Speciation analysis employing hydride generation
Matoušek, Tomáš
2023 - Czech
The lecture presented employing of different ways of hydride generation- selective generation, postcolumn generation and cryotrapping- useful of element speciation analysis. Examples from our laboratory covered applications from the field of clinical, food and natural water analysis. Přednáška prezentovala různé způsoby využití postupu generování hydridů- selektivní generování, postkolonové generování a generování s kryoseparací- v prvkových speciačních analýzách. Příklady z naší laboratoře zahrnovaly aplikace z oblasti klinické analýzy, analýzy potravin a přírodních vod.
Keywords:
hydrides generation; inductively coupled plasma mass spectrometry; speciation analysis
Available on request at various institutes of the ASCR
Speciation analysis employing hydride generation
The lecture presented employing of different ways of hydride generation- selective generation, postcolumn generation and cryotrapping- useful of element speciation analysis. Examples from our ...
Volatile species generation – an efficient sample introduction technique for ICP-MS
Musil, Stanislav
2023 - Czech
Various techniques of volatile species generation, the ways of coupling to ICP-MS and recent results of our laboratory in the field of photochemical vapor generation of transition metals were presented in this lecture. V rámci této přednášky byly prezentovány různé techniky generování těkavých sloučenin (specií), možnosti spojení s ICP-MS a nedávné výsledky naší laboratoře v oblasti fotochemického generování těkavých specií přechodných prvků.
Keywords:
volatile species generation; inductively coupled plasma mass spectrometry; photochemical vapor generation
Available at various institutes of the ASCR
Volatile species generation – an efficient sample introduction technique for ICP-MS
Various techniques of volatile species generation, the ways of coupling to ICP-MS and recent results of our laboratory in the field of photochemical vapor generation of transition metals were ...
Detection and identification of chemical warfare agent simulants and degradation products by means of portable liquid chromatograph
Vaněk, J.; Šesták, Jozef; Lunerová, K.; Tanzinger, L.
2022 - Czech
The methodology utilizes a portable liquid chromatograph for rapid detection and identification of selected chemical warfare agent simulants and degradation products. It is primarily designated for SÚJCHBO, v. v. i. to control compliance with the law 19/1997 Sb. and for research activity. Metodika využívá přenosný kapalinový chromatograf pro rychlou detekci a kvantifikaci rozkladných produktů BChL a simulantů BChL. Je určena zejména pro potřeby SÚJCHBO, v. v. i. v rámci činností v oblasti VaV a zkušebnictví a při kontrolní a dozorové činnosti v oblasti dodržování Zákona č. 19/1997 Sb.
Keywords:
chemical warfare agent simulants; chemical warfare agent degradation products; portable liquid chromatograph
Available on request at various institutes of the ASCR
Detection and identification of chemical warfare agent simulants and degradation products by means of portable liquid chromatograph
The methodology utilizes a portable liquid chromatograph for rapid detection and identification of selected chemical warfare agent simulants and degradation products. It is primarily designated for ...
Detection and identification of aflatoxins and microcystins by means of portable liquid chromatograph for purpose of control of biological weapons prohibition
Lunerová, K.; Šesták, Jozef
2022 - Czech
The methodology utilizes a portable liquid chromatograph for rapid detection and identification of selected aflatoxins and microcystins. It is primarily designated for SÚJCHBO, v. v. i. to control the compliance with the law 281/2002 Sb. Metodika využívá přenosný kapalinový chromatograf pro rychlou detekci a identifikaci vybraných aflatoxinů a mikrocystinů a je určena zejména pro potřeby SÚJCHBO, v. v. i. při kontrolní a dozorové činnosti v oblasti dodržování Zákona č. 281/2002 Sb.
Keywords:
microcystins; aflatoxins; portable liquid chromatograph
Available on request at various institutes of the ASCR
Detection and identification of aflatoxins and microcystins by means of portable liquid chromatograph for purpose of control of biological weapons prohibition
The methodology utilizes a portable liquid chromatograph for rapid detection and identification of selected aflatoxins and microcystins. It is primarily designated for SÚJCHBO, v. v. i. to control the ...
Portable liquid chromatograph Portable as a new tool for the determination of hazardous substances
Šesták, Jozef; Lunerová, K.; Vaněk, J.
2021 - Czech
The portable liquid chromatograph is a new instrument of compact dimensions, which enables the implementation of HPLC analyzes in conditions outside the laboratory. It was used for the development of a certified methodology for the detection and determination of selected mycotoxins in complex samples.\n\n Přenosný kapalinový chromatograf představuje nový přístroj kompaktních rozměrů, který umožňuje realizaci HPLC analýz v podmínkách mimo laboratoř. Byl využit pro vývoj certifikované metodiky pro detekci a stanovení vybraných mykotoxinů v komplexních vzorcích.
Keywords:
portable liquid chromatograph; mykotoxins; miniaturization
Available on request at various institutes of the ASCR
Portable liquid chromatograph Portable as a new tool for the determination of hazardous substances
The portable liquid chromatograph is a new instrument of compact dimensions, which enables the implementation of HPLC analyzes in conditions outside the laboratory. It was used for the development of ...
Epitachophoretic preconcentration of proteins
Hrušková, Helena; Voráčová, Ivona; Foret, František
2021 - English
In this work, we present a developed method for preconcentration of standards of proteins cytochrome c, myoglobin, and hemoglobin. Firstly, the electrolyte system was selected and optimized to provide low analysis time, narrow shape of the protein zone, and eliminate overheating at the center of the device. In the next step, the developed method was tested on the preconcentration of proteins: cytochrome c, myoglobin, and hemoglobin. After several adjustments myoglobin and hemoglobin were also uccessfully preconcentrated. After evaluation of pilot results, this technique provides up to fiftyfold preconcentration and recovery of over 70 % for each protein. The next plan is to preconcentrate proteins from complex biological matrices such as urine or blood plasma.
Keywords:
epitachophoresis; isotachophoresis; preparative concentration
Available on request at various institutes of the ASCR
Epitachophoretic preconcentration of proteins
In this work, we present a developed method for preconcentration of standards of proteins cytochrome c, myoglobin, and hemoglobin. Firstly, the electrolyte system was selected and optimized to provide ...
Quantum dot luminescent probe for Caspase 3/7 imaging inside cells
Procházková, Markéta; Klepárník, Karel
2021 - English
Two step synthesis of luminescent probe based on ligand-exchange in the first step and the specific reaction of amino group in the second step was optimized. The luminescence properties of the final product were checked by time course of\nactive recombinant caspase protein reaction under the model conditions in fluorimeter. The novel luminescent probe enables long-time imaging of active caspases in living cells or tissues.
Keywords:
caspase; fluorescence; quantum dot
Available on request at various institutes of the ASCR
Quantum dot luminescent probe for Caspase 3/7 imaging inside cells
Two step synthesis of luminescent probe based on ligand-exchange in the first step and the specific reaction of amino group in the second step was optimized. The luminescence properties of the final ...
Detection and identification of aflatoxins by means of portable liquid chromatograph for purpose of control of biological weapons prohibition
Šesták, Jozef; Lunerová, K.
2020 - Czech
Method describes screening detection and identification of selected mycotoxins aflatoxins (aflatoxins B1, B2, G1 and G2), ochratoxins A, B and zearalenon by means of portable miniaturzed capillary liquid chromatograph (miniLC) with UV/VIS and fluorescence detector (FLD). The aim of the method is to provide rapid screening method for finding/excluding the presence of given mycotoxins in unknown samples. In case of positive finding, it is necessary to perform confirmation analysis using other instrumental methods (e. g. LC-MS). The method is intended namely for the needs of technical support of the State Office for Nuclear Safety for control and supervising activities in field of execution of the Act No. 281/1997 Coll (Biological Act). Metodika popisuje způsob screeningové detekce a identifikace vybraných mykotoxinů aflatoxinů (aflatoxinů B1, B2, G1 a G2), ochratoxinů A, B a zearalenonu pomocí přenosného miniaturizovaného kapalinového chromatografu (miniLC) s UV/VIS a fluorescenčním detektorem (FLD). Cílem metodiky je poskytnout rychlou metodu pro zjištění/vyloučení přítomnosti uvedených mykotoxinů v neznámém vzorku. V případě pozitivního zjištění je nutné u daného vzorku provést potvrzení identity látek pomocí dalších instrumentálních metod (např. LC-MS). Metodika je určena zejména pro potřeby technické podpory SÚJB při kontrolní a dozorové činnosti v oblasti dodržování Zákona č. 281/1997 Sb.
Keywords:
miniaturized capillary chromatograph; aflatoxins; portable liquid chromatograph
Available on request at various institutes of the ASCR
Detection and identification of aflatoxins by means of portable liquid chromatograph for purpose of control of biological weapons prohibition
Method describes screening detection and identification of selected mycotoxins aflatoxins (aflatoxins B1, B2, G1 and G2), ochratoxins A, B and zearalenon by means of portable miniaturzed capillary ...
Photon-upconversion nanoparticles for single-molecule immunosensing of cancer biomarkers and bacteria
Farka, Z.; Mickert, M. J.; Hlaváček, Antonín; Poláchová, V.; Kostiv, Uliana; Pastucha, M.; Horák, Daniel; Gorris, H. H.; Skládal, P.
2020 - English
The recent progress in the field of immunoassays has been driven by introduction of various kinds of nanomaterials. In particular, photon-upconversion nanoparticles (UCNPs) proved to be excellent immunoassay labels due to their ability to emit light of shorter wavelengths under near-infrared excitation (anti-Stokes emission), which prevents autofluorescence, minimizes light scattering, and thus reduces the optical background interference. These unique photoluminescent properties allow counting of individual biomolecules labeled with UCNPs by conventional wide-field epiluminescence microscopy and enable the development of single-molecule (digital) immunoassays. We have introduced a novel label based on UCNPs conjugated with streptavidin via poly(ethylene glycol) and applied it in a digital upconversion-linked immunosorbent assay (ULISA) for the detection of a cancer biomarker prostate specific antigen (PSA). The digital readout based on counting of individual immunocomplexes improved the sensitivity 16× compared to conventional analog readout and allowed to reach a limit of detection (LOD) of 23 fg·mL−1 (800 aM). Human serum samples were successfully analyzed achieving an excellent correlation with electrochemiluminescence reference method. The conjugates of UCNPs with streptavidin are also suitable for the detection of pathogenic bacterium Melissococcus plutonius, the causative agent of honeybee disease European foulbrood. The ULISA assay provided an LOD of 340 CFU·mL−1 and successfully analyzed real samples of bees, larvae and bottom hive debris. Due to the high reliability and relatively simple detection scheme, the digital ULISA can pave the way for a new generation of digital immunoassays with a strong potential for commercialization.
Keywords:
single-molecule detection; photon-upconversion nanoparticle; bioconjugation
Available at various institutes of the ASCR
Photon-upconversion nanoparticles for single-molecule immunosensing of cancer biomarkers and bacteria
The recent progress in the field of immunoassays has been driven by introduction of various kinds of nanomaterials. In particular, photon-upconversion nanoparticles (UCNPs) proved to be excellent ...
Chemical vapor generation of cadmium for analytical atomic spectrometry
Sagapova, Linda; Kodríková, Barbora; Svoboda, Milan; Musil, Stanislav; Kratzer, Jan
2020 - English
Chemical vapor generation of cadmium volatile compounds was optimized in order to determine trace Cd concentrations by atomic absorption spectrometry (AAS). Several reaction modifiers based on inorganic salts and complexes of CrIII+, CoII+, TiIII+, TiIV+ were tested to improve analytical performance and generation efficiency.
Keywords:
chemical vapor generation; cadmium; atomic absorption spectrometry
Available on request at various institutes of the ASCR
Chemical vapor generation of cadmium for analytical atomic spectrometry
Chemical vapor generation of cadmium volatile compounds was optimized in order to determine trace Cd concentrations by atomic absorption spectrometry (AAS). Several reaction modifiers based on ...
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