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Evaluation of the 33 synthesized nitrophenol-based markers of isoelectic point
Duša, Filip; Moravcová, Dana; Šlais, Karel
2018 - English
33 nitrophenol-based compounds, which absorb at ultraviolet as well as visible wavelengths, were selected as candidates for marking isoelectric points (pI) over the pH range from 3 to 10. The evaluation and selection of the candidate structures included structure-based calculations of acid-base properties. The selected compounds were further tested experimentally using capillary isoelectric focusing. The experimental analysis excluded two compounds due to producing double peaks during the analysis. Overall, 31 novel compounds were identified as suitable pI markers.
Keywords:
nitrophenol-based markers; isoelectic point; capillary isoelectric focusing
Fulltext is available at external website.
Evaluation of the 33 synthesized nitrophenol-based markers of isoelectic point
33 nitrophenol-based compounds, which absorb at ultraviolet as well as visible wavelengths, were selected as candidates for marking isoelectric points (pI) over the pH range from 3 to 10. The ...
Chromatographic separation of aflatoxin analogues
Moravcová, Dana; Planeta, Josef; Lunerová, K.; Duša, Filip; Šesták, Jozef; Kubíček, J.; Horká, Marie
2018 - English
Three short particle-packed columns (50 x 2.1 mm) were compared for the rapid and efficient chromatographic separation of aflatoxin G1, G2, B1 and B2 analogues.
Keywords:
aflatoxin; HPLC; C18 stationary phase
Fulltext is available at external website.
Chromatographic separation of aflatoxin analogues
Three short particle-packed columns (50 x 2.1 mm) were compared for the rapid and efficient chromatographic separation of aflatoxin G1, G2, B1 and B2 analogues.
Arduino-based pressure-driven flow controller for droplet microfluidics
Přikryl, Jan; Křivánková, Jana; Hlaváček, Antonín
2018 - English
Droplet-based microfluidic systems bear significant value in an extremely wide range of applications due to its remarkable advantages, nevertheless, their technical demands on precise flow control are high. Here, we present lab-built Arduino-based flow controller fulfilling needs of droplet microfluidics to easily and effectively automate the system. As an additional required accessory, CNC-milled component for vials pressurizing was presented.
Keywords:
droplet microfluidics; microfluidic systems; pressure regulator
Fulltext is available at external website.
Arduino-based pressure-driven flow controller for droplet microfluidics
Droplet-based microfluidic systems bear significant value in an extremely wide range of applications due to its remarkable advantages, nevertheless, their technical demands on precise flow control are ...
Rapid fabrication of microextraction devices for direct determination of basic drugs in dried blood spots
Ryšavá, Lenka; Přikryl, Jan; Dvořák, Miloš; Malá, Zdeňka; Kubáň, Pavel
2018 - English
A 3D-printed cutter was designed for rapid fabrication of supported liquid membrane (SLM) extraction devices compatible with injection system of commercial capillary electrophoresis (CE) instrument. Low cost and single use microextraction devices were fabricated from polypropylene micropipette tips with high precision (dimension variances ≤ 3% RSD). Practical applicability of the microextraction devices was examined by in-line coupling of SLM extractions to CE for direct analysis of four model basic drugs in dried blood spot extracts. Excellent repeatability of the hyphenated technique was achieved (RSD ≤ 7.6%), transfer of analytes across the SLM ranged from 23 to 73% and limits of detection were as low as 0.07 μg/mL.
Keywords:
supported liquid membrane (SLM); capillary electrophoresis; microextraction devices
Fulltext is available at external website.
Rapid fabrication of microextraction devices for direct determination of basic drugs in dried blood spots
A 3D-printed cutter was designed for rapid fabrication of supported liquid membrane (SLM) extraction devices compatible with injection system of commercial capillary electrophoresis (CE) instrument. ...
Maximizing efficiency of field-amplified electrokinetic injection of weak bases for enantioselective capillary electrophoresis with sulfated cyclodextins
Šesták, Jozef; Thormann, W.
2018 - English
The optimal conditions for field-amplified electrokinetic injection of cations followed by their separation based on the stereoselective interaction with sulfated cyclodextrins are discussed. Results show that addition of a short water plug together with a short plug free of cyclodextrins and with a buffer concentration similar to the BGE greatly increase the injection efficiency.
Keywords:
capillary electrophoresis; cyclodextrins
Fulltext is available at external website.
Maximizing efficiency of field-amplified electrokinetic injection of weak bases for enantioselective capillary electrophoresis with sulfated cyclodextins
The optimal conditions for field-amplified electrokinetic injection of cations followed by their separation based on the stereoselective interaction with sulfated cyclodextrins are discussed. Results ...
A New evaluation of combined antitumor effects of natural and synthetic nuclear retinoid receptor ligands in human breast carcinoma cells
Strouhalová, Dana; Laštovičková, Markéta; Bobál, P.; Macejová, D.; Mosná, B.; Brtko, J.; Bobálová, Janette
2018 - English
This work represents the study of the effects of biologically active ligands of nuclear retinoid X receptors, triorganotin compounds, on proteomic profile of MDA-MB-231 cells. We focused primarily on tributyl/phenyltin derivatives that suggest the combined antitumor effect when they are used with natural nuclear retinoid receptor ligand all-trans retinoic acid (ATRA). Proteomic strategies connected to PDQuest evaluation were applied in this study. Some specific proteins with tumor process association were chosen for more detail study. Our findings indicate that selected triorganotins derivatives resulted to significantly reduced level of studied proteins belonging to metabolic pathway or to other cellular processes as apoptosis or epithelial–mesenchymal transition. In the case of vimentin, the key protein of EMT process, the silencing was set up by triorganotin compounds and significantly enhanced by combination with ATRA. This additive effect on downregulation can result from the potential engagement of RXR/RAR heterodimer, considering co-addition of both partner ligands of mentioned heterodimer to the treatment.
Keywords:
nuclear retinoid X receptors; triorganotin compounds; breast carcinoma cells
Fulltext is available at external website.
A New evaluation of combined antitumor effects of natural and synthetic nuclear retinoid receptor ligands in human breast carcinoma cells
This work represents the study of the effects of biologically active ligands of nuclear retinoid X receptors, triorganotin compounds, on proteomic profile of MDA-MB-231 cells. We focused primarily on ...
Fate of smelter dusts in temperate and semi-arid soils
Ettler, V.; Jarošíková, A.; Mihaljevič, M.; Kříbek, B.; Veselovský, F.; Penížek, V.; Vaněk, A.; Šráček, O.; Matoušek, Tomáš; Mapani, B.; Kamona, F.; Nyambe, I.
2017 - English
Our results indicate that, compared to semi-arid areas (where inorganic contaminants are rather immobile in soils despite their high concentrations), a higher potential risk exists in smelter-affected humid areas, where metal(loid) contaminants can be easily released by extensive weathering of particulates in the soil systems.
Keywords:
soils; smelter dusts
Available in digital repository of the ASCR
Fate of smelter dusts in temperate and semi-arid soils
Our results indicate that, compared to semi-arid areas (where inorganic contaminants are rather immobile in soils despite their high concentrations), a higher potential risk exists in smelter-affected ...
Characterization of upconversion nanoparticles by fluorescence spectrometry and capillary electrophoresis
Vaněčková, T.; Zítka, J.; Hlaváček, Antonín; Adam, V.; Vaculovičová, M.
2017 - English
Upconversion nanoparticles (UCNPs) are a novel class of luminescent tags for applications in life and material sciences. Unlike traditional fluorophores, UCNPs exhibit emission of shorter wavelength under near-infrared excitation (typically 980 nm). In this work, we have examined these unique photophysical properties by fluorescence spectrometry and capillary electrophoresis. UCNPs co-doped with Yb(III) and Er(III) were characterized using laboratory-made fluorescence spectrometer. We have exploited and evaluated two excitation sources and the dependence of the fluorescence of UCNPs on the relative excitation power. Moreover, capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection was for the first time used for characterization of the nanoparticles. It was proved that CE-LIF is a valuable method to be used for investigation of upconversion luminiscence and monitoring of the interactions of UCNPs with other molecules of interest.
Keywords:
capillary electrophoresis; fluorescence spectrometry; upconversion nanoparticles
Fulltext is available at external website.
Characterization of upconversion nanoparticles by fluorescence spectrometry and capillary electrophoresis
Upconversion nanoparticles (UCNPs) are a novel class of luminescent tags for applications in life and material sciences. Unlike traditional fluorophores, UCNPs exhibit emission of shorter wavelength ...
CECE 2016. 13th International Interdisciplinary Meeting on Bioanalysis
Foret, František; Křenková, Jana; Drobníková, Iveta; Klepárník, Karel
2016 - English
This year the meeting included full texts of 32 oral and 78 poster presentations delivered by participants from 17 countries. The attached conference proceedings, edited by highly cited scientists, documents the quality of the meeting. Similarly to the previous years the lectures were given by outstanding, highly cited, scientists. The list of speakers as well as more detailed information about the conference can be found on its permanent web at www.ce-ce.org.
Keywords:
bioanalysis; separation; biotechnology
Fulltext is available at external website.
CECE 2016. 13th International Interdisciplinary Meeting on Bioanalysis
This year the meeting included full texts of 32 oral and 78 poster presentations delivered by participants from 17 countries. The attached conference proceedings, edited by highly cited scientists, ...
Development of microfluidic device for droplet generation and microparticle encapsulation
Křivánková, Jana; Foret, František
2016 - English
Microfluidic devices combined with various bioanalytical and optical sensors proved\ntheir potential in chemical, biological, and biomedicine applications. At a droplet\nmicrofluidic device a fluid is divided into numerous droplets surrounded by a\ncontinuous immiscible fluid phase. Generated droplets serve then as\nvessels/microcapsules for delivering drugs, nutrients as well as for encapsulating of\nbiologically active particles and cells.\nDescribed experimental study deals with the development of a practical dropletbased\nmicrofluidic chip useful for production of monodisperse water/oil emulsion and\nfor encapsulation of thin-metal magnetic microsheets.
Keywords:
microfluidic device; microparticle encapsulation
Available in digital repository of the ASCR
Development of microfluidic device for droplet generation and microparticle encapsulation
Microfluidic devices combined with various bioanalytical and optical sensors proved\ntheir potential in chemical, biological, and biomedicine applications. At a droplet\nmicrofluidic device a fluid is ...
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