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Hexahistidine-based tag for capillary electrophoresis-mass spektrometry analysis of oligosaccharides and N-linked glycans
Partyka, Jan; Křenková, Jana; Čmelík, Richard; Foret, František
2018 - anglický
Various basic amino acids and peptides were studied as an alternative labels for capillary electrophoresis-mass spectrometry analysis of oligosaccharides. The hexahistidine-based label with big number of positive charges allowed to use effective and sensitive CE-MS analysis in positive ion mode both neutral and acidic glycans.
Klíčová slova:
CE-MS analysis; oligosaccharides; amino acids; peptides
Dokument je dostupný na externích webových stránkách.
Hexahistidine-based tag for capillary electrophoresis-mass spektrometry analysis of oligosaccharides and N-linked glycans
Various basic amino acids and peptides were studied as an alternative labels for capillary electrophoresis-mass spectrometry analysis of oligosaccharides. The hexahistidine-based label with big number ...
Epitachophoretic separation and concentration of large volume samples
Voráčová, Ivona; Foret, František; Datinská, Vladimíra; Novotný, Jakub; Gheibi, P.; Berka, J.; Astier, Y.
2018 - anglický
There has been a growing interest in developing isotachophoretic protocols for concentration and purification of DNA as an alternative to the solid phase extraction protocols of DNA preparation. Also concentration of DNA from large volume samples is required. We have developed a new device suitable for separation, concentration and collection of ions from several milliliter sample volumes into microliter fractions. We have used a discontinuous electrolyte system comprising of the leading and terminating electrolytes utilized for concentration of samples containing DNA fragments. The method is simple, fast with high concentration factor and without extensive surface interactions.
Klíčová slova:
epitachophoretic separation; DNA; large sample volumes
Dokument je dostupný na externích webových stránkách.
Epitachophoretic separation and concentration of large volume samples
There has been a growing interest in developing isotachophoretic protocols for concentration and purification of DNA as an alternative to the solid phase extraction protocols of DNA preparation. Also ...
Hollow fibre-liquid phase microextraction of basic drugs from dried dried blood spots
Dvořák, Miloš; Miková, B.; Šlampová, Andrea; Kubáň, Pavel
2018 - anglický
Hollow fibre-liquid phase microextraction (HF-LPME) was applied for rapid and efficient extraction of basic drugs from dried blood spots (DBS). Basic drugs from DBS were first extracted into 10 mM NaOH solution, which acted as the HF-LPME donor solution, and simultaneously the neutralized drugs were transferred across the HF and preconcentrated in 10 mM HCl acceptor solution inside the HF lumen. Mixture (1:1, v:v) of 1-ethyl-2-nitrobenzene and dihexyl ether was used for impregnation of the porous HF membrane. Analytes in the acceptor solution were injected directly from the HF into capillary electrophoresis instrument for further separation, detection and quantification. Repeatability of the hyphenated analytical system ranged from 9.8 to 13.8 % (RSD) and enrichment factors of 52 – 78 were obtained for model basic drugs spiked to DBS extracts at a concentration level of 0.1 mg/L.
Klíčová slova:
hollow fibre-liquid phase microextraction; microextraction of basic drugs; dried blood spots
Dokument je dostupný na externích webových stránkách.
Hollow fibre-liquid phase microextraction of basic drugs from dried dried blood spots
Hollow fibre-liquid phase microextraction (HF-LPME) was applied for rapid and efficient extraction of basic drugs from dried blood spots (DBS). Basic drugs from DBS were first extracted into 10 mM ...
Surface enhanced raman spectroscopy: Detection platforms for sample analysis with and without electrophoretic separation
Týčová, Anna; Přikryl, Jan; Foret, František; Belder, D.
2018 - anglický
In this work we present two platforms of relatively simple construction for user-friendly sample detection via surface enhanced Raman spectroscopy (SERS). The first platform is designed for direct sample analysis and deals with hydrophilic spots array within a hydrophobic surface. The second presented platform aims on analysis of samples of higher complexity requiring separation step prior the detection. This is achived via microfluidic electrophoretic device with online SERS detection.
Klíčová slova:
raman spectroscopy; SERS; microfluidic electrophoretic device
Dokument je dostupný na externích webových stránkách.
Surface enhanced raman spectroscopy: Detection platforms for sample analysis with and without electrophoretic separation
In this work we present two platforms of relatively simple construction for user-friendly sample detection via surface enhanced Raman spectroscopy (SERS). The first platform is designed for direct ...
Speciation analysis of As and Hg in biological material
Petry-Podgórska, Inga; Matoušek, Tomáš; Migašová, M.; Zemanová, Veronika; Pavlík, Milan; Pavlíková, D.; Kratzer, Jan
2018 - anglický
This work was focused on an analysis of arsenic in selected plants and mercury in hair. A combination of HPLC and ICP-MS was used for analysis of biological tissues extracts. The\nspeciation analysis of low molecular arsenic and mercury species like inorganic iAs3+ and iAs5+, methylarsenic and dimethylarsenic forms was carried out with hydride generation (HG)\ntechnique, arsenic peptides and inorganic mercury (iHg2+) and its methylated form (MHg+) - with reverse phase (RP) chromatography and detected by ICP-MS.
Klíčová slova:
mercury; arsenic; speciation
Dokument je dostupný na externích webových stránkách.
Speciation analysis of As and Hg in biological material
This work was focused on an analysis of arsenic in selected plants and mercury in hair. A combination of HPLC and ICP-MS was used for analysis of biological tissues extracts. The\nspeciation analysis ...
Synthetic approaches for new labeling tags facilitating glycan analysis
Čmelík, Richard; Křenková, Jana; Foret, František
2018 - anglický
This study is focused on the synthesis of labels based on the pyrene core bearing several ionizable quaternary amines and the efficient carbonyl-reactive group, such as primary amine or hydrazine.
Klíčová slova:
labeling; organic synthesis; glycan
Dokument je dostupný na externích webových stránkách.
Synthetic approaches for new labeling tags facilitating glycan analysis
This study is focused on the synthesis of labels based on the pyrene core bearing several ionizable quaternary amines and the efficient carbonyl-reactive group, such as primary amine or hydrazine.
Evaluation of the 33 synthesized nitrophenol-based markers of isoelectic point
Duša, Filip; Moravcová, Dana; Šlais, Karel
2018 - anglický
33 nitrophenol-based compounds, which absorb at ultraviolet as well as visible wavelengths, were selected as candidates for marking isoelectric points (pI) over the pH range from 3 to 10. The evaluation and selection of the candidate structures included structure-based calculations of acid-base properties. The selected compounds were further tested experimentally using capillary isoelectric focusing. The experimental analysis excluded two compounds due to producing double peaks during the analysis. Overall, 31 novel compounds were identified as suitable pI markers.
Klíčová slova:
nitrophenol-based markers; isoelectic point; capillary isoelectric focusing
Dokument je dostupný na externích webových stránkách.
Evaluation of the 33 synthesized nitrophenol-based markers of isoelectic point
33 nitrophenol-based compounds, which absorb at ultraviolet as well as visible wavelengths, were selected as candidates for marking isoelectric points (pI) over the pH range from 3 to 10. The ...
Chromatographic separation of aflatoxin analogues
Moravcová, Dana; Planeta, Josef; Lunerová, K.; Duša, Filip; Šesták, Jozef; Kubíček, J.; Horká, Marie
2018 - anglický
Three short particle-packed columns (50 x 2.1 mm) were compared for the rapid and efficient chromatographic separation of aflatoxin G1, G2, B1 and B2 analogues.
Klíčová slova:
aflatoxin; HPLC; C18 stationary phase
Dokument je dostupný na externích webových stránkách.
Chromatographic separation of aflatoxin analogues
Three short particle-packed columns (50 x 2.1 mm) were compared for the rapid and efficient chromatographic separation of aflatoxin G1, G2, B1 and B2 analogues.
Arduino-based pressure-driven flow controller for droplet microfluidics
Přikryl, Jan; Křivánková, Jana; Hlaváček, Antonín
2018 - anglický
Droplet-based microfluidic systems bear significant value in an extremely wide range of applications due to its remarkable advantages, nevertheless, their technical demands on precise flow control are high. Here, we present lab-built Arduino-based flow controller fulfilling needs of droplet microfluidics to easily and effectively automate the system. As an additional required accessory, CNC-milled component for vials pressurizing was presented.
Klíčová slova:
droplet microfluidics; microfluidic systems; pressure regulator
Dokument je dostupný na externích webových stránkách.
Arduino-based pressure-driven flow controller for droplet microfluidics
Droplet-based microfluidic systems bear significant value in an extremely wide range of applications due to its remarkable advantages, nevertheless, their technical demands on precise flow control are ...
Rapid fabrication of microextraction devices for direct determination of basic drugs in dried blood spots
Ryšavá, Lenka; Přikryl, Jan; Dvořák, Miloš; Malá, Zdeňka; Kubáň, Pavel
2018 - anglický
A 3D-printed cutter was designed for rapid fabrication of supported liquid membrane (SLM) extraction devices compatible with injection system of commercial capillary electrophoresis (CE) instrument. Low cost and single use microextraction devices were fabricated from polypropylene micropipette tips with high precision (dimension variances ≤ 3% RSD). Practical applicability of the microextraction devices was examined by in-line coupling of SLM extractions to CE for direct analysis of four model basic drugs in dried blood spot extracts. Excellent repeatability of the hyphenated technique was achieved (RSD ≤ 7.6%), transfer of analytes across the SLM ranged from 23 to 73% and limits of detection were as low as 0.07 μg/mL.
Klíčová slova:
supported liquid membrane (SLM); capillary electrophoresis; microextraction devices
Dokument je dostupný na externích webových stránkách.
Rapid fabrication of microextraction devices for direct determination of basic drugs in dried blood spots
A 3D-printed cutter was designed for rapid fabrication of supported liquid membrane (SLM) extraction devices compatible with injection system of commercial capillary electrophoresis (CE) instrument. ...
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