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New sensor for DNA mutation detection
Datinská, Vladimíra; Klepárník, Karel; Minárik, M.; Foret, František
2013 - English
We present a design and synthesis of a new sensor intended for the genomic analysis in molecular cancer research. This sensor is based on the Förster resonance energy transfer (FRET) and can be used for the detection of complementary oligonucletide chains based on probe hybridization. Quantum dots (QDs) with their unique optical properties serve as suitable donors of energy in FRET.
Keywords:
quantum dots; Förster resonance energy transfer; CE-LIF
Available on request at various institutes of the ASCR
New sensor for DNA mutation detection
We present a design and synthesis of a new sensor intended for the genomic analysis in molecular cancer research. This sensor is based on the Förster resonance energy transfer (FRET) and can be ...
IEF and HPLC-based methods for efficient bacterial characterization
Kubesová, Anna; Šalplachta, Jiří; Moravcová, Dana; Horká, Marie
2013 - English
Pectobacterium and Dickeya species are responsible for blackleg and tuber soft rot diseases on crop and ornamental plants. Recently, a more virulent and aggressive Dickeya type of E. chrysanthemi was described and it becomes serious problem for potato production in Europe. In this study, we suggest procedures using two commonly available laboratory techniques, gel isoelectric focusing and liquid chromatography, for differentiation and characterization of Pectobacterium and Dickeya species. We have confirmed that fingerprinting approach can be used for bacterial differentiation, which can be further used in rapid diagnosis of plant disease.
Keywords:
Pectobacterium; Dickeya species; gel isoelectric focusing; liquid chromatography
Available on request at various institutes of the ASCR
IEF and HPLC-based methods for efficient bacterial characterization
Pectobacterium and Dickeya species are responsible for blackleg and tuber soft rot diseases on crop and ornamental plants. Recently, a more virulent and aggressive Dickeya type of E. chrysanthemi ...
Electrophoretic mobility measurements of a multivalent red dye SPADNS
Pantůčková, Pavla; Kubáň, Pavel; Boček, Petr
2013 - English
Measurements of the electrophoretic mobility of an anionic dye 4,5-dihydroxy-3-(p-sulfophenylazo)-2,7- naphthalene disulfonic acid trisodium salt (SPADNS), which is a commonly used anionic dye, are described in this contribution. Electrophoretic mobilities of SPADNS were measured in various buffer solutions covering the pH range of 2.4–9.1. Effective electrophoretic mobilities of SPADNS measured in these buffers varied between 50.5 x 10–9 m2/Vs and 60.5 x 10–9 m2/Vs with an average effective mobility of 55.0 x 10–9 m2/Vs.
Keywords:
electrophoretic mobility; SPADNS; electrophoretic separations
Available on request at various institutes of the ASCR
Electrophoretic mobility measurements of a multivalent red dye SPADNS
Measurements of the electrophoretic mobility of an anionic dye 4,5-dihydroxy-3-(p-sulfophenylazo)-2,7- naphthalene disulfonic acid trisodium salt (SPADNS), which is a commonly used anionic dye, are ...
Low-cost 3D-printed fluorescence detector for capillary electrophoresis
Přikryl, Jan; Foret, František
2013 - English
We have verified the possibility of Fused Deposition Modeling (type of additive manufacturing) for lab-made construction of inexpensive fluorescence detector for capillary electrophoresis using commercially available optical components. Sensitivity of fabricated and assembled detection system was tested by fluorescein solutions. Limits of detection were determined for several tested arrangements.
Keywords:
flourescence detector; capillary electrophoresis; 3D printing
Available on request at various institutes of the ASCR
Low-cost 3D-printed fluorescence detector for capillary electrophoresis
We have verified the possibility of Fused Deposition Modeling (type of additive manufacturing) for lab-made construction of inexpensive fluorescence detector for capillary electrophoresis using ...
Determination of chlorophenols in environmental samples using electromembrane extraction and capillary electrophoresis
Šlampová, Andrea; Kubáň, Pavel; Boček, Petr
2013 - English
Combination of electromembrane extraction (EME) with capillary electrophoresis (CE) was used for determination of trace level chlorophenols (CPs) in environmental water samples. The analytes were transported across supported liquid membrane (SLM), composed of 1-ethyl-2-nitrobenzene (ENB), by the application of electrical field. A driving force of 150 V was applied to extract the analytes from neutral sample (donor solution) into strongly alkaline acceptor solutions. The acceptor solutions were subsequently analyzed by CE with UV-Vis detector.
Keywords:
chlorophenols; electromembrane extraction; capillary electrophoresis
Available on request at various institutes of the ASCR
Determination of chlorophenols in environmental samples using electromembrane extraction and capillary electrophoresis
Combination of electromembrane extraction (EME) with capillary electrophoresis (CE) was used for determination of trace level chlorophenols (CPs) in environmental water samples. The analytes were ...
Application of pressurized hot water for etching of microfluidics
Vašina, M.; Grym, Jakub; Foret, František
2013 - English
The subject of this study is a new method of glass etching and its application in microfluidics. Commonly, hydrofluoric acid is used for glass etching. In this study we have tested a new method of glass etching using pressurized hot water. Test motives were prepared using photolitography and metal sputtering on the glass substrate and the etching was performed in a newly developed device capable to compress and heat water to its critical point. Electron microscopy and profilometry have been used for analyzing the surface of the etched glass substrate.
Keywords:
microfluidics; pressurized hot water
Available at various institutes of the ASCR
Application of pressurized hot water for etching of microfluidics
The subject of this study is a new method of glass etching and its application in microfluidics. Commonly, hydrofluoric acid is used for glass etching. In this study we have tested a new method of ...
Optimization of N-glycopeptides analysis methods and their preliminary application to barley proteins study
Benkovská, Dagmar; Flodrová, Dana; Bobálová, Janette; Laštovičková, Markéta
2013 - English
N-glycosylation is the most frequently studied plant protein post-translational modification. The analysis of Nglycopeptides after protein proteolytic digestion offers information about the structure of both oligosaccharide and peptide moiety. However, this method has so far been less commonly used. Since glycopeptides hardly ionize during MS analysis in the presence of non-glycosylated peptides, they need to be separated from the complex peptide mixture. In this study, the glycopeptides enrichment, purification and analysis methods were successfully optimized on two standard N-glycoproteins. Concanavalin A (ConA) lectin tips were used for glycopeptide capturing, and obtained fractions were purified on carbon tips and analyzed using MALDI-TOF mass spectrometry. The differences in the CID fragmentation of certain types of glycopeptides were found. This technique was then applied to glycopeptide analysis of barley grain and malt proteins. Several barley glycopeptides were found, however, their identification was very difficult. More proteins separation techniques will be required before this enrichment procedure in further studies.
Keywords:
glycosylation; post-translational modification; barley; N-glycopetptides
Available on request at various institutes of the ASCR
Optimization of N-glycopeptides analysis methods and their preliminary application to barley proteins study
N-glycosylation is the most frequently studied plant protein post-translational modification. The analysis of Nglycopeptides after protein proteolytic digestion offers information about the ...
Analysis of tissue-bound cells: Novel approaches using laser capture microdissection
Matalová, Eva; Adamová, Eva; Klepárník, Karel
2013 - English
Recent biomedical research prefers analysis of homogenous samples obtained from primary cells to cell lines. Moreover, attention is paid to cells of low number but high biological impact, such as signalling centres in embryonic development, stem cells or tumour cells. To localize cells of interest within an organ/tissue, histological sections are widely used. However, the methods of choice for further analysis of such samples are mostly limited to histochemistry, immunohistochemistry and in situ hybridization.
Keywords:
apoptosis; laser capture microdissection; cell analysis
Available on request at various institutes of the ASCR
Analysis of tissue-bound cells: Novel approaches using laser capture microdissection
Recent biomedical research prefers analysis of homogenous samples obtained from primary cells to cell lines. Moreover, attention is paid to cells of low number but high biological impact, such as ...
Proteomic evaluation of MCF-7 human breast cancer cells after treatment with retinoic acid isomers: Preliminary insights
Flodrová, Dana; Benkovská, Dagmar; Macejová, D.; Bialešová, L.; Brtko, J.; Bobálová, Janette
2012 - English
The effects of 9-cis retinoic acid and all-trans retinoic acid in human MCF-7 breast cancer line have been investigated. The total cell proteins were extracted and separated on 1D SDS-PAGE. The proteins were subsequently in-gel digested by trypsin and identified by MALDI-TOF/TOF.
Keywords:
retinoids; breast cancer; proteomic analysis
Available at various institutes of the ASCR
Proteomic evaluation of MCF-7 human breast cancer cells after treatment with retinoic acid isomers: Preliminary insights
The effects of 9-cis retinoic acid and all-trans retinoic acid in human MCF-7 breast cancer line have been investigated. The total cell proteins were extracted and separated on 1D SDS-PAGE. The ...
Depoziční tok ozonu nad smrkovým porostem a jeho korelace s čistou ekosystémovou produkcí
Zapletal, M.; Cudlín, Pavel; Chroust, P.; Urban, Otmar; Pokorný, Radek; Edwards-Jonášová, Magda; Czerný, Radek; Janouš, Dalibor; Taufarová, Klára; Medlen, A.; Večeřa, Zbyněk; Mikuška, Pavel; Skácel, F.
2011 - Czech
Srovnání výsledků měření a modelování depozičního a stomatálního toku ozonu u smrku ztepilého v lokalitě Bílý Kříž v červenci a srpnu 2008. Zvýšené koncentrace ozonu snižují čistou ekosystémovou produkci, zvláště při vyšších teplotách a vysoké intenzitě globálního záření. Measured and modelled results of ozone deposition and stomatal flux to a Norway spruce forest in locality Bílý Kříž in July and August 2008 were compared. Enhanced concentration of ozone decreased net ecosystem production, especially at higher temperatures and high intensity of global radiation.
Keywords:
ozone deposition flux; ozone stomatal flux; net ecosystem production
Available at various institutes of the ASCR
Depoziční tok ozonu nad smrkovým porostem a jeho korelace s čistou ekosystémovou produkcí
Srovnání výsledků měření a modelování depozičního a stomatálního toku ozonu u smrku ztepilého v lokalitě Bílý Kříž v červenci a srpnu 2008. Zvýšené koncentrace ozonu snižují čistou ekosystémovou ...
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