Seeking Novel Nitrilase Enzyme (Nitrile Aminohydrolase, EC 3.5.5.1)
Martínková, Ludmila; Kaplan, Ondřej; Veselá, Alicja Barbara
2014 - anglický
Our research group has created a library of novel nitrilases. In general, the enzymes are available from heterologous producers in amounts ranging from 10 to 20 mg of protein exhbiting 25-50 % purity. The enzymes differ from each other in substrate specificity, temperature optimum, enantioselectivity (arylacetonitrilases) etc. Preferential substrates are phenylacetonitrile, (R,S)-mandelonitrile, benzonitrile or cyanopyridines (depending on the enzyme).
Klíčová slova:
Nitrilase; Expression; Purification
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Seeking Novel Nitrilase Enzyme (Nitrile Aminohydrolase, EC 3.5.5.1)
Our research group has created a library of novel nitrilases. In general, the enzymes are available from heterologous producers in amounts ranging from 10 to 20 mg of protein exhbiting 25-50 % purity. ...
Vývoj technologií lyopfilizace buněčných lyzátů
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2014 - anglický
Development and optimization of lyophilization methods of the cell content avoiding loss of functionality.
Klíčová slova:
lyophyllization; cell content; biotechnology
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Vývoj technologií lyopfilizace buněčných lyzátů
Development and optimization of lyophilization methods of the cell content avoiding loss of functionality.
Microbial and molecular biological analysis of technological solutions from Glanzstoff Bohemia s.r.o
Brennerová, Mária
2014 - anglický
Main goal of the study was identification of the bacterial contaminations during the production of high-tenacity viscose filaments. The work describes microbial cultivations, isolation of bacteria from technological solutions, and taxonomical identification through sequencing of 16S ribosomal RNA genes and searching for affiliations with bacterial relatives in the NCBI website.
Klíčová slova:
viscose; technological solutions; bacterial isolates; identification
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Microbial and molecular biological analysis of technological solutions from Glanzstoff Bohemia s.r.o
Main goal of the study was identification of the bacterial contaminations during the production of high-tenacity viscose filaments. The work describes microbial cultivations, isolation of bacteria ...
Vývoj kultivačních technologií výroby intracelulárních alkoholdehydrogenáz
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2014 - anglický
Development and optimization of pilot cultivations and DSP of intracellular recombinant enzyme productions.
Klíčová slova:
enzyme production; bacterial production; submergeed aerobic cultivation
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Vývoj kultivačních technologií výroby intracelulárních alkoholdehydrogenáz
Development and optimization of pilot cultivations and DSP of intracellular recombinant enzyme productions.
Host interactions and lactobacilli: from fly model to mouse model/ Role of the intestinal lactobacilli in growth promoting in juvenile development
Kozáková, Hana
2014 - anglický
We confirmed the hypothesis that the intestinal flora supports juvenile growth in mice, and that the bacterial isolate is capable and mice recapitulate promote growth, similarly as complete microflora.
Klíčová slova:
flies; lactobacili; mice
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Host interactions and lactobacilli: from fly model to mouse model/ Role of the intestinal lactobacilli in growth promoting in juvenile development
We confirmed the hypothesis that the intestinal flora supports juvenile growth in mice, and that the bacterial isolate is capable and mice recapitulate promote growth, similarly as complete ...
Transfer of technology for production of AEH from Sphingomonas sp
Kyslíková, Eva; Palyzová, Andrea; Marešová, Helena; Štěpánek, Václav; Zahradník, Jiří; Bečka, Stanislav; Dobišová, Marie; Kyslík, Pavel
2014 - anglický
Research report on construction of a recombinant production microorganism, the way of its cultivation in stirred bioreactor, overproduction and isolation of AEH and stabilization of AEH as cross linked enzyme agregate
Klíčová slova:
Alpha aminoacid ester hydrolase; heterologous expression; stirred bioreactor
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Transfer of technology for production of AEH from Sphingomonas sp
Research report on construction of a recombinant production microorganism, the way of its cultivation in stirred bioreactor, overproduction and isolation of AEH and stabilization of AEH as cross ...
Hydrodynamic Study of the Downward Annular Gas-Liquid Flow under the Pilot Plant Operating Conditions 2013-2014.
Stavárek, Petr; Křišťál, Jiří
2014 - anglický
Hydrodynamics of annular liquid flow in a vertical tube with cocurrent gas flow has been studied. Effect of gas - liquid flow rate and liquid viscosity on two-phase flow hydrodynamics was investigated for glycerol – air system. The evaluated parameters were pressure drop and hydrodynamic regime at different gas/liquid ratios.
Klíčová slova:
annular gas-liquid flow; pressure drop; hydrodynamics
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Hydrodynamic Study of the Downward Annular Gas-Liquid Flow under the Pilot Plant Operating Conditions 2013-2014.
Hydrodynamics of annular liquid flow in a vertical tube with cocurrent gas flow has been studied. Effect of gas - liquid flow rate and liquid viscosity on two-phase flow hydrodynamics was investigated ...
New inorganic matrices for pultrusion technology
Černý, Zbyněk
2014 - anglický
Development of new inorganic matrix enabling rapid continuous preparation of thermally and chemically stable fire resistant technological profiles and components with controlled properties.
Klíčová slova:
pultrusion technology; inorganic matrice
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
New inorganic matrices for pultrusion technology
Development of new inorganic matrix enabling rapid continuous preparation of thermally and chemically stable fire resistant technological profiles and components with controlled properties.
18M-IMMEDIATE Research Report: Prototype of Catalyst Powder Sufficient for Testing in MEAs Fabricated
Kaluža, Luděk; Zdražil, Miroslav; Gulková, Daniela; Koštejn, Martin; Vít, Zdeněk
2014 - anglický
High loadings of platinum (60 wt%) were precipitated (ICPF) or depo-sited (CNRS) onto research grade carbon black supports provided by TC. The supports and the resulting electrocatalysts were thoroughly characterised in order to select the best ones for optimisation and up-scaled manufacturing. For the preparation and characterisation vari-ous methodologies have been developed. Batches of the gram size were prepared of a handful of the most promising catalysts. This is sufficient for the preparation of test MEAs.
Klíčová slova:
Pt catalysts; stress tests; nitrogen physisorption
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
18M-IMMEDIATE Research Report: Prototype of Catalyst Powder Sufficient for Testing in MEAs Fabricated
High loadings of platinum (60 wt%) were precipitated (ICPF) or depo-sited (CNRS) onto research grade carbon black supports provided by TC. The supports and the resulting electrocatalysts were ...
SMV-2014-732: Preparation, testing and delivery of isoelectric point standards
Šlais, Karel
2014 - anglický
The low molecular mass pI markers were prepared, examined and delivered with aim to fill up the previously described pI values. The pI markers comprise compounds based on our original previously described aminomethylphenols. The prepared compounds are the good ampholytes with high hydrophility and molar absorbtivity.
Klíčová slova:
Isoelectric focusing; low molecular mass pI markers; aminomethylphenols
Plné texty jsou dostupné na vyžádání prostřednictvím repozitáře Akademie věd.
SMV-2014-732: Preparation, testing and delivery of isoelectric point standards
The low molecular mass pI markers were prepared, examined and delivered with aim to fill up the previously described pI values. The pI markers comprise compounds based on our original previously ...
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