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Trapping of volatile cadmium species on gold surfaces for atomic absorption spectrometry
Sagapova, Linda; Svoboda, Milan; Matoušek, Tomáš; Kratzer, Jan
2022 - English
Simple preconcentration step was developed for cadmium determination after chemical vapor generation prior to atomic absorption spectrometric detection. Chemical vapor generation of cadmium was performed in the presence of Cr3+/KCN as modifiers.
Keywords:
chemical vapor generation; cadmium; atomic spectrometry
Available in digital repository of the ASCR
Trapping of volatile cadmium species on gold surfaces for atomic absorption spectrometry
Simple preconcentration step was developed for cadmium determination after chemical vapor generation prior to atomic absorption spectrometric detection. Chemical vapor generation of cadmium was ...
Biodostupnost prvků a oxidativní potenciál městského aerosolu
Cigánková, Hana; Mikuška, Pavel; Hegrová, J.
2022 - Czech
Atmosférické aerosoly (ParticulateMatter, PM) mají významný dopad na zdraví a životní prostředí. Schopnost aerosolů produkovat reaktivní formy kyslíku (ROS) je jedním z často navrhovaných mechanismů souvisejících s negativními účinky aerosolu na lidské zdraví. Oxidační stres, který je způsobený zvýšenou koncentrací ROS, je spojován s celou řadou kardiovaskulárních a respiračních onemocnění. Schopnost aerosolu produkovat ROS je nazývána oxidativní potenciál (OP). Přestože schopnost PM tvořit ROS je dobře korelována s hmotnostní koncentrací PM, může více\nzáviset na fyzikálně-chemických vlastnostech PM. Bylo prokázáno, že prvky vázané na PM významně přispívají k OP aerosolu. Expozice prvkům bývá spojena s několika zdravotními problémy (např. respirační a kardiovaskulární onemocnění, astma, zánětlivé procesy, rakovina plic). Atmospheric aerosols (Particulate Matter, PM) have significant impact on health and environment. The ability of aerosol to produce reactive oxygen species (ROS) is one of the widely proposed mechanisms related to the negative effects of aerosol on human health. Oxidative stress, caused by the production of ROS, is associated with several cardiovascular and respiratory diseases.\nThe capacity of aerosol to produce ROS is also called oxidative potential (OP). Although ROS generation capacity of PM exhibits a good correlation with PM mass concentration, it may depend more on physico-chemical properties of PM. Elements bound to PM play a significant role in contributing to the OP of aerosol. Exposure to elements was linked with several health issues (e.g. respiratory and cardiovascular diseases, asthma, inflammatory effects, lung cancer).
Keywords:
urban aerosol; bioaccessibility of elements; oxidative potential
Available in digital repository of the ASCR
Biodostupnost prvků a oxidativní potenciál městského aerosolu
Atmosférické aerosoly (ParticulateMatter, PM) mají významný dopad na zdraví a životní prostředí. Schopnost aerosolů produkovat reaktivní formy kyslíku (ROS) je jedním z často navrhovaných mechanismů ...
Separation of biological agents by divergent-flow isoelectric focusing as a tool for their rapid identification by MALDI-TOF MS
Duša, Filip; Šalplachta, Jiří; Horká, Marie; Lunerová, K.; Rosenbergová, K.; Kubíček, O.
2022 - English
We presented DF-IEF chip which proved as an efficient method for separation of biological agents, and it enabled sensitive identification of inactivated microorganisms with MALDI-TOF MS compared to unseparated samples.
Keywords:
bacteria; isoelectric; divergent
Available in digital repository of the ASCR
Separation of biological agents by divergent-flow isoelectric focusing as a tool for their rapid identification by MALDI-TOF MS
We presented DF-IEF chip which proved as an efficient method for separation of biological agents, and it enabled sensitive identification of inactivated microorganisms with MALDI-TOF MS compared to ...
Development of portable ultra-high performance liquid chromatograph
Šesták, Jozef; Gogaľová, Zuzana; Lunerová, K.
2022 - English
Recently we have successfully proved the concept of experimental portable liquid chromatograph which utilizes a simple hydraulic scheme and a universal optical detector capable of simultaneous absorbance and fluorescence monitoring. Here we present some latest improvements of our concept.
Keywords:
portable liquid chromatograph; miniaturization; microcolumn liquid chromatography
Available in digital repository of the ASCR
Development of portable ultra-high performance liquid chromatograph
Recently we have successfully proved the concept of experimental portable liquid chromatograph which utilizes a simple hydraulic scheme and a universal optical detector capable of simultaneous ...
Multi-cationic aminopyrene-based tag for oligosaccharide analysis by capillary electrophoresis with laser-induced fluorescence detection
Smolková, Denisa; Duša, Filip; Čmelík, Richard; Lavická, Jana
2022 - English
We present further optimization of the labeling method with a new multi-cationic aminopyrene-based tag, APTMP (4,4’,4’’-(8-aminopyrene-1,3,6-trisulfonyl)tris(1-methylpiperazine) in order to achieve higher resolution and sensitivity of CE/LIF analysis of APTMP labeled oligosaccharides.
Keywords:
oligosaccharides labeling; CE/LIF; APTMP
Available in digital repository of the ASCR
Multi-cationic aminopyrene-based tag for oligosaccharide analysis by capillary electrophoresis with laser-induced fluorescence detection
We present further optimization of the labeling method with a new multi-cationic aminopyrene-based tag, APTMP (4,4’,4’’-(8-aminopyrene-1,3,6-trisulfonyl)tris(1-methylpiperazine) in order to achieve ...
The map of quercetin and its derivatives occurence in parts of onion (Allium cepa L.) varieties
Benešová, K.; Lojková, L.; Kudláčková, Barbora
2022 - English
Determination of quercetin and its derivatives in dependency on the onion variety and the part from which it was extracted.
Keywords:
onion; quercetin; pressurized liquid extraction
Available in digital repository of the ASCR
The map of quercetin and its derivatives occurence in parts of onion (Allium cepa L.) varieties
Determination of quercetin and its derivatives in dependency on the onion variety and the part from which it was extracted.
Organické sloučeniny a markery ze spalování dřeva v kotlích používaných pro vytápění domácností
Křůmal, Kamil; Mikuška, Pavel; Horák, J.; Jaroch, M.; Hopan, F.; Kuboňová, L.
2022 - Czech
V emisích ze spalování tvrdého a měkkého dřeva byly měřeny organické sloučeniny (polycyklické aromatické uhlovodíky) a organické markery (monosacharidové anhydridy, diterpenoidy) používané pro identifikaci zdrojů emisí aerosolů. Dřevo bylo spalováno v různých kotlích používaných k vytápění bytů v České republice, tj. v protápěcím kotli, kotli s tahem spalování, zplyňovacím kotli a automatickém kotli. Emisní faktory částic a všech měřených organických sloučenin byly nejvyšší ze spalování paliv v nejstarším (přehořívacím) kotli. Organic compounds (polycyclic aromatic hydrocarbons) and organic markers (monosaccharide anhydrides, diterpenoids) used for the identification of aerosol emission sources were measured in the emissions from the combustion of hard and soft wood. Wood was combusted in different boilers used for the residential heating in the Czech Republic, i.e., overfire boiler, boiler with down-draft combustion, gasification boiler and automatic boiler. Emission factors of particles and all measured organic compounds were the highest from the combustion of fuels in the oldest (overfire) boiler.
Keywords:
residential heating; wood combustion; levoglucosan
Available in digital repository of the ASCR
Organické sloučeniny a markery ze spalování dřeva v kotlích používaných pro vytápění domácností
V emisích ze spalování tvrdého a měkkého dřeva byly měřeny organické sloučeniny (polycyklické aromatické uhlovodíky) a organické markery (monosacharidové anhydridy, diterpenoidy) používané pro ...
Bioluminescent probe for caspase imaging inside cells based on Foerster resonance energy transfer between quantum dot and quencher
Procházková, Markéta; Klepárník, Karel
2021 - English
In this work testing of novel quantum dot luminescent probe is presented. The quantum dot FRET-based luminescent probe enables long-time luminescent imaging of active caspases in living cells under microscope.
Keywords:
caspase; fluorescence; quantum dot
Available in digital repository of the ASCR
Bioluminescent probe for caspase imaging inside cells based on Foerster resonance energy transfer between quantum dot and quencher
In this work testing of novel quantum dot luminescent probe is presented. The quantum dot FRET-based luminescent probe enables long-time luminescent imaging of active caspases in living cells under ...
Novel photochemical vapor generation of rhenium for its ultratrace determination
Vyhnanovský, Jaromír; Hašlová, K.; Musil, Stanislav
2021 - English
The presentation focused on novel photochemical vapor generation of rhenium. Details on optimization of generation conditions and other important parameters regarding this sensitive methodology, as well as its possible applications were pointed out and discussed.
Keywords:
photochemical vapor generation; inductively coupled plasma mass spectrometry; rhenium
Available in digital repository of the ASCR
Novel photochemical vapor generation of rhenium for its ultratrace determination
The presentation focused on novel photochemical vapor generation of rhenium. Details on optimization of generation conditions and other important parameters regarding this sensitive methodology, as ...
Nanospray processing of silver nanoparticles for formation of dried deposits
Týčová, Anna; Jonas, Vladimír; Přikryl, Jan; Kotzianová, A.; Velebný, V.; Foret, František
2021 - English
Nanospray transfers liquid into an aerosol via electrostatic forces created between an emitter and a counter electrode. Herein, we present compact laboratory-made instrumentation for nanospray-processing of silver colloid. The instrumentation is based on the pressurized polysulfone chamber fixing a disposable vial with an immersed platinum electrode and a long fused silica capillary supplying the sprayed liquid to the emitter. The combination of low flow rates (70 nL/min) with a sharp emitter, fabricated on a 3D printed grinding station, resulted in a fine aerosol. The low volume of released droplets allowed full evaporation of water during their flight towards the counter electrode without any need for drying gas or the addition of volatile solvents. The constructed device was successfully used for the deposition of water-free silver colloid without any requirement of its pretreatment. The deposition of completely dried nanoparticles on planar substrates eliminated undesirable coffee ring effect and deposits of increased homogeneity could be obtained. Electron microscopy confirmed no significant changes in the character of nanospray-processed nanoparticles. Finally, we also investigated several approaches for the improvement of the surface density of nanoparticles on the substrate at preserved time scale.
Keywords:
nanoparticles; nanospray; silver
Available in digital repository of the ASCR
Nanospray processing of silver nanoparticles for formation of dried deposits
Nanospray transfers liquid into an aerosol via electrostatic forces created between an emitter and a counter electrode. Herein, we present compact laboratory-made instrumentation for ...
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