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Measuring photon-upconversion luminiscence from droplets in microfluidic chips
Hlaváček, Antonín; Křivánková, Jana; Přikryl, Jan
2018 - anglický
UCNPs are lanthanide-doped nanocrystals that can be excited by near-infrared light and emit light of shorter wavelengths. Advantages of UCNPs include multiple and narrow emission bands, negligible autofluorescence and high photostability, which make UCNPs ideal luminescence label for use in droplet microfluidic. Here, we introduce the instrumentation for reading photon-upconversion luminescence of nanoparticles, which are dispersed in water droplets in a microfluidic chip.
Klíčová slova:
photon-upconversion; microfluidic chip; microdroplets; nanoparticles
Dokument je dostupný na externích webových stránkách.
Measuring photon-upconversion luminiscence from droplets in microfluidic chips
UCNPs are lanthanide-doped nanocrystals that can be excited by near-infrared light and emit light of shorter wavelengths. Advantages of UCNPs include multiple and narrow emission bands, negligible ...
A Microfluidic devide for monitoring cell migration
Ledvina, Vojtěch; Klepárník, Karel; Legartová, Soňa; Bártová, Eva
2018 - anglický
A polydimethylsiloxane microfluidic device for monitoring directed cell migration was developed. The device comprises a main channel barred by pillar arrays with decreasing spacing and utilizes chemotactic movement of cells in the direction of increasing gradient of fetal bovine serum. HeLa cell line transfected with Premo FUCCI Cell Cycle Sensor was used as a model organism. The cells were imaged for several days using a scanning confocal microscope and the rate of migration in different cell cycle phases was evaluated.
Klíčová slova:
microfluidic device; cell migration; Premo FUCCI Cell Cycle Sensor
Dokument je dostupný na externích webových stránkách.
A Microfluidic devide for monitoring cell migration
A polydimethylsiloxane microfluidic device for monitoring directed cell migration was developed. The device comprises a main channel barred by pillar arrays with decreasing spacing and utilizes ...
Affinity microfluidic chip based on packed microbeads
Novotný, Jakub; Ostatná, Veronika; Foret, František
2018 - anglický
In this project, a plastic device was fabricated using CNC milling in polycarbonate. Chip contains integrated pneumatic valves for flow control and allows for injection and stacking of beaded supports. Intended use of these supports is formation of packed affinity columns suitable for glycoprotein assays.
Klíčová slova:
microfluidic chips; microfluidic systems; microfluidic devices
Dokument je dostupný na externích webových stránkách.
Affinity microfluidic chip based on packed microbeads
In this project, a plastic device was fabricated using CNC milling in polycarbonate. Chip contains integrated pneumatic valves for flow control and allows for injection and stacking of beaded ...
Hexahistidine-based tag for capillary electrophoresis-mass spektrometry analysis of oligosaccharides and N-linked glycans
Partyka, Jan; Křenková, Jana; Čmelík, Richard; Foret, František
2018 - anglický
Various basic amino acids and peptides were studied as an alternative labels for capillary electrophoresis-mass spectrometry analysis of oligosaccharides. The hexahistidine-based label with big number of positive charges allowed to use effective and sensitive CE-MS analysis in positive ion mode both neutral and acidic glycans.
Klíčová slova:
CE-MS analysis; oligosaccharides; amino acids; peptides
Dokument je dostupný na externích webových stránkách.
Hexahistidine-based tag for capillary electrophoresis-mass spektrometry analysis of oligosaccharides and N-linked glycans
Various basic amino acids and peptides were studied as an alternative labels for capillary electrophoresis-mass spectrometry analysis of oligosaccharides. The hexahistidine-based label with big number ...
Epitachophoretic separation and concentration of large volume samples
Voráčová, Ivona; Foret, František; Datinská, Vladimíra; Novotný, Jakub; Gheibi, P.; Berka, J.; Astier, Y.
2018 - anglický
There has been a growing interest in developing isotachophoretic protocols for concentration and purification of DNA as an alternative to the solid phase extraction protocols of DNA preparation. Also concentration of DNA from large volume samples is required. We have developed a new device suitable for separation, concentration and collection of ions from several milliliter sample volumes into microliter fractions. We have used a discontinuous electrolyte system comprising of the leading and terminating electrolytes utilized for concentration of samples containing DNA fragments. The method is simple, fast with high concentration factor and without extensive surface interactions.
Klíčová slova:
epitachophoretic separation; DNA; large sample volumes
Dokument je dostupný na externích webových stránkách.
Epitachophoretic separation and concentration of large volume samples
There has been a growing interest in developing isotachophoretic protocols for concentration and purification of DNA as an alternative to the solid phase extraction protocols of DNA preparation. Also ...
CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis
Foret, František; Křenková, Jana; Drobníková, Iveta; Klepárník, Karel; Přikryl, Jan
2018 - anglický
This year the meeting included full texts of 25 oral and 61 poster presentations. The attached conference proceedings, edited by highly cited scientists, documents the quality of the meeting. Similarly to the previous years the lectures were given by outstanding, highly cited, scientists. The list of speakers as well as more detailed information about the conference can be found on its permanent web at www.ce-ce.org.
Klíčová slova:
bioanalysis; separation; biotechnology
Dokument je dostupný na externích webových stránkách.
CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis
This year the meeting included full texts of 25 oral and 61 poster presentations. The attached conference proceedings, edited by highly cited scientists, documents the quality of the meeting. ...
Hollow fibre-liquid phase microextraction of basic drugs from dried dried blood spots
Dvořák, Miloš; Miková, B.; Šlampová, Andrea; Kubáň, Pavel
2018 - anglický
Hollow fibre-liquid phase microextraction (HF-LPME) was applied for rapid and efficient extraction of basic drugs from dried blood spots (DBS). Basic drugs from DBS were first extracted into 10 mM NaOH solution, which acted as the HF-LPME donor solution, and simultaneously the neutralized drugs were transferred across the HF and preconcentrated in 10 mM HCl acceptor solution inside the HF lumen. Mixture (1:1, v:v) of 1-ethyl-2-nitrobenzene and dihexyl ether was used for impregnation of the porous HF membrane. Analytes in the acceptor solution were injected directly from the HF into capillary electrophoresis instrument for further separation, detection and quantification. Repeatability of the hyphenated analytical system ranged from 9.8 to 13.8 % (RSD) and enrichment factors of 52 – 78 were obtained for model basic drugs spiked to DBS extracts at a concentration level of 0.1 mg/L.
Klíčová slova:
hollow fibre-liquid phase microextraction; microextraction of basic drugs; dried blood spots
Dokument je dostupný na externích webových stránkách.
Hollow fibre-liquid phase microextraction of basic drugs from dried dried blood spots
Hollow fibre-liquid phase microextraction (HF-LPME) was applied for rapid and efficient extraction of basic drugs from dried blood spots (DBS). Basic drugs from DBS were first extracted into 10 mM ...
Surface enhanced raman spectroscopy: Detection platforms for sample analysis with and without electrophoretic separation
Týčová, Anna; Přikryl, Jan; Foret, František; Belder, D.
2018 - anglický
In this work we present two platforms of relatively simple construction for user-friendly sample detection via surface enhanced Raman spectroscopy (SERS). The first platform is designed for direct sample analysis and deals with hydrophilic spots array within a hydrophobic surface. The second presented platform aims on analysis of samples of higher complexity requiring separation step prior the detection. This is achived via microfluidic electrophoretic device with online SERS detection.
Klíčová slova:
raman spectroscopy; SERS; microfluidic electrophoretic device
Dokument je dostupný na externích webových stránkách.
Surface enhanced raman spectroscopy: Detection platforms for sample analysis with and without electrophoretic separation
In this work we present two platforms of relatively simple construction for user-friendly sample detection via surface enhanced Raman spectroscopy (SERS). The first platform is designed for direct ...
Photon-upconverting nanoparticles as a novel background-free label in immunoassays
Farka, Z.; Hlaváček, Antonín; Mickert, M. J.; Skládal, P.; Gorris, H. H.
2018 - anglický
Photon-upconverting nanoparticles (UCNPs) have become an attractive label in immunoassays because their anti-Stokes luminescence can be excited by the NIR laser and detected in the VIS region without optical background interference. Further advantages of UCNPs include good photostability, large anti-Stokes shifts, and multiple narrow emission bands that can be used for multiplexed detection. We have developed a competitive upconversion-linked immunosorbent assay (ULISA) for detection of the pharmaceutical diclofenac (DCF) in surface waters. Silica-coated UCNPs (50 nm in diameter) with carboxyl groups on the surface were synthesized and conjugated with the secondary anti-IgG antibody. The structure and monodispersity of the nanoconjugates was studied by TEM and agarose gel electrophoresis. Using a highly affine anti-DCF primary antibody, the optimized ULISA provided a detection limit of 50 pg·mL−1.
Klíčová slova:
photon-upconversion nanoparticle; immunoassay; bioconjugation; antibody; diclofenac
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Photon-upconverting nanoparticles as a novel background-free label in immunoassays
Photon-upconverting nanoparticles (UCNPs) have become an attractive label in immunoassays because their anti-Stokes luminescence can be excited by the NIR laser and detected in the VIS region without ...
Determination of permeability of ulra-fine lead oxide aerosols through military filters
Kellnerova, E.; Večeřa, Zbyněk; Kellner, J.; Zeman, T.; Hylak, Č.
2018 - anglický
Military filters introduced in the Army of the Czech Republic are most commonly used when working with combat equipment and weapons and in manoeuvring activities in the terrain with the use of explosives or chemical warfare agents. During such activities, a large number of pollutants in the form of nanoparticles are released. Nanoparticles, irrespective of their chemical composition, are classified as carcinogenic to humans and therefore it is necessary to eliminate them from the air. Military filters, especially the combined ones, which contain a filtration as well as sorption component, are most widely used and are designed for the widest range of pollutants. However, the current methodology to evaluate the effectiveness of military filters does not fix a duty to monitor the ability of the filters to capture relevant pollutants across the whole range of sizes. The paper evaluates the efficiency of selected types of military filters using the methodology and instrumentation introduced in the accredited laboratories of the Institute of Analytical Chemistry of the Academy of Sciences of the Czech Republic. The testing has been carried out simultaneously with two concentrations of ultra-fine aerosols containing lead oxide nanoparticles ranging in size from 7.6 nm to 299.6 nm. It is an aerosol, the physicochemical and toxicological properties of which are known. During the work the basic parameters of permeability of aerosols tested by filters have been evaluated, especially: size and number of particles in front of and behind the filter, the efficiency and penetration of nanoparticles by the filter.
Klíčová slova:
nanoparticle; filter; efficiency; health safety
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Determination of permeability of ulra-fine lead oxide aerosols through military filters
Military filters introduced in the Army of the Czech Republic are most commonly used when working with combat equipment and weapons and in manoeuvring activities in the terrain with the use of ...
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