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Počet nalezených dokumentů:
509
Publikováno od
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Nanodiamonds as an innovative system for intracellular delivery of mirna-34a inprostatic cancer therapy
Bitti, G.; Abate, M.; Neuhoferová, Eva; Kindermann, Marek; Petráková, V.; Boccellino, M.; Quagliuolo, L.; Filová, Eva; Benson, Veronika; Caraglia, M.; Amler, Evžen
2018 - anglický
The microRNA(miRNA)-34a is an important regulator of tumor suppression. It controls the expression of several target proteins involved in cell cycle, differentiation and apoptosis, and antagonizes processes that are necessary for basic cancer cell viability as well as cancer stemness, metastasis, and chemoresistance. It is downregulated in numerous cancer types, including prostatic cancer, and inhibits malignant growth by repressing genes involved in various oncogenic signaling pathways. Given the anti-oncogenic activity of miR-34a, here we proved the substantial benefits of a new therapeutic concept based on nanotechnology delivery of miRNA mimics. In order to monitor the miRNA-34a replacement, we used a fluorescent nanodiamond particles (FND) system with linked miRNA-34a mimic, which was delivered to PC3 and DU145 prostatic cancer cell lines. We used functionalized nanodiamonds coated with polyethylenimine to transfer miRNA-34a into PC3 and DU145 prostatic cancer cell lines and we measured the zeta-potential of these complexes before using them for in vitro experiments. A replacement of miRNA-34 was observed by monitoring levels of miRNA-34 via real-time PCR. Moreover, our in vitro experiments demonstrated that miRNA-34a replacement, using this FND delivery system, decreased viability and induced apoptosis in prostatic cancer cell lines. Our findings suggest the replacement of oncosuppressor miRNA-34a provides an effective strategy for cancer therapy and the FND-based delivery systems seems to be an excellent strategy for a safe and effective targeting of the tumor.
Klíčová slova:
miR-34a; nanodiamonds; prostate cancer
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Nanodiamonds as an innovative system for intracellular delivery of mirna-34a inprostatic cancer therapy
The microRNA(miRNA)-34a is an important regulator of tumor suppression. It controls the expression of several target proteins involved in cell cycle, differentiation and apoptosis, and antagonizes ...
Effect of nanodiamond particles on neuronal tissue
Šimková, Veronika; Benson, Veronika
2018 - anglický
High-Pressure High-Temperature nanodiamonds are considered to be highly biocompatible due to\ntheir low acute toxicity, which makes them suitable for biomedical applications. However, next to interaction with the cell membrane, nanoscale allows particles to penetrate the plasma membrane and even accumulate in the cell cytoplasm. Recently it brought up questions regarding sustainable nanoparticle application in vivo and potential toxicity hazards. The neuronal tissue consists of neurons and glial cells, and it is separated by a bloodbrain barrier from the peripheral blood system. Neurons are nerve cells forming a complex network of synapses that carry signals in the brain. The neurons are nourished and protected by glial cells. This project aims to find out whether nanodiamonds pass the blood-brain barrier when applied intravenously as a drug carrier and whether they directly interact with the neuronal tissue.
Klíčová slova:
nanodiamonds; cell cytoplasm; neuronal tissue
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Effect of nanodiamond particles on neuronal tissue
High-Pressure High-Temperature nanodiamonds are considered to be highly biocompatible due to\ntheir low acute toxicity, which makes them suitable for biomedical applications. However, next to ...
Development and optimization of the production of recombinant epimerases for pharmaceutical industry. Scale-up of a submerged cultivation technology and down-stream processing
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
2017 - anglický
Development and optimization of the production of recombinant epimerases for pharmaceutical industry. Scale-up of a submerged cultivation technology and down-stream processing.
Klíčová slova:
enzyme production
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Development and optimization of the production of recombinant epimerases for pharmaceutical industry. Scale-up of a submerged cultivation technology and down-stream processing
Development and optimization of the production of recombinant epimerases for pharmaceutical industry. Scale-up of a submerged cultivation technology and down-stream processing.
Scale-up and production of various recombinant oxidoreductases
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2017 - anglický
Development and optimization of pilot cultivations and DSP of recombinant intracellular oxidoreductases in microbial productions for a food industrial use
Klíčová slova:
enzyme production
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Scale-up and production of various recombinant oxidoreductases
Development and optimization of pilot cultivations and DSP of recombinant intracellular oxidoreductases in microbial productions for a food industrial use
UNICELLULAR ALGA CHLORELLA AS A FEED SUPPLEMENT, CURRENT EXPERIENCE AND FUTURE PERSPECTIVES
Kotrbáček, V.; Doucha, Jiří; Kuchařová, V.; Doubek, J.
2017 - anglický
We studied the effect of Chlorella biomass as a feed supplement. In laying hens we found that diet supplemented with 1 % (i.e. 10 g/kg) of dry disintegrated heterotrophically grown Chlorella biomass significantly increased content of carotenoids in egg yolks of 8 experimental animals already after the first week of supplementation (P<0,01). Compared to 8 control hens these differences remained significantly higher until the end of the eight-week experiment. The average carotenoid concentration was 17,3 mu g per g of yolk in control group and 25,3 mu g per g of yolk in experimental animals (P<0,01). Autotrophically grown Chlorella was supplemented to the 30 gilts one month prior the expected first heat in a dose of 1,5 g per animal per day. These sows entered heat earlier and the number of pregnancy was higher in comparison to the 30 control animals. Other studies observed digestibility of selenium organically bound in Se-enriched Chlorella. The level of selenium in blood, colostrum and also tissues of new born piglets was determined. Group of 8 sows was supplemented with Se-enriched alga during the pregnancy and 3 days after parturition at a dose of 0,3 mg of selenium per 1 kg of feed. The same amount of selenium in the form of sodium selenite was administrated to the control group of 8 individuals. Organic form of selenium significantly increased concentration of Se in blood of sows and colostrum (P<0,05). Piglets born to supplemented sows had also significantly higher level of Se in heart, muscles and liver (P<0.01). The use of fresh Chlorella biomass produced outdoors on a dairy farm was experimentally tested. The liquid fresh biomass applied to dairy cows during the pregnancy at a dose of 15 g dry mass per cow increased the dry matter content in colostrum from 17,5 g per 100 ml of colostrum in control group to 20,5 g per 100 ml of colostrum in supplemented cows (P<0,05).
Klíčová slova:
Chlorella; feed supplement; flue gas utilization
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
UNICELLULAR ALGA CHLORELLA AS A FEED SUPPLEMENT, CURRENT EXPERIENCE AND FUTURE PERSPECTIVES
We studied the effect of Chlorella biomass as a feed supplement. In laying hens we found that diet supplemented with 1 % (i.e. 10 g/kg) of dry disintegrated heterotrophically grown Chlorella biomass ...
Development of a fed-batch technology of the yeast P. pastoris recombinant protein production in a small pilot scale. Optimization of the process and preparation of a scale-up
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2017 - anglický
Development of a fed-batch technology of the yeast P. pastoris recombinant protein production in a small pilot scale. Optimization of the process and preparation of a scale-up.
Klíčová slova:
pastoris production
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Development of a fed-batch technology of the yeast P. pastoris recombinant protein production in a small pilot scale. Optimization of the process and preparation of a scale-up
Development of a fed-batch technology of the yeast P. pastoris recombinant protein production in a small pilot scale. Optimization of the process and preparation of a scale-up.
Inaktivovaná bakteriální biomasa pro přípravu lyzátů
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
2017 - český
Inaktivované bakteriální biomasy pro přípravu potravinových doplňků - optimalizace kultivací zdrojových kmenů.\n\n Inactivated bacterial biomass for the production of dietary supplements\n- optimization of the cultivation of the source strains.\n
Klíčová slova:
bacterial production
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Inaktivovaná bakteriální biomasa pro přípravu lyzátů
Inaktivované bakteriální biomasy pro přípravu potravinových doplňků - optimalizace kultivací zdrojových kmenů.\n\n...
Development of the cultivation of the pathogenic bacteria.
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
2017 - anglický
Development of the cultivation of pathogenic bacteria with a specific lipopolysaccharide content in the cell structures. Optimization of media and yields.\n\n
Klíčová slova:
cultivation of pathogens
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Development of the cultivation of the pathogenic bacteria.
Development of the cultivation of pathogenic bacteria with a specific lipopolysaccharide content in the cell structures. Optimization of media and yields.\n\n
Optimalizace a příprava výroby podpůrných přípravků pro ošetření slunečnice
Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Prell, Aleš; Vavruška, Tomáš
2017 - český
Příprava výroby a optimalizace výroby zemědělských pomocných přípravků pro podporu pěstování slunečnice Preparation of production and optimization of agriculture auxiliary fertilizers for a supported cultivation of sunflower..
Klíčová slova:
auxiliary fertilizers
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Optimalizace a příprava výroby podpůrných přípravků pro ošetření slunečnice
Příprava výroby a optimalizace výroby zemědělských pomocných přípravků pro podporu pěstování slunečnice...
Optimalizace kultivace hlenky Schizochytrium spp. v bioreaktoru.
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
2017 - český
Optimalizace média pro kultivace hlenky produkující DHA. Vývoj procesu, hledání optimálních kultivačních podmínek. Optimization of the medium for the slime mold cultivation for the DHA production. Development of the process, optimal cultivation conditions searching
Klíčová slova:
mold cultivation; DHA
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Optimalizace kultivace hlenky Schizochytrium spp. v bioreaktoru.
Optimalizace média pro kultivace hlenky produkující DHA. Vývoj procesu, hledání optimálních kultivačních podmínek....
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