Yes (1182)
Used filters
(3)
Number of found documents:
1182
Published from
to
Thin Nanostructured Film with Bioapplication Potential
KUMAR, Sanjay
2024 - English
The Ph.D. thesis aims to investigate, develop, and fabricate advanced nanostructured surfaces for (bio)sensors and detection applications. The primary outcome of the research is the development of a functional sensor transducer for the detection of specific anti-DbpA antibodies, typically produced by a living organism in response to Lyme disease infection, as well as for detecting live Borreliosis pathogens. The developed sensor is based on AgNPs/C:H:N:O plasma polymer nanocomposites. Silver (Ag) nanoparticles, generated by a gas aggregation source, are incorporated into a C:H:N:O plasma polymer matrix, which is deposited by magnetron sputtering of nylon 6.6. The surface of the AgNPs/C:H:N:O nanocomposite is functionalized with amino-rich -NH2 grafts and specific antibodies to achieve high specificity in detection. Anchored Ag nanoparticles propagate localized surface plasmon resonance (LSPR), optically responding to changes caused by immobilized targets near the nanoparticles. The sensitivity of the sensor transducer is enhanced by incorporating Cu nanoparticles, which contribute to Cu-Ag coupling modes responsible for the modulation of the LSPR bands. The achieved results pave the way for rapid, low-cost, sensitive platforms for detecting not only the antibodies/pathogens studied here but also potentially other targets. The Ph.D. thesis aims to investigate, develop, and fabricate advanced nanostructured surfaces for (bio)sensors and detection applications. The primary outcome of the research is the development of a functional sensor transducer for the detection of specific anti-DbpA antibodies, typically produced by a living organism in response to Lyme disease infection, as well as for detecting live Borreliosis pathogens. The developed sensor is based on AgNPs/C:H:N:O plasma polymer nanocomposites. Silver (Ag) nanoparticles, generated by a gas aggregation source, are incorporated into a C:H:N:O plasma polymer matrix, which is deposited by magnetron sputtering of nylon 6.6. The surface of the AgNPs/C:H:N:O nanocomposite is functionalized with amino-rich -NH2 grafts and specific antibodies to achieve high specificity in detection. Anchored Ag nanoparticles propagate localized surface plasmon resonance (LSPR), optically responding to changes caused by immobilized targets near the nanoparticles. The sensitivity of the sensor transducer is enhanced by incorporating Cu nanoparticles, which contribute to Cu-Ag coupling modes responsible for the modulation of the LSPR bands. The achieved results pave the way for rapid, low-cost, sensitive platforms for detecting not only the antibodies/pathogens studied here but also potentially other targets.
Keywords:
plasma polymer; plasma polymerization; C:H:N:O thin film; nylon; nanoparticles (Ag; Cu); nanostructures; nanocomposite; surface functionalization; magnetron sputtering; annealing; adhesion; immobilization; sensors; antibacterial; Lyme disease; Borrelia; plasmonic absorption; localized surface plasmon resonance (LSPR); plasma-assisted deposition; enhanced detection
Available in the Digital Repository of University of South Bohemia.
Thin Nanostructured Film with Bioapplication Potential
The Ph.D. thesis aims to investigate, develop, and fabricate advanced nanostructured surfaces for (bio)sensors and detection applications. The primary outcome of the research is the development of a ...
Population genomics of parasite adaptation: New insights into diversification and speciation of parasites
NAZARIZADEH DEHKORDI, Masoud
2024 - English
This thesis investigates the ecological and genetic dynamics of parasitic species, primarily focusing on Ligula intestinalis tapeworms and Cimex bedbugs, aiming to enhance our understanding of their evolutionary biology, genetics, and adaptation mechanisms. In Chapter I, it elucidates the prevalence and population genetic structure of L. intestinalis plerocercoids in various cyprinoid fish species across Czech water bodies, focusing on how different hosts and environments influence genetic diversity and distribution. Chapter II explores the historical dispersal and host-switching events that have shaped the evolutionary history of the L. intestinalis species complex on a global scale. This includes identifying major evolutionary lineages, their host specificity, and the influence of biogeographic and demographic events on lineage diversity. Chapter III provides a highly contiguous genome assembly for L. intestinalis and examines RNA transcription patterns to understand the regulation of biological functions during its life cycle, establishing a comprehensive reference transcriptome for both plerocercoid and adult stages. Chapter IV explores the mechanisms underlying possible ecological speciation in L. intestinalis, particularly under a sympatric setting, to determine whether host specificity influences population structure in the absence of geographical separation. Chapter V shifts focus to the establishment of the tropical bedbug, Cimex hemipterus, in Central Europe, using genetic analyses to reveal limited mtDNA variability and the presence of pyrethroid resistance mutations. Finally, Chapter VI investigates genetic and transcriptomic divergence in C. lectularius populations associated with human and bat hosts, exploring how host switching influences genetic diversity and adaptation. This genomic and transcriptomic approach provides valuable insights into the evolutionary dynamics and adaptive strategies of parasitic species.
Keywords:
Population genomics; Phylogeography; Historical biogeography; Ligula intestinalis; bedbugs
Available in the Digital Repository of University of South Bohemia.
Population genomics of parasite adaptation: New insights into diversification and speciation of parasites
This thesis investigates the ecological and genetic dynamics of parasitic species, primarily focusing on Ligula intestinalis tapeworms and Cimex bedbugs, aiming to enhance our understanding of their ...
Tuning carotenoid photophysics by changes in structure and local environment
ÖZCAN, Emrah
2024 - English
This thesis investigates the complex dynamics of excited states in carotenoids, focusing on the origins and properties of certain dark states and how they are affected by external disturbances such as metal interactions, pH changes, applied voltage, and chemical modifications. Paper I demonstrates the creation of stable astaxanthin-metal complexes and their altered excited-state dynamics, revealing shortened lifetimes and enhanced ICT bands due to metal-ion interactions. Paper II explores the effect of external voltage on 8-apo--carotenal, showing that applied voltage can control the ICT state amplitude and extend its lifetime. Paper III examines pH-dependent effects on crocin, finding significant pH-induced shifts and stabilization at basic pH levels. Paper IV presents the synthesis and analysis of bis-phenylhydrazone astaxanthin, highlighting its modified excited-state dynamics and the potential for organic modification to study new effects. These findings enhance our understanding of carotenoid excited states and suggest new research approaches. This thesis investigates the complex dynamics of excited states in carotenoids, focusing on the origins and properties of certain dark states and how they are affected by external disturbances such as metal interactions, pH changes, applied voltage, and chemical modifications. Paper I demonstrates the creation of stable astaxanthin-metal complexes and their altered excited-state dynamics, revealing shortened lifetimes and enhanced ICT bands due to metal-ion interactions. Paper II explores the effect of external voltage on 8-apo--carotenal, showing that applied voltage can control the ICT state amplitude and extend its lifetime. Paper III examines pH-dependent effects on crocin, finding significant pH-induced shifts and stabilization at basic pH levels. Paper IV presents the synthesis and analysis of bis-phenylhydrazone astaxanthin, highlighting its modified excited-state dynamics and the potential for organic modification to study new effects. These findings enhance our understanding of carotenoid excited states and suggest new research approaches.
Keywords:
Carotenoids; Metal complexes; Ultrafast spectroscopy; Charge transfer state; Cyclic voltammetry; 8'-apo--carotenal; Spectroelectrochemistry; Crocin; pH-dependence.
Available in the Digital Repository of University of South Bohemia.
Tuning carotenoid photophysics by changes in structure and local environment
This thesis investigates the complex dynamics of excited states in carotenoids, focusing on the origins and properties of certain dark states and how they are affected by external disturbances such as ...
Consumptive and non-consumptive effect of predators and food quality on arthropod herbivores
KOLLROSS, Jan
2024 - English
Top-down and bottom-up controls are crucial mechanisms that shape ecosystems and biodiversity. In this thesis, I investigate both bottom-up and top-down forces, their interplay and specific effects on arthropod communities, herbivores, and their behaviour across vertical forest gradients. The thesis consists of four chapters. The first chapter explores the top-down regulation of arthropods and the resulting herbivore damage across a vertical forest gradient in a Japanese temperate forest, focusing on the effect of three groups of insectivorous predators. In my second chapter, I attempt to compare the relative impact of bottom-up and top-down control. I studied their relative effects on arthropods and herbivore damage by manipulating nutrient availability and access of predators to trees in a temperate forest in the Czech Republic. In my third chapter, I focused on one of the most important insect orders, the Coleoptera. I investigated the Coleoptera communities and the influence of predators on them along the vertical forest gradient in two different temperate sites: in Japan and Germany. Finally, I examined the nonlethal effect of predators on herbivore prey, testing the effect of bird presence on locust behavior and their stress hormone levels. Overall, my thesis examines the complexity of tri-trophic interactions between plants, herbivorous arthropods, and their insectivorous predators. It highlights the importance of nutrient availability, and the access of insectivorous predators to plants, for the functioning and result of multi-trophic interactions. It also discusses lethal as well as nonlethal effects separately. Top-down and bottom-up controls are crucial mechanisms that shape ecosystems and biodiversity. In this thesis, I investigate both bottom-up and top-down forces, their interplay and specific effects on arthropod communities, herbivores, and their behaviour across vertical forest gradients. The thesis consists of four chapters. The first chapter explores the top-down regulation of arthropods and the resulting herbivore damage across a vertical forest gradient in a Japanese temperate forest, focusing on the effect of three groups of insectivorous predators. In my second chapter, I attempt to compare the relative impact of bottom-up and top-down control. I studied their relative effects on arthropods and herbivore damage by manipulating nutrient availability and access of predators to trees in a temperate forest in the Czech Republic. In my third chapter, I focused on one of the most important insect orders, the Coleoptera. I investigated the Coleoptera communities and the influence of predators on them along the vertical forest gradient in two different temperate sites: in Japan and Germany. Finally, I examined the nonlethal effect of predators on herbivore prey, testing the effect of bird presence on locust behavior and their stress hormone levels. Overall, my thesis examines the complexity of tri-trophic interactions between plants, herbivorous arthropods, and their insectivorous predators. It highlights the importance of nutrient availability, and the access of insectivorous predators to plants, for the functioning and result of multi-trophic interactions. It also discusses lethal as well as nonlethal effects separately.
Keywords:
Bottom-up control; top-down control; arthropod communities; herbivory; vertical forest gradient; nutrient availability; predator presence; trophic cascades; ecosystem dynamics; nonlethal predator effects; Coleoptera; temperate forests
Available in the Digital Repository of University of South Bohemia.
Consumptive and non-consumptive effect of predators and food quality on arthropod herbivores
Top-down and bottom-up controls are crucial mechanisms that shape ecosystems and biodiversity. In this thesis, I investigate both bottom-up and top-down forces, their interplay and specific effects on ...
Impact of water deficit stress on leaf quality, predator and herbivore communities in Ficuses
XIAO, Xue
2024 - English
This thesis addresses the mechanism by which drought affects tropical plant-insect interactions via leaf traits, focusing on Ficus species. I discovered that both drought intensity and duration indirectly influenced the leaf palatability of Ficus through changes in leaf mechanical traits such as leaf water content and specific leaf area. Furthermore, my research revealed that drought increased leaf herbivory by altering leaf latex outflow, without affecting insect communities. Additionally, I observed that drought enhanced Ficus indirect defense through changes in herbivore-induced plant volatiles. My findings suggest that the impact of drought on Ficus-insect interactions may vary between greenhouse and tropical rainforest environments and may be species-specific. This work underscores the importance and urgency of researching tropical plant-insect interactions under climate change.
Keywords:
drought; herbivores; leaf mechanical traits; leaf secondary metabolites; leaf volatile; predators; tropical ecosystem
Available in the Digital Repository of University of South Bohemia.
Impact of water deficit stress on leaf quality, predator and herbivore communities in Ficuses
This thesis addresses the mechanism by which drought affects tropical plant-insect interactions via leaf traits, focusing on Ficus species. I discovered that both drought intensity and duration ...
Patrnosti v polinačních systémech podél geografických gradientů
JACKWERTH, Karolína
2024 - English
The general aim of the thesis is to shed light on invertebrate herbivory and pollination in relation to the changing environment. Several experiments were conducted on meadow communities in the temperate region, and we combined manipulative and experimental approaches during data collection. The first chapter of the thesis investigated the change of invertebrate herbivory across one season affected by phylogeny and plant functional traits. The second chapter emphasized the impact of range-shifting plants, driven by increasing temperature as a proxy for global warming, on herbivory damage. Similarly, the third chapter focused on plant-pollinator turnover on range-shifting plants, driven by increasing temperature as a proxy for global warming. The last fourth chapter examined the effect of increasing temperature as a proxy for global warming on pollen viability of widespread forb.
Keywords:
invertebrate herbivory; insect pollination; range-shifting plants; elevation
Available in the Digital Repository of University of South Bohemia.
Patrnosti v polinačních systémech podél geografických gradientů
The general aim of the thesis is to shed light on invertebrate herbivory and pollination in relation to the changing environment. Several experiments were conducted on meadow communities in the ...
Enhancement of fish communities, improvement of sampling and stock assessment in lakes and reservoirs.
RIBEIRO DE MORAES, Karlos
2024 - English
This Ph.D. thesis focuses on fish habitats, how we can improve their natural complexity, and how we can improve techniques to survey these habitats. With increasing human activities affecting freshwater ecosystems due to human population growth and industrial expansion, it is crucial to study environmental changes, their impacts, and ways to mitigate damage. As many biotic and abiotic factors can influence the health and diversity of the community, for our study, we focused on habitat definition and complexity, and the effect of fishery management. Paper (I) focuses on the transition between littoral (shallow areas) and pelagic (open water) habitats and their impact on fish communities. The littoral zone, typically the most intricate segment of a water body, serves as the primary habitat for a significant portion of the fish community and species diversity. However, its spatial extent is very limited. Littoral delimitation is important for obtaining a true picture of the fish community composition and for its sustainable management decisions. Paper (II) investigated the impact of protected areas on fish populations in the Lipno reservoir in the Czech Republic, specifically focusing on the abundance, biomass, standard length, and diversity indices. Protected areas have legislation that reduces or stops certain anthropological impacts to help recover the ecosystem. These areas are normally linked to spawning areas, feeding grounds, or rare species and are vital for the restoration and proliferation of specific environments. In our case, we revealed more and larger predatory fish in protected and low anthropological impact areas. Paper (III) tests the introduction of artificial habitats, which are man-made structures or environments created to provide additional habitats and support for various organisms. Despite efforts to restore habitats to their natural states, there are instances where complete restoration is challenging. In particular, water bodies are subjected to significant anthropogenic alterations, such as reservoirs. In such instances, the introduction of artificial habitats has emerged as a solution to rapidly enhance the complexity of these environments. In Lipno reservoir, artificial floating islands attracted young-of-the-year of common species.
Keywords:
Fish management; Anthropogenic impact; Spatial distribution; Habitat use; Habitat restoration; Stock enhancement; Protected areas; Artificial Habitat
Available in the Digital Repository of University of South Bohemia.
Enhancement of fish communities, improvement of sampling and stock assessment in lakes and reservoirs.
This Ph.D. thesis focuses on fish habitats, how we can improve their natural complexity, and how we can improve techniques to survey these habitats. With increasing human activities affecting ...
The role of ecological gradients and host-parasitoid interaction in tropical insect communities
FINNIE, Sam Cameron
2024 - English
The thesis explores the various biotic and abiotic factors that drive change in insect communities with particular emphasis on host-parasitoid interactions. These factors range from ecological gradients to defensive traits and host specialisation. The aim of the first chapter was to determine how the assemblage of an ecologically and anthropocentrically significant group of insects changed in response to altitude. This study highlighted the elevational limit of fruit flies and discovered that a new and promising attractant lure used in agriculture was particularly useful at higher altitudes, potentially opening new avenues for pest management in mountainous regions. The aim of the second chapter was to ascertain the physiological and phylogenetic traits of galling insects that alter the diversity and host specificity of their parasitioids. This research not only uncovered a remarkably specialist community of gall parasitoids, and found significant physiological drivers of parasitoid assemblage, it also broached the possibility of a latitudinal gradient in gall parasitoid host specificity, that warrants further exploration due to its potential implications for biodiversity patterns. The final chapter, aimed to uncover patterns in the vertical stratification of a caterpillar-parasitoid community, and whether any potential patterns and interactions are driven by the defensive traits of the caterpillar hosts. This study provided further evidence that the defensive traits of caterpillars influence their vulnerability to parasitism, and discovered distinct patterns in the vertical stratification of caterpillar communities. Overall, this holistic research expands upon the existing literature on tropical insect ecology and advances our understanding on the complex interactions between insects and their environment. The thesis explores the various biotic and abiotic factors that drive change in insect communities with particular emphasis on host-parasitoid interactions. These factors range from ecological gradients to defensive traits and host specialisation. The aim of the first chapter was to determine how the assemblage of an ecologically and anthropocentrically significant group of insects changed in response to altitude. This study highlighted the elevational limit of fruit flies and discovered that a new and promising attractant lure used in agriculture was particularly useful at higher altitudes, potentially opening new avenues for pest management in mountainous regions. The aim of the second chapter was to ascertain the physiological and phylogenetic traits of galling insects that alter the diversity and host specificity of their parasitioids. This research not only uncovered a remarkably specialist community of gall parasitoids, and found significant physiological drivers of parasitoid assemblage, it also broached the possibility of a latitudinal gradient in gall parasitoid host specificity, that warrants further exploration due to its potential implications for biodiversity patterns. The final chapter, aimed to uncover patterns in the vertical stratification of a caterpillar-parasitoid community, and whether any potential patterns and interactions are driven by the defensive traits of the caterpillar hosts. This study provided further evidence that the defensive traits of caterpillars influence their vulnerability to parasitism, and discovered distinct patterns in the vertical stratification of caterpillar communities. Overall, this holistic research expands upon the existing literature on tropical insect ecology and advances our understanding on the complex interactions between insects and their environment.
Keywords:
Vertical stratification; elevational gradient; defensive traits; caterpillars; galls; tephritidea; parasitism rates; parasitoid richness
Available in the Digital Repository of University of South Bohemia.
The role of ecological gradients and host-parasitoid interaction in tropical insect communities
The thesis explores the various biotic and abiotic factors that drive change in insect communities with particular emphasis on host-parasitoid interactions. These factors range from ecological ...
Reverse Janzen-Connell effects in New Guinean ant-plants
ZAHRA, Shafia
2024 - English
In this thesis, I investigate the presence of reverse Janzen-Connell effects in ant-plant communities in Papua New Guinea. Janzen-Connell effects help to explain species diversity maintenance by focusing on natural enemies' role in controlling plant distributions. It is postulated that spillover of enemies near adult plants reduces seedling survival, promoting coexistence and diversity. Conversely, reverse Janzen-Connell effects occur when mutualists enhance survival near conspecific adults, potentially creating larger recruitment zones leading in the extreme to species monodominance. I use a combination of observation and ant exclusion experiments, as well as spatial analysis to explore reverse Janzen-Connell effects. Ant-plant interactions, particularly myrmecophytic ones, show moderate species specificity, a key assumption of classic Janzen-Connell effects. Mutualistic ants enhance plant viability by eliminating herbivores and pathogens, reducing damage, and optimizing photosynthesis. I also explore the specificity of myrmecophytic systems compared to other ant-plant interactions across environments using a data collation approach. I speculate that reverse Janzen-Connell effects can be observed in ant-plant mutualisms, ectomycorrhizal fungi, and plants. I also found through a global meta-analysis that myrmecophytic networks are the most specialized, with specialisation being driven by network type rather than environmental factors. Thus, myrmecophytes are a suitable system to explore the presence of reverse Janzen-Connell effects. Spatial analysis showed the presence of reverse Janzen-Connell effects in plants in seedlings of Meliaceae family and less strongly in adults. In the ant exclusion experiments, conspecific saplings exhibited higher survival and growth, but this was not related to the treatment itself meaning the plants are potentially benefiting from root fungi from adult conspecifics. Saplings with ant patrolling had better survival, although caution is needed due to small sample size. Lastly, I found that ant inhabitation positively influenced growth in older ant-plants, but this was not linked to local ant-plant density, and leaf loss from herbivory was lower when saplings were near adult conspecifics, possibly due to protective volatiles. Taken together this suggests some weak evidence for reverse JanzenConnell effects in the lowland rainforest of Papua New Guinea.
Keywords:
Ant-plants interaction; Papua New Guinea; Rypasora amplifolia; network specialization; Janzen-Connell effect; Reverse Janzen Connell effect; meta-analysis; Myrmecophytic.
Available in the Digital Repository of University of South Bohemia.
Reverse Janzen-Connell effects in New Guinean ant-plants
In this thesis, I investigate the presence of reverse Janzen-Connell effects in ant-plant communities in Papua New Guinea. Janzen-Connell effects help to explain species diversity maintenance by ...
Characterization of proteins, soluble gums and phenolic compounds isolated from flaxseed (Linum usitatissimum L.)
JAROŠOVÁ, Markéta
2024 - Czech
Flax (Linum usitatissimum L.) is one of the oldest domesticated crops. Its cultivation for fibre, oil or medicinal purposes was already widespread in ancient times. Nowadays, the growing of flax for the production of high-quality flaxseed oil prevails compared to fibres. However, using flaxseed as a functional food in the food industry and dietary applications has become more popular recently. Flaxseed contains bioactive components, including polyunsaturated fatty acids representing the major proportion of flaxseed oil, as well as functional proteins and peptides, lignans and dietary fibre in the forms of soluble mucilaginous polysaccharides and insoluble lignocellulose. This dissertation presents the results focused on characterisation of three significant constituents of flaxseed - proteins, polysaccharides and lignans. These groups of compounds exhibit interesting functional properties and biological activities that may be useful in a wide range of food or medical applications. This work highlights the importance and potential use of flaxseed oil cake, which is an important source of these components. In addition to the results, this thesis also provides a comprehensive literature review of previous and current research on flaxseed proteins, mucilages and lignans. The results of this work can be primarily applied to breeding new oilseed flax cultivars or in the food and processing industries. However, due to the important nutritional aspects and biological activities of the mentioned components, the obtained findings can also be used in dietary applications and medicine. In general, this work aims to expands the spectrum of knowledge on the subject and to open up the scope for further research. Two review articles represent the first part of the results. The first publication focused on the hydrocolloids of flaxseed in the form of flaxseed mucilage representing 4-15% of the flaxseed content and protein representing 20% of its weight. In this manuscript, the structure, extraction methods, functional and biological properties and possible applications of both types of hydrocolloids for the fortification of various foods or other food applications are described in detail. This manuscript is followed by a short review article summarising current trends in food and dietary applications of flaxseed mucilage. The first publication describes more likely the traditional use of flaxseed mucilage for food fortification to enhance the technological, nutritional or sensory quality of foods or to provide health benefits. The second review paper focuses exclusively on the potential use of flaxseed mucilage in recent and innovative food industry and nutrition areas. In modern applications, flaxseed mucilage can be applied, for instance for preparing films, for food protection, as a structural component in oleogels, cryogels or aerogels, and it may modify the functional properties of proteins within composites. From the point of nutrition, flaxseed mucilage can serve as an encapsulating agent for probiotics or biologically active compounds. Flaxseed mucilage has the characteristics of a prebiotic and may increase the proportion of beneficial probiotic microorganisms. As a result, both review articles highlight the significant potential of flaxseed proteins and flaxseed mucilage for various recent and future applications. The second part of this thesis represents a first-authorship original manuscript focused on researching the influence of variety and growing conditions on yield parameters, chemical composition, lignan content and antioxidant potential of flaxseeds. Len setý (Linum usitatissimum L.) se řadí mezi nejstarší domestikované plodiny. Jeho pěstování za účelem získávání vláken, oleje či pro lékařské účely bylo rozšířeno již v období starověku. Kromě, dnes již minoritní, produkce vláken využívaných v textilním průmyslu, a současně převažující pěstování pro produkci vysoce kvalitního oleje, se semena lnu v současné době uplatňují, jako funkční potravina spojená s širokými možnostmi aplikací v různých oblastech potravinářství a výživy. Lněná semena obsahují biologicky aktivní komponenty v podobě polynenasycených mastných kyselin tvořící hlavní podíl lněného oleje, dále pak vysoce kvalitní bílkoviny a peptidy, lignany a dietární vlákninu ve formě rozpustných polysacharidů tvořící sliz a nerozpustné lignocelulosy. Tato disertační práce předkládá výsledky zaměřené na charakterizaci tří významných komponentů lněného semene ? bílkovin, polysacharidů a lignanů. Tyto skupiny látek vykazují zajímavé funkční vlastnosti a biologické aktivity, jež mohou být využitelné v širokém spektru potravinářských aplikací nebo lékařství. Práce akcentuje význam a možnosti využití výlisků lněných semen, jež jsou významným zdrojem uvedených komponent. Kromě samotných výsledků je součástí této disertační práce také komplexní literární přehled o dřívějších a současných výzkumných pracích zaměřených na lněné bílkoviny, slizy a lignany. Poznatky získané v rámci této práce mohou být uplatnitelné zejména při novošlechtění olejného lnu, v potravinářském a zpracovatelském průmyslu. Nicméně, vzhledem k významným nutričním aspektům a biologickým aktivitám výše zmíněných složek lze získané poznatky využít rovněž v oblastech samotné výživy a lékařství. Obecně si tato práce klade za cíl rozšíření spektra znalostí dané problematiky a otevřít prostor pro další výzkum. První část výsledků této disertační práce představují dvě komplexní přehledové publikace. První publikace byla zaměřená na hydrokoloidy lněného semene v podobě lněného slizu představující 4-15 % obsahu lněného semene a bílkoviny tvořící 11-31 % jeho hmotnosti. V tomto přehledovém článku je detailně popsána struktura, metody extrakce, funkční a biologické vlastnosti a možnosti aplikace obou typů hydrokoloidů, zejména pro fortifikaci různých potravin a další potravinářské využití. Na tuto práci navazuje krátký přehledový článek shrnující současné trendy v potravinářských a dietárních aplikacích lněného slizu. První publikace popisuje spíše tradiční využití lněného slizu ve formě obohacování potravin za účelem zvýšení technologické, nutriční či senzorické jakosti potravin, případně poskytnutí zdravotního benefitu. Druhá přehledová publikace se zaměřuje výhradně na možnosti využití lněného slizu v soudobých a inovativních oblastech potravinářského průmyslu a výživy. Lněný sliz lze v rámci moderních aplikací využít například při tvorbě filmů pro ochranu potravin, jako strukturní komponent v oleogelech, kryogelech nebo aerogelech a rovněž také pro modifikaci funkčních vlastností bílkovin v rámci společných komplexů. V rámci výživy je možné uplatnit lnění sliz pro enkapsulaci probiotik nebo biologicky aktivních látek. Lze jej také využít jako prebiotikum pro zvýšení podílu prospěšných probiotických mikroorganismů. Obě přehledové práce v důsledku zdůrazňují významný potenciál bílkovin a slizů lněného semene pro současné i budoucí aplikace různých směrů. Druhá část této práce představuje provoautorská publikace zaměřená na výzkum vlivu odrůdy a pěstitelských podmínek na výnosové parametry, chemické složení, obsah lignanů a antioxidační potenciál lněných semen.
Keywords:
len setý; lněný sliz; bílkoviny; fenolické látky; antioxidační aktivita; funkční vlastnosti; flaxseed; flaxseed mucilage; proteins; phenolic substances; antioxidant activity; functional properties
Available in the Digital Repository of University of South Bohemia.
Characterization of proteins, soluble gums and phenolic compounds isolated from flaxseed (Linum usitatissimum L.)
Flax (Linum usitatissimum L.) is one of the oldest domesticated crops. Its cultivation for fibre, oil or medicinal purposes was already widespread in ancient times. Nowadays, the growing of flax for ...
NRGL provides central access to information on grey literature produced in the Czech Republic in the fields of science, research and education. You can find more information about grey literature and NRGL at service web
Send your suggestions and comments to nusl@techlib.cz
Provider
Other bases