Yes (164)
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164
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Simple flow-focusing microfluidic chip for droplet generation
Křivánková, Jana; Basova, E. Y.; Foret, František
2015 - English
There is an ascending requirement for analysis of chemical content of individual cells involving combination of various bioanalytical and optical sensors and arrangements. At the beginning of the cell analysis there is a need for creation of suitable transport vessels - droplets - that can be easily counted, sorted and serve for manipulation of cells with preserved biocompability as well. Among all of bioanalytical approaches droplet-based microfluidics proved its potential for producing droplets with precisely controlled size, shape as well as for encapsulation of reactants and delivering drugs. This study presents a simple microfluidic chip with flow-focusing geometry for generation of water droplets in fluorocarbon oil.
Keywords:
microfluidic chip; droplet generation; fluorinated oil phase
Fulltext is available at external website.
Simple flow-focusing microfluidic chip for droplet generation
There is an ascending requirement for analysis of chemical content of individual cells involving combination of various bioanalytical and optical sensors and arrangements. At the beginning of the cell ...
Efficient generation of arsanes coupled with HPLC or with cryotrapping for speciation analysis of arsenic
Marschner, Karel; Musil, Stanislav; Rychlovský, P.; Dědina, Jiří
2015 - English
Arsenic speciation analysis by HPLC postcolumn hydride generation or with cryotrapping and detection by atomic fluorescence spectrometry.
Keywords:
arsenic speciation analysis; hydride generation; HPLC
Available in digital repository of the ASCR
Efficient generation of arsanes coupled with HPLC or with cryotrapping for speciation analysis of arsenic
Arsenic speciation analysis by HPLC postcolumn hydride generation or with cryotrapping and detection by atomic fluorescence spectrometry.
Comparison of ionisation properties of aetma-labeled saccharides with common labels
Partyka, Jan; Foret, František
2015 - English
We have tested (2-aminoethyl)trimethylammonium (AETMA) as a label for analysis of oligosaccharides by capillary electrophoresis with electrospray (ESI) mass spectrometry detection and compared its performance to taurine, 2-aminobenzoic acid, 2-aminobenzamide and 8-aminopyrene-1,3,6-trisulfonic acid. The AETMA-labeled saccharides were provided higher ionization signals in positive mode than native sodium or ammonium adducts and equal or higher signals than saccharides labeled by the more commonly used labels tested in this study.
Keywords:
saccharides; AETMA; ESI/MS
Fulltext is available at external website.
Comparison of ionisation properties of aetma-labeled saccharides with common labels
We have tested (2-aminoethyl)trimethylammonium (AETMA) as a label for analysis of oligosaccharides by capillary electrophoresis with electrospray (ESI) mass spectrometry detection and compared its ...
System for fast analysis of explosives in the enviromnemt
Čapka, Lukáš; Večeřa, Zbyněk; Mikuška, Pavel; Šesták, Jozef; Kahle, Vladislav
2015 - English
Detection of explosives is the subject of many investigations by law enforcement agents and forensic scientist. The identification and discrimination of evidence taken from crime scenes is a common practice in forensic investigations. The higher demands on simple and fast determination of hazard materials or strange objects are required in connection with worldwide often repeated terrorist activities.
Keywords:
explosives; hazard materials; energetic materials; portable device for the analysis
Fulltext is available at external website.
System for fast analysis of explosives in the enviromnemt
Detection of explosives is the subject of many investigations by law enforcement agents and forensic scientist. The identification and discrimination of evidence taken from crime scenes is a common ...
Thiol-ene-based monolithic enzymatic microreactor for glycoprotein deglycosylation
Novotný, Jakub; Lafleur, J. P.; Kutter, J. P.; Foret, František
2015 - English
Enzymatic microreactor was fabricated from thiol-ene polymer. Glycoprotein samples were deglycosylated by enzyme PNGase F which was immobilized on the monolith.
Keywords:
thiol-enes; mictoreactor; monolith
Fulltext is available at external website.
Thiol-ene-based monolithic enzymatic microreactor for glycoprotein deglycosylation
Enzymatic microreactor was fabricated from thiol-ene polymer. Glycoprotein samples were deglycosylated by enzyme PNGase F which was immobilized on the monolith.
HILIC separation of AETMA-labeled glycans on a wide bore silica-based monolithic capillary column
Šesták, Jozef; Křenková, Jana; Moravcová, Dana; Planeta, Josef; Kahle, Vladislav
2015 - English
In this contribution, we report the separation of AETMA-labeled glycans on a wide bore (320-μm i. d.) silica-based monolithic capillary column modified by [2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide.
Keywords:
monolithic capillary column
Fulltext is available at external website.
HILIC separation of AETMA-labeled glycans on a wide bore silica-based monolithic capillary column
In this contribution, we report the separation of AETMA-labeled glycans on a wide bore (320-μm i. d.) silica-based monolithic capillary column modified by ...
Synthesis and analysis of quantum dot conjugates intended for fret sensor
Datinská, Vladimíra; Klepárník, Karel; Belšánová, Barbora; Minárik, M.; Foret, František
2015 - English
Our work is focused on synthesis of sensor based on Fӧrster resonance energy transfer (FRET), which is aimed to be used for DNA mutation detection. The sensor is based on the attachment of laboratory synthesized quantum dot (QD) to ssDNA. A complementary chain of a sample is labeled by a fluorescent dye. Thus, only if dsDNA between both complementary chains is formed, the energy from QD (donor) can be transferr ed to the dye acceptor) and FRET is observed.
Keywords:
quantum dots; Fӧrster resonance energy transfer; DNA mutation detection
Fulltext is available at external website.
Synthesis and analysis of quantum dot conjugates intended for fret sensor
Our work is focused on synthesis of sensor based on Fӧrster resonance energy transfer (FRET), which is aimed to be used for DNA mutation detection. The sensor is based on the attachment of ...
Nanostructured gold electrodes for determination of glucose in blood
Farka, Z.; Juřík, T.; Kovář, D.; Podešva, Pavel; Foret, František; Skladal, P.
2015 - English
Fast and sensitive devices for monitoring of glucose are crucial in today’s medicine. Most of the commercial glucometers are currently based on enzymatic catalysis which provides reasonable sensitivity but has a drawback in limited enzyme stability. With the development of novel surfaces, new generation of non-enzymatic sensors characterized by high sensitivity and stability is emerging. In this work, gelatin-templated nanostructured gold electrodes were developed and applied for non-enzymatic glucose determination. The analysis was done by direct electrochemical oxidation during cyclic voltammetry and amperometry with the limit of detection at 1.3 μM. The ability to detect physiological levels glucose in presence of interferents was demonstrated on deproteinized human blood serum.
Keywords:
monitoring of glucose; glucometers; non-enzymatic glucose determination; human blood serum
Fulltext is available at external website.
Nanostructured gold electrodes for determination of glucose in blood
Fast and sensitive devices for monitoring of glucose are crucial in today’s medicine. Most of the commercial glucometers are currently based on enzymatic catalysis which provides reasonable ...
Metal concentrations in urban aerosol in Brno and in exhaust fumes
Coufalík, Pavel; Mikuška, Pavel; Křůmal, Kamil; Vojtíšek, M.; Matoušek, Tomáš; Večeřa, Zbyněk
2015 - English
Total metal concentrations in aerosol samples and exhaust fumes as well as the bioaccessibility of metals in simulated lung fluids were determined. The extraction in vitro seems not to be dependent on the surface tension of extraction solution.
Keywords:
PM1; bioavailability; metals
Fulltext is available at external website.
Metal concentrations in urban aerosol in Brno and in exhaust fumes
Total metal concentrations in aerosol samples and exhaust fumes as well as the bioaccessibility of metals in simulated lung fluids were determined. The extraction in vitro seems not to be dependent ...
CECE 2015. 12th International Interdisciplinary Meeting on Bioanalysis
Foret, František; Křenková, Jana; Drobníková, Iveta; Klepárník, Karel
2015 - English
The issue contains conference program, full texts of the 29 invited lectures with short information about the speakers and their professional carreer, full texts of 49 posters.
Keywords:
bioanalysis; separation; biotechnology
Fulltext is available at external website.
CECE 2015. 12th International Interdisciplinary Meeting on Bioanalysis
The issue contains conference program, full texts of the 29 invited lectures with short information about the speakers and their professional carreer, full texts of 49 posters.
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