Study of aggregated forms of proteinase inhibitors from boar seminl plasma by gel chromatography
Jelínková, Petra; Maňásková, Pavla; Tichá, M.; Jonáková, Věra
2002 - English
Most of proteins in seminal plasma exist under physiological conditions in the form of aggregates. The study of the presence of proteinase inhibitosr in different associated forms of boar seminal plasma proteins is the subject of our present communication.
Keywords:
Gel chromatography; proteinase inhibitors; seminal plasma
Available at various institutes of the ASCR
Study of aggregated forms of proteinase inhibitors from boar seminl plasma by gel chromatography
Most of proteins in seminal plasma exist under physiological conditions in the form of aggregates. The study of the presence of proteinase inhibitosr in different associated forms of boar seminal ...
Sperm surface proteins of spermatozoa and proteins of seminal plasma in fertilization
Jonáková, Věra; Maňásková, Pavla; Jelínková, Petra; Balínová, P.; Tichá, M.
2002 - Czech
Mammalian fertilization involves highly regulated biochemical events. We aimed at study of interaction of seminal plasma proteins with sperm and at mutual interactions of proteins from seminal plasma. Savčí fertilizace zahrnuje vysoce regulované biochemické děje. My jsme se zaměřili na studium interakce proteinů semenné plasmy se spermií a na vzájemné interakce proteinů ze semenné plasmy.
Keywords:
fertilization; proteins of seminal plasma; spermatozoa; hnojení
Available at various institutes of the ASCR
Sperm surface proteins of spermatozoa and proteins of seminal plasma in fertilization
Mammalian fertilization involves highly regulated biochemical events. We aimed at study of interaction of seminal plasma proteins with sperm and at mutual interactions of proteins from seminal ...
Serine proteinase inhibitors isolated from boar seminal plasma
Jelínková, Petra; Maňásková, Pavla; Tichá, M.; Jonáková, Věra
2002 - English
Serine proteinase inhibitors were found in boar seminal plasma. The work was concentrated on isolation and characterization of proteinase inhibitors and study of the existence of inhibitors in complexes with other proteins in seminal plasma.
Keywords:
proteinase inhibitors; seminal plasma; proteins; bílkoviny
Available at various institutes of the ASCR
Serine proteinase inhibitors isolated from boar seminal plasma
Serine proteinase inhibitors were found in boar seminal plasma. The work was concentrated on isolation and characterization of proteinase inhibitors and study of the existence of inhibitors in ...
Protein interactions involved in the formation of aggregated forms of boar seminal plasma proteins
Maňásková, Pavla; Balínová, P.; Tichá, M.; Jonáková, Věra
2002 - English
Mutual interactions of isolated proteins were tested by means of gel chromatography. The results obtained confirmed an existence of interactions between spermadhesins of the AQN and AWN families as well as between proteins involved in the formation of heterodimer PSPI/PSPII.
Keywords:
spermadhesins; aggregates; aggregated forms
Available at various institutes of the ASCR
Protein interactions involved in the formation of aggregated forms of boar seminal plasma proteins
Mutual interactions of isolated proteins were tested by means of gel chromatography. The results obtained confirmed an existence of interactions between spermadhesins of the AQN and AWN families as ...
Effect of endocrine disruptors on the microtubules of MCF-7 cells
Pěknicová, Jana; Kubátová, Alena; Kyselová, Vendula; Buckiová, Daniela; Paleček, J.; Dráber, Pavel
2002 - English
Keywords:
microtubules; endocrine disruptor; MCF-7 cell
Available at various institutes of the ASCR
Effect of endocrine disruptors on the microtubules of MCF-7 cells
Cryocrystallography of biological Macromolecules
Řezáčová, Pavlína
2002 - English
Crystals of biological macromolecules at near to room temperature are sensitive to X-rays and frequently suffer from radiation damage, especially when X-ray experiments are carried out on highly intense synchrotron beamlines. Performing such experiments at cryogenic temperatures greatly reduce, or eliminate radiation damage and thus produce higher quality diffraction data.
Keywords:
cryocrystallography; radiation demage; protein crystals
Available at various institutes of the ASCR
Cryocrystallography of biological Macromolecules
Crystals of biological macromolecules at near to room temperature are sensitive to X-rays and frequently suffer from radiation damage, especially when X-ray experiments are carried out on highly ...
Proteins from boar prostate secretion: their isolation and characterization
Maňásková, Pavla; Ryšlavá, H.; Tichá, M.; Jonáková, Věra
2002 - English
Prostate proteins were separated by affinity chromatography on immobilized heparin. Major identified components of prostate secretion:beta-microseminoprotein, serotransferin, serum albumin, PSP I and II spermadhesins.
Keywords:
prostate; accessory sexual glands; boar
Available at various institutes of the ASCR
Proteins from boar prostate secretion: their isolation and characterization
Prostate proteins were separated by affinity chromatography on immobilized heparin. Major identified components of prostate secretion:beta-microseminoprotein, serotransferin, serum albumin, PSP I and ...
Gamma-tubulin and its function
Dráber, Pavel
2002 - English
Keywords:
gamma-tubulin; microtubules; nucleation
Available at various institutes of the ASCR
Gamma-tubulin and its function
Use of RT-PCR for detection of cytokine gene expression and induciblenitric-oxide synthase in cornea grafts
Rychnavská, Z.; Sedláková, K.; Plšková, Jarmila; Filipec, M.; Holáň, Vladimír
2002 - Czech
Expression of the genes for cytokines and other immunoregulatory molecules inrejected cornea xenografts was detected by means of RT-PCR. Exprese genů pro cytokiny a další imunoregulační molekuly byla detekována vxenotransplantátech rohovky pomocí RT-PCR.
Keywords:
nitric oxide; cytokines
Available at various institutes of the ASCR
Use of RT-PCR for detection of cytokine gene expression and induciblenitric-oxide synthase in cornea grafts
Expression of the genes for cytokines and other immunoregulatory molecules inrejected cornea xenografts was detected by means of RT-PCR.
An approach for isolation of human seminal plasma proteins
Kraus, Marek; Tichá, M.; Jonáková, Věra
2002 - English
Human seminal plasma contains various type of protein. For their isolation were used these methods: affinity chromatography, gel chromatography, HPLC etc. The cross-reactivity of antibodies against boar seprmadhesing AQN and AWN was studied with human seminal proteins.
Keywords:
human seminal plasma; gel chromatography; 2.D-electrophoresis
Available at various institutes of the ASCR
An approach for isolation of human seminal plasma proteins
Human seminal plasma contains various type of protein. For their isolation were used these methods: affinity chromatography, gel chromatography, HPLC etc. The cross-reactivity of antibodies against ...
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