4th CCP Phenogenomics Conference abstract book
Sedláček, Radislav
2022 - English
The fourth CCP Phenogenomics Conference was held as a hybrid meeting. The scientific committee selected the topic of rare diseases: experimental models & delivery of therapies as the main thematic focus of the 2022 Conference. The Conference provided again an excellent opportunity to support networking and interactions among the researchers, CCP staff, users and experts from the commercial sector.
Keywords:
phenogenomics; rare diseases; gene therapy; experimental models; delivery of therapies
Available at various institutes of the ASCR
4th CCP Phenogenomics Conference abstract book
The fourth CCP Phenogenomics Conference was held as a hybrid meeting. The scientific committee selected the topic of rare diseases: experimental models & delivery of therapies as the main thematic ...
4th CCP Phenogenomics Conference abstract book
Sedláček, Radislav
2022 - English
The fourth CCP Phenogenomics Conference was held as a hybrid meeting. The scientific committee selected the topic of rare diseases: experimental models & delivery of therapies as the main thematic focus of the 2022 Conference. The Conference provided again an excellent opportunity to support networking and interactions among the researchers, CCP staff, users and experts from the commercial sector.
Keywords:
phenogenomics; rare diseases; gene therapy; experimental models; delivery of therapies
Available at various institutes of the ASCR
4th CCP Phenogenomics Conference abstract book
The fourth CCP Phenogenomics Conference was held as a hybrid meeting. The scientific committee selected the topic of rare diseases: experimental models & delivery of therapies as the main thematic ...
3rd CCP Phenogenomics Conference 2021: Abstract Book
Sedláček, Radislav
2021 - English
The Conference was divided into two blocks. The first day was devoted to the theme “Human diseases and models“. The second day was specifically dedicated to preclinical development, including covid-19, which focused on translation of the basic research into the application. The Conference provided an excellent opportunity to support networking and interactions among the CCP users and experts.
Keywords:
phenogenomics; human diseases; mouse models; preclinical development; covid-19
Available at various institutes of the ASCR
3rd CCP Phenogenomics Conference 2021: Abstract Book
The Conference was divided into two blocks. The first day was devoted to the theme “Human diseases and models“. The second day was specifically dedicated to preclinical development, including ...
Binding properties of boar seminal plasma proteins in the reproduction process
Jonáková, Věra; Maňásková, Pavla; Liberda, J.; Tichá, M.
2005 - English
Boar seminal plasma proteins (spermadhesins and DQH protein)bind the sperm surface at ejaculation. binding of sperm to oviductal epithelial cells and to the glycoproteins of zona pellucida are mediated by the protein-saccharide (lectin-like)interactions.These intractions play role in the formation of sperm oviductal reservoir, in the release of capacitated sperm from the surface of oviduct and in sperm-zona pellucida interaction. Proteiny kančí semenné plasmy (spermadhesiny a DQH protein) se váží na povrch spermie při ejakulaci. Vazba spermie k epiteliálním buňkám oviduktu a ke glykoproteinům zony pellucidy je zprostředkována protein-sacharidovými interakcemi (lektin-like).Tyto interakce hrají roli v tvorbě oviduktálního rezervoáru spermií, v uvolnění kapacitovaných spermií z povrchu oviduktu a ve vazbě spermie na zonu pellucidu vajíčka.
Keywords:
seminal plasma proteins; reproduction; binding properties
Available at various institutes of the ASCR
Binding properties of boar seminal plasma proteins in the reproduction process
Boar seminal plasma proteins (spermadhesins and DQH protein)bind the sperm surface at ejaculation. binding of sperm to oviductal epithelial cells and to the glycoproteins of zona pellucida are ...
The origin of boar seminal plasma proteins
Maňásková, Pavla; Kyselová, Vendula; Tichá, M.; Jonáková, Věra
2005 - English
Spermadhesins were detected with their antibodies in boar epididymal fluid, but DQH and beta-microseminoprotein were not. Indirect immunofluorescence technique was employed to determine the distribution of seminal plasma proteins on tissue sections of the reproductive organs. The mRNA of the DQH was found only in seminal vesicles, not in the epididymis, and the mRNA of beta-MSP in testis, seminal vesiclas and prostate. Spermadhesiny byly nalezeny pomocí svých protilátek v kančí epididymální tekutině, ale DQH a beta microseminoprotein nikoli. Nepřímá imunofluorescenční technika byla použita pro odhalení distribuce proteinů semenné plasmy na řezech tkání kančích reprodukčních orgánů. mRNA beta-MSP v testes, semenných vacích a prostatě.
Keywords:
seminal plasma proteins; boar reproductive organs
Available at various institutes of the ASCR
The origin of boar seminal plasma proteins
Spermadhesins were detected with their antibodies in boar epididymal fluid, but DQH and beta-microseminoprotein were not. Indirect immunofluorescence technique was employed to determine the ...
Regulation of ribosome synthesis in bacteria
Krásný, Libor
2005 - English
Rapidly growing bacteria need a high number of ribosomes to satisly the cells need for increased translation. Starving bacteria need a low number of ribosomes to limit energy expenditure.The number of ribosomes is regulated at the level of ribosomal RNA (rRNA) transcription. Rychle rostoucí bakterie potřebují velké množství ribozomů pro pokrytí potřeby zvýšené buněčné translace. Bakterie v podmínkách omezené výživy mají potřebu ribozomů nízkou kvůli šetření energií. Počet ribozomů je regulován na úrovni transkripce ribozomální RNA (rRNA).
Keywords:
ribosome synthesis; bacteria
Available at various institutes of the ASCR
Regulation of ribosome synthesis in bacteria
Rapidly growing bacteria need a high number of ribosomes to satisly the cells need for increased translation. Starving bacteria need a low number of ribosomes to limit energy expenditure.The number of ...
Carbonic anhydrase IX (CA IX) mediates tumor cell interactions with microenvironment
Závada, Jan; Závadová, Zuzana
2004 - English
CA IX zprostředkuje vzájemné reakce nádorových buněk s mikroprostředím několika způsoby: je enzymaticky aktivní, reguluje pH a řídí přenos iontů z/do buňky; CA IX je také adhezivní molekulou zprostředkující mezibuněčný kontakt; slouží jako senzor pH a účastní se invaze a metastáze nádorů CA IX mediates interaction between tumor cells and microenvironment in several ways: it is enzymatically active, it regulates pH and drives ion transport from/to the cell; CA IX is also an adhesion molecule, mediating cell-cell contact; it serves as a pH sensor and it participates in tumor invasion and metastasis
Keywords:
cancer biology; microenvironmnet; carbonic anhydrase IX; nádorové buňky; buňky
Available at various institutes of the ASCR
Carbonic anhydrase IX (CA IX) mediates tumor cell interactions with microenvironment
CA IX zprostředkuje vzájemné reakce nádorových buněk s mikroprostředím několika způsoby: je enzymaticky aktivní, reguluje pH a řídí přenos iontů z/do buňky; CA IX je také adhezivní molekulou ...
Structural Studies of anti-CA IX Monoclonal Antibody M75 Fab Fragment in Complex with its Epitope Peptide
Mader, Pavel; Štouračová, Renata; Brynda, Jiří; Závada, Jan
2004 - English
CA IX is a cell surface protein that exerts capacity of binding cell surface receptors, and is associated with several types of human carcinomas. Monoclonal antibody M75 recognizes specifically an epitope identified as six repeats of amino acid sequence PGEEDLP, localized in the proteoglycan like domain of CA IX. Structural study of the M75 Fab fragment in complex with its epitope peptide PGEEDLPGEEDL is presented.
Keywords:
CAIX; antibody; structure
Available at various institutes of the ASCR
Structural Studies of anti-CA IX Monoclonal Antibody M75 Fab Fragment in Complex with its Epitope Peptide
CA IX is a cell surface protein that exerts capacity of binding cell surface receptors, and is associated with several types of human carcinomas. Monoclonal antibody M75 recognizes specifically an ...
A Phenylnorstatine Inhibitor Binding to HIV-1 Protease: Geometry, Protonation and Subsite-Pocket Interactions Analyzed at Atomic Resolution
Brynda, Jiří; Řezáčová, Pavlína; Fábry, Milan; Hořejší, Magdalena; Štouračová, Renata; Sedláček, Juraj; Souček, Milan; Hradilek, Martin; Lepšík, Martin; Konvalinka, Jan
2004 - English
The x-ray structure of a complex of HIV-1 protease (PR) with a phenylnorstatine inhibitor Z-Pns-Phe-Glu-Glu-NH2 has been determined at 1.03 A, the highest resolution so far reported for any HIV PR complex. The inhibiot shows subnanomolar Ki values for both the wild-type PR and the variant representing one of the most common mutations linked to resistance development. The structure displays a unique pattern of hydrogen bonding to the two catalytic aspartate residues. The high resolution permints to assess the donor/acceptor relations of this hydrogen bonding and to indicate a proton shared by the two catalytic residues. Structural mechanism for the unimpaired inhibition of the protease Val82Ala mutant is also suggested, based on energy calculations and analyses Byla určena rentgenová struktura komplexu proteázy HIV-1 (PR) s fenylnorstatinovým inhibitorem Z-Pns-Phe-Glu-Glu-NH(2) při rozlišení 1,03 A, nejvyšším rozlišení, které bylo doposud uvedeno pro kterýkoli komplex HIV PR. Tento inhibitor vykazuje subnanomolární hodnoty K(i) jak pro PR divokého typu, tak pro variantu představující jednu z nejběžnějších mutací spojenou s vývojem rezistence. Struktura obsahující fenylnorstatinovou část chirality (2R,3S) vykazuje jedinečný obraz vazby vodíkových můstků na dva katalytické aspartátové zbytky. Toto vysoké rozlišení umožňuje zjistit donorové a akceptorové vztahy těchto vodíkových můstků a určit proton sdílený oběma katalytickými zbytky. Je také navržen strukturální mechanismus nenarušené inhibice mutanty proteázy Val82Ala založený na energetických výpočtech a analýzách
Keywords:
inhibitor; HIV protease; atomic resolution
Available at various institutes of the ASCR
A Phenylnorstatine Inhibitor Binding to HIV-1 Protease: Geometry, Protonation and Subsite-Pocket Interactions Analyzed at Atomic Resolution
The x-ray structure of a complex of HIV-1 protease (PR) with a phenylnorstatine inhibitor Z-Pns-Phe-Glu-Glu-NH2 has been determined at 1.03 A, the highest resolution so far reported for any HIV PR ...
Characterization of the clones derived from the HT29 colorectal cancer cell line
Šloncová, Eva; Kučerová, Dana; Vojtěchová, Martina; Turečková, Jolana; Tuháčková, Zdena; Sovová, Vlasta
2004 - English
During our analysis of HT29 cell line we established several clones, which differed in the expression of some molecular markers, e.g. phosphorylation of Src protein, PKCbeta2 activity, CEA-CAM1 expression and in the response to the differentiating effect of sodium butyrate, indicated by activity of alkaline phosphatase Během analýzy buněčné linie HT29 jsme připravili několik stabilních klonů, které se lišily expresí některých molekulárních markerů, např. fosforylací proteinu Src, aktivitou PKCbeta2, expresí CEA-CAM1 a odpovědí na diferenciační účinek butyrátu sodného
Keywords:
colorectal carcinoma cells; differentiation
Available at various institutes of the ASCR
Characterization of the clones derived from the HT29 colorectal cancer cell line
During our analysis of HT29 cell line we established several clones, which differed in the expression of some molecular markers, e.g. phosphorylation of Src protein, PKCbeta2 activity, CEA-CAM1 ...
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