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Cross-border Cooperation in Czech-German Non-profit Organizations
KUTHANOVÁ, Kateřina
2019 - English
This thesis examines Czech-German cross-border cooperation within intercultural communication, identifies problems and develops possible solutions. The thesis is divided into a practical and a theoretical part. The theoretical part first describes the Czech-German cross-border cooperation and then intercultural communication, followed by a discussion of the context and the research methods. In the practical part, the author first performs a sequential analysis of data collected via interviews conducted with people working in the Tandem organization. The results of the analysis are interpreted, problems within the cooperation of Tandem are identified, and possible solutions developed. A glossary divided into two parts, recapitulating the definitions of relevant terms from the first two theoretical chapters of this thesis, is listed at the end of this work. A French résumé summarizing all the important points and conclusions is enclosed with the thesis. The aim of this thesis is to answer the following research question: "Linguistic and intercultural challenges of Czech-German non-profit cross-border cooperation: The case of the Tandem organization." This thesis examines Czech-German cross-border cooperation within intercultural communication, identifies problems and develops possible solutions. The thesis is divided into a practical and a theoretical part. The theoretical part first describes the Czech-German cross-border cooperation and then intercultural communication, followed by a discussion of the context and the research methods. In the practical part, the author first performs a sequential analysis of data collected via interviews conducted with people working in the Tandem organization. The results of the analysis are interpreted, problems within the cooperation of Tandem are identified, and possible solutions developed. A glossary divided into two parts, recapitulating the definitions of relevant terms from the first two theoretical chapters of this thesis, is listed at the end of this work. A French résumé summarizing all the important points and conclusions is enclosed with the thesis. The aim of this thesis is to answer the following research question: "Linguistic and intercultural challenges of Czech-German non-profit cross-border cooperation: The case of the Tandem organization." Keywords: Cross-border cooperation; non-profit organizations; intercultural communication Available in the Digital Repository of University of South Bohemia.
Cross-border Cooperation in Czech-German Non-profit Organizations

This thesis examines Czech-German cross-border cooperation within intercultural communication, identifies problems and develops possible solutions. The thesis is divided into a practical and a ...

KUTHANOVÁ, Kateřina
Jihočeská univerzita v Českých Budějovicích, 2019

Testing cross-amplification of microsatellites on European Ranunculus sect. Batrachium species
GEMEINHARDT, Helene Sabine
2019 - English
Cross-amplification of microsatellites on European Ranunculus sect. Batrachium species was tested using primers that were designed for R. bungei. The main aim was to test whether these primers anneal successfully and the regions can be amplified using PCR in order to use them for population genetical studies. Cross-amplification of microsatellites on European Ranunculus sect. Batrachium species was tested using primers that were designed for R. bungei. The main aim was to test whether these primers anneal successfully and the regions can be amplified using PCR in order to use them for population genetical studies. Available in the Digital Repository of University of South Bohemia.
Testing cross-amplification of microsatellites on European Ranunculus sect. Batrachium species

Cross-amplification of microsatellites on European Ranunculus sect. Batrachium species was tested using primers that were designed for R. bungei. The main aim was to test whether these primers anneal ...

GEMEINHARDT, Helene Sabine
Jihočeská univerzita v Českých Budějovicích, 2019

The interaction of Streptomyces-like bacteria and model microorganisms in secondary metabolite production, motility and hemolytic activities - Experimental
WURZER, Theresa
2019 - English
In this thesis, environmental and clinical Streptomyces strains were used to test the hypothesis if isolates from different isolation sources vary in frequency of beta-hemolytic activity. Moreover, their interaction with two human pathogens Pseudomonas aeruginosa and Staphylococcus aureus was tested in parallel and T-type co-cultivations. For this, the responses (beta-hemolytic activity, growth, etc.) of both streptomycetes as well as model pathogens were evaluated. Furthermore, the frequency of observed interaction patterns with tested pathogens were compared between environmental and clinical Streptomyces strains as such study is lacking. Keywords: Streptomycetes; microbial interactions; secondary metabolites; hemolysis Available in the Digital Repository of University of South Bohemia.
The interaction of Streptomyces-like bacteria and model microorganisms in secondary metabolite production, motility and hemolytic activities - Experimental

In this thesis, environmental and clinical Streptomyces strains were used to test the hypothesis if isolates from different isolation sources vary in frequency of beta-hemolytic activity. Moreover, ...

WURZER, Theresa
Jihočeská univerzita v Českých Budějovicích, 2019

Expression and purification of recombinant Sericin-2 and Salivary gland secretion 3
VU, Trang Thanh
2019 - English
Recombinant proteins derived from Bombyx mori Ser-2 gene and Drosophila melanogaster Sgs3 gene were expressed in bacterial expression systems, purified and analyzed using different molecular biology techniques. The pure proteins were tested for cell adhesive properties by coating hydrophobic surfaces with the proteins and monitoring the growth of adherent cells in vitro. By showing adequate expression and resultant yield, as well as the ability to aid cell adhesion, the adhesive proteins present great potential for future biomaterials. Recombinant proteins derived from Bombyx mori Ser-2 gene and Drosophila melanogaster Sgs3 gene were expressed in bacterial expression systems, purified and analyzed using different molecular biology techniques. The pure proteins were tested for cell adhesive properties by coating hydrophobic surfaces with the proteins and monitoring the growth of adherent cells in vitro. By showing adequate expression and resultant yield, as well as the ability to aid cell adhesion, the adhesive proteins present great potential for future biomaterials. Keywords: proteins; recombinant; expression; bombyx; drosophila Available in the Digital Repository of University of South Bohemia.
Expression and purification of recombinant Sericin-2 and Salivary gland secretion 3

Recombinant proteins derived from Bombyx mori Ser-2 gene and Drosophila melanogaster Sgs3 gene were expressed in bacterial expression systems, purified and analyzed using different molecular biology ...

VU, Trang Thanh
Jihočeská univerzita v Českých Budějovicích, 2019

Functional characterization of succinyl-CoA synthetase in the bloodstream form of Trypanosoma brucei
KUBIŠOVÁ, Karolína
2019 - English
During the infectious bloodstream stage (BF), the human pathogen Trypanosoma brucei lacks a canonical cytochrome-mediated respiratory chain. Therefore, this form of the parasite depends on the reverse activity of the FoF1-ATP synthase to hydrolyze ATP and pump protons into the mitochondrial intermembrane space to maintain the essential membrane potential. The dogma also states that the sole source of organellar ATP is the mitochondrial ADP/ATP carrier, which imports glycolytically derived ATP from the cytosol. In this study, we explore the possibility that the BF mitochondrion is able to contribute to its own pool of consumable ATP by utilizing an active succinyl-CoA synthase to generate ATP via substrate phosphorylation. During the infectious bloodstream stage (BF), the human pathogen Trypanosoma brucei lacks a canonical cytochrome-mediated respiratory chain. Therefore, this form of the parasite depends on the reverse activity of the FoF1-ATP synthase to hydrolyze ATP and pump protons into the mitochondrial intermembrane space to maintain the essential membrane potential. The dogma also states that the sole source of organellar ATP is the mitochondrial ADP/ATP carrier, which imports glycolytically derived ATP from the cytosol. In this study, we explore the possibility that the BF mitochondrion is able to contribute to its own pool of consumable ATP by utilizing an active succinyl-CoA synthase to generate ATP via substrate phosphorylation. Available in the Digital Repository of University of South Bohemia.
Functional characterization of succinyl-CoA synthetase in the bloodstream form of Trypanosoma brucei

During the infectious bloodstream stage (BF), the human pathogen Trypanosoma brucei lacks a canonical cytochrome-mediated respiratory chain. Therefore, this form of the parasite depends on the reverse ...

KUBIŠOVÁ, Karolína
Jihočeská univerzita v Českých Budějovicích, 2019

Trehalose as an important energy source for immune cells in Drosophila melanogaster
LEHR, Katharina
2019 - English
The aim of this thesis was the identification of the change of trehalase gene expression in hemocytes of Drosophila melanogaster in uninfected and infected larvae using parasitoid wasp infection by Leptopilina boulardi. Moreover, the energy supply was shut down by deactivating the trehalose transporter and measuring the lamellocyte production, again in uninfected and infected larva to test the role of trehalose for the selfish immune system. The aim of this thesis was the identification of the change of trehalase gene expression in hemocytes of Drosophila melanogaster in uninfected and infected larvae using parasitoid wasp infection by Leptopilina boulardi. Moreover, the energy supply was shut down by deactivating the trehalose transporter and measuring the lamellocyte production, again in uninfected and infected larva to test the role of trehalose for the selfish immune system. Available in the Digital Repository of University of South Bohemia.
Trehalose as an important energy source for immune cells in Drosophila melanogaster

The aim of this thesis was the identification of the change of trehalase gene expression in hemocytes of Drosophila melanogaster in uninfected and infected larvae using parasitoid wasp infection by ...

LEHR, Katharina
Jihočeská univerzita v Českých Budějovicích, 2019

Genome analysis of Kutzneria sp. strain BCCO 10_1627 and detection of antifungal secondary metabolites
JERNEJ, Linda
2019 - English
Secondary metabolites, produced by various microbes, are used since decades for treatment of diseases. The bacterial genus Kutzneria is known for synthesizing compounds with antifungal activity, which might be used against pathogenic fungi, causing agricultural relevant diseases. This thesis deals with the genome analysis of the strain Kutzneria sp. BCCO 10_1627 for prediction of secondary metabolite gene cluster. Moreover, the antifungal activity of the strain was determined experimentally. Secondary metabolites, produced by various microbes, are used since decades for treatment of diseases. The bacterial genus Kutzneria is known for synthesizing compounds with antifungal activity, which might be used against pathogenic fungi, causing agricultural relevant diseases. This thesis deals with the genome analysis of the strain Kutzneria sp. BCCO 10_1627 for prediction of secondary metabolite gene cluster. Moreover, the antifungal activity of the strain was determined experimentally. Available in the Digital Repository of University of South Bohemia.
Genome analysis of Kutzneria sp. strain BCCO 10_1627 and detection of antifungal secondary metabolites

Secondary metabolites, produced by various microbes, are used since decades for treatment of diseases. The bacterial genus Kutzneria is known for synthesizing compounds with antifungal activity, which ...

JERNEJ, Linda
Jihočeská univerzita v Českých Budějovicích, 2019

Characterization of the recombinant proteins SER 3 and BARN
KARL, Lorenz
2019 - English
This thesis shows the process of expressing two promising recombinant proteins in a bacterial expression system and testing the adhesive properties of the pure properties in a cell culture experiment. Moreover, this thesis presents an overview of several application possibilities for bioadhesive materials. This thesis shows the process of expressing two promising recombinant proteins in a bacterial expression system and testing the adhesive properties of the pure properties in a cell culture experiment. Moreover, this thesis presents an overview of several application possibilities for bioadhesive materials. Available in the Digital Repository of University of South Bohemia.
Characterization of the recombinant proteins SER 3 and BARN

This thesis shows the process of expressing two promising recombinant proteins in a bacterial expression system and testing the adhesive properties of the pure properties in a cell culture ...

KARL, Lorenz
Jihočeská univerzita v Českých Budějovicích, 2019

Sialic acid as a recognition motif for host-originated glycoproteins in Ixodes ricinus
SAMEK, Jakob
2019 - English
Glycans are found on the surface of every living cell and they are responsible for a countless number of functions. N-acetylneuraminic acid (often called sialic acid) is mostly found in vertebrate and only to a small degree in arthropods. The aim of this thesis was to find out if sialic acid is a recognition motif for the tick organism to take up and incorporate or recycle host originated proteins. Keywords: sialic acid; Ixodes ricinus; IRE/CTMV 19; glycoprotein Available in the Digital Repository of University of South Bohemia.
Sialic acid as a recognition motif for host-originated glycoproteins in Ixodes ricinus

Glycans are found on the surface of every living cell and they are responsible for a countless number of functions. N-acetylneuraminic acid (often called sialic acid) is mostly found in vertebrate and ...

SAMEK, Jakob
Jihočeská univerzita v Českých Budějovicích, 2019

Comparison of language teaching methods
KUČEROVÁ, Alžběta
2019 - English
Tato bakalářská práce se zabývá vyučovacími metodami a tím jako jsou aplikovány v praxi. Zaměřuje se především na jejich vývoj a popisuje techniky a principy, které jsou pro ně charakteristické. Praktická část vychází z poznatků získaných při pozorování hodin v jazykových školách a zkušenosti jedné účastnice, která absolvovala sugestopedický kurz. V každém kurzu jsou pozorovány stejné aspekty, které hrají důležitou roli, co se týče úspěšnosti přístupu ve vyučování. Tato práce si klade za cíl poukázat na silné a slabé stránky jednotlivých metod a porovnat výsledky z pozorovacích hodin. Abstract This thesis is concerned with language teaching methods and how they there are applied in practise. It focuses mainly on their development and description of techniques and principles that are characteristic for these methods. The practical part is patterned on findings gained from observation of classes in different language schools and also on experience of one student who attended the suggestopedic course. In each course the same aspects are observed, which play an important role in a success rate of an approach to teaching. The thesis sets out to show the advantages and disadvantages of particular methods and compared the results of classroom observation. Keywords: vyučování; metody; jazyková škola; techniky a principy Available in the Digital Repository of University of South Bohemia.
Comparison of language teaching methods

Tato bakalářská práce se zabývá vyučovacími metodami a tím jako jsou aplikovány v praxi. Zaměřuje se především na jejich vývoj a popisuje techniky a principy, které jsou pro ně charakteristické. ...

KUČEROVÁ, Alžběta
Jihočeská univerzita v Českých Budějovicích, 2019

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