Significance of the acrosome reaction and sperm agglutinating antibodies in human semen. acrosome RE
Pavlásek, J.; Pěknicová, Jana; Ulčová-Galová, Z.; Nováková, P.; Mičanová, Z.; Rokyta, Z.
2003 - English We commpared results of the acrosome reaction and sperm agglutinating antibodies in human semen samples. Keywords: acrosome reaction; monoclonal antibody Available at various institutes of the ASCR Significance of the acrosome reaction and sperm agglutinating antibodies in human semen. acrosome RE

We commpared results of the acrosome reaction and sperm agglutinating antibodies in human semen samples.

Pavlásek, J.; Pěknicová, Jana; Ulčová-Galová, Z.; Nováková, P.; Mičanová, Z.; Rokyta, Z.
Ústav molekulární genetiky, 2003

Characterization of peptide proteinase inhibitors isolated from boar seminal plasma
Jelínková, Petra; Tichá, M.; Jonáková, Věra
2003 - English Seminal plasma of different mammalian species contains various types of proteins. Proteinase inhibitors are important components of seminal plasma and were found in all tested species. In the present communication we have shown that serine proteinase inhibitors participate (under physiological conditions) in the formation of associated complexes with other proteins from boar seminal plasma. Keywords: boar seminal plasma proteins; proteinase inhibitors Available at various institutes of the ASCR Characterization of peptide proteinase inhibitors isolated from boar seminal plasma

Seminal plasma of different mammalian species contains various types of proteins. Proteinase inhibitors are important components of seminal plasma and were found in all tested species. In the present ...

Jelínková, Petra; Tichá, M.; Jonáková, Věra
Ústav molekulární genetiky, 2003

Protein Characterization of Boar Epididymal Fluid
Maňásková, Pavla; Tichá, M.; Jonáková, Věra
2003 - English Keywords: epididymal maturation, proteins, fertilization Available at various institutes of the ASCR Protein Characterization of Boar Epididymal Fluid

Maňásková, Pavla; Tichá, M.; Jonáková, Věra
Ústav molekulární genetiky, 2003

Role of sperm surface proteins in gamete binding
Jonáková, Věra; Tichá, M.
2003 - English Mammalian fertilization includes highly regulated biochemical interactions between complementary molecules located on the surface of both gametes. Sperm surface proteins play various roles in the fertilization process. Keywords: boar seminal plasma proteins, gamete binding, fertilization Available at various institutes of the ASCR Role of sperm surface proteins in gamete binding

Mammalian fertilization includes highly regulated biochemical interactions between complementary molecules located on the surface of both gametes. Sperm surface proteins play various roles in the ...

Jonáková, Věra; Tichá, M.
Ústav molekulární genetiky, 2003

Study of sperm proteins in modelspecies of chondrostean and teleostean fish
Koubek, Pavel; Elzeinová, Fatima; Linhart, O.; Pěknicová, Jana
2003 - English Specific in the study of fish reproduction is outer fertilization. spermatozoa are usually expelled into host medium of the outer encironment. The cells have to cope with an extremely different encironment and their protection against the outer environment is much weaker than that of oocytes. The way of fertilization also differs from mammalian fertilization. Keywords: cyprinus; carpio; acipenser Available at various institutes of the ASCR Study of sperm proteins in modelspecies of chondrostean and teleostean fish

Specific in the study of fish reproduction is outer fertilization. spermatozoa are usually expelled into host medium of the outer encironment. The cells have to cope with an extremely different ...

Koubek, Pavel; Elzeinová, Fatima; Linhart, O.; Pěknicová, Jana
Ústav molekulární genetiky, 2003

Dynamic and role of cytosekeletal proteins in mammalian sperm cells
Paleček, J.; Dvořáková, K.; Pěknicová, Jana; Kubátová, Alena; Moore, H. D. M.
2003 - English The cytoskeleton of sperm head consists of resistant structural proteins of the nucleus and of perinuclear theca, which are largely rosponsible for the shaping of the nucleus. three mahjor cyteskeletal proteins, actin, tzubnulin and spectrin, are present in the head of mammalian spermatohzoa. Maoreober, the mictotubular cytoskeleton also contains other dynamic proteins, which have a morphological function within the cell and which are responsible for cell signalling processes. Keywords: cytoskeleton; spermatozoa Available at various institutes of the ASCR Dynamic and role of cytosekeletal proteins in mammalian sperm cells

The cytoskeleton of sperm head consists of resistant structural proteins of the nucleus and of perinuclear theca, which are largely rosponsible for the shaping of the nucleus. three mahjor ...

Paleček, J.; Dvořáková, K.; Pěknicová, Jana; Kubátová, Alena; Moore, H. D. M.
Ústav molekulární genetiky, 2003

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Teplá, O.; Pěknicová, Jana; Mrázek, M.; Kočí, K.; Chládek, David; Míka, M.; Badalová, J.; Pecka, T.; Fulka, F.
2003 - English Iscare IVF a.s. had a long record of successful azoospermic treatment since 1997, when the first pregnancies by MESA a TESE were published in the Czech republic. A series of diferent implanted laboratory techniques supported good results from last year. The medical problem is solved during the other field of application-histlogical findings and immunofluorescent methods. Keywords: MESA; azoospermia; TESE Available at various institutes of the ASCR noTitle

Iscare IVF a.s. had a long record of successful azoospermic treatment since 1997, when the first pregnancies by MESA a TESE were published in the Czech republic. A series of diferent implanted ...

Teplá, O.; Pěknicová, Jana; Mrázek, M.; Kočí, K.; Chládek, David; Míka, M.; Badalová, J.; Pecka, T.; Fulka, F.
Ústav molekulární genetiky, 2003

Regulation and localization of sperm akaps during capacitation, activation, induced acrosomal reaction and binding to the oocyte
Kyselová, Vendula; Pěknicová, Jana
2003 - English Recent evidence suggests that the cAMP-dependent protein kinase A (PKA) is localized to specific subcellulatr compatrments trought association with A Kinase Anchoring Proteins (AKAPs). The AKAPs now represent a functionally related family of regulatory proteins containing a conserved PKA binding domain and a unique targeting sequence that direct the PKA-AKAP complex to subcellular structures. Keywords: akap; acrosome Available at various institutes of the ASCR Regulation and localization of sperm akaps during capacitation, activation, induced acrosomal reaction and binding to the oocyte

Recent evidence suggests that the cAMP-dependent protein kinase A (PKA) is localized to specific subcellulatr compatrments trought association with A Kinase Anchoring Proteins (AKAPs). The AKAPs now ...

Kyselová, Vendula; Pěknicová, Jana
Ústav molekulární genetiky, 2003

Validation of macromolecular crystal structures
Řezáčová, Pavlína
2003 - Czech X-ray crystallography is the main technique of macromolecular structure determination. Typical problems of protein crystallography diffraction data measured at limited resolution and inaccurate phase informacion adversely affect the quality of electron density maps and consequently the quality of the final model. Currently, there are many methods of protein quality validation. These can be subdivided into two main groups The first group assesses the model quality by evaluating the agreement of the model with the diffraction data. The second group of structure validation methods is based on comparison of the macromolecular model geometrical parameters with standards values derived from the crystal structures of small molecules or high-resolution macromolecular structures. This work is focused on introduction to widely used macromolecular structure-validation software. The main global quality criteria and reliable indicators for detecting local errors are summarized. Rentgenová krystalografie je hlavní technikou získání detailní informace o atomové struktuře makromolekul. Typické problémy makromolekulární krystalografie: difrakční data získaná na omezeném rozlišení a nepřesná fázová informace, nevyhnutelně ovlivňují kvalitu map elektronových hustot a následně se odrazí i v kvalitě konečného modelu.Dnes existuje mnoho metod pro ověření kvality modelu, které lze rozdělit do dvou základních skupin. První skupina hodnotí kvalitu modelu podle jeho shody s experimentálními daty. Druhá skupina ověřovacích metod hodnotí geometrické parametry modelu srovnáním se standardními hodnotami odvozenými z krystalových struktur malých molekul nebo struktur makromolekul určených na vysokém rozlišení. Tato práce je věnována představení běžně používaných počítačových programů využívajících obou výše zmíněných metod hodnocení kvality a ověřování struktur makromolekul. Jsou zde shrnuty ukazatele a měřítka kvality užívaná při hodnocení krystalových struktur makromolekul. Keywords: protein crystallography; validation; stereochemistry Available at various institutes of the ASCR Validation of macromolecular crystal structures

X-ray crystallography is the main technique of macromolecular structure determination. Typical problems of protein crystallography diffraction data measured at limited resolution and inaccurate phase ...

Řezáčová, Pavlína
Ústav molekulární genetiky, 2003

Processing of Diffraction Data Obtained by Oscillation Method on Crystals of Biological Macromolecules
Brynda, Jiří
2003 - Czech The components of monochromatic diffractometer suitable for data collection on crystals of biological macromolecules are described, as well as basics of rotation method. The optimal strategy for collecting X-ray diffraction data from macromolecular crystals is discussed. The geometric factors influencing the completeness of data are arising from the symmetry of the reciprocal lattice and from the experimental setup; they affect quantitatively the completeness of the measured set of reflections. Příspěvek popisuje jednotlivé komponenty monochromatického difraktometru vhodného pro měření krystalů biologických makromolekul a také základní principy rotační metody. Optimální strategie pro měření difrakčních dat na krystalech makromolekul je diskutována. Geometrické faktory ovlivňující kompletnost dat jsou symetrie reciproké mřížky a geometrické uspořádání jednotlivých komponent monochromatického difraktometru, které ovlivňují kvantitativně kompletnost měřených dat. Keywords: X-ray data collection, rotation method, X-ray diffractometer Available at various institutes of the ASCR Processing of Diffraction Data Obtained by Oscillation Method on Crystals of Biological Macromolecules

The components of monochromatic diffractometer suitable for data collection on crystals of biological macromolecules are described, as well as basics of rotation method. The optimal strategy for ...

Brynda, Jiří
Ústav molekulární genetiky, 2003