoparticles (9 nm) at the highest concentration (5 ppm).
Keywords:
nanoparticles; Xanthomonas campestris; doubling time
Available at various institutes of the ASCR
ANTIBACTERIAL EFFECT OF SELECTED NANOPARTICLES AS REVEALED BY DOUBLING TIME OF TREATED ANTHOMONAS CAMESTRIS PV. CAMPESTRIS CULTURES
Besides many possibilities of applications of nanoparticles in the field of medicine, diagnostics, molecular biology, bioorganic chemistry or remediation of environment, there is also a potential of ...
UNICELLULAR ALGA CHLORELLA AS A FEED SUPPLEMENT, CURRENT EXPERIENCE AND FUTURE PERSPECTIVES
Kotrbáček, V.; Doucha, Jiří; Kuchařová, V.; Doubek, J.
2016 - English
n experiments with farm animals we studied the effect of Chlorella biomass as a feed supplement. In laying hens we found that diet supplemented with 1 % (i.e. 10 g/kg) of dry disintegrated heterotrophically grown Chlorella biomass significantly increased content of carotenoids in egg yolks of 8 experimental animals already after the first week of supplementation (P<0,01). Compared to 8 control hens these differences remained significantly higher until the end of the eight-week experiment. The average carotenoid concentration was 17,3 mu g per g of yolk in control group and 25,3 mu g per g of yolk in experimental animals (P<0,01). Autotrophically grown Chlorella was supplemented to the 30 gilts one month prior the expected first heat in a dose of 1,5 g per animal per day. These sows entered heat earlier and the number of pregnancy was higher in comparison to the 30 control animals. Other studies observed digestibility of selenium organically bound in Se-enriched Chlorella. The level of selenium in blood, colostrum and also tissues of new born piglets was determined. Group of 8 sows was supplemented with Se-enriched alga during the pregnancy and 3 days after parturition at a dose of 0,3 mg of selenium per 1 kg of feed. The same amount of selenium in the form of sodium selenite was administrated to the control group of 8 individuals. Organic form of selenium significantly increased concentration of Se in blood of sows and colostrum (P<0,05). Piglets born to supplemented sows had also significantly higher level of Se in heart, muscles and liver (P<0.01). The use of fresh Chlorella biomass produced outdoors on a dairy farm was experimentally tested. Location near biogas station enables utilization of flue gas as a source of carbon for algal photosynthesis. The liquid fresh biomass applied to dairy cows during the pregnancy at a dose of 15 g dry mass per cow increased the dry matter content in colostrum from 17,5 g per 100 ml of colostrum in control group to 20,5 g per 100 ml of colostrum in supplemented cows (P<0,05). This pilot experiment demonstrated possibilities of cheaper Chlorella production and its use in ruminant nutrition.
Keywords:
Chlorella; feed supplement; flue gas utilization
Available at various institutes of the ASCR
UNICELLULAR ALGA CHLORELLA AS A FEED SUPPLEMENT, CURRENT EXPERIENCE AND FUTURE PERSPECTIVES
n experiments with farm animals we studied the effect of Chlorella biomass as a feed supplement. In laying hens we found that diet supplemented with 1 % (i.e. 10 g/kg) of dry disintegrated ...
Optimization of pilot production technology of lactamases and development of Pichia pastoris technology
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2015 - English
Development and optimization of the production of lactamases for pharmaceutical industry. Cultivation technology and down-stream processing. Development of the cultivation and DSP technology of the yeast Pichia pastoris in the pilot scale.
Keywords:
Pichia cultivation
Available at various institutes of the ASCR
Optimization of pilot production technology of lactamases and development of Pichia pastoris technology
Development and optimization of the production of lactamases for pharmaceutical industry. Cultivation technology and down-stream processing. Development of the cultivation and DSP technology of the ...
Microbial preparation of intracellular oxidoreductases for food purposes
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2015 - English
Development and optimization of pilot cultivations and DSP of induced intracellular oxidoreductases microbial productions.\n\n
Keywords:
enzyme production
Available at various institutes of the ASCR
Microbial preparation of intracellular oxidoreductases for food purposes
Development and optimization of pilot cultivations and DSP of induced intracellular oxidoreductases microbial productions.\n\n
BIOGENIC AND SYNTHETIC AMORPHOUS SILICA - COMPARE AND INTERACTION WITH BACTERIAL SYSTEMS
Fijalkowski, M.; Adach, K.; Marešová, Helena; Kroisová, D.
2015 - English
The aim of the work is to compare the biogenic silica nanoparticles, which have been isolated from rice husks with synthetically produced silica brand Cab-O-Sil LM-150. The comparison is based on an evaluation of the two systems from the point of view of chemical composition, particle size and structure, ability to form clusters of particles and interactions with selected bacterial systems. Methods used for comparison were: SEM, EDX, TEM, FTIR, ICPOES and bacteriological tests. Husks are standardly contaminated by accompanying ions that are important for plant growth, but undesirable for obtaining a quality product. Rice husk used for the silica isolation were purified by boiling in 10% HCl for 2 hours. Rice husks were then washed with distilled water until pH 7. The dried and purified husks were burned in an oven at 650 degrees C of ramping temperature 10 degrees C/min during 2 hours. The obtained product has a chemical composition analogous to the synthetic product. Biogenic particles reach a size of about 20 nm, about 10 nm synthetic. Both materials have an amorphous structure. Interaction with bacterial systems was performed with Gram-negative bacteria Escherichia coli W3110 strain and Gram-positive bacteria Bacillus cereus. In both cases analogous behaviour was observed depending on the concentration of nanoparticles and reducing the growth rate of cultures of 8% for E. coli and 5% for B. cereus resulted nanoparticles at the concentration of 150 mg/litter of medium.
Keywords:
Biogenic silica; synthetic silica; chemical composition
Available at various institutes of the ASCR
BIOGENIC AND SYNTHETIC AMORPHOUS SILICA - COMPARE AND INTERACTION WITH BACTERIAL SYSTEMS
The aim of the work is to compare the biogenic silica nanoparticles, which have been isolated from rice husks with synthetically produced silica brand Cab-O-Sil LM-150. The comparison is based on an ...
Vývoj technologie pilotní přípravy chitinázy
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2014 - English
Development and optimization of the production of extracellular chitinase by the natural producer and also by recombinant host. Cultivation technology and down-stream processing.
Keywords:
enzyme production; bacterial production; submergeed aerobic cultivation
Available at various institutes of the ASCR
Vývoj technologie pilotní přípravy chitinázy
Development and optimization of the production of extracellular chitinase by the natural producer and also by recombinant host. Cultivation technology and down-stream processing.
Seeking Novel Nitrilase Enzyme (Nitrile Aminohydrolase, EC 3.5.5.1)
Martínková, Ludmila; Kaplan, Ondřej; Veselá, Alicja Barbara
2014 - English
Our research group has created a library of novel nitrilases. In general, the enzymes are available from heterologous producers in amounts ranging from 10 to 20 mg of protein exhbiting 25-50 % purity. The enzymes differ from each other in substrate specificity, temperature optimum, enantioselectivity (arylacetonitrilases) etc. Preferential substrates are phenylacetonitrile, (R,S)-mandelonitrile, benzonitrile or cyanopyridines (depending on the enzyme).
Keywords:
Nitrilase; Expression; Purification
Available at various institutes of the ASCR
Seeking Novel Nitrilase Enzyme (Nitrile Aminohydrolase, EC 3.5.5.1)
Our research group has created a library of novel nitrilases. In general, the enzymes are available from heterologous producers in amounts ranging from 10 to 20 mg of protein exhbiting 25-50 % purity. ...
Gut microbiome and colorectal cancer
Tlaskalová-Hogenová, Helena; Klimešová, Klára; Zákostelská, Zuzana; Kverka, Miloslav; Hornová, Michaela; Vannucci, Luca; Štěpánková, Renata; Hudcovic, Tomáš; Kozáková, Hana; Rossmann, Pavel
2014 - English
The work deals with metagenomic approaches that are currently being used to decipher the genome of the microbiota (microbiome), and, in parallel, functional studies are being performed to analyze the effects of microbiota on the host.
Keywords:
gut; colorectal; cancer
Available at various institutes of the ASCR
Gut microbiome and colorectal cancer
The work deals with metagenomic approaches that are currently being used to decipher the genome of the microbiota (microbiome), and, in parallel, functional studies are being performed to analyze the ...
Vývoj technologií lyopfilizace buněčných lyzátů
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2014 - English
Development and optimization of lyophilization methods of the cell content avoiding loss of functionality.
Keywords:
lyophyllization; cell content; biotechnology
Available at various institutes of the ASCR
Vývoj technologií lyopfilizace buněčných lyzátů
Development and optimization of lyophilization methods of the cell content avoiding loss of functionality.
Microbial and molecular biological analysis of technological solutions from Glanzstoff Bohemia s.r.o
Brennerová, Mária
2014 - English
Main goal of the study was identification of the bacterial contaminations during the production of high-tenacity viscose filaments. The work describes microbial cultivations, isolation of bacteria from technological solutions, and taxonomical identification through sequencing of 16S ribosomal RNA genes and searching for affiliations with bacterial relatives in the NCBI website.
Keywords:
viscose; technological solutions; bacterial isolates; identification
Available at various institutes of the ASCR
Microbial and molecular biological analysis of technological solutions from Glanzstoff Bohemia s.r.o
Main goal of the study was identification of the bacterial contaminations during the production of high-tenacity viscose filaments. The work describes microbial cultivations, isolation of bacteria ...
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