No (286)
Yes (207)
Number of found documents:
493
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A Validatation of non-invasive method for diagnostic of cystic fibrosis based on ion ratios determined by CE-C4D
Ďurč, P.; Lačná, J.; Dosedělová, V.; Foret, František; Homoľa, L.; Pokojová, E.; Malá, M.; Skřičková, J.; Dastych, M.; Vinohradská, H.; Dřevínek, P.; Skalická, V.; Kubáň, Petr
2018 - English
A validation of novel approach for diagnosis of cystic fibrosis (CF) is presented. The method is very simple and fast, since for collection of samples, only wiping of the skin of forearm is needed. The collected sample is analyzed by capillary electrophoresis with contactless conductometric detection (CE-C4D). Ion ratios are determined as ratios of peak area of chloride anion and peak areas of selected cations. It is possible to distinguish between cystic fibrosis patients and healthy individuals when ion ratio is applied as diagnostic factor. In here, we present analytical parameters of developed method (limits of detection, limits of quantification, repeatability of migration times, repeatability of peak areas) and ROC curve, which reveals high sensitivity and specificity of the developed method.
Keywords:
method for diagnostic of cystic fibrosis; cystic fibrosis; capillary electrophoresis with contactless conductometric detection
Fulltext is available at external website.
A Validatation of non-invasive method for diagnostic of cystic fibrosis based on ion ratios determined by CE-C4D
A validation of novel approach for diagnosis of cystic fibrosis (CF) is presented. The method is very simple and fast, since for collection of samples, only wiping of the skin of forearm is needed. ...
Acetonitrile-assisted enzymatic digestion used for analysis of proteins of cancer origin
Laštovičková, Markéta; Bobál, P.; Strouhalová, Dana; Bobálová, Janette
2018 - English
In this study, in-gel trypsin digestion protocol using aqueous-acetonitrile solvent system was developed to facilitate bottom-up proteomic identification of proteins isolated from human breast cancer cells.
Keywords:
breast cancer cells; enzymatic digestion; MS analysis
Fulltext is available at external website.
Acetonitrile-assisted enzymatic digestion used for analysis of proteins of cancer origin
In this study, in-gel trypsin digestion protocol using aqueous-acetonitrile solvent system was developed to facilitate bottom-up proteomic identification of proteins isolated from human breast cancer ...
Droplet-based microfluidic chip with passive mixer for analysis with photon-upconversion nanoparticles
Křivánková, Jana; Přikryl, Jan; Hlaváček, Antonín
2018 - English
Mixing of the fluids in microfluidic chips is important especially in applications, where droplet contents need to be rapidly homogenized. Here, a passive method for mixing in polydimethylsiloxane (PDMS) microchannels is presented. Droplet homogenization was firstly tested with organic stains and subsequently applied for mixing water dispersions of photon-upconversion nanoparticles (UCNPs).\n
Keywords:
microfluidic chips; microfluidic systems; photon-upconversion nanoparticles
Fulltext is available at external website.
Droplet-based microfluidic chip with passive mixer for analysis with photon-upconversion nanoparticles
Mixing of the fluids in microfluidic chips is important especially in applications, where droplet contents need to be rapidly homogenized. Here, a passive method for mixing in polydimethylsiloxane ...
Supercritical water in etching and morphing of siliceous surfaces for analytical separations
Karásek, Pavel; Horká, Marie; Planeta, Josef; Roth, Michal
2018 - English
Applications of supercritical water in etching and morphing of siliceous surfaces for analytical separations are briefly reviewed.
Keywords:
supercritical water; fused silica capillary; electromigration methods
Available in digital repository of the ASCR
Supercritical water in etching and morphing of siliceous surfaces for analytical separations
Applications of supercritical water in etching and morphing of siliceous surfaces for analytical separations are briefly reviewed.
Capillary electrophoresis in clinical diagnosis and monitoring
Kubáň, Petr
2018 - English
There is a significant potential of CE to provide a useful analytical tool to analyze noninvasive samples of body fluids and stimulate the cooperation between analytical chemists and clinicians in this interdisciplinary field.
Keywords:
CE; CE-LIF; clinical diagnosis
Available in digital repository of the ASCR
Capillary electrophoresis in clinical diagnosis and monitoring
There is a significant potential of CE to provide a useful analytical tool to analyze noninvasive samples of body fluids and stimulate the cooperation between analytical chemists and clinicians in ...
Measuring photon-upconversion luminiscence from droplets in microfluidic chips
Hlaváček, Antonín; Křivánková, Jana; Přikryl, Jan
2018 - English
UCNPs are lanthanide-doped nanocrystals that can be excited by near-infrared light and emit light of shorter wavelengths. Advantages of UCNPs include multiple and narrow emission bands, negligible autofluorescence and high photostability, which make UCNPs ideal luminescence label for use in droplet microfluidic. Here, we introduce the instrumentation for reading photon-upconversion luminescence of nanoparticles, which are dispersed in water droplets in a microfluidic chip.
Keywords:
photon-upconversion; microfluidic chip; microdroplets; nanoparticles
Fulltext is available at external website.
Measuring photon-upconversion luminiscence from droplets in microfluidic chips
UCNPs are lanthanide-doped nanocrystals that can be excited by near-infrared light and emit light of shorter wavelengths. Advantages of UCNPs include multiple and narrow emission bands, negligible ...
A Microfluidic devide for monitoring cell migration
Ledvina, Vojtěch; Klepárník, Karel; Legartová, Soňa; Bártová, Eva
2018 - English
A polydimethylsiloxane microfluidic device for monitoring directed cell migration was developed. The device comprises a main channel barred by pillar arrays with decreasing spacing and utilizes chemotactic movement of cells in the direction of increasing gradient of fetal bovine serum. HeLa cell line transfected with Premo FUCCI Cell Cycle Sensor was used as a model organism. The cells were imaged for several days using a scanning confocal microscope and the rate of migration in different cell cycle phases was evaluated.
Keywords:
microfluidic device; cell migration; Premo FUCCI Cell Cycle Sensor
Fulltext is available at external website.
A Microfluidic devide for monitoring cell migration
A polydimethylsiloxane microfluidic device for monitoring directed cell migration was developed. The device comprises a main channel barred by pillar arrays with decreasing spacing and utilizes ...
Affinity microfluidic chip based on packed microbeads
Novotný, Jakub; Ostatná, Veronika; Foret, František
2018 - English
In this project, a plastic device was fabricated using CNC milling in polycarbonate. Chip contains integrated pneumatic valves for flow control and allows for injection and stacking of beaded supports. Intended use of these supports is formation of packed affinity columns suitable for glycoprotein assays.
Keywords:
microfluidic chips; microfluidic systems; microfluidic devices
Fulltext is available at external website.
Affinity microfluidic chip based on packed microbeads
In this project, a plastic device was fabricated using CNC milling in polycarbonate. Chip contains integrated pneumatic valves for flow control and allows for injection and stacking of beaded ...
Hexahistidine-based tag for capillary electrophoresis-mass spektrometry analysis of oligosaccharides and N-linked glycans
Partyka, Jan; Křenková, Jana; Čmelík, Richard; Foret, František
2018 - English
Various basic amino acids and peptides were studied as an alternative labels for capillary electrophoresis-mass spectrometry analysis of oligosaccharides. The hexahistidine-based label with big number of positive charges allowed to use effective and sensitive CE-MS analysis in positive ion mode both neutral and acidic glycans.
Keywords:
CE-MS analysis; oligosaccharides; amino acids; peptides
Fulltext is available at external website.
Hexahistidine-based tag for capillary electrophoresis-mass spektrometry analysis of oligosaccharides and N-linked glycans
Various basic amino acids and peptides were studied as an alternative labels for capillary electrophoresis-mass spectrometry analysis of oligosaccharides. The hexahistidine-based label with big number ...
Epitachophoretic separation and concentration of large volume samples
Voráčová, Ivona; Foret, František; Datinská, Vladimíra; Novotný, Jakub; Gheibi, P.; Berka, J.; Astier, Y.
2018 - English
There has been a growing interest in developing isotachophoretic protocols for concentration and purification of DNA as an alternative to the solid phase extraction protocols of DNA preparation. Also concentration of DNA from large volume samples is required. We have developed a new device suitable for separation, concentration and collection of ions from several milliliter sample volumes into microliter fractions. We have used a discontinuous electrolyte system comprising of the leading and terminating electrolytes utilized for concentration of samples containing DNA fragments. The method is simple, fast with high concentration factor and without extensive surface interactions.
Keywords:
epitachophoretic separation; DNA; large sample volumes
Fulltext is available at external website.
Epitachophoretic separation and concentration of large volume samples
There has been a growing interest in developing isotachophoretic protocols for concentration and purification of DNA as an alternative to the solid phase extraction protocols of DNA preparation. Also ...
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