Number of found documents: 501
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Enzymatic detoxification of cyanide using cyanide hydratases
Sedova, Anastasia; Rucká, Lenka; Glozlová, Michaela; Novotný, Petr; Martínková, Ludmila; Bojarová, Pavla
2021 - English
Cyanide, a strong chelator of metals in vital enzymes and proteins, is very toxic for most living organisms. Wastewaters from the mining, metallurgical and chemical industries contain significant concentrations of free cyanide. Though it can be largely eliminated by physicochemical methods, these processes may in turn lead to the formation of other waste. In addition, no effective methods have been found to neutralize cyanide spills coming from industrial accidents. The use of enzymes to remove cyanide is an attractive alternative, which is environmentally friendly and applicable to high cyanide concentrations. Cyanide hydratases (CynHs) are of considerable interest for the decomposition of hazardous cyanide wastes. Here we investigate the biochemical properties of new CynHs from Stereum hirsutum and Exidia glandulosa, it is of fundamental importance to preserve the enzyme activity at alkaline pH as cyanide waste is stored under these conditions. Keywords: free cyanide; cyanide hydratase; cyanide spills; cyanide waste Available at various institutes of the ASCR
Enzymatic detoxification of cyanide using cyanide hydratases

Cyanide, a strong chelator of metals in vital enzymes and proteins, is very toxic for most living organisms. Wastewaters from the mining, metallurgical and chemical industries contain significant ...

Sedova, Anastasia; Rucká, Lenka; Glozlová, Michaela; Novotný, Petr; Martínková, Ludmila; Bojarová, Pavla
Mikrobiologický ústav, 2021

Purification of water soluble bioactive polysaccharides from biomass of heterotrophic mutant alga Chlorella vulgaris G11.
Sushytskyi, L.; Lukáč, Pavol; Bleha, R.; Čopíková, J.; Vannucci, Luca; Sinica, A.; Kaštánek, P.
2019 - English
Water extract obtained from biomass of mutant alga Chlorella vulgaris G11 was deproteinized and then purified with AEC on DEAE Sepharose FF and SEC on Sepaphadex G-75 superfine columns. Two high MW (>1000 kDa and 98,3 kDa) polysaccharides (CPS1 and CPS3) were eluted with water and 0.5M NaCl on DEAE column, and heteropolysaccharide with MW of 15 kDa (CPS2) was also eluted with water on DEAE. The former fractions were identified as branched rhamnans, the latter one as branched xylogalactofuranan of unusual structure. CPS1 shown properties of unstable polimer: according to GPC analysis and by using light scattering and RI detectors it was found, that some fraction of polymers (260-800 kDa) have self-assembling properties in aqueous solution, and MW of this fraction decreased during some time while polimer with initially bigger MW (>1000 kDa) became bigger. CPS3 was eluted with 0.3-0.5 M of NaCl on DEAE Sepharose FF column and have MW of 98,3 kDa. Cell toxicity of the products were tested in vitro on cell cultures for different concentrations Keywords: Mutant alga; Chlorella vulgaris G11; chromatography; polymer; cell cultures Available at various institutes of the ASCR
Purification of water soluble bioactive polysaccharides from biomass of heterotrophic mutant alga Chlorella vulgaris G11.

Water extract obtained from biomass of mutant alga Chlorella vulgaris G11 was deproteinized and then purified with AEC on DEAE Sepharose FF and SEC on Sepaphadex G-75 superfine columns. Two high MW ...

Sushytskyi, L.; Lukáč, Pavol; Bleha, R.; Čopíková, J.; Vannucci, Luca; Sinica, A.; Kaštánek, P.
Mikrobiologický ústav, 2019

Název práce v českém jazyce\nVývin črevného mikrobiómu – jeho preventívna a terapeutická funkcia. \n
Kajaba, I.; Kajabová, A.; Genčíková, M.; Genčík, B.; Lovászová, N.; Bencko, V.; Turek, B.; Šíma, Petr; Ševčík, J.
2019 - Czech
V naší studii jsme zkoumali střevní mikrobiom, jeho preventivní a terapeutickou funkci In our study we examined the intestinal microbiome, its preventive and therapeutic function. Keywords: Intestinal microbiome; immunity system; fecal transpalntation; dysbiosis Available at various institutes of the ASCR
Název práce v českém jazyce\nVývin črevného mikrobiómu – jeho preventívna a terapeutická funkcia. \n

V naší studii jsme zkoumali střevní mikrobiom, jeho preventivní a terapeutickou funkci


In our study we examined the intestinal microbiome, its preventive and therapeutic function.

Kajaba, I.; Kajabová, A.; Genčíková, M.; Genčík, B.; Lovászová, N.; Bencko, V.; Turek, B.; Šíma, Petr; Ševčík, J.
Mikrobiologický ústav, 2019

Důležitost mykologických průzkumů-popis nejčastějších dřevokazných hub a zkušenosti ze znalecké praxe
Gabriel, Jiří
2019 - Czech
The paper deals with the threat of buildings and timber structures with wood decaying fungi. It describes the most common wood decaying fungi occurring in interiors, their emergence and the spread of fungi in the room. Keywords: Wood Decaying Fungi; Wood Decaying Fungi Available at various institutes of the ASCR
Důležitost mykologických průzkumů-popis nejčastějších dřevokazných hub a zkušenosti ze znalecké praxe

The paper deals with the threat of buildings and timber structures with wood decaying fungi. It describes the most common wood decaying fungi occurring in interiors, their emergence and the spread of ...

Gabriel, Jiří
Mikrobiologický ústav, 2019

Význam skladby a aktivity mikrobiomu na homeostázu organismu (udržení zdraví)
Turek, B.; Šíma, Petr; Ševčík, J.; Kajaba, I.
2019 - Czech
Our study deals with the intestinal microbiome and its direct and indirect impact on overall health. Naše studie se zabývá střevním mikrobiomem a jeho přímým a nepřímým vlivem na celkové zdraví. Keywords: Intestinal microbiome; microbes; immunity system Available at various institutes of the ASCR
Význam skladby a aktivity mikrobiomu na homeostázu organismu (udržení zdraví)

Our study deals with the intestinal microbiome and its direct and indirect impact on overall health.


Naše studie se zabývá střevním mikrobiomem a jeho přímým a nepřímým vlivem na celkové zdraví.

Turek, B.; Šíma, Petr; Ševčík, J.; Kajaba, I.
Mikrobiologický ústav, 2019

Nanofiber dressing consisting of antisense rna-functionalized nanodiamonds for therapy of non-healing wounds in diabetic individuals
Neuhoferová, Eva; Petráková, V.; Vocetková, K.; Kindermann, Marek; Křivohlavá, Romana; Benson, Veronika
2018 - English
Non-healing wounds are serious complication in diabetic patients and represent an attractive challenge for development of suitable carrier system possessing constant and localized release of therapeutic biomolecule into the wound without any undesired side effects. Given the fact that these non-healing wounds are result of impaired balance in metalloproteinases synthesized by immune cells residing the wounds, gene therapy offering knock down of such enzymes is of great interest. \nHere we challenged a development of functional and biocompatible wound dressing enabling controlled release of trackable carrier loaded with therapeutic siRNA. Our dressing consists of scaffold from degradable polymer nanofibers enriched with fluorescent nanodiamond particles (FND). We have previously shown the nanodiamond particles are great carriers for antisense RNAs. Their advantages represent high biocompatibility, stable luminescence giving us the possibility to track the carrier system in the wound, and effective release of antisense RNA in the wound. Embedding of nanodiamond-siRNA systems into nanofiber scaffold enables continuous release of siRNA and maintaining the stable siRNA concentration in the wound site resulting in a promotion of wound healing. \nWe developed FND-siRNA complexes specific to MMP-9 that efficiently inhibit the expression of target MMP-9 mRNA. The complexes were embedded into core/shell nanofibers from PVA and PCL, visualized by confocal microscopy, and characterized by electron microscopy. Real-time PCR was used to assess the silencing effect of siRNA that has been delivered to target murine fibroblasts by FND released from nanofiber dressing. Nanofiber system with embedded FNDs was applied on wounds in diabetic animal models to evaluate its suitability regarding short and long term toxicity, efficacy, and handling in vivo. \n Keywords: Electrospun nanofibers; fluorescent nanodiamonds; RNA interference Available on request at various institutes of the ASCR
Nanofiber dressing consisting of antisense rna-functionalized nanodiamonds for therapy of non-healing wounds in diabetic individuals

Non-healing wounds are serious complication in diabetic patients and represent an attractive challenge for development of suitable carrier system possessing constant and localized release of ...

Neuhoferová, Eva; Petráková, V.; Vocetková, K.; Kindermann, Marek; Křivohlavá, Romana; Benson, Veronika
Mikrobiologický ústav, 2018

BETA-GLUCANS: WHAT APPLICATIONS? A GENERAL OVERVIEW
Vannucci, Luca; Mishra, Rajbardhan; Lukáč, Pavol; Tenti, Paolo; Rajsiglová, Lenka; Větvička, Václav; Šíma, Petr
2018 - English
Glucans are polysaccharides derived from various sources (e.g. mushrooms, algae, yeast, barley). They can target immune receptors, like Dectin-1 expressed on dendritic cells - antigen presenting cells. Otherwise, interest is rising for their use not only for modulation of immunity, but also of cholesterol levels, or as possible vaccine adjuvants, nanocarriers and functional food components. We will shortly review some aspects in human and veterinarian applications. Keywords: Antitumor immune-responses; growth-performance; gastrointestinal-tract Available at various institutes of the ASCR
BETA-GLUCANS: WHAT APPLICATIONS? A GENERAL OVERVIEW

Glucans are polysaccharides derived from various sources (e.g. mushrooms, algae, yeast, barley). They can target immune receptors, like Dectin-1 expressed on dendritic cells - antigen presenting ...

Vannucci, Luca; Mishra, Rajbardhan; Lukáč, Pavol; Tenti, Paolo; Rajsiglová, Lenka; Větvička, Václav; Šíma, Petr
Mikrobiologický ústav, 2018

BETA-GLUCANS: WHAT APPLICATIONS? A GENERAL OVERVIEW
Vannucci, Luca; Mishra, Rajbardhan; Lukáč, Pavol; Tenti, Paolo; Rajsiglová, Lenka; Větvička, Václav; Šíma, Petr
2018 - English
Glucans are polysaccharides derived from various sources (e.g. mushrooms, algae, yeast, barley). They can target immune receptors, like Dectin-1 expressed on dendritic cells - antigen presenting cells. Otherwise, interest is rising for their use not only for modulation of immunity, but also of cholesterol levels, or as possible vaccine adjuvants, nanocarriers and functional food components. We will shortly review some aspects in human and veterinarian applications. Keywords: antitumor immune-responses; growth-performance; gastrointestinal-tract Available at various institutes of the ASCR
BETA-GLUCANS: WHAT APPLICATIONS? A GENERAL OVERVIEW

Glucans are polysaccharides derived from various sources (e.g. mushrooms, algae, yeast, barley). They can target immune receptors, like Dectin-1 expressed on dendritic cells - antigen presenting ...

Vannucci, Luca; Mishra, Rajbardhan; Lukáč, Pavol; Tenti, Paolo; Rajsiglová, Lenka; Větvička, Václav; Šíma, Petr
Mikrobiologický ústav, 2018

Beta-Glucans: What Applications? A General Overview
Vannucci, Luca; Mishra, Rajbardhan; Lukáč, Pavol; Tenti, Paolo; Rajsiglová, Lenka; Větvička, V.; Šíma, Petr
2018 - English
Glucans are polysaccharides derived from various sources (e.g. mushrooms, algae, yeast, barley). They can target immune receptors, like Dectin-1 expressed on dendritic cells - antigen presenting cells. Otherwise, interest is rising for their use not only for modulation of immunity, but also of cholesterol levels, or as possible vaccine adjuvants, nanocarriers and functional food components. We will shortly review some aspects in human and veterinarian applications. Keywords: antitumor immune-responses; growth-performance; gastrointestinal-tract Available at various institutes of the ASCR
Beta-Glucans: What Applications? A General Overview

Glucans are polysaccharides derived from various sources (e.g. mushrooms, algae, yeast, barley). They can target immune receptors, like Dectin-1 expressed on dendritic cells - antigen presenting ...

Vannucci, Luca; Mishra, Rajbardhan; Lukáč, Pavol; Tenti, Paolo; Rajsiglová, Lenka; Větvička, V.; Šíma, Petr
Mikrobiologický ústav, 2018

Biodegradace aktivních farmaceutických látek termotolerantním biokatalyzátorem
Palyzová, Andrea; Marešová, Helena
2018 - Czech
Charakterizace růstových vlastností kmenů/směsné kultury mikroorganismů v minerálním mediu při vyšších teplotách. Stanovení biodegradačního potenciálu termotolerantních kmenů/směsné kultury pro residuální koncentrace farmak v prostředí. \n\n Characterization of growth parameters of strains / mixed cultures of microorganisms in mineral medium at higher temperatures. Determination of biodegradation potential of thermotolerant strains / mixed cultures for residual drug concentrations in the environment Keywords: degradation; pharmaceutical substance; thermotolorant Available at various institutes of the ASCR
Biodegradace aktivních farmaceutických látek termotolerantním biokatalyzátorem

Charakterizace růstových vlastností kmenů/směsné kultury mikroorganismů v minerálním mediu při vyšších teplotách. Stanovení biodegradačního potenciálu termotolerantních kmenů/směsné kultury pro ...

Palyzová, Andrea; Marešová, Helena
Mikrobiologický ústav, 2018

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