24-Epibrassinolide at Subnanomolar Concentrations Modulates Growth of Mouse Hybridoma Cells
Franěk, František; Kohout, Ladislav; Eckschlager, T.
2003 - English
Brassinolides are known to stimulate plant growth and to posses antistresiTactivities in plants. This work was aimed at exploring possible beneficial effects of 24-epibrassinolide on cultured mammalian cells. A mouse hybridoma, i.e. a hybrid ymphocyte cell line, was cultured either in standard serum-free medium, or in diluted medium in which the cell grew inder nutritional stress. Steady-state parameters of semicon-finuous cultures conducted at 24-epibrassinolide concentra-ions from 10~16 M to 1CT9 M were evaluated. Typical effects of he agent that were observed both in standard and in diluted i [media were suppression of intracellular antibody level, increa- / ,ed value of mitochondrial membrane potential, higher frac- / ion of cells in the G(/Gl phase and lower fraction of cells in / he S phase. Alleviation of nutritional stress was observed in j (cultures conducted in diluted media. Viable cell density was j ignificantly higher at 24-epibrassinolide concentrations 10~13[ knd 10~12 M. Results of this exploratory study show that thej plant hormone 24-epibrassinolide may induce alterations of he cell division mechanism of the energetic metabolism, and of secreted protein synthesis in a mammalian cell line.
Keywords:
Brassinolides; cell division
Available at various institutes of the ASCR
24-Epibrassinolide at Subnanomolar Concentrations Modulates Growth of Mouse Hybridoma Cells
Brassinolides are known to stimulate plant growth and to posses antistresiTactivities in plants. This work was aimed at exploring possible beneficial effects of 24-epibrassinolide on cultured ...
Genetic Variability Between Different Isolates of Potato Mop-top Virus (PMTV)
Rosecká, Pavla; Moravec, Tomáš; Filigarová, Marie; Dědič, P.; Čeřovská, Noemi
2002 - English
We determined partial nucleotide sequences of several different field isolates of PMTV with well-characterized biological properties from the Czech Republic and Denmark. Subsequently we were trying to elucidate the correlation between nucleotide sequences of various isolates, their biological properties and geographical origin. From the comparison with other known sequences we can conclude, that PMTV genome is relatively conserved.
Keywords:
Potato mop-top virus; recombinant coat protein; Escherichia coli
Available at various institutes of the ASCR
Genetic Variability Between Different Isolates of Potato Mop-top Virus (PMTV)
We determined partial nucleotide sequences of several different field isolates of PMTV with well-characterized biological properties from the Czech Republic and Denmark. Subsequently we were trying to ...
Cytological aspects of Lactuca SPP.-Bremia Lactucae interaction
Sedlářová, Michaela; Lebeda, A.; Binarová, Pavla
1999 - English
Available at various institutes of the ASCR
Cytological aspects of Lactuca SPP.-Bremia Lactucae interaction
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