Vývoj technologií lyopfilizace buněčných lyzátů
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2014 - English
Development and optimization of lyophilization methods of the cell content avoiding loss of functionality.
Keywords:
lyophyllization; cell content; biotechnology
Available at various institutes of the ASCR
Vývoj technologií lyopfilizace buněčných lyzátů
Development and optimization of lyophilization methods of the cell content avoiding loss of functionality.
Microbial and molecular biological analysis of technological solutions from Glanzstoff Bohemia s.r.o
Brennerová, Mária
2014 - English
Main goal of the study was identification of the bacterial contaminations during the production of high-tenacity viscose filaments. The work describes microbial cultivations, isolation of bacteria from technological solutions, and taxonomical identification through sequencing of 16S ribosomal RNA genes and searching for affiliations with bacterial relatives in the NCBI website.
Keywords:
viscose; technological solutions; bacterial isolates; identification
Available at various institutes of the ASCR
Microbial and molecular biological analysis of technological solutions from Glanzstoff Bohemia s.r.o
Main goal of the study was identification of the bacterial contaminations during the production of high-tenacity viscose filaments. The work describes microbial cultivations, isolation of bacteria ...
Vývoj kultivačních technologií výroby intracelulárních alkoholdehydrogenáz
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2014 - English
Development and optimization of pilot cultivations and DSP of intracellular recombinant enzyme productions.
Keywords:
enzyme production; bacterial production; submergeed aerobic cultivation
Available at various institutes of the ASCR
Vývoj kultivačních technologií výroby intracelulárních alkoholdehydrogenáz
Development and optimization of pilot cultivations and DSP of intracellular recombinant enzyme productions.
Host interactions and lactobacilli: from fly model to mouse model/ Role of the intestinal lactobacilli in growth promoting in juvenile development
Kozáková, Hana
2014 - English
We confirmed the hypothesis that the intestinal flora supports juvenile growth in mice, and that the bacterial isolate is capable and mice recapitulate promote growth, similarly as complete microflora.
Keywords:
flies; lactobacili; mice
Available at various institutes of the ASCR
Host interactions and lactobacilli: from fly model to mouse model/ Role of the intestinal lactobacilli in growth promoting in juvenile development
We confirmed the hypothesis that the intestinal flora supports juvenile growth in mice, and that the bacterial isolate is capable and mice recapitulate promote growth, similarly as complete ...
Transfer of technology for production of AEH from Sphingomonas sp
Kyslíková, Eva; Palyzová, Andrea; Marešová, Helena; Štěpánek, Václav; Zahradník, Jiří; Bečka, Stanislav; Dobišová, Marie; Kyslík, Pavel
2014 - English
Research report on construction of a recombinant production microorganism, the way of its cultivation in stirred bioreactor, overproduction and isolation of AEH and stabilization of AEH as cross linked enzyme agregate
Keywords:
Alpha aminoacid ester hydrolase; heterologous expression; stirred bioreactor
Available at various institutes of the ASCR
Transfer of technology for production of AEH from Sphingomonas sp
Research report on construction of a recombinant production microorganism, the way of its cultivation in stirred bioreactor, overproduction and isolation of AEH and stabilization of AEH as cross ...
Vývoj technologií pilotní přípravy laktamáz a dalších enzymů
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2014 - English
Development and optimization of the production of some enzymes for pharmaceutical industry. Cultivation technology and down-stream processing.
Keywords:
enzyme production; bacterial production; submerged aerobic cultivation; biotechnology
Available at various institutes of the ASCR
Vývoj technologií pilotní přípravy laktamáz a dalších enzymů
Development and optimization of the production of some enzymes for pharmaceutical industry. Cultivation technology and down-stream processing.
BATCH BIOREACTORS: WASTE WATER ENDOCRINE DISRUPTOR BIODEGRADATION BY WHITE ROT FUNGI
Křesinová, Zdena; Cajthaml, Tomáš
2013 - English
Endocrine disruptors (EDs) are members of a broad class of environmental organopollutants which are present in the environment. Due to the widespread presence in the surface water and wastewater, and their negative endocrine disrupting activity even at very low concentration, these EDs have received an increased attention in water quality management and health care. EDs encompass a variety of substances, including mammalian hormones (estrogens, androgens) and anthropogenic chemicals
Keywords:
organopollutants
Available at various institutes of the ASCR
BATCH BIOREACTORS: WASTE WATER ENDOCRINE DISRUPTOR BIODEGRADATION BY WHITE ROT FUNGI
Endocrine disruptors (EDs) are members of a broad class of environmental organopollutants which are present in the environment. Due to the widespread presence in the surface water and wastewater, and ...
TEMPERATURE INFLUENCE ON DESORPTION AND DETERMINATION OF BIOACCESSIBLE PAHS
Čvančarová, Monika; Pokorná, Lenka; Velebová, R.; Najmanová, P.; Cajthaml, Tomáš
2013 - English
Hydrophobic organic pollutants tend to adsorb onto soil particles which can cause a significant decrease in their bioaccessible fractions. Definition of the bioaccessibility is currently not unified; however it represents a part of total PAHs amount which is potentially available for biological processes. It is an important parameter which can be used for prediction of microbial degradation and environmental risk evaluation. This paper studies desorption behavior of PAHs at 20 and 60˚C. The desorption of PAHs from long-term contaminated soils was evaluated using resin Amberlite XAD- 2. A two-phase desorption model was used to calculate the desorption kinetic curves and F fractions which represent bioaccessible amount of PAHs. The results showed that the desorption of PAHs was significantly influenced by temperature. The greatest differences in the desorption behavior were observed at 5–6 ring PAHs. We compared the F fractions and the rate constants of individual PAHs which were determined at 20 and 60˚C. The results were also compared with sequential supercritical fluid extraction which has been already used for prediction of microbial degradation.
Keywords:
pahs
Available at various institutes of the ASCR
TEMPERATURE INFLUENCE ON DESORPTION AND DETERMINATION OF BIOACCESSIBLE PAHS
Hydrophobic organic pollutants tend to adsorb onto soil particles which can cause a significant decrease in their bioaccessible fractions. Definition of the bioaccessibility is currently not unified; ...
ENDOCRINE DISUPTIVE CHEMICALS: MECHANISMS OF WHITE ROT FUNGI BIODEGRADATION
Cajthaml, Tomáš; Křesinová, Zdena; Čvančarová, Monika; Maternová, H.; Filipová, A.
2013 - English
The main aim of this study was to elucidate mechanisms involved in EE2 and other EDs compounds degradation by white rot fungi. Results showed simultaneous involvement of ligninolytic enzymes and cytochrome P-450 in biodegradation. The present results indicate the advantage of utilization of WRF cultures for organopollutants degradation.
Keywords:
biodegradation
Available at various institutes of the ASCR
ENDOCRINE DISUPTIVE CHEMICALS: MECHANISMS OF WHITE ROT FUNGI BIODEGRADATION
The main aim of this study was to elucidate mechanisms involved in EE2 and other EDs compounds degradation by white rot fungi. Results showed simultaneous involvement of ligninolytic enzymes and ...
A NOVEL BIOAUGMENTATION APPROACH IN BIODEGRADATION OF ORGANIC POLLUTANTS IN CONTAMINATED SOIL
Lhotský, O.; Filipová, Alena; Innemanová, P.; Velebová, R.; Cajthaml, Tomáš
2013 - English
Polycyclic aromatic hydrocarbons (PAHs) belong among organic compounds that accumulate in the environment mainly resulting from human activities. Because of their potentially negative effects on human health, methods of their removal from the environment are intensively studied. In this work, a biodegradation method of bioaugmentation based on sequential stepwise mixing of inoculated soil with non inoculated soil was used. The subject of this work was an application of a bacterial consortium on a real contaminated (7399 mg.kg-1 of PAHs) soil. 8 bacterial strains isolated from other contaminated sites were inoculated. DNA was isolated from the samples during the inoculation process and the degradation of PAHs was monitored after 4 months.
Keywords:
soil
Available at various institutes of the ASCR
A NOVEL BIOAUGMENTATION APPROACH IN BIODEGRADATION OF ORGANIC POLLUTANTS IN CONTAMINATED SOIL
Polycyclic aromatic hydrocarbons (PAHs) belong among organic compounds that accumulate in the environment mainly resulting from human activities. Because of their potentially negative effects on human ...
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