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Microbial preparation of intracellular oxidoreductases for food purposes
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2015 - English
Development and optimization of pilot cultivations and DSP of induced intracellular oxidoreductases microbial productions.\n\n
Keywords:
enzyme production
Available at various institutes of the ASCR
Microbial preparation of intracellular oxidoreductases for food purposes
Development and optimization of pilot cultivations and DSP of induced intracellular oxidoreductases microbial productions.\n\n
Vývoj technologie pilotní přípravy chitinázy
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2014 - English
Development and optimization of the production of extracellular chitinase by the natural producer and also by recombinant host. Cultivation technology and down-stream processing.
Keywords:
enzyme production; bacterial production; submergeed aerobic cultivation
Available at various institutes of the ASCR
Vývoj technologie pilotní přípravy chitinázy
Development and optimization of the production of extracellular chitinase by the natural producer and also by recombinant host. Cultivation technology and down-stream processing.
Seeking Novel Nitrilase Enzyme (Nitrile Aminohydrolase, EC 3.5.5.1)
Martínková, Ludmila; Kaplan, Ondřej; Veselá, Alicja Barbara
2014 - English
Our research group has created a library of novel nitrilases. In general, the enzymes are available from heterologous producers in amounts ranging from 10 to 20 mg of protein exhbiting 25-50 % purity. The enzymes differ from each other in substrate specificity, temperature optimum, enantioselectivity (arylacetonitrilases) etc. Preferential substrates are phenylacetonitrile, (R,S)-mandelonitrile, benzonitrile or cyanopyridines (depending on the enzyme).
Keywords:
Nitrilase; Expression; Purification
Available at various institutes of the ASCR
Seeking Novel Nitrilase Enzyme (Nitrile Aminohydrolase, EC 3.5.5.1)
Our research group has created a library of novel nitrilases. In general, the enzymes are available from heterologous producers in amounts ranging from 10 to 20 mg of protein exhbiting 25-50 % purity. ...
Vývoj technologií lyopfilizace buněčných lyzátů
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2014 - English
Development and optimization of lyophilization methods of the cell content avoiding loss of functionality.
Keywords:
lyophyllization; cell content; biotechnology
Available at various institutes of the ASCR
Vývoj technologií lyopfilizace buněčných lyzátů
Development and optimization of lyophilization methods of the cell content avoiding loss of functionality.
Microbial and molecular biological analysis of technological solutions from Glanzstoff Bohemia s.r.o
Brennerová, Mária
2014 - English
Main goal of the study was identification of the bacterial contaminations during the production of high-tenacity viscose filaments. The work describes microbial cultivations, isolation of bacteria from technological solutions, and taxonomical identification through sequencing of 16S ribosomal RNA genes and searching for affiliations with bacterial relatives in the NCBI website.
Keywords:
viscose; technological solutions; bacterial isolates; identification
Available at various institutes of the ASCR
Microbial and molecular biological analysis of technological solutions from Glanzstoff Bohemia s.r.o
Main goal of the study was identification of the bacterial contaminations during the production of high-tenacity viscose filaments. The work describes microbial cultivations, isolation of bacteria ...
Vývoj kultivačních technologií výroby intracelulárních alkoholdehydrogenáz
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2014 - English
Development and optimization of pilot cultivations and DSP of intracellular recombinant enzyme productions.
Keywords:
enzyme production; bacterial production; submergeed aerobic cultivation
Available at various institutes of the ASCR
Vývoj kultivačních technologií výroby intracelulárních alkoholdehydrogenáz
Development and optimization of pilot cultivations and DSP of intracellular recombinant enzyme productions.
Host interactions and lactobacilli: from fly model to mouse model/ Role of the intestinal lactobacilli in growth promoting in juvenile development
Kozáková, Hana
2014 - English
We confirmed the hypothesis that the intestinal flora supports juvenile growth in mice, and that the bacterial isolate is capable and mice recapitulate promote growth, similarly as complete microflora.
Keywords:
flies; lactobacili; mice
Available at various institutes of the ASCR
Host interactions and lactobacilli: from fly model to mouse model/ Role of the intestinal lactobacilli in growth promoting in juvenile development
We confirmed the hypothesis that the intestinal flora supports juvenile growth in mice, and that the bacterial isolate is capable and mice recapitulate promote growth, similarly as complete ...
Transfer of technology for production of AEH from Sphingomonas sp
Kyslíková, Eva; Palyzová, Andrea; Marešová, Helena; Štěpánek, Václav; Zahradník, Jiří; Bečka, Stanislav; Dobišová, Marie; Kyslík, Pavel
2014 - English
Research report on construction of a recombinant production microorganism, the way of its cultivation in stirred bioreactor, overproduction and isolation of AEH and stabilization of AEH as cross linked enzyme agregate
Keywords:
Alpha aminoacid ester hydrolase; heterologous expression; stirred bioreactor
Available at various institutes of the ASCR
Transfer of technology for production of AEH from Sphingomonas sp
Research report on construction of a recombinant production microorganism, the way of its cultivation in stirred bioreactor, overproduction and isolation of AEH and stabilization of AEH as cross ...
Vývoj technologií pilotní přípravy laktamáz a dalších enzymů
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2014 - English
Development and optimization of the production of some enzymes for pharmaceutical industry. Cultivation technology and down-stream processing.
Keywords:
enzyme production; bacterial production; submerged aerobic cultivation; biotechnology
Available at various institutes of the ASCR
Vývoj technologií pilotní přípravy laktamáz a dalších enzymů
Development and optimization of the production of some enzymes for pharmaceutical industry. Cultivation technology and down-stream processing.
Development of recombinant Pichia pastoris strain expressing synthetic gene of npga by genetic cloning
Marešová, Helena; Maršálek, Jaroslav; Bečka, Stanislav; Kyslík, Pavel
2012 - English
Cloning of synthetic npga gene into Pichia pastoris. Development of high expression system for penicillin acylase in a stirred bioreactor. Isolation and characterization of the enzyme.
Keywords:
penicillin acylase; heterologous expression; stirred bioreactor
Available at various institutes of the ASCR
Development of recombinant Pichia pastoris strain expressing synthetic gene of npga by genetic cloning
Cloning of synthetic npga gene into Pichia pastoris. Development of high expression system for penicillin acylase in a stirred bioreactor. Isolation and characterization of the enzyme.
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