No (89)
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Examination of the Spatial Structure of Pigs' Melanoma in Tissue Sections Based on Histology and Mass Spectrometry
Anýz, J.; Vysloužilová, L.; Horák, Vratislav; Štěpánková, O.; Vaculovič, T.; Adam, V.
2019 - English
We examine the spatial structure of the melanoma in tissue sections. The pigs melanoma was examined in 10 tissue samples obtained from animals of age between 4 and 22 weeks. The tissue sections were measured by light microscopy and Laser Ablation Ion Inductively Coupled Plasma Mass Spectrometry to obtain spatial metal (Cu, Zn) distribution. The exploratory analysis of the tissue sections indicates there is clearly a pattern in the spatial structure. Different projections of the spatial structure of the melanoma are obtained by the different measurement methods. The spectral clustering on the data was utilized to describe the structure in the data. According to the clustering results, there are distinct clusters of observations in the histological data. The spatial elemental distribution of the metals Cu and Zn cannot be clustered -the data form one compact cluster. The clustering of the histological images produces clusters which are related to the annotation of the biological samples in broader terms -the differences between fibrous and cancerous tissue.
Keywords:
MeLiM; melanoma
Available at various institutes of the ASCR
Examination of the Spatial Structure of Pigs' Melanoma in Tissue Sections Based on Histology and Mass Spectrometry
We examine the spatial structure of the melanoma in tissue sections. The pigs melanoma was examined in 10 tissue samples obtained from animals of age between 4 and 22 weeks. The tissue sections were ...
Bacteriophage 936 by qPCR quantification in dairy products.
Olša Fliegerová, Kateřina; Mrázek, Jakub; Štrosová, Lenka; Němečková, I.; Kopečný, Jan
2018 - Czech
Virulent bacteriophages are still representing significant threat in dairy production even with frequent disinfection and sterilization all the equipment in dairy plants. Therefore a reliable \nmethod of phage monitoring and quantification necessary part of phage prevention. Method is based on qPCR amplification and fluorescent detection of copies number of the phage main\nstructural protein and therefore is independent on the phage cultivation. It is quick, reliable a sensitive enough to estimate phage copy numbers in dairy starters and whey in any phase of dairy production. \n Virulentní bakteriofágy stále představují vážnou hrozbu pro průmyslové fermentace mléka, a to i při časté dezinfekci a sterilizaci všech zařízení v mlékárenských provozech. Proto je spolehlivá metoda monitorování přítomnosti bakteriofágů, ale zejména jejich kvantifikace, nutnou součástí boje proti virové infekci. Tato práce popisuje kvantifikaci laktokokového bakteriofága 936 pomocí polymerázové řetězové reakce (qPCR) a aplikaci tohoto postupu na vzorky provozních zákysů a syrovátek. Metoda je založena na amplifikaci a fluorescenční detekci nárůstu počtu kopií úseku genu pro hlavní strukturální protein fága 936 a je tudíž nezávislá na kultivaci bakteriofága. Metoda qPCR je rychlá, citlivá, umožňuje kvantifikaci bakteriofága 936 v malých objemech průmyslových vzorků a v kterékoliv fázi fermentačního procesu.\n
Keywords:
Bacteriophage 936; dairy products; lactobacillus
Available at various institutes of the ASCR
Bacteriophage 936 by qPCR quantification in dairy products.
Virulent bacteriophages are still representing significant threat in dairy production even with frequent disinfection and sterilization all the equipment in dairy plants. Therefore a reliable \nmethod ...
Sequencing of DNA variable fragment coding aspartyl-tRNA synthetase suitable for taxonomic classification and phylogenetic analysis of bacterial genera closely related to genus Lactobacillales \n
Killer, Jiří; Mrázek, Jakub
2018 - Czech
There was designed and tested a reliable marker for aspartyl-tRNA synthetase suitable for taxonomic classification and phylogenetic analysis of bacterial genera closely related to genus Lactobacillales. Method enables reliable a sensitive estimation in dairy starters and is independent on the phage cultivation. It is quick, reliable a sensitive estimation of dairy bacteria. It is possible to use it for basic research as well as for the effective analysis in dairy production plants. \n Byl připraven a ověřen marker (aspartyl-tRNA syntáza) pro klasifikaci a fylogenetické analýzy zástupců rodů náležejících do řádu Lactobacillales. Umožňuje rychlé a přesné druhové stanovení mléčných bakterií. Předkládanou metodiku lze využít v základním výzkumu při klasifikaci a fylogenetických analýzách vybraných rodů bakterií mléčného kvašení. Především je určen pro rychlé analýzy v provozech mléčného průmyslu. \n
Keywords:
Lactobacillales; PCR; marker
Available at various institutes of the ASCR
Sequencing of DNA variable fragment coding aspartyl-tRNA synthetase suitable for taxonomic classification and phylogenetic analysis of bacterial genera closely related to genus Lactobacillales \n
There was designed and tested a reliable marker for aspartyl-tRNA synthetase suitable for taxonomic classification and phylogenetic analysis of bacterial genera closely related to genus ...
Phenotypic Analyses of the HD Transgenic Minipig Model (A11609)
Ellederová, Zdeňka
2018 - English
The transgenic Huntington's disease minipigs (TgHD) express N‐terminal part of human mutated huntingtin (124Q) under the control of human huntingtin promoter. The founder animal, born in 2009, gave birth to four subsequent generations with an equal contribution of wild‐type (WT) and transgenic (TgHD) piglets in all litters. The model is being used for preclinical huntingtin lowering studies. Here we take different non-invasive and invasive approaches, some of which are unique for large animal models, to study the phenotype development comparing WT and TgHD siblings. We show gradual progression of the disease in these TgHD animals. Moreover, some biomarkers were identified. These markers could serve for monitoring of organism response to HD treatment to assess efficacy and safety in preclinical studies prior to human clinical trials.
Keywords:
minipig
Available at various institutes of the ASCR
Phenotypic Analyses of the HD Transgenic Minipig Model (A11609)
The transgenic Huntington's disease minipigs (TgHD) express N‐terminal part of human mutated huntingtin (124Q) under the control of human huntingtin promoter. The founder animal, born in 2009, gave ...
Establishing preclinical proof-of-concept of gene therapy for Huntington disease
Miniariková, J.; Juhás, Štefan; Caron, N.; Spronck, L.; Vallés, A.; De Haan, M.; Blits, B.; Ellederová, Zdeňka; van Deventer, S.; Petry, H.; Southwell, A.; Déglon, N.; Motlík, Jan; Konstantinová, P.; Evers, M.
2017 - English
Huntington disease (HD) is an autosomal dominant neurodegenerative disorder caused by a CAG trinucleotide repeat expansion in the HTT gene. The translated expanded polyglutamine repeat in the huntingtin protein is known to cause toxic gain-of-function, affecting numerous cellular processes. Our approach involves a new therapeutic modality by developing a single (one-time) treatment for HD based on a gene therapy lowering the expression of the toxic huntingtin using the RNA interference (RNAi) mechanism. Huntingtin lowering is achieved using gene transfer of a cassette encoding an engineered microRNA targeting human HTT, delivered via adeno-associated viral vector serotype 5 (AAV5-miHTT).
Keywords:
Huntington´s disease
Available at various institutes of the ASCR
Establishing preclinical proof-of-concept of gene therapy for Huntington disease
Huntington disease (HD) is an autosomal dominant neurodegenerative disorder caused by a CAG trinucleotide repeat expansion in the HTT gene. The translated expanded polyglutamine repeat in the ...
Oxidative stress in primary porcine fibroblasts expressing mutated huntingtin
Šmatlíková, Petra; Askeland, G.; Vaškovičová, Michaela; Klíma, Jiří; Motlík, Jan; Eide, L.; Ellederová, Zdeňka
2017 - English
Molecular events, such as protein aggregation, mitochondrial dysfunction, and transcriptional dysregulation have been linked to Huntington’s disease (HD) pathogenesis. Oxidative stress has been considered as one of the key players in disease progression. Though, it is still not clear whether oxidative stress causes HD, or if it is a consequence of other primary events.
Keywords:
huntingtin
Available at various institutes of the ASCR
Oxidative stress in primary porcine fibroblasts expressing mutated huntingtin
Molecular events, such as protein aggregation, mitochondrial dysfunction, and transcriptional dysregulation have been linked to Huntington’s disease (HD) pathogenesis. Oxidative stress has been ...
Role of planar cell polarity pathway in ameloblastoma with focus on frizzled 6
Putnová, Barbora; Putnová, Iveta; Hrubá, Eva; Dosedělová, Hana; Štembírek, Jan; Daněk, Z.; Buchtová, Marcela
2017 - English
Ameloblastoma is the most common epithelial odontogenic tumour in the oral cavity. Its progression is usually slow and the surface is covered by an oral mucosa with physiologic appearance. These tumours do not cause any pain to the patient and therefore it is difficult to diagnose them on time and the first signs are often missed by clinician leading to late diagnosis.
Keywords:
ameloblastoma
Available at various institutes of the ASCR
Role of planar cell polarity pathway in ameloblastoma with focus on frizzled 6
Ameloblastoma is the most common epithelial odontogenic tumour in the oral cavity. Its progression is usually slow and the surface is covered by an oral mucosa with physiologic appearance. These ...
Results of the realisation of the experiments using large experimental animals – application of cells/ stem cells using different vectors, diagnostics
Juhás, Štefan; Juhásová, Jana
2017 - English
A design of methods to test application of cells/ stem cells using different vectors, solution of the application including diagnostics and connected agenda.
Keywords:
large animal models; stem cell therapy; spinal stem cells
Available at various institutes of the ASCR
Results of the realisation of the experiments using large experimental animals – application of cells/ stem cells using different vectors, diagnostics
A design of methods to test application of cells/ stem cells using different vectors, solution of the application including diagnostics and connected agenda.
In vivo IBD using minipig course results
Juhás, Štefan; Juhásová, Jana
2017 - Czech
Innovative approaches testing of Inflammatory Bowel Disease on minipigs. Testování inovativních postupů léčby IBD na miniaturních prasatech.
Keywords:
minipig; IBD; operational equipment
Available at various institutes of the ASCR
In vivo IBD using minipig course results
Innovative approaches testing of Inflammatory Bowel Disease on minipigs.
Results of the realisation of the experiments using the experimental animals
Juhás, Štefan; Juhásová, Jana
2017 - Czech
A design of methods to test a functionality of inovative replacements, solution of the replacement incl. implantation and connected agenda. Návrh způsobu ověření funkčnosti inovativních náhrad, řešení tohoto návrhu včetně implantací a souvisejících aktivit.
Keywords:
implants; experimental animals
Available at various institutes of the ASCR
Results of the realisation of the experiments using the experimental animals
A design of methods to test a functionality of inovative replacements, solution of the replacement incl. implantation and connected agenda.
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