Number of found documents: 351
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A novel structurally characterized haloacid dehalogenase superfamily phosphatase from Thermococcus thioreducens with diverse substrate specificity
HAVLÍČKOVÁ, Petra
2024 - English
This RNDr. thesis is focused on complex studies of a haloacid dehalogenase superfamily phosphatase member with an unknown substrate specificity, isolated from a hyperthermofilic archeon Thermococcus thioreducens. The HAD phosphatase, internally named as Tt82, was isolated, expressed and purified using standard techniques and the crystal structure was solved by X-ray crystallography method. The refined crystal structure displayed characteristic HAD superfamily elements, such as the HAD canonical fold with the HAD signature motifs in the active site. Further, possible substrates for the enzyme were proposed and examined by computational docking in the active site of the HAD phosphatase. The results of the docking studies served as a basis for an experimental measurement of Tt82 enzymatic activity against selected substrates at two different temperatures. HAD phosphatase Tt82 is a promising target for the biotechnological field, mainly for its wide substrate specificity and higher temperature optima. Available in the Digital Repository of University of South Bohemia.
A novel structurally characterized haloacid dehalogenase superfamily phosphatase from Thermococcus thioreducens with diverse substrate specificity

This RNDr. thesis is focused on complex studies of a haloacid dehalogenase superfamily phosphatase member with an unknown substrate specificity, isolated from a hyperthermofilic archeon Thermococcus ...

HAVLÍČKOVÁ, Petra
Jihočeská univerzita v Českých Budějovicích, 2024

Functional characterization of the insulin signaling pathway in the hard tick Ixodes ricinus
KOZELKOVÁ, Tereza
2024 - English
In this thesis, the molecular and functional characterization of the insulin receptor signaling pathway (ISP) in hard tick Ixodes ricinus was characterized. As obligatory blood-feeding ectoparasites, ticks play a crucial role in the transmission of various pathogens, including bacteria, viruses, and protozoa, which has a significant impact on human and animal health. The parasite is strictly bonded with its host through a unidirectional transmission of nutrition for its survival, development, and reproduction. The ISP, a highly conserved system, regulates numerous physiological and anabolic processes to nutritional availability. This study aims to investigate the functionality of key components of the ISP pathway identified in the midgut transcriptome, namely the insulin receptor (IrInR), the protein kinase B called AKT (IrAKT), and the target of rapamycin (IrTOR). To achieve this goal, the expression profiles of these components in tick tissues during feeding and after detachment were investigated using qRT-PCR. Additionally, RNAi silencing of individual components was performed, and the phenotype of the ticks was observed. To further clarify the effects of ISP, immunization of rabbits with recombinant IrInR protein and tick infestation were investigated. Available in the Digital Repository of University of South Bohemia.
Functional characterization of the insulin signaling pathway in the hard tick Ixodes ricinus

In this thesis, the molecular and functional characterization of the insulin receptor signaling pathway (ISP) in hard tick Ixodes ricinus was characterized. As obligatory blood-feeding ...

KOZELKOVÁ, Tereza
Jihočeská univerzita v Českých Budějovicích, 2024

Red-backed shrike (Lanius collurio) versus common cuckoo (Cuculus canorus): An example of ineffective cuckoo-hawk mimicry
KRAUSOVÁ, Ladislava
2023 - English
V této práci jsem testovala reakci ťuhýka obecného (Lanius collurio) na hnízdního parazita, kukačku obecnou (Cuculus canorus) (šedá forma), predátora, krahujce obecného (Accipiter nisus) a neškodnou kontrolu, hrdličku divokou (Streptoptelia turtur). Prokázala jsem, že ťuhýci od sebe dokážou rozlišit jednotlivé atrapy a adekvátně na ně reagovat. Navíc jsem prokázala, že imitace krahujce kukačkou je u ťuhýků neúspěšná. In this work, I tested the reaction of the red-backed shrike (Lanius collurio) against a nest parasite, the common cuckoo (Common cuckoo) (grey form), predator, Eurasian sparrowhawk (Accipiter nisus) and harmless intruder, turtle dove (Streptopelia turtur). I proved that shrikes can differ between presented stimuli and adequately respond to them. Moreover, I proved that the cuckoo-hawk mimicry is not successful against shrikes. Keywords: ťuhýk obecný; cuckoo-hawk mimicry; hnízdní parazitismus; obrana hnízda Available in the Digital Repository of University of South Bohemia.
Red-backed shrike (Lanius collurio) versus common cuckoo (Cuculus canorus): An example of ineffective cuckoo-hawk mimicry

V této práci jsem testovala reakci ťuhýka obecného (Lanius collurio) na hnízdního parazita, kukačku obecnou (Cuculus canorus) (šedá forma), predátora, krahujce obecného (Accipiter nisus) a neškodnou ...

KRAUSOVÁ, Ladislava
Jihočeská univerzita v Českých Budějovicích, 2023

Nine-year bird community development on Radovesická spoil heap: impacts of restoration approach and vegetation characteristics
KOREJS, Kryštof
2023 - English
Worldwide environmental effects of anthropogenic land modification have made apparent the urgent need of ecological restoration. However, the methods employed in restoration of post-industrial sites vary across different regions. This thesis aims to examine the outcomes of different restoration approaches by examining avian communities inhabiting a vast spoil heap in Central Europe. Insights into the effects of different types of management and associated vegetation characteristics can guide active restoration of post-industrial sites. Worldwide environmental effects of anthropogenic land modification have made apparent the urgent need of ecological restoration. However, the methods employed in restoration of post-industrial sites vary across different regions. This thesis aims to examine the outcomes of different restoration approaches by examining avian communities inhabiting a vast spoil heap in Central Europe. Insights into the effects of different types of management and associated vegetation characteristics can guide active restoration of post-industrial sites. Keywords: ecological restoration; revegetation; agriculture; bird diversity; tree planting; bird community Available in the Digital Repository of University of South Bohemia.
Nine-year bird community development on Radovesická spoil heap: impacts of restoration approach and vegetation characteristics

Worldwide environmental effects of anthropogenic land modification have made apparent the urgent need of ecological restoration. However, the methods employed in restoration of post-industrial sites ...

KOREJS, Kryštof
Jihočeská univerzita v Českých Budějovicích, 2023

Tasty rewards for ants: differences in elaiosome and seed metabolite profiles are consistent across species and reflect taxonomic relatedness
KONEČNÁ, Marie
2023 - English
The reward for ants in myrmecochory is a nutrient-rich appendage, the elaiosome. This study evaluates the metabolite composition of five groups (i.e. amino acids, fatty acids, organic acids, polyols, and sugars) in elaiosomes compared to the respective seeds. Two plant species from each of the four families (Amaryllidaceae, Boraginaceae, Papaveraceae, and Poaceae) were examined, with each species represented by three populations. The hierarchically designed data enabled the decomposition of variability in metabolite composition at three levels: family, species, and population. Another aspect studied was the consistency of elaiosome metabolite composition across unrelated species from monocotyledonous and dicotyledonous families with different elaiosome origins, which may reflect the convergent evolution of elaiosomes. The reward for ants in myrmecochory is a nutrient-rich appendage, the elaiosome. This study evaluates the metabolite composition of five groups (i.e. amino acids, fatty acids, organic acids, polyols, and sugars) in elaiosomes compared to the respective seeds. Two plant species from each of the four families (Amaryllidaceae, Boraginaceae, Papaveraceae, and Poaceae) were examined, with each species represented by three populations. The hierarchically designed data enabled the decomposition of variability in metabolite composition at three levels: family, species, and population. Another aspect studied was the consistency of elaiosome metabolite composition across unrelated species from monocotyledonous and dicotyledonous families with different elaiosome origins, which may reflect the convergent evolution of elaiosomes. Keywords: Myrmecochory; Convergent evolution; Variation partitioning; Ant-plant mutualism; Seed dispersal Available in the Digital Repository of University of South Bohemia.
Tasty rewards for ants: differences in elaiosome and seed metabolite profiles are consistent across species and reflect taxonomic relatedness

The reward for ants in myrmecochory is a nutrient-rich appendage, the elaiosome. This study evaluates the metabolite composition of five groups (i.e. amino acids, fatty acids, organic acids, polyols, ...

KONEČNÁ, Marie
Jihočeská univerzita v Českých Budějovicích, 2023

Mannan-BAM, TLR ligands, and anti-CD40 immunotherapy in established murine pancreatic adenocarcinoma: understanding therapeutic potentials and limitations
VENHAUEROVÁ, Anna
2023 - English
The aim of this RNDr. Thesis is focused on understanding therapeutic potentials and limitations of the antitumor MBTA immunotherapy which is based on synergy of TLR agonists, anti-CD40, and phagocytosis stimulating ligands anchored into the tumor cell membranes. In this study, immunotherapy was tested in murine pancreatic adenocarcinoma Panc02 model. Firstly, the short-term and long-term efficacy of MBTA therapy was tested using established subcutaneous Panc02 tumors two times larger than in previous study. Secondly, the work is devoted to better understanding of the adaptive immunity involvement focusing on CD4+ and CD8+ T lymphocytes during the therapy and their effect on tumor volume reduction, long-term survival and resistance against tumor rechallenge. Subsequently, the ability of immunological memory to cross over the blood-brain barrier confirming its potential applicability in metastatic brain tumors was examined. Moreover, the antigen specificity of the immunological memory was evaluated. Finally, the potential of MBTA therapy to cure metastatic disease, represented by bilateral Panc02 mouse model, was studied. In this case, the MBTA therapy manifested a lower therapeutic response. Therefore, it was combined with diverse therapeutic approaches, such as intratumoral application of anti-CTLA-4 antibody, heat-killed Listeria monocytogenes, chemoablation using EtOH, targeting the tumor microenvironment by hyaluronidase, simultaneous injections of MBTA therapy in primary and secondary distant tumors, and its combination with RT. Despite all these combinations, our results showed that only simultaneous application of MBTA therapy into both tumors has potential for the treatment of the bilateral Panc02. The aim of this RNDr. Thesis is focused on understanding therapeutic potentials and limitations of the antitumor MBTA immunotherapy which is based on synergy of TLR agonists, anti-CD40, and phagocytosis stimulating ligands anchored into the tumor cell membranes. In this study, immunotherapy was tested in murine pancreatic adenocarcinoma Panc02 model. Firstly, the short-term and long-term efficacy of MBTA therapy was tested using established subcutaneous Panc02 tumors two times larger than in previous study. Secondly, the work is devoted to better understanding of the adaptive immunity involvement focusing on CD4+ and CD8+ T lymphocytes during the therapy and their effect on tumor volume reduction, long-term survival and resistance against tumor rechallenge. Subsequently, the ability of immunological memory to cross over the blood-brain barrier confirming its potential applicability in metastatic brain tumors was examined. Moreover, the antigen specificity of the immunological memory was evaluated. Finally, the potential of MBTA therapy to cure metastatic disease, represented by bilateral Panc02 mouse model, was studied. In this case, the MBTA therapy manifested a lower therapeutic response. Therefore, it was combined with diverse therapeutic approaches, such as intratumoral application of anti-CTLA-4 antibody, heat-killed Listeria monocytogenes, chemoablation using EtOH, targeting the tumor microenvironment by hyaluronidase, simultaneous injections of MBTA therapy in primary and secondary distant tumors, and its combination with RT. Despite all these combinations, our results showed that only simultaneous application of MBTA therapy into both tumors has potential for the treatment of the bilateral Panc02. Keywords: cancer immunotherapy; immunology; cancer; pancreatic adenocarcinoma; TLR agonists; anti-CD40; phagocytosis Available in the Digital Repository of University of South Bohemia.
Mannan-BAM, TLR ligands, and anti-CD40 immunotherapy in established murine pancreatic adenocarcinoma: understanding therapeutic potentials and limitations

The aim of this RNDr. Thesis is focused on understanding therapeutic potentials and limitations of the antitumor MBTA immunotherapy which is based on synergy of TLR agonists, anti-CD40, and ...

VENHAUEROVÁ, Anna
Jihočeská univerzita v Českých Budějovicích, 2023

Determinants of Piper (Piperaceae) climber composition in a lowland tropical rainforest in New Guinea
LISNER, Aleš
2023 - English
The study examines the distribution of root climbers from the genus Piper on host trees depending on environmental properties and host tree characteristics in a lowland primary tropical rainforest in Papua New Guinea. More specifically, the study examines the relationship between the climbers' presence and various factors including topography (e.g. slope, altitude), vegetation properties, and host tree characteristics. The findings reveal that Piper climbers tend to avoid higher altitudes, steeper slopes, closed canopy layers, and areas with larger total basal area of host vegetation. The preferences of Piper climbers for certain tree species are primarily influenced by the properties of the host trees, particularly their diameter at breast height (DBH). Additionally, the study suggests that Piper species are more commonly found on rare tree species, possibly due to their preference for larger host tree DBH. The results highlight the non-random association between climbers and their host trees, which is influenced by local environmental conditions. These interactions are likely to have important implications for forest vegetation dynamics and the maintenance of diversity. Keywords: Piper; climber; tropical forest Available in the Digital Repository of University of South Bohemia.
Determinants of Piper (Piperaceae) climber composition in a lowland tropical rainforest in New Guinea

The study examines the distribution of root climbers from the genus Piper on host trees depending on environmental properties and host tree characteristics in a lowland primary tropical rainforest in ...

LISNER, Aleš
Jihočeská univerzita v Českých Budějovicích, 2023

Effects of hydropeaking on the attached eggs of a rheophilic cyprinid species
BARTOŇ, Daniel
2023 - English
Ability of eggs of asp (Leuciscus aspius) to survive an increased water velocity was tested in experimental condition as well as in situ. Hydropeaking occurs on the study site and can cause detachment of most spawned eggs. Keywords: fish reproduction; Leuciscus aspius; wildlife conservation; river ecology; habitat alternation Available in the Digital Repository of University of South Bohemia.
Effects of hydropeaking on the attached eggs of a rheophilic cyprinid species

Ability of eggs of asp (Leuciscus aspius) to survive an increased water velocity was tested in experimental condition as well as in situ. Hydropeaking occurs on the study site and can cause detachment ...

BARTOŇ, Daniel
Jihočeská univerzita v Českých Budějovicích, 2023

Prevalence střevního prvoka Dientamoeba fragilis ve zdravé populaci lidí a zvířat v České republice
KAŠPAROVÁ, Andrea
2023 - English
Dientamoeba fragilis je kosmopolitně rozšířený střevní prvok, přičemž prevalence závisí na studované kohortě lidí a použitých diagnostických metodách. Jeho role v lidském zdraví zůstává nejasná především kvůli velmi malému počtu studií zaměřených na populaci, která netrpí zažívacími obtížemi. Hlavním cílem této studie bylo rozšířit znalosti o epidemiologii prvoka D. fragilis u zdravých lidí a jejich zvířat v České republice. Dientamoeba fragilis is a cosmopolitan intestinal protist colonizing the human gut with varying prevalence depending on the cohort studied and the diagnostic methods used. Its role in human health remains unclear mainly due to the very sporadic number of cross-sectional studies in gut-healthy populations. The main objective of this study was to expand knowledge of the epidemiology of D. fragilis in gut-healthy humans and their animals in the Czech republic. Keywords: dientamoeba fragilis; prvok; prevalence; zdraví dobrovolníci; genotyp; domácí zvířata; molekulární metody Available in the Digital Repository of University of South Bohemia.
Prevalence střevního prvoka Dientamoeba fragilis ve zdravé populaci lidí a zvířat v České republice

Dientamoeba fragilis je kosmopolitně rozšířený střevní prvok, přičemž prevalence závisí na studované kohortě lidí a použitých diagnostických metodách. Jeho role v lidském zdraví zůstává nejasná ...

KAŠPAROVÁ, Andrea
Jihočeská univerzita v Českých Budějovicích, 2023

Experimental Infection of Mice and Ticks with the Human Isolate of Anaplasma phagocytophilum NY-18
KALINOVÁ, Eliška
2023 - English
Anaplasma phagocytophilum je významný patogen přenášený klíšťaty, který kolonizuje granulocytární buňky obratlovců. Vyskytuje se v Severní Americe a v několika evropských zemích. Způsobuje závažná onemocnění domácích zvířat a potenciálně smrtelné onemocnění lidí zvané lidská granulocytární anaplazmóza (HGA). Vzhledem k rostoucí prevalenci A. phagocytophilum je nutné zavedení zvířecích modelů. Tato studie se zabývá přenosem kmene bakterie A. phagocytophilum NY-18 dvěma druhy klíšťat (Ixodes ricinus a Ixodes scapularis) a zavádí laboratorní model přenosu A. phagocytophilum klíštětem I. ricinus u myší. Práce dále porovnává oba přenosy i dynamiku infekce. Anaplasma phagocytophilum is a significant tick-borne pathogen that colonizes granulocytic cells in vertebrate hosts. It is found in North America and in several European countries. It causes severe diseases in domestic animals and potentially fatal disease in humans called human granulocytic anaplasmosis (HGA). The implementation of animal models is necessary due to the increasing prevalence of A. phagocytophilum. This study addresses the transmission of the human isolate of the bacterium A. phagocytophilum NY-18 by two tick species (Ixodes ricinus and Ixodes scapularis) and implements a laboratory model for the transmission of A. phagocytophilum by the tick I. ricinus in mice. The thesis also compares both transmissions and infection dynamics. Keywords: laboratorní model; klíště; Ixodes ricinus; Ixodes scapularis; anaplazmóza; myši; HGA Available in the Digital Repository of University of South Bohemia.
Experimental Infection of Mice and Ticks with the Human Isolate of Anaplasma phagocytophilum NY-18

Anaplasma phagocytophilum je významný patogen přenášený klíšťaty, který kolonizuje granulocytární buňky obratlovců. Vyskytuje se v Severní Americe a v několika evropských zemích. Způsobuje závažná ...

KALINOVÁ, Eliška
Jihočeská univerzita v Českých Budějovicích, 2023

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