Quantitative detection of mRNA in tobacco
Štorchová, Helena; Libus, Jiří; Vaňková, Radomíra
2004 - English Keywords: Quantitative RT PCR; optimization; tobacco Available at various institutes of the ASCR Quantitative detection of mRNA in tobacco

Štorchová, Helena; Libus, Jiří; Vaňková, Radomíra
Ústav experimentální botaniky, 2004

24-Epibrassinolide at Subnanomolar Concentrations Modulates Growth of Mouse Hybridoma Cells
Franěk, František; Kohout, Ladislav; Eckschlager, T.
2003 - English Brassinolides are known to stimulate plant growth and to posses antistresiTactivities in plants. This work was aimed at exploring possible beneficial effects of 24-epibrassinolide on cultured mammalian cells. A mouse hybridoma, i.e. a hybrid ymphocyte cell line, was cultured either in standard serum-free medium, or in diluted medium in which the cell grew inder nutritional stress. Steady-state parameters of semicon-finuous cultures conducted at 24-epibrassinolide concentra-ions from 10~16 M to 1CT9 M were evaluated. Typical effects of he agent that were observed both in standard and in diluted i [media were suppression of intracellular antibody level, increa- / ,ed value of mitochondrial membrane potential, higher frac- / ion of cells in the G(/Gl phase and lower fraction of cells in / he S phase. Alleviation of nutritional stress was observed in j (cultures conducted in diluted media. Viable cell density was j ignificantly higher at 24-epibrassinolide concentrations 10~13[ knd 10~12 M. Results of this exploratory study show that thej plant hormone 24-epibrassinolide may induce alterations of he cell division mechanism of the energetic metabolism, and of secreted protein synthesis in a mammalian cell line. Keywords: Brassinolides; cell division Available at various institutes of the ASCR 24-Epibrassinolide at Subnanomolar Concentrations Modulates Growth of Mouse Hybridoma Cells

Brassinolides are known to stimulate plant growth and to posses antistresiTactivities in plants. This work was aimed at exploring possible beneficial effects of 24-epibrassinolide on cultured ...

Franěk, František; Kohout, Ladislav; Eckschlager, T.
Ústav experimentální botaniky, 2003

Preparation of Plant cDNA Libraries Using SMART Technology (CLONTECH)
Štorchová, Helena
2003 - Czech Keywords: preparation; cDNA; plant Available at various institutes of the ASCR Preparation of Plant cDNA Libraries Using SMART Technology (CLONTECH)

Štorchová, Helena
Ústav experimentální botaniky, 2003

Microbial Degradation of TCA in Norway Spruce
Forczek, Sándor; Matucha, Miroslav; Gryndler, Milan; Albrechtová, Jolana
2003 - English Keywords: biodegradation; chloroacetic acid; phyllosphere Available at various institutes of the ASCR Microbial Degradation of TCA in Norway Spruce

Forczek, Sándor; Matucha, Miroslav; Gryndler, Milan; Albrechtová, Jolana
Ústav experimentální botaniky, 2003

Construction of chromosome-specific BAC libraries in plants
Janda, Jaroslav; Šafář, Jan; Bartoš, Jan; Doleželová, Marie; Kovářová, Pavlína; Valárik, Miroslav; Čihalíková, Jarmila; Šimková, Hana; Doležel, Jaroslav
2003 - English Keywords: flow sorted chromosomes; BAC librares; wheat Available at various institutes of the ASCR Construction of chromosome-specific BAC libraries in plants

Janda, Jaroslav; Šafář, Jan; Bartoš, Jan; Doleželová, Marie; Kovářová, Pavlína; Valárik, Miroslav; Čihalíková, Jarmila; Šimková, Hana; Doležel, Jaroslav
Ústav experimentální botaniky, 2003

Analysis of plant genomes using flow cytometry
Bartoš, Jan; Doleželová, Marie; Šafář, Jan; Janda, Jaroslav; Kovářová, Pavlína; Čihalíková, Jarmila; Valárik, Miroslav; Šimková, Hana; Doležel, Jaroslav
2003 - English Keywords: flow cytometry; flow sorted chromosomes; FISH Available at various institutes of the ASCR Analysis of plant genomes using flow cytometry

Bartoš, Jan; Doleželová, Marie; Šafář, Jan; Janda, Jaroslav; Kovářová, Pavlína; Čihalíková, Jarmila; Valárik, Miroslav; Šimková, Hana; Doležel, Jaroslav
Ústav experimentální botaniky, 2003

Symptoms of damage of forest trees influenced by abiotic stress
Uhlířová, H.; Novotný, R.; Matucha, Miroslav
2003 - Czech Keywords: forest ecosystem; coniferous species; pollutants Available at various institutes of the ASCR Symptoms of damage of forest trees influenced by abiotic stress

Uhlířová, H.; Novotný, R.; Matucha, Miroslav
Ústav experimentální botaniky, 2003

Vliv TCA na smrk ztepilý
Forczek, Sándor; Albrechtová, Jolana; Matucha, Miroslav; Uhlířová, H.
2003 - Czech Keywords: TCA Available at various institutes of the ASCR Vliv TCA na smrk ztepilý

Forczek, Sándor; Albrechtová, Jolana; Matucha, Miroslav; Uhlířová, H.
Ústav experimentální botaniky, 2003

Documentation of some botanical objects by table scanners
Babůrek, Ivan
2002 - Czech Table scanners may be used for documentation of biological, and especially flat botanical, objects in laboratory. In comparison with (digital) photography is picture from scanner mostly cheaper and quicker. In comparison with classical botany herbaria picture better catch the shape and colour of fresh biological object. Small 3D objects (seeds, fruits) may be also scanned without problems. Resolution 600 dpi is higher than that of human eye. Scanned picture may be used instead of using small magnifying glass. Stolní skenery mohou být využity pro dokumentaci biologických, a zvláště botanických, plochých objektů v laboratoři stejně dobře, jako (digitální) fotodokumentace. Lépe než herbářové položky zachycují tvar a barevnost, oproti fotografické dokumentaci jsou většinou rychlejší a levnější. Drobné trojrozměrné předměty (plody, semena) lze dokumentovat také. Obvyklé rozlišení 600 dpi je již vyšší než rozlišení lidského oka a může nahradit použití lupy při pozorování objektu. Keywords: botany; dokumentation; scanner; botanika Available at various institutes of the ASCR Documentation of some botanical objects by table scanners

Table scanners may be used for documentation of biological, and especially flat botanical, objects in laboratory. In comparison with (digital) photography is picture from scanner mostly cheaper and ...

Babůrek, Ivan
Ústav experimentální botaniky, 2002

Approaches to elucidating the role of subcellular compartmentation in the regulation of cytokinin metabolism
Kiran, N.; Genkov, T.; Malbeck, Jiří; Brzobohatý, Břetislav
2002 - English Keywords: cytokinins; subcellular compartmentation; Nicotiana tabacum Available at various institutes of the ASCR Approaches to elucidating the role of subcellular compartmentation in the regulation of cytokinin metabolism

Kiran, N.; Genkov, T.; Malbeck, Jiří; Brzobohatý, Břetislav
Ústav experimentální botaniky, 2002