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Synthesis and biological activity of thiophene fused 7-deazapurine ribonucleosides
Tichý, Michal; Hocek, Michal
2014 - English
A series of thieno[2’,3’:4,5]pyrrolo[2,3-d]pyrimidine ribonucleosides bearing various alkyl, amino or hetaryl groups in position 4 has been prepared and all compounds have been tested for cytostatic and antiviral activities (HCV and Dengue viruses).
Keywords:
nucleosides; cytostatics; 7-deazapurine ribonucleosides
Available at various institutes of the ASCR
Synthesis and biological activity of thiophene fused 7-deazapurine ribonucleosides
A series of thieno[2’,3’:4,5]pyrrolo[2,3-d]pyrimidine ribonucleosides bearing various alkyl, amino or hetaryl groups in position 4 has been prepared and all compounds have been tested for cytostatic ...
Ambientní ionizační techniky
Vrkoslav, Vladimír
2014 - Czech
Byly popsány principy nejdůležitějších ambientních ionizačních technik, jejich rozdělení a příklady využití. Classification, principles and application examples of the most important ambient ionization techniques were described.
Keywords:
mass spectrometry; analysis; desorption
Available at various institutes of the ASCR
Ambientní ionizační techniky
Byly popsány principy nejdůležitějších ambientních ionizačních technik, jejich rozdělení a příklady využití....
(MA)LDI MS a MSI malých molekul
Vrkoslav, Vladimír
2014 - Czech
Byla vysvětlena specifika použití MALDI/MS a LDI/MS pro analýzu malých molekul. Using of MALDI/MS and LDI/MS for analysis of small molecular was explained.
Keywords:
mass spectrometry; mass spectrometry imaging; matrix assisted laser desorption/ ionization
Available at various institutes of the ASCR
(MA)LDI MS a MSI malých molekul
Byla vysvětlena specifika použití MALDI/MS a LDI/MS pro analýzu malých molekul.
Using of MALDI/MS and LDI/MS for analysis of small molecular was explained.
Analysis and characterization of antimicrobial peptides by capillary electrophoresis
Tůmová, Tereza; Monincová, Lenka; Čeřovský, Václav; Kašička, Václav
2014 - English
Capillary electrophoresis has been employed for quantitative and qualitative analysis, separation and characterization of antimicrobial peptides – halictines. In addition to purity degree of synthetic peptides, also their effective and ionic mobilities and charges within a wide pH range were determined.
Keywords:
capillary electrophoresis; halictines; physico-chemical parameters
Available at various institutes of the ASCR
Analysis and characterization of antimicrobial peptides by capillary electrophoresis
Capillary electrophoresis has been employed for quantitative and qualitative analysis, separation and characterization of antimicrobial peptides – halictines. In addition to purity degree of synthetic ...
Development and applications of ionization techniques in ambient mass spectrometry
Rejšek, Jan; Vrkoslav, Vladimír; Cvačka, Josef
2014 - English
Ambient mass spectrometry is a sharply developing subject area. Desorption electrospray ionization (DESI) and desorption atmospheric pressure photoionization (DAPPI) are two types of ionization techniques in the ambient mass spectometry. Herein, they were investigated in two research areas: (1) TLC analysis. (2) Localization of insect defense glands.
Keywords:
ambient mass spectrometry; desorption electrospray ionization; desorption atmospheric pressure photoionization; thin-layer chromatography; insect defense compounds; mass spectrometry imaging
Available at various institutes of the ASCR
Development and applications of ionization techniques in ambient mass spectrometry
Ambient mass spectrometry is a sharply developing subject area. Desorption electrospray ionization (DESI) and desorption atmospheric pressure photoionization (DAPPI) are two types of ionization ...
Voltammetric Behavior of 4-Aminophthalimide Label using Hanging Mercury Drop Electrode
Ferenčíková, Z.; Daňhel, Aleš; Riedl, Jan; Hocek, Michal; Fojta, Miroslav
2014 - English
4-aminophthalimide (API), covalently attached to nucleotide (dCTP or dATP) has recently beendesigned as a fluorescent label for the detection of DNA-protein interaction. However API is electrochemically reducible and therefore it is possible to use alternatively the API-modified nuclotides as DNA redox labels. The electrochemical behavior of labeled nucleotides and labeled DNA was studied using cyclic voltammetry and transfer stripping cyclic voltammetry at hanging mercury drop electrode. Observed CVs showed irreversible reduction signals of 4-aminophthalimide label without production of consequently oxidizable/reducible species.
Keywords:
labeled nucleotide; 4-aminophthalimide; primer extension; DNA; hanging mercury drop electrode; voltammetry
Available at various institutes of the ASCR
Voltammetric Behavior of 4-Aminophthalimide Label using Hanging Mercury Drop Electrode
4-aminophthalimide (API), covalently attached to nucleotide (dCTP or dATP) has recently beendesigned as a fluorescent label for the detection of DNA-protein interaction. However API is ...
Voltametrická analýza nukleosidtrifosfátů a oligonukleotidů značených antrochinonem na zlatých elektrodách
Vidláková, Pavlína; Balintová, Jana; Havran, Luděk; Hocek, Michal; Fojta, Miroslav
2014 - Czech
Elektrochemická aktivita nukleových kyselin byla objevena v 50. letech 20. století a od té doby je používána ke studiu struktury a interakcí přirozených i modifikovaných molekul nukleových kyselin i syntetických oligonukleotidů. Přestože je přirozená DNA sama o sobě elektrochemicky aktivní a je možné ji studovat na různých typech elektrod, je pro řadu analytických aplikací praktické použít DNA značenou elektroaktivními molekulami (například komplexy přechodných kovů, amino- nebo nitroskupinami, antrachinonem apod.). Tyto látky podléhají redoxním reakcím a dávají takto modifikované DNA nové elektrochemické vlastnosti, které je možné využít pro studium struktury a interakcí nukleových kyselin a v oblasti DNA diagnostiky. DNA labelling is used for increase of sensitivity of electrochemical detection. When the DNA or oligonucleotides (ONs) are chemically modified, their electrochemical responses can be changed depending on electrochemical activity of the introduced moiety. One option of DNA labeling is incorporation of chemically modified nucleotides using corresponding deoxynucleotide triphosphates (dNTPs) into DNA molecules with using primer extension (PEX). In our study we used anthraquinone labeled dNTPs and ONs with enzymatically incorporated anthraquinone labeled nucleotides. Thus synthesized ON stretch bearing the anthraquinone tags was electrochemically analyzed using voltammetry at gold electrodes.
Keywords:
DNA; anthraquinone; electrochemical analysis; DNA modification; primer extension
Available at various institutes of the ASCR
Voltametrická analýza nukleosidtrifosfátů a oligonukleotidů značených antrochinonem na zlatých elektrodách
Elektrochemická aktivita nukleových kyselin byla objevena v 50. letech 20. století a od té doby je používána ke studiu struktury a interakcí přirozených i modifikovaných molekul nukleových kyselin i ...
Separation of nonpolar lipids from vernix caseosa
Háková, Eva; Míková, Radka; Vrkoslav, Vladimír; Doležal, A.; Plavka, R.; Cvačka, Josef
2014 - English
The aim of this work is to develop a separation method for characterization of unknown nonpolar lipids in vernix caseosa. Here we optimized adsorption chromatography coupled to mass spectrometry detection with exact mass measurement. A long silica gel column (250 + 250 x 4.6 mm, particle size: 5 mu m) provided the best separation of nonpolar lipids present in vernix caseosa.
Keywords:
vernix caseosa; lipids; normal-phase chromatography; mass spectrometry
Available at various institutes of the ASCR
Separation of nonpolar lipids from vernix caseosa
The aim of this work is to develop a separation method for characterization of unknown nonpolar lipids in vernix caseosa. Here we optimized adsorption chromatography coupled to mass spectrometry ...
Capillary electrophoresis applied to chiral analysis of helquats and characterization of their intermediates and interconversion barriers using randomly and single isomer sulfated cyclodextrins as stereoselectors
Kašička, Václav; Koval, Dušan; Sázelová, Petra; Severa, Lukáš; Vávra, Jan; Adriaenssens, Louis; Sonawane, Manoj R.; Teplý, Filip
2014 - English
Capillary electrophoresis in acidic sodium phosphate background electrolyte, pH 2.4, with randomly sulfated alfa-, beta- and gamma-cyclodextrins as chiral selectors was applied to fast, high-resolution separation of enantiomers of a series of 35 helquats, a new class of N-heteroaromatic dicationic helicene-like species. The developed methodology was employed for evaluation of preparative enantioresolution procedures of helquats, for monitoring their racemization and for determination of interconversion barrier of helquat enantiomers.
Keywords:
helquats; chiral analysis; capillary electrophoresis
Available at various institutes of the ASCR
Capillary electrophoresis applied to chiral analysis of helquats and characterization of their intermediates and interconversion barriers using randomly and single isomer sulfated cyclodextrins as stereoselectors
Capillary electrophoresis in acidic sodium phosphate background electrolyte, pH 2.4, with randomly sulfated alfa-, beta- and gamma-cyclodextrins as chiral selectors was applied to fast, ...
Affinity capillary electrophoresis applied for study of solvent effect on stability constant of dibenzo-18-crown-6 complex with potassium ion
Konášová, Renáta; Jaklová Dytrtová, Jana; Kašička, Václav
2014 - English
Affinity capillary electrophoresis was used for investigation of solvent effect on stability constant of dibenzo-18-crown-6 complex with potassium ion in mixed hydro-organic solvents at different water/methanol and water/ethanol ratios. The stability constant of the complex was decreasing with increasing molar fraction of water in mixed solvents and almost independent of the organic solvent used.
Keywords:
affinity capillary electrophoresis; solvent effect; binding constant
Available at various institutes of the ASCR
Affinity capillary electrophoresis applied for study of solvent effect on stability constant of dibenzo-18-crown-6 complex with potassium ion
Affinity capillary electrophoresis was used for investigation of solvent effect on stability constant of dibenzo-18-crown-6 complex with potassium ion in mixed hydro-organic solvents at different ...
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