Unique exposure system for the whole body inhalation experiments with small animals
Večeřa, Zbyněk; Mikuška, Pavel; Dočekal, Bohumil; Vojta, Šimon; Míšek, Ivan; Moravec, Pavel; Smolík, Jiří
2011 - English
The especially inhalation chamber is constructed for chronic exposure studies of small animals to nanoparticles under strictly controlled conditions in frame of project „Study of transport of inhalated nano-sized particles (Pb, Cd) and their allocation in organs“. The inhalation chamber builds from a glass and stainless steel and makes possible long-term inhalation procedures (up to 60 days, 24 hours/day) with up to four discrete groups of mice population (up to 60 individuals each) or of rats population (up to 20 individuals each) under controlled illumination, temperature, relative humidity, doses (0.5 x 106 - 5 x 106 particles/cm3) and size (10-60 nm) of nano-sized particles which are synthesized via aerosol route in a hot wall tube flow reactor using metal organic precursors.
Keywords:
inhalation chamber; chronic exposure studies of small animals; long-term inhalation procedures
Available at various institutes of the ASCR
Unique exposure system for the whole body inhalation experiments with small animals
The especially inhalation chamber is constructed for chronic exposure studies of small animals to nanoparticles under strictly controlled conditions in frame of project „Study of transport of ...
Unique exposure system for the whole body inhalation experiments with small animals
Večeřa, Zbyněk; Mikuška, Pavel; Moravec, Pavel; Smolík, Jiří
2011 - English
The especially inhalation chamber is constructed for chronic exposure studies of small animals to nanoparticles under strictly controlled conditions in frame of project „Study of transport of inhalated nano-sized particles (Pb, Cd) and their allocation in organs“. The inhalation chamber builds from a glass and stainless steel and makes possible long-term inhalation procedures (up to 60 days, 24 hours/day) with up to four discrete groups of mice population (up to 60 individuals each) or of rats population (up to 20 individuals each) under controlled illumination, temperature, relative humidity, doses (0.5 x 106 - 5 x 106 particles/cm3) and size (10-60 nm) of nano-sized particles which are synthesized via aerosol route in a hot wall tube flow reactor using metal organic precursors.
Keywords:
inhalation chamber; chronic exposure studies of small animals; long-term inhalation procedures
Available at various institutes of the ASCR
Unique exposure system for the whole body inhalation experiments with small animals
The especially inhalation chamber is constructed for chronic exposure studies of small animals to nanoparticles under strictly controlled conditions in frame of project „Study of transport of ...
Seasonal variation in chemical composition of submicron urban aerosol in Brno
Mikuška, Pavel; Křůmal, Kamil; Večeřa, Zbyněk; Vojtěšek, Martin
2011 - English
Submicron aerosol particles in the size fraction PM1 collected in Brno in winter and summer of 2009 were analysed for both selected inorganic (ions, metals) and organic components (PAHs, hopanes and steranes, monosaccharide anhydrides, monocarboxylic acids). Special attention was paid to analysis of organic molecular markers to identify the major emission sources of aerosols.
Keywords:
submicron aerosol; chemical composition; emission sources
Available at various institutes of the ASCR
Seasonal variation in chemical composition of submicron urban aerosol in Brno
Submicron aerosol particles in the size fraction PM1 collected in Brno in winter and summer of 2009 were analysed for both selected inorganic (ions, metals) and organic components (PAHs, hopanes and ...
Immunofluorescent labeling of tissues and free cells via QD-protein conjugates
Lišková, Marcela; Voráčová, Ivona; Hezinová, Věra; Klepárník, Karel; Foret, František
2011 - English
Preparation of quantum dots and using their QD-protein conjugates in immunofluorescent labeling of cells and tissues. For conjugation was used method viaEDC/sulfo-NHS zero-length cross-linkers. We linked QD with annexin V, anti-CD3 and anti-PCNA. We labeled T-lymphocytes, apoptotic lymphocyte, mouse duodenum tissue and tumor tissue.
Keywords:
quantum dots; cell labeling; conjugation
Available at various institutes of the ASCR
Immunofluorescent labeling of tissues and free cells via QD-protein conjugates
Preparation of quantum dots and using their QD-protein conjugates in immunofluorescent labeling of cells and tissues. For conjugation was used method viaEDC/sulfo-NHS zero-length cross-linkers. We ...
Preparation of highly luminescent CdTe quantum dots and its probes
Voráčová, Ivona; Lišková, Marcela; Patrmanová, M.; Klepárník, Karel; Foret, František
2011 - English
Luminescence probes consisting of quantum dots conjugated with macrocyclic ligand complex were characterized by CE-LIF.
Keywords:
quantum dots; capillary electrophoresis; luminescent probes
Available at various institutes of the ASCR
Preparation of highly luminescent CdTe quantum dots and its probes
Luminescence probes consisting of quantum dots conjugated with macrocyclic ligand complex were characterized by CE-LIF.
MSB 2010. Final Program and Book of Abstracts. 25 th International Symposium on Microscale BioSeparations
Foret, František
2010 - English
The issue contains Conference Program, Lecture Abstracts, Poster Abstracts and Author's Index.
Keywords:
bioanalysis; separation; biotechnology
Available at various institutes of the ASCR
MSB 2010. Final Program and Book of Abstracts. 25 th International Symposium on Microscale BioSeparations
The issue contains Conference Program, Lecture Abstracts, Poster Abstracts and Author's Index.
Monosaccharide anhydrides in PM1 and PM2.5 aerosol in urban areas
Křůmal, Kamil; Večeřa, Zbyněk; Mikuška, Pavel
2010 - English
Contribution deals with comparison of concentrations of monosacharide anhydrides (markers for biomass combustion) in Brno and Šlapanice at two seasons (winter and summer) and discussion about their emission sources.
Keywords:
monosaccharide anhydrides; levoglucosan; mannosan; biomass burning
Available at various institutes of the ASCR
Monosaccharide anhydrides in PM1 and PM2.5 aerosol in urban areas
Contribution deals with comparison of concentrations of monosacharide anhydrides (markers for biomass combustion) in Brno and Šlapanice at two seasons (winter and summer) and discussion about their ...
Arsenic speciation analysis by hydride generation – cryotrapping – atomic fluorescence spectrometry with flame-in-gas-shield atomizer
Svoboda, Milan; Musil, Stanislav; Matoušek, Tomáš; Rychlovský, P.; Dědina, Jiří
2010 - English
The main target of this work was further improvement of detection limits in the method of arsenic speciation analysis of tri- and pentavalent human metabolites in complex biological matrices. The method combines selective hydride generation with preconcentration and subsequent separation of substituted arsines in a cryogenic trap.
Keywords:
arsenic speciation; cryogenic trapping; atomic fluorescence spectrometry
Available at various institutes of the ASCR
Arsenic speciation analysis by hydride generation – cryotrapping – atomic fluorescence spectrometry with flame-in-gas-shield atomizer
The main target of this work was further improvement of detection limits in the method of arsenic speciation analysis of tri- and pentavalent human metabolites in complex biological matrices. The ...
Study on oligosaccharide composition of wort and beer samples by liquid chromatography/electrospray ionization tandem mass spectrometry
Čmelík, Richard; Berkecz, R.; Janáky, T.; Bobálová, Janette
2010 - English
LC-MS technique was successfully applied to the description of oligosaccharides mixtures in wort and beer samples. Based on the important differences in MS/MS spectra the structural features of coeluted oligosaccharides were found.
Keywords:
HPLC; mass spectrometry; brewing
Available at various institutes of the ASCR
Study on oligosaccharide composition of wort and beer samples by liquid chromatography/electrospray ionization tandem mass spectrometry
LC-MS technique was successfully applied to the description of oligosaccharides mixtures in wort and beer samples. Based on the important differences in MS/MS spectra the structural features of ...
Arsenic speciation analysis by cryogenic trapping – hydride generation – atomic absorption spectrometry; Investigation of water vapour dryers
Svoboda, Milan; Taurková, Petra; Matoušek, Tomáš; Rychlovský, P.; Dědina, Jiří
2010 - English
The general aim of this work was to contribute to further improvement of the method for complete speciation analysis of trivalent and pentavalent human metabolites of arsenic in complex biological matrices.
Keywords:
arsenic speciation; cryogenic trapping; atomic absorption spectrometry
Available at various institutes of the ASCR
Arsenic speciation analysis by cryogenic trapping – hydride generation – atomic absorption spectrometry; Investigation of water vapour dryers
The general aim of this work was to contribute to further improvement of the method for complete speciation analysis of trivalent and pentavalent human metabolites of arsenic in complex biological ...
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