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A novel nanospray liquid junction interface for versatile CE-MS
Křenková, Jana; Haselberg, R.; Somsen, G. W.; Foret, František
2016 - anglický
In this work we present a new polyimide-based liquid junction nanospray interface, combining the advantages of sheath flow and sheathless designs. The liquid junction interface was prepared as a hybrid microfabricated plastic liquid junction sprayer allowing to perform analysis in standard (30-100 micro m ID) separation capillaries. The interface incorporates a self-aligning port for coupling of the separation capillary as well as ports for automated flushing of both the liquid junction and the separation capillary. The interface permits performing CE both with and without electroosmotic flow. The use of large bore capillaries allows injection and preconcentration of larger sample volumes, favoring detection sensitivity. Different compositions of the spray liquid and BGE can be used when needed.
Klíčová slova:
capillary electrophoresis; mass spectrometry; liquid junction interface
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
A novel nanospray liquid junction interface for versatile CE-MS
In this work we present a new polyimide-based liquid junction nanospray interface, combining the advantages of sheath flow and sheathless designs. The liquid junction interface was prepared as a ...
Screening of acyclovir permeation through skin using alaptide
Bobálová, Janette; Černíková, A.; Bobáľ, P.; Jampílek, J.
2016 - anglický
This investigation deals with the affection of the permeation of acyclovir through fullthickness\npig ear skin using a Franz diffusion cell from the donor vehicle of propylene\nglycol/water (1:1) using synthesised (S)-8-methyl-6,9-diazaspiro[4.5]decane-7,10-\ndione, alaptide as a potential transdermal permeation enhancer.
Klíčová slova:
acyclovir permeation; chemical permeation enhancers; skin permeation experiments
Plné texty jsou dostupné na vyžádání prostřednictvím repozitáře Akademie věd.
Screening of acyclovir permeation through skin using alaptide
This investigation deals with the affection of the permeation of acyclovir through fullthickness\npig ear skin using a Franz diffusion cell from the donor vehicle of propylene\nglycol/water (1:1) ...
Determination of levoglucosan in urban aerosol
Coufalík, Pavel; Mikuška, Pavel; Čmelík, Richard
2016 - anglický
The main aim of this work was a development of the analytical method for levoglucosan\ndetermination including direct collection of aerosol into water phase, preconcentration,\nand determination by LC-MS.
Klíčová slova:
monosaccharide anhydrides; urban aerosol; levoglucosan
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Determination of levoglucosan in urban aerosol
The main aim of this work was a development of the analytical method for levoglucosan\ndetermination including direct collection of aerosol into water phase, preconcentration,\nand determination by ...
Effect of two retinoic acid isomers on the expression of selected nuclear retinoid receptors and protein pattern in human MCF-7 breast cancer cell line
Brtko, J.; Toporová, L.; Macejová, D.; Bialešová, L.; Flodrová, Dana; Bobálová, Janette
2016 - anglický
Effect of two retinoic acid isomers on RARalpha, RARgamma, RXRalpha, RXRbeta expression and protein profile in human MCF-7 breast cancer line were investigated.
Klíčová slova:
retinoic acid; retinoid receptors; protein pattern
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Effect of two retinoic acid isomers on the expression of selected nuclear retinoid receptors and protein pattern in human MCF-7 breast cancer cell line
Effect of two retinoic acid isomers on RARalpha, RARgamma, RXRalpha, RXRbeta expression and protein profile in human MCF-7 breast cancer line were investigated.
Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
Ledvina, Vojtěch; Klepárník, Karel
2016 - anglický
Caspases are proteases that play key role in the process of apoptosis, the programmed\ncell death. Among them, caspase-3 and -7 are main executioner caspases that cleave\nmany vital proteins during apoptosis and after their widespread activation, the process\ncannot be reversed. To analyze caspase-3/7 activation within single cells, a miniaturized\ndevice for parallel analysis of eight samples was developed. The assay is based on the\nmodified luciferin-firefly luciferase bioluminescence (BL) system. Individual\nsuspended cells were collected and transferred into detection microvials using a\nmicromanipulator. The bioluminescence was detected using a photon counting head\nwith cooled photcathode. The LOD suitable for detection of active caspase-3/7 in both\napoptotic and non-apoptotic cells was reached.
Klíčová slova:
single-cell analysis; apoptotic cells; non-apoptotic cells
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
Caspases are proteases that play key role in the process of apoptosis, the programmed\ncell death. Among them, caspase-3 and -7 are main executioner caspases that cleave\nmany vital proteins during ...
Novel, versatile capillary electrophoresis instrument with laser induced fluorescence for analysis of various lipid peroxidation biomarkers
Lačná, J.; Foret, František; Kubáň, Petr
2016 - anglický
In this work we have developed a new sensitive method for quantitation of MDA in\nbiological samples by CE-LIF with LOD about 1.7 nM. First part of the work was\ndevoted to the construction of a new CE-LIF system. Then the derivatization process\nof MDA by TBA was optimized. The ability to use this method for the detection of\nMDA in a biological samples, such as EBC and plasma, was also demonstrated. For the\nfirst time it was possible to detect MDA in EBC samples by CE. Other biomarkers are\nnow being studied.
Klíčová slova:
CE-LIF; malondialdehyde; biomarkers; lipids
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Novel, versatile capillary electrophoresis instrument with laser induced fluorescence for analysis of various lipid peroxidation biomarkers
In this work we have developed a new sensitive method for quantitation of MDA in\nbiological samples by CE-LIF with LOD about 1.7 nM. First part of the work was\ndevoted to the construction of a new ...
Micro-electromembrane extraction across multiple aqueous and organic phases for selective pretreatment of raw biological samples
Kubáň, Pavel; Boček, Petr; Seip, K. F.; Gjelstad, A.; Pedersen-Bjergaard, S.
2016 - anglický
Micro-electromembrane extraction (μ-EME) across multiple aqueous and organic\nsolutions was developed for selective extractions of various analytes from untreated\nbiological samples. The novel approach requires μL volumes of samples and organic\nsolvents, which form consecutive immiscible plugs in a transparent polymeric capillary\nand the extraction process is accelerated by application of d.c. voltage. Number of the\nplugs, their composition, volume and sequence in the extraction capillary can be chosen\nspecifically for each particular application and high variability and selectivity of the\nextraction process can be achieved. In this contribution, we demonstrate suitability of\na five-phase μ-EME system for simultaneous extractions of cations and anions from\nurine and for selective extractions of basic analytes based on their acid-base strength\nfrom plasma.
Klíčová slova:
micro-electromembrane extraction; sample pretreatment techniques; biological samples
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Micro-electromembrane extraction across multiple aqueous and organic phases for selective pretreatment of raw biological samples
Micro-electromembrane extraction (μ-EME) across multiple aqueous and organic\nsolutions was developed for selective extractions of various analytes from untreated\nbiological samples. The novel ...
Characterization of FRET sensor
Datinská, Vladimíra; Klepárník, Karel; Belšánová, B.; Minárik, M.; Foret, František
2016 - anglický
In this study, we present characterization of sensor based on Fӧrster resonance energy\ntransfer (FRET). The sensor is composed of ssDNA chain attached to a laboratory\nsynthesized quantum dot (QD). A complementary chain of a sample is labeled by a\nluminescent dye. When the dsDNA hybrid is formed, the energy from the QD (donor)\nis transferred to the dye (acceptor) and FRET is observed as a decrease of QD\nluminescence emission intensity and an increase of dye luminescence emission\nintensity.
Klíčová slova:
FRET sensor; Fӧrster resonance energy transfer; quantum dot
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Characterization of FRET sensor
In this study, we present characterization of sensor based on Fӧrster resonance energy\ntransfer (FRET). The sensor is composed of ssDNA chain attached to a laboratory\nsynthesized quantum dot (QD). A ...
Microfluidic device for cell counting and characterization
Václavek, Tomáš; Křenková, Jana; Foret, František
2016 - anglický
Integration of cell counting unit is an important step in developing of microfluidic\ndevice for cellular analysis. Here we present a microfluidic device for cell counting\nbased on resistive pulse sensing.
Klíčová slova:
microfluidic devices; microfluidic chip; cellular analysis
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Microfluidic device for cell counting and characterization
Integration of cell counting unit is an important step in developing of microfluidic\ndevice for cellular analysis. Here we present a microfluidic device for cell counting\nbased on resistive pulse ...
Organic markers and compounds in PM1 aerosol in small town near Prague (Czech Republic) in winter 2016
Křůmal, Kamil; Mikuška, Pavel; Coufalík, Pavel; Večeřa, Zbyněk
2016 - anglický
Contribution summarizes the concentrations of monosaccharide anhydrides, resin\nacids, methoxyphenols, monosaccharides, disaccharides, sugar alcohols, alkanes,\nhopanes, steranes and polycyclic aromatic hydrocarbons in PM1 aerosols in small town\nKladno-Švermov in winter. According to concentrations of organic markers, the\nidentification of emission sources in Kladno-Švermov is discussed.
Klíčová slova:
organic markers; PM1 aerosol; combustion of biomass; aerosols collected
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Organic markers and compounds in PM1 aerosol in small town near Prague (Czech Republic) in winter 2016
Contribution summarizes the concentrations of monosaccharide anhydrides, resin\nacids, methoxyphenols, monosaccharides, disaccharides, sugar alcohols, alkanes,\nhopanes, steranes and polycyclic ...
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