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Subsidiary reagens for improvement of enzymatic digestion protocol
Laštovičková, Markéta; Bobáľ, P.; Flodrová, Dana; Bobálová, Janette
2016 - English
In this report, we demonstrate the influence of hydrogen peroxide, phenol and\nacetonitrile on enzymatic digestion of proteins accomplished during common bottomup\nproteomic identifications.
Keywords:
proteomic identifications; MALDI-TOF MS; enzymatic digestion; peptides
Available in digital repository of the ASCR
Subsidiary reagens for improvement of enzymatic digestion protocol
In this report, we demonstrate the influence of hydrogen peroxide, phenol and\nacetonitrile on enzymatic digestion of proteins accomplished during common bottomup\nproteomic identifications.
Determination of dicarboxylic acids in atmospheric aerosols using ion chromatography with on-line connected preconcentration unit
Čapka, Lukáš; Mikuška, Pavel; Večeřa, Zbyněk
2016 - English
A method for the fast and sensitive detection of dicarboxylic acids in atmospheric\naerosols was optimized. The method is based on the online sampling and subsequent\nanalysis with ion chromatography. The system was applied to the determination of\nDCAs in urban aerosols in Brno. The method was verified using a laboratory generated\nstandard aerosol of DCAs. A sampling of aerosol particles on parallel filter was used\nas a reference method.
Keywords:
atmospheric aerosols; dicarboxylic acids; aerosol collector; ion chromatography
Available in digital repository of the ASCR
Determination of dicarboxylic acids in atmospheric aerosols using ion chromatography with on-line connected preconcentration unit
A method for the fast and sensitive detection of dicarboxylic acids in atmospheric\naerosols was optimized. The method is based on the online sampling and subsequent\nanalysis with ion chromatography. ...
Investigation of bioactive proteins in pressurized water extract of Sambucus nigra L. branches
Hohnová, Barbora; Šalplachta, Jiří; Roth, Michal
2016 - English
Investigation of high-value substances in plant materials has been of increasing interest\nin last years. In this study, PHWE, SDS-PAGE and MALDI-TOF techniques were\nemployed for investigation of bioactive proteins in Sambucus nigra L. branches.
Keywords:
extraction of bioactive proteins; MALDI-TOF/TOF mass spectrometry; Sambucus nigra L. branches; SDS-PAGE; PHWE
Available in digital repository of the ASCR
Investigation of bioactive proteins in pressurized water extract of Sambucus nigra L. branches
Investigation of high-value substances in plant materials has been of increasing interest\nin last years. In this study, PHWE, SDS-PAGE and MALDI-TOF techniques were\nemployed for investigation of ...
Combined effect of temperature and CdO nanoparticles treatment on Picea abies
Večeřová, Kristýna; Mikuška, Pavel; Oravec, Michal; Kozáčiková, Michaela; Pompeiano, Antonio; Coufalík, Pavel; Urban, Otmar
2016 - English
Three-years old seedlings of Picea abies were pre-treated under two different\ntemperatures (20x35 °C) and subsequently exposed to elevated concentration of CdO\nnanoparticles (CdONPs). Two-week exposure to airborne CdONPs of ecologically\nrelevant size and concentration significantly reduced photosynthetic light use efficiency\nin plants as well as contents of photosynthetic pigments and saccharides. In contrary,\namino acid content was increased under CdONPs. These changes were further\nmodulated by previous growth temperature.
Keywords:
Picea abies; terrestrial ecosystems; CdO nanoparticles
Available in digital repository of the ASCR
Combined effect of temperature and CdO nanoparticles treatment on Picea abies
Three-years old seedlings of Picea abies were pre-treated under two different\ntemperatures (20x35 °C) and subsequently exposed to elevated concentration of CdO\nnanoparticles (CdONPs). Two-week ...
Highly sensitive analysis of chlorophenols and sulfonamides in waters by electrophoretic focusion on inverse electromigration dispersion gradient with ESI-MS detection
Malá, Zdeňka; Gebauer, Petr; Boček, Petr
2016 - English
This work presents a new methodology for high-sensitivity analyses by capillary\nelectrophoresis (CE) with electrospray-ionization mass spectrometric (ESI-MS)\ndetection, based on electrophoretic focusing on an inverse electromigration dispersion\n(EMD) gradient. The technique is based on a robust electrolyte system providing a\ngradient of required properties, suitable for routine analyses of trace amounts of weak\nacids with pKa values between approx. 6.5 and 9. Examples analyses of several\nchlorophenols and sulfonamides are presented, with LODs around 3x10-9 M for spiked\ndrinking water without any sample pretreatment.
Keywords:
capillary electrophoresis; electrospray-ionization mass spectrometric detection; chlorophenols; sulfonamides
Available in digital repository of the ASCR
Highly sensitive analysis of chlorophenols and sulfonamides in waters by electrophoretic focusion on inverse electromigration dispersion gradient with ESI-MS detection
This work presents a new methodology for high-sensitivity analyses by capillary\nelectrophoresis (CE) with electrospray-ionization mass spectrometric (ESI-MS)\ndetection, based on electrophoretic ...
Charakterizace organických sloučenin v PM1 aerosolu v Kladně v zimním období 2016
Křůmal, Kamil; Mikuška, Pavel; Večeřa, Zbyněk
2016 - Czech
Organické sloučeniny a markery používané pro identifikaci hlavních emisních zdrojů byly měřeny v PM1 aerosolu v malém městě Kladně-Švermově. Nejvyšší koncentrace byly pro anhydridy monosacharidů a pryskyřičné kyseliny (spalování biomasy). Dalšími analyzovanými skupinami organických sloučenin a markerů byly polycyklické aromatické uhlovodíky (emise z dopravy a nedokonalého spalování), hopany (spalování uhlí, doprava), sterany (doprava), sacharidy a alkany. In this work the organic compounds and organic markers used for the identification of main aerosol sources were measured in PM1 aerosol in a small town (Kladno-Švermov) near Prague. Monosaccharide anhydrides and resin acids (emissions from biomass combustion) were found especially. Polycyclic aromatic hydrocarbons (emissions from traffic and incomplete combustion), hopanes (traffic, coal combustion), steranes (traffic), saccharides and alkanes were other groups of analysed organic compounds.
Keywords:
organic tracer; urban aerosol; PM1
Available in digital repository of the ASCR
Charakterizace organických sloučenin v PM1 aerosolu v Kladně v zimním období 2016
Organické sloučeniny a markery používané pro identifikaci hlavních emisních zdrojů byly měřeny v PM1 aerosolu v malém městě Kladně-Švermově. Nejvyšší koncentrace byly pro anhydridy monosacharidů a ...
A novel nanospray liquid junction interface for versatile CE-MS
Křenková, Jana; Haselberg, R.; Somsen, G. W.; Foret, František
2016 - English
In this work we present a new polyimide-based liquid junction nanospray interface, combining the advantages of sheath flow and sheathless designs. The liquid junction interface was prepared as a hybrid microfabricated plastic liquid junction sprayer allowing to perform analysis in standard (30-100 micro m ID) separation capillaries. The interface incorporates a self-aligning port for coupling of the separation capillary as well as ports for automated flushing of both the liquid junction and the separation capillary. The interface permits performing CE both with and without electroosmotic flow. The use of large bore capillaries allows injection and preconcentration of larger sample volumes, favoring detection sensitivity. Different compositions of the spray liquid and BGE can be used when needed.
Keywords:
capillary electrophoresis; mass spectrometry; liquid junction interface
Available in digital repository of the ASCR
A novel nanospray liquid junction interface for versatile CE-MS
In this work we present a new polyimide-based liquid junction nanospray interface, combining the advantages of sheath flow and sheathless designs. The liquid junction interface was prepared as a ...
Determination of levoglucosan in urban aerosol
Coufalík, Pavel; Mikuška, Pavel; Čmelík, Richard
2016 - English
The main aim of this work was a development of the analytical method for levoglucosan\ndetermination including direct collection of aerosol into water phase, preconcentration,\nand determination by LC-MS.
Keywords:
monosaccharide anhydrides; urban aerosol; levoglucosan
Available in digital repository of the ASCR
Determination of levoglucosan in urban aerosol
The main aim of this work was a development of the analytical method for levoglucosan\ndetermination including direct collection of aerosol into water phase, preconcentration,\nand determination by ...
Zvýšení účinnosti kontinuálního aerosolového vzorkovače
Mikuška, Pavel; Čapka, Lukáš; Večeřa, Zbyněk
2016 - Czech
Příspěvek se zabývá zvýšením účinnosti záchytu ACTJU vzorkovače i pro částice menší než 300 nm. Použitím tzv. „růstové trubky“ (RT), která je umístěna před vzorkovačem, vzroste velikost částic na několik µm a zvětšené částice jsou pak snadno kvantitativně zachyceny v ACTJU vzorkovači. Contribution deals with increasing of collection efficiency of ACTJU sampler also for particles smaller than 300 nm in diameter. Use of so-called „growth tube“ (GT) located upstream of the ACTJU results in increase of particle size to several µm. Enlarged particles are then easily quantitative collected in ACTJU sampler.
Keywords:
continuous sampler; on-line analysis; atmospheric aerosol
Available in digital repository of the ASCR
Zvýšení účinnosti kontinuálního aerosolového vzorkovače
Příspěvek se zabývá zvýšením účinnosti záchytu ACTJU vzorkovače i pro částice menší než 300 nm. Použitím tzv. „růstové trubky“ (RT), která je umístěna před vzorkovačem, vzroste velikost částic na ...
Effect of two retinoic acid isomers on the expression of selected nuclear retinoid receptors and protein pattern in human MCF-7 breast cancer cell line
Brtko, J.; Toporová, L.; Macejová, D.; Bialešová, L.; Flodrová, Dana; Bobálová, Janette
2016 - English
Effect of two retinoic acid isomers on RARalpha, RARgamma, RXRalpha, RXRbeta expression and protein profile in human MCF-7 breast cancer line were investigated.
Keywords:
retinoic acid; retinoid receptors; protein pattern
Available in digital repository of the ASCR
Effect of two retinoic acid isomers on the expression of selected nuclear retinoid receptors and protein pattern in human MCF-7 breast cancer cell line
Effect of two retinoic acid isomers on RARalpha, RARgamma, RXRalpha, RXRbeta expression and protein profile in human MCF-7 breast cancer line were investigated.
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