BIOGENIC AND SYNTHETIC AMORPHOUS SILICA - COMPARE AND INTERACTION WITH BACTERIAL SYSTEMS
Fijalkowski, M.; Adach, K.; Marešová, Helena; Kroisová, D.
2015 - English
The aim of the work is to compare the biogenic silica nanoparticles, which have been isolated from rice husks with synthetically produced silica brand Cab-O-Sil LM-150. The comparison is based on an evaluation of the two systems from the point of view of chemical composition, particle size and structure, ability to form clusters of particles and interactions with selected bacterial systems. Methods used for comparison were: SEM, EDX, TEM, FTIR, ICPOES and bacteriological tests. Husks are standardly contaminated by accompanying ions that are important for plant growth, but undesirable for obtaining a quality product. Rice husk used for the silica isolation were purified by boiling in 10% HCl for 2 hours. Rice husks were then washed with distilled water until pH 7. The dried and purified husks were burned in an oven at 650 degrees C of ramping temperature 10 degrees C/min during 2 hours. The obtained product has a chemical composition analogous to the synthetic product. Biogenic particles reach a size of about 20 nm, about 10 nm synthetic. Both materials have an amorphous structure. Interaction with bacterial systems was performed with Gram-negative bacteria Escherichia coli W3110 strain and Gram-positive bacteria Bacillus cereus. In both cases analogous behaviour was observed depending on the concentration of nanoparticles and reducing the growth rate of cultures of 8% for E. coli and 5% for B. cereus resulted nanoparticles at the concentration of 150 mg/litter of medium.
Keywords:
Biogenic silica; synthetic silica; chemical composition
Available at various institutes of the ASCR
BIOGENIC AND SYNTHETIC AMORPHOUS SILICA - COMPARE AND INTERACTION WITH BACTERIAL SYSTEMS
The aim of the work is to compare the biogenic silica nanoparticles, which have been isolated from rice husks with synthetically produced silica brand Cab-O-Sil LM-150. The comparison is based on an ...
Production of sample of testing siderophore preparation
Palyzová, Andrea; Kyslík, Pavel
2015 - Czech
Description of batch culture process of fur mutant of wild strain Pseudomonas aeruginosa PAO1 for siderophores production in continuously stirred bioreactor and preparation of lyophilized mixed siderophores (pyoverdin and pyochelin) sample. Popis průběhu jednorázové kultivace fur mutanta kmene Pseudomonas aeruginosa PAO1 pro produkci sideroforů v míchaném bioreaktoru a příprava lyofilizovaného vzorku směsi sideroforů (pyoverdin a pyochelin).
Keywords:
batch culture; siderophores; Pseudomonas aeruginosa
Available at various institutes of the ASCR
Production of sample of testing siderophore preparation
Description of batch culture process of fur mutant of wild strain Pseudomonas aeruginosa PAO1 for siderophores production in continuously stirred bioreactor and preparation of lyophilized mixed ...
Development of the cultivation technology of the recombinant yeast Pichia pastoris for enzymes' preparation.
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2015 - Czech
Development and optimization of the cultivation technology of the methylotrophic yeast Pichia pastoris for the preparation of enzymes serving to the treatment of feed mixtures of piglets. Vývoj a optimalizace kultivační technologie metylotrofní kvasinky Pichia pastoris pro přípravu enzymů pro úpravu krmných směsí selat.
Keywords:
Pichia production
Available at various institutes of the ASCR
Development of the cultivation technology of the recombinant yeast Pichia pastoris for enzymes' preparation.
Development and optimization of the cultivation technology of the methylotrophic yeast Pichia pastoris for the preparation of enzymes serving to the treatment of feed mixtures of piglets.
Development of pilot production of inactivated bacterial lysates
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Urban, M.
2015 - Czech
Development and optimization of the pilot production of inactivated bacterial lysates for the production of dietary supplements Vývoj a optimalizace pilotní přípravy inaktivovaných bakteriálních lyzátů pro přípravu potravinových doplňků.
Keywords:
bacterial production
Available at various institutes of the ASCR
Development of pilot production of inactivated bacterial lysates
Development and optimization of the pilot production of inactivated bacterial lysates for the production of dietary supplements
Preparation of inactivated bacterial biomass.
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav
2015 - Czech
Development and optimization of the pilot production of inactivated bacterial biomass for the production of dietary supplements. Vývoj a optimalizace pilotní přípravy inaktivované bakteriální biomasy pro přípravu potravinových doplňků.
Keywords:
bacterial production
Available at various institutes of the ASCR
Preparation of inactivated bacterial biomass.
Development and optimization of the pilot production of inactivated bacterial biomass for the production of dietary supplements.
Vývoj technologie pilotní přípravy chitinázy
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2014 - English
Development and optimization of the production of extracellular chitinase by the natural producer and also by recombinant host. Cultivation technology and down-stream processing.
Keywords:
enzyme production; bacterial production; submergeed aerobic cultivation
Available at various institutes of the ASCR
Vývoj technologie pilotní přípravy chitinázy
Development and optimization of the production of extracellular chitinase by the natural producer and also by recombinant host. Cultivation technology and down-stream processing.
Conservation of bioinsecticides by drying
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2014 - Czech
Downstream processing of biological insecticide agents, spray drying, preservation of biological efficiency. Downstream processing biologických insekticidních přípravků, sušení sprayováním při zachování biologické účinnosti.
Keywords:
downstream processing; spray drying
Available at various institutes of the ASCR
Conservation of bioinsecticides by drying
Downstream processing of biological insecticide agents, spray drying, preservation of biological efficiency.
Seeking Novel Nitrilase Enzyme (Nitrile Aminohydrolase, EC 3.5.5.1)
Martínková, Ludmila; Kaplan, Ondřej; Veselá, Alicja Barbara
2014 - English
Our research group has created a library of novel nitrilases. In general, the enzymes are available from heterologous producers in amounts ranging from 10 to 20 mg of protein exhbiting 25-50 % purity. The enzymes differ from each other in substrate specificity, temperature optimum, enantioselectivity (arylacetonitrilases) etc. Preferential substrates are phenylacetonitrile, (R,S)-mandelonitrile, benzonitrile or cyanopyridines (depending on the enzyme).
Keywords:
Nitrilase; Expression; Purification
Available at various institutes of the ASCR
Seeking Novel Nitrilase Enzyme (Nitrile Aminohydrolase, EC 3.5.5.1)
Our research group has created a library of novel nitrilases. In general, the enzymes are available from heterologous producers in amounts ranging from 10 to 20 mg of protein exhbiting 25-50 % purity. ...
Gut microbiome and colorectal cancer
Tlaskalová-Hogenová, Helena; Klimešová, Klára; Zákostelská, Zuzana; Kverka, Miloslav; Hornová, Michaela; Vannucci, Luca; Štěpánková, Renata; Hudcovic, Tomáš; Kozáková, Hana; Rossmann, Pavel
2014 - English
The work deals with metagenomic approaches that are currently being used to decipher the genome of the microbiota (microbiome), and, in parallel, functional studies are being performed to analyze the effects of microbiota on the host.
Keywords:
gut; colorectal; cancer
Available at various institutes of the ASCR
Gut microbiome and colorectal cancer
The work deals with metagenomic approaches that are currently being used to decipher the genome of the microbiota (microbiome), and, in parallel, functional studies are being performed to analyze the ...
DNA sequencing
Felsberg, Jürgen
2014 - Czech
Performance of scientific and technical work largely consisting of DNA sequencing according to client specification. In adition all DNA analysis necessery for DNA sequencing are carried out. Provedení vědecko-technických prací, spočívajících v sekvenování DNA podle zadání objednatele. Dále jsou prováděny všechny potřebné analýzy nukleových kyselin nutné k sekvenování DNA.
Keywords:
DNA sequencing
Available at various institutes of the ASCR
DNA sequencing
Performance of scientific and technical work largely consisting of DNA sequencing according to client specification. In adition all DNA analysis necessery for DNA sequencing are carried out.
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