Advances in flow cytogenetics of barley
Suchánková, Pavla; Kubaláková, Marie; Kovářová, Pavlína; Bartoš, Jan; Čihalíková, Jarmila; Endo, T. R.; Doležel, Jaroslav
2004 - English Analýza pomocí průtokového cytometru umožňuje klasifikaci izolovaných chromozómů podle obsahu DNA. Každý chromozóm, který může být pomocí průtokového cytometru identifikován, může být zároveň velkou rychlostí tříděn z populace všech chromozómů. U ječmene však může být tříděn pouze nejmenší chromozóm 1H. Nová strategie je založena na použití adičních linií pšenice-ječmen nesoucích v genomu pšenice chromozómová ramena ječmene ve formě telocentrických chromozómů. Tato strategie umožňuje třídění jakéhokoliv ze 14 chromozómových ramen ječmene. Flow cytogenetics is an attractive tool that facilitates genome mapping. Flow cytometric analysis permits classification of isolated chromosomes according to their DNA content As thousands of chromosomes are analysed in each sample, quantitative detection of structural and numerical chromosome changes is possible. Any chromosome that can be discriminated can also be sorted in large quantities and high purities for subsequent molecular analysis. Unfortunately, only the smallest chromosome 5(1H) can be discriminated and sorted in barley. This is due to similarity in relative DNA content among the chromosomes 1 - 4,6 and 7 (2H - 7H). Cytogenetic stocks facilitate sorting of those translocation chromosomes that differ in DNA content Even if the sorted translocation chromosomes were found useful for mapping DNA sequences to subchromosomal regions, their use remained limited. Here we describe a novel approach that facilitates sorting of any of the 14 barley chromosome arms. The strategy is based on using wheat-barley addition lines carrying barley chromosome arms (telocentric chromosomes). Furthermore, we will be able to sort subarm chromosomal segments that are generated in the wheat-barley chromosome addition lines by the gametocidal system. These advances make flow cytogenetics an attractive tool that can simplify barley genome mapping. A possibility to purify large quantities of individual chromosome arms and their segments opens avenues for targeted isolation of low-copy "genie" sequences, preparation of specific probes for screening EST arrays, HAPPY mapping, and construction of chromosome arm-specific BAG libraries. Keywords: Flow cytogenetics; barley; flow karytyping Available at various institutes of the ASCR Advances in flow cytogenetics of barley

Analýza pomocí průtokového cytometru umožňuje klasifikaci izolovaných chromozómů podle obsahu DNA. Každý chromozóm, který může být pomocí průtokového cytometru identifikován, může být zároveň velkou ...

Suchánková, Pavla; Kubaláková, Marie; Kovářová, Pavlína; Bartoš, Jan; Čihalíková, Jarmila; Endo, T. R.; Doležel, Jaroslav
Ústav experimentální botaniky, 2004

Quantitative detection of mRNA in tobacco
Štorchová, Helena; Libus, Jiří; Vaňková, Radomíra
2004 - English Keywords: Quantitative RT PCR; optimization; tobacco Available at various institutes of the ASCR Quantitative detection of mRNA in tobacco

Štorchová, Helena; Libus, Jiří; Vaňková, Radomíra
Ústav experimentální botaniky, 2004

24-Epibrassinolide at Subnanomolar Concentrations Modulates Growth of Mouse Hybridoma Cells
Franěk, František; Kohout, Ladislav; Eckschlager, T.
2003 - English Brassinolides are known to stimulate plant growth and to posses antistresiTactivities in plants. This work was aimed at exploring possible beneficial effects of 24-epibrassinolide on cultured mammalian cells. A mouse hybridoma, i.e. a hybrid ymphocyte cell line, was cultured either in standard serum-free medium, or in diluted medium in which the cell grew inder nutritional stress. Steady-state parameters of semicon-finuous cultures conducted at 24-epibrassinolide concentra-ions from 10~16 M to 1CT9 M were evaluated. Typical effects of he agent that were observed both in standard and in diluted i [media were suppression of intracellular antibody level, increa- / ,ed value of mitochondrial membrane potential, higher frac- / ion of cells in the G(/Gl phase and lower fraction of cells in / he S phase. Alleviation of nutritional stress was observed in j (cultures conducted in diluted media. Viable cell density was j ignificantly higher at 24-epibrassinolide concentrations 10~13[ knd 10~12 M. Results of this exploratory study show that thej plant hormone 24-epibrassinolide may induce alterations of he cell division mechanism of the energetic metabolism, and of secreted protein synthesis in a mammalian cell line. Keywords: Brassinolides; cell division Available at various institutes of the ASCR 24-Epibrassinolide at Subnanomolar Concentrations Modulates Growth of Mouse Hybridoma Cells

Brassinolides are known to stimulate plant growth and to posses antistresiTactivities in plants. This work was aimed at exploring possible beneficial effects of 24-epibrassinolide on cultured ...

Franěk, František; Kohout, Ladislav; Eckschlager, T.
Ústav experimentální botaniky, 2003

Preparation of Plant cDNA Libraries Using SMART Technology (CLONTECH)
Štorchová, Helena
2003 - Czech Keywords: preparation; cDNA; plant Available at various institutes of the ASCR Preparation of Plant cDNA Libraries Using SMART Technology (CLONTECH)

Štorchová, Helena
Ústav experimentální botaniky, 2003

Microbial Degradation of TCA in Norway Spruce
Forczek, Sándor; Matucha, Miroslav; Gryndler, Milan; Albrechtová, Jolana
2003 - English Keywords: biodegradation; chloroacetic acid; phyllosphere Available at various institutes of the ASCR Microbial Degradation of TCA in Norway Spruce

Forczek, Sándor; Matucha, Miroslav; Gryndler, Milan; Albrechtová, Jolana
Ústav experimentální botaniky, 2003

Construction of chromosome-specific BAC libraries in plants
Janda, Jaroslav; Šafář, Jan; Bartoš, Jan; Doleželová, Marie; Kovářová, Pavlína; Valárik, Miroslav; Čihalíková, Jarmila; Šimková, Hana; Doležel, Jaroslav
2003 - English Keywords: flow sorted chromosomes; BAC librares; wheat Available at various institutes of the ASCR Construction of chromosome-specific BAC libraries in plants

Janda, Jaroslav; Šafář, Jan; Bartoš, Jan; Doleželová, Marie; Kovářová, Pavlína; Valárik, Miroslav; Čihalíková, Jarmila; Šimková, Hana; Doležel, Jaroslav
Ústav experimentální botaniky, 2003

Analysis of plant genomes using flow cytometry
Bartoš, Jan; Doleželová, Marie; Šafář, Jan; Janda, Jaroslav; Kovářová, Pavlína; Čihalíková, Jarmila; Valárik, Miroslav; Šimková, Hana; Doležel, Jaroslav
2003 - English Keywords: flow cytometry; flow sorted chromosomes; FISH Available at various institutes of the ASCR Analysis of plant genomes using flow cytometry

Bartoš, Jan; Doleželová, Marie; Šafář, Jan; Janda, Jaroslav; Kovářová, Pavlína; Čihalíková, Jarmila; Valárik, Miroslav; Šimková, Hana; Doležel, Jaroslav
Ústav experimentální botaniky, 2003

Symptoms of damage of forest trees influenced by abiotic stress
Uhlířová, H.; Novotný, R.; Matucha, Miroslav
2003 - Czech Keywords: forest ecosystem; coniferous species; pollutants Available at various institutes of the ASCR Symptoms of damage of forest trees influenced by abiotic stress

Uhlířová, H.; Novotný, R.; Matucha, Miroslav
Ústav experimentální botaniky, 2003

Vliv TCA na smrk ztepilý
Forczek, Sándor; Albrechtová, Jolana; Matucha, Miroslav; Uhlířová, H.
2003 - Czech Keywords: TCA Available at various institutes of the ASCR Vliv TCA na smrk ztepilý

Forczek, Sándor; Albrechtová, Jolana; Matucha, Miroslav; Uhlířová, H.
Ústav experimentální botaniky, 2003

Documentation of some botanical objects by table scanners
Babůrek, Ivan
2002 - Czech Table scanners may be used for documentation of biological, and especially flat botanical, objects in laboratory. In comparison with (digital) photography is picture from scanner mostly cheaper and quicker. In comparison with classical botany herbaria picture better catch the shape and colour of fresh biological object. Small 3D objects (seeds, fruits) may be also scanned without problems. Resolution 600 dpi is higher than that of human eye. Scanned picture may be used instead of using small magnifying glass. Stolní skenery mohou být využity pro dokumentaci biologických, a zvláště botanických, plochých objektů v laboratoři stejně dobře, jako (digitální) fotodokumentace. Lépe než herbářové položky zachycují tvar a barevnost, oproti fotografické dokumentaci jsou většinou rychlejší a levnější. Drobné trojrozměrné předměty (plody, semena) lze dokumentovat také. Obvyklé rozlišení 600 dpi je již vyšší než rozlišení lidského oka a může nahradit použití lupy při pozorování objektu. Keywords: botany; dokumentation; scanner; botanika Available at various institutes of the ASCR Documentation of some botanical objects by table scanners

Table scanners may be used for documentation of biological, and especially flat botanical, objects in laboratory. In comparison with (digital) photography is picture from scanner mostly cheaper and ...

Babůrek, Ivan
Ústav experimentální botaniky, 2002