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Low-cost 3D-printed fluorescence detector for capillary electrophoresis
Přikryl, Jan; Foret, František
2013 - English
We have verified the possibility of Fused Deposition Modeling (type of additive manufacturing) for lab-made construction of inexpensive fluorescence detector for capillary electrophoresis using commercially available optical components. Sensitivity of fabricated and assembled detection system was tested by fluorescein solutions. Limits of detection were determined for several tested arrangements.
Keywords:
flourescence detector; capillary electrophoresis; 3D printing
Available on request at various institutes of the ASCR
Low-cost 3D-printed fluorescence detector for capillary electrophoresis
We have verified the possibility of Fused Deposition Modeling (type of additive manufacturing) for lab-made construction of inexpensive fluorescence detector for capillary electrophoresis using ...
Determination of chlorophenols in environmental samples using electromembrane extraction and capillary electrophoresis
Šlampová, Andrea; Kubáň, Pavel; Boček, Petr
2013 - English
Combination of electromembrane extraction (EME) with capillary electrophoresis (CE) was used for determination of trace level chlorophenols (CPs) in environmental water samples. The analytes were transported across supported liquid membrane (SLM), composed of 1-ethyl-2-nitrobenzene (ENB), by the application of electrical field. A driving force of 150 V was applied to extract the analytes from neutral sample (donor solution) into strongly alkaline acceptor solutions. The acceptor solutions were subsequently analyzed by CE with UV-Vis detector.
Keywords:
chlorophenols; electromembrane extraction; capillary electrophoresis
Available on request at various institutes of the ASCR
Determination of chlorophenols in environmental samples using electromembrane extraction and capillary electrophoresis
Combination of electromembrane extraction (EME) with capillary electrophoresis (CE) was used for determination of trace level chlorophenols (CPs) in environmental water samples. The analytes were ...
Application of pressurized hot water for etching of microfluidics
Vašina, M.; Grym, Jakub; Foret, František
2013 - English
The subject of this study is a new method of glass etching and its application in microfluidics. Commonly, hydrofluoric acid is used for glass etching. In this study we have tested a new method of glass etching using pressurized hot water. Test motives were prepared using photolitography and metal sputtering on the glass substrate and the etching was performed in a newly developed device capable to compress and heat water to its critical point. Electron microscopy and profilometry have been used for analyzing the surface of the etched glass substrate.
Keywords:
microfluidics; pressurized hot water
Available at various institutes of the ASCR
Application of pressurized hot water for etching of microfluidics
The subject of this study is a new method of glass etching and its application in microfluidics. Commonly, hydrofluoric acid is used for glass etching. In this study we have tested a new method of ...
Optimization of N-glycopeptides analysis methods and their preliminary application to barley proteins study
Benkovská, Dagmar; Flodrová, Dana; Bobálová, Janette; Laštovičková, Markéta
2013 - English
N-glycosylation is the most frequently studied plant protein post-translational modification. The analysis of Nglycopeptides after protein proteolytic digestion offers information about the structure of both oligosaccharide and peptide moiety. However, this method has so far been less commonly used. Since glycopeptides hardly ionize during MS analysis in the presence of non-glycosylated peptides, they need to be separated from the complex peptide mixture. In this study, the glycopeptides enrichment, purification and analysis methods were successfully optimized on two standard N-glycoproteins. Concanavalin A (ConA) lectin tips were used for glycopeptide capturing, and obtained fractions were purified on carbon tips and analyzed using MALDI-TOF mass spectrometry. The differences in the CID fragmentation of certain types of glycopeptides were found. This technique was then applied to glycopeptide analysis of barley grain and malt proteins. Several barley glycopeptides were found, however, their identification was very difficult. More proteins separation techniques will be required before this enrichment procedure in further studies.
Keywords:
glycosylation; post-translational modification; barley; N-glycopetptides
Available on request at various institutes of the ASCR
Optimization of N-glycopeptides analysis methods and their preliminary application to barley proteins study
N-glycosylation is the most frequently studied plant protein post-translational modification. The analysis of Nglycopeptides after protein proteolytic digestion offers information about the ...
Analysis of tissue-bound cells: Novel approaches using laser capture microdissection
Matalová, Eva; Adamová, Eva; Klepárník, Karel
2013 - English
Recent biomedical research prefers analysis of homogenous samples obtained from primary cells to cell lines. Moreover, attention is paid to cells of low number but high biological impact, such as signalling centres in embryonic development, stem cells or tumour cells. To localize cells of interest within an organ/tissue, histological sections are widely used. However, the methods of choice for further analysis of such samples are mostly limited to histochemistry, immunohistochemistry and in situ hybridization.
Keywords:
apoptosis; laser capture microdissection; cell analysis
Available on request at various institutes of the ASCR
Analysis of tissue-bound cells: Novel approaches using laser capture microdissection
Recent biomedical research prefers analysis of homogenous samples obtained from primary cells to cell lines. Moreover, attention is paid to cells of low number but high biological impact, such as ...
Proteomic evaluation of MCF-7 human breast cancer cells after treatment with retinoic acid isomers: Preliminary insights
Flodrová, Dana; Benkovská, Dagmar; Macejová, D.; Bialešová, L.; Brtko, J.; Bobálová, Janette
2012 - English
The effects of 9-cis retinoic acid and all-trans retinoic acid in human MCF-7 breast cancer line have been investigated. The total cell proteins were extracted and separated on 1D SDS-PAGE. The proteins were subsequently in-gel digested by trypsin and identified by MALDI-TOF/TOF.
Keywords:
retinoids; breast cancer; proteomic analysis
Available at various institutes of the ASCR
Proteomic evaluation of MCF-7 human breast cancer cells after treatment with retinoic acid isomers: Preliminary insights
The effects of 9-cis retinoic acid and all-trans retinoic acid in human MCF-7 breast cancer line have been investigated. The total cell proteins were extracted and separated on 1D SDS-PAGE. The ...
Unique exposure system for the whole body inhalation experiments with small animals
Večeřa, Zbyněk; Mikuška, Pavel; Dočekal, Bohumil; Vojta, Šimon; Míšek, Ivan; Moravec, Pavel; Smolík, Jiří
2011 - English
The especially inhalation chamber is constructed for chronic exposure studies of small animals to nanoparticles under strictly controlled conditions in frame of project „Study of transport of inhalated nano-sized particles (Pb, Cd) and their allocation in organs“. The inhalation chamber builds from a glass and stainless steel and makes possible long-term inhalation procedures (up to 60 days, 24 hours/day) with up to four discrete groups of mice population (up to 60 individuals each) or of rats population (up to 20 individuals each) under controlled illumination, temperature, relative humidity, doses (0.5 x 106 - 5 x 106 particles/cm3) and size (10-60 nm) of nano-sized particles which are synthesized via aerosol route in a hot wall tube flow reactor using metal organic precursors.
Keywords:
inhalation chamber; chronic exposure studies of small animals; long-term inhalation procedures
Available at various institutes of the ASCR
Unique exposure system for the whole body inhalation experiments with small animals
The especially inhalation chamber is constructed for chronic exposure studies of small animals to nanoparticles under strictly controlled conditions in frame of project „Study of transport of ...
Unique exposure system for the whole body inhalation experiments with small animals
Večeřa, Zbyněk; Mikuška, Pavel; Moravec, Pavel; Smolík, Jiří
2011 - English
The especially inhalation chamber is constructed for chronic exposure studies of small animals to nanoparticles under strictly controlled conditions in frame of project „Study of transport of inhalated nano-sized particles (Pb, Cd) and their allocation in organs“. The inhalation chamber builds from a glass and stainless steel and makes possible long-term inhalation procedures (up to 60 days, 24 hours/day) with up to four discrete groups of mice population (up to 60 individuals each) or of rats population (up to 20 individuals each) under controlled illumination, temperature, relative humidity, doses (0.5 x 106 - 5 x 106 particles/cm3) and size (10-60 nm) of nano-sized particles which are synthesized via aerosol route in a hot wall tube flow reactor using metal organic precursors.
Keywords:
inhalation chamber; chronic exposure studies of small animals; long-term inhalation procedures
Available at various institutes of the ASCR
Unique exposure system for the whole body inhalation experiments with small animals
The especially inhalation chamber is constructed for chronic exposure studies of small animals to nanoparticles under strictly controlled conditions in frame of project „Study of transport of ...
Seasonal variation in chemical composition of submicron urban aerosol in Brno
Mikuška, Pavel; Křůmal, Kamil; Večeřa, Zbyněk; Vojtěšek, Martin
2011 - English
Submicron aerosol particles in the size fraction PM1 collected in Brno in winter and summer of 2009 were analysed for both selected inorganic (ions, metals) and organic components (PAHs, hopanes and steranes, monosaccharide anhydrides, monocarboxylic acids). Special attention was paid to analysis of organic molecular markers to identify the major emission sources of aerosols.
Keywords:
submicron aerosol; chemical composition; emission sources
Available at various institutes of the ASCR
Seasonal variation in chemical composition of submicron urban aerosol in Brno
Submicron aerosol particles in the size fraction PM1 collected in Brno in winter and summer of 2009 were analysed for both selected inorganic (ions, metals) and organic components (PAHs, hopanes and ...
Immunofluorescent labeling of tissues and free cells via QD-protein conjugates
Lišková, Marcela; Voráčová, Ivona; Hezinová, Věra; Klepárník, Karel; Foret, František
2011 - English
Preparation of quantum dots and using their QD-protein conjugates in immunofluorescent labeling of cells and tissues. For conjugation was used method viaEDC/sulfo-NHS zero-length cross-linkers. We linked QD with annexin V, anti-CD3 and anti-PCNA. We labeled T-lymphocytes, apoptotic lymphocyte, mouse duodenum tissue and tumor tissue.
Keywords:
quantum dots; cell labeling; conjugation
Available at various institutes of the ASCR
Immunofluorescent labeling of tissues and free cells via QD-protein conjugates
Preparation of quantum dots and using their QD-protein conjugates in immunofluorescent labeling of cells and tissues. For conjugation was used method viaEDC/sulfo-NHS zero-length cross-linkers. We ...
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