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Analysis of low abundant lipids in vernix caseosa using chromatographic methods and mass spectrometry
Háková, Eva; Míková, R.; Vrkoslav, Vladimír; Plavka, R.; Cvačka, Josef
2015 - anglický
The aim of this work is to develop a separation method for characterization of unknown lipids in vernix caseosa. We focused on very low abundant lipids which have not been previously described in vernix caseosa. The total lipid extract was fractionated using low pressure normal phase liquid chromatography to obtain sub-fractions. The sub-fractions were further separated in high performance liquid chromatography mode to isolate classes of lipids. This procedure enabled us to characterise lipid classes by mass spectrometry and identify one of the class as acyl-ceramides with non-hydroxylated fatty acids.
Klíčová slova:
lipids; high performance liquid chromatography; mass spectrometry; vernix caseosa
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Analysis of low abundant lipids in vernix caseosa using chromatographic methods and mass spectrometry
The aim of this work is to develop a separation method for characterization of unknown lipids in vernix caseosa. We focused on very low abundant lipids which have not been previously described in ...
Ionizační techniky
Vrkoslav, Vladimír
2015 - český
Byly popsány principy nejdůležitějších ionizačních technik, jejich rozdělení a příklady využití. Classification, principles and application examples of the most important ionization techniques were described.
Klíčová slova:
mass spectrometry; ion source; ionization
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Ionizační techniky
Byly popsány principy nejdůležitějších ionizačních technik, jejich rozdělení a příklady využití....
Mobilitní spektrometrie DMF/FAIMS
Cvačka, Josef
2015 - český
Byly popsány principy mobilitní spektrometrie DMF/FAIMS a byly uvedeny příklady využití. Principles and application examples of ion mobility spectrometry DMS/FAIMS were described.
Klíčová slova:
mass spectrometry; ion mobility; ion analyzers
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Mobilitní spektrometrie DMF/FAIMS
Byly popsány principy mobilitní spektrometrie DMF/FAIMS a byly uvedeny příklady využití.
Principles and application examples of ion mobility spectrometry DMS/FAIMS were described.
Mobilní hmotnostní spektrometrie
Cvačka, Josef
2015 - český
Byla vysvětlena specifika a příklady použití mobilních hmotnostních spektrometrů. Using and specifics of mobile mass spectrometers were explained.
Klíčová slova:
mass spectrometer; miniaturization; hand-held instruments
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Mobilní hmotnostní spektrometrie
Byla vysvětlena specifika a příklady použití mobilních hmotnostních spektrometrů.
Using and specifics of mobile mass spectrometers were explained.
Insect repellent and feeding deterrent activity of natural sesquiterpene lactones and their derivatives
Adekenov, S. M.; Mukhametzhanova, G. M.; Atazhanova, G. A.; Harmatha, Juraj
2015 - anglický
Thirty compounds were investigated in order to detect their repellent and antifeedant activity. The substances were tested on several insect test-objects: Sitophilus granarius, Tribolium confusum and Trogoderma granarium. Five sesquiterpene alpha-exometylene-gamma-lactones and one structurally transformed dimethylamino derivative showed high antifeedant and repellent activities.
Klíčová slova:
sesquiterpene lactones; stored product pests
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Insect repellent and feeding deterrent activity of natural sesquiterpene lactones and their derivatives
Thirty compounds were investigated in order to detect their repellent and antifeedant activity. The substances were tested on several insect test-objects: Sitophilus granarius, Tribolium confusum and ...
Chiroptical spectroscopy as a sensitive tool for the conformational analysis of antimicrobial peptides
Kocourková, L.; Novotná, P.; Čujová, Sabína; Čeřovský, Václav; Urbanová, M.; Setnička, V.
2015 - anglický
We tested the utilization of electronic and vibrational circular dichroism for the conformational study of antimicrobial peptides on two examples: melectin and antapin in different solutions of liposomes and micelles mimicking bacterial or eukaryotic membranes. The results show significant content of the alpha-helical conformation in an environment mimicking the bacterial membrane, while it was considerably low in the eukaryotic membrane-mimicking environment.
Klíčová slova:
antimicrobial peptides; circular dichroism; conformation; liposomes; model membranes
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Chiroptical spectroscopy as a sensitive tool for the conformational analysis of antimicrobial peptides
We tested the utilization of electronic and vibrational circular dichroism for the conformational study of antimicrobial peptides on two examples: melectin and antapin in different solutions of ...
Capillary electrophoretic study of solvent effects on complexation of dibenzo-18-crown-6 with potassium ions
Konášová, Renáta; Jaklová Dytrtová, Jana; Kašička, Václav
2015 - anglický
Affinity capillary electrophoresis was applied to investigation of solvent effects on stability constant of dibenzo-18-crown-6 complex with potassium ion in mixed hydro-organic solvents at different water/methanol, water/ethanol and water/propan-2-ol ratios. The apparent stability constants of the complex were found to be strongly decreasing with increasing molar fraction of water in the mixed solvents.
Klíčová slova:
affinity capillary electrophoresis; solvent effect; binding constant
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Capillary electrophoretic study of solvent effects on complexation of dibenzo-18-crown-6 with potassium ions
Affinity capillary electrophoresis was applied to investigation of solvent effects on stability constant of dibenzo-18-crown-6 complex with potassium ion in mixed hydro-organic solvents at different ...
Vernix caseosa and its newly discovered nonpolar lipids
Háková, Eva; Míková, Radka; Vrkoslav, Vladimír; Doležal, A.; Plavka, R.; Cvačka, Josef
2014 - anglický
The aim of this work is a description of newly, so far undescribed nonpolar lipids, which are components of vernix caseosa.
Klíčová slova:
2D-offline chromatography; mass spectrometry; lipids; vernix caseosa
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Vernix caseosa and its newly discovered nonpolar lipids
The aim of this work is a description of newly, so far undescribed nonpolar lipids, which are components of vernix caseosa.
Chiral analysis of alfa-diimine Ru(II) and Fe(II) complexes by capillary electrophoresis using sulfated cyclodextrins as stereoselectors
Sázelová, Petra; Koval, Dušan; Severa, Lukáš; Teplý, Filip; Kašička, Václav
2014 - anglický
A fast and efficient capillary electrophoretic method was developed for enantioseparation of Ru(II) and Fe(II) polypyridyl complexes using anionic randomly sulfated alfa-, beta-, and gamma-cyclodextrins (S-alfa-CD, S-beta-CD, and S-gamma-CD) and their heptakis-(2,3-diacetyl-6-sulfato)-derivatives (Ac-S-alfa-CD, Ac-S-beta-CD, and Ac-S-gamma-CD) as chiral selectors. S-alfa-CD and S-gamma-CD were found to be the best chiral selectors providing high resolutions and short analysis times. The method was applied for the assessment of chiral purity of some batches of [Ru(bpy)3]2+ catalyst.
Klíčová slova:
capillary electrophoresis; chiral analysis; metal-polypyridyl complexes
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Chiral analysis of alfa-diimine Ru(II) and Fe(II) complexes by capillary electrophoresis using sulfated cyclodextrins as stereoselectors
A fast and efficient capillary electrophoretic method was developed for enantioseparation of Ru(II) and Fe(II) polypyridyl complexes using anionic randomly sulfated alfa-, beta-, and ...
Polymerase synthesis of base-modified DNA: New methods and new applications
Balintová, Jana; Daďová, Jitka; Kielkowski, Pavel; Ménová, Petra; Vaníková, Zuzana; Riedl, Jan; Raindlová, Veronika; Fojta, Miroslav; Hocek, Michal
2014 - anglický
Diverse base-modified oligonucleotides and double-stranded DNA molecules were prepared by polymerase incorporation of modified nucleoside triphosphates. The methods include primer extension, PCR, nicking enzyme amplification reaction and mixed incorporations. The modified nucleic acids were used in redox and fluorescence labelling and coding, as well as regulation of binding and cross-linking with proteins.
Klíčová slova:
nucleic acids; base-modified DNA; dNTPs
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Polymerase synthesis of base-modified DNA: New methods and new applications
Diverse base-modified oligonucleotides and double-stranded DNA molecules were prepared by polymerase incorporation of modified nucleoside triphosphates. The methods include primer extension, PCR, ...
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