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Speciation analysis of As and Hg in biological material
Petry-Podgórska, Inga; Matoušek, Tomáš; Migašová, M.; Zemanová, Veronika; Pavlík, Milan; Pavlíková, D.; Kratzer, Jan
2018 - anglický
This work was focused on an analysis of arsenic in selected plants and mercury in hair. A combination of HPLC and ICP-MS was used for analysis of biological tissues extracts. The\nspeciation analysis of low molecular arsenic and mercury species like inorganic iAs3+ and iAs5+, methylarsenic and dimethylarsenic forms was carried out with hydride generation (HG)\ntechnique, arsenic peptides and inorganic mercury (iHg2+) and its methylated form (MHg+) - with reverse phase (RP) chromatography and detected by ICP-MS.
Klíčová slova:
mercury; arsenic; speciation
Dokument je dostupný na externích webových stránkách.
Speciation analysis of As and Hg in biological material
This work was focused on an analysis of arsenic in selected plants and mercury in hair. A combination of HPLC and ICP-MS was used for analysis of biological tissues extracts. The\nspeciation analysis ...
Synthetic approaches for new labeling tags facilitating glycan analysis
Čmelík, Richard; Křenková, Jana; Foret, František
2018 - anglický
This study is focused on the synthesis of labels based on the pyrene core bearing several ionizable quaternary amines and the efficient carbonyl-reactive group, such as primary amine or hydrazine.
Klíčová slova:
labeling; organic synthesis; glycan
Dokument je dostupný na externích webových stránkách.
Synthetic approaches for new labeling tags facilitating glycan analysis
This study is focused on the synthesis of labels based on the pyrene core bearing several ionizable quaternary amines and the efficient carbonyl-reactive group, such as primary amine or hydrazine.
Evaluation of the 33 synthesized nitrophenol-based markers of isoelectic point
Duša, Filip; Moravcová, Dana; Šlais, Karel
2018 - anglický
33 nitrophenol-based compounds, which absorb at ultraviolet as well as visible wavelengths, were selected as candidates for marking isoelectric points (pI) over the pH range from 3 to 10. The evaluation and selection of the candidate structures included structure-based calculations of acid-base properties. The selected compounds were further tested experimentally using capillary isoelectric focusing. The experimental analysis excluded two compounds due to producing double peaks during the analysis. Overall, 31 novel compounds were identified as suitable pI markers.
Klíčová slova:
nitrophenol-based markers; isoelectic point; capillary isoelectric focusing
Dokument je dostupný na externích webových stránkách.
Evaluation of the 33 synthesized nitrophenol-based markers of isoelectic point
33 nitrophenol-based compounds, which absorb at ultraviolet as well as visible wavelengths, were selected as candidates for marking isoelectric points (pI) over the pH range from 3 to 10. The ...
Chromatographic separation of aflatoxin analogues
Moravcová, Dana; Planeta, Josef; Lunerová, K.; Duša, Filip; Šesták, Jozef; Kubíček, J.; Horká, Marie
2018 - anglický
Three short particle-packed columns (50 x 2.1 mm) were compared for the rapid and efficient chromatographic separation of aflatoxin G1, G2, B1 and B2 analogues.
Klíčová slova:
aflatoxin; HPLC; C18 stationary phase
Dokument je dostupný na externích webových stránkách.
Chromatographic separation of aflatoxin analogues
Three short particle-packed columns (50 x 2.1 mm) were compared for the rapid and efficient chromatographic separation of aflatoxin G1, G2, B1 and B2 analogues.
Arduino-based pressure-driven flow controller for droplet microfluidics
Přikryl, Jan; Křivánková, Jana; Hlaváček, Antonín
2018 - anglický
Droplet-based microfluidic systems bear significant value in an extremely wide range of applications due to its remarkable advantages, nevertheless, their technical demands on precise flow control are high. Here, we present lab-built Arduino-based flow controller fulfilling needs of droplet microfluidics to easily and effectively automate the system. As an additional required accessory, CNC-milled component for vials pressurizing was presented.
Klíčová slova:
droplet microfluidics; microfluidic systems; pressure regulator
Dokument je dostupný na externích webových stránkách.
Arduino-based pressure-driven flow controller for droplet microfluidics
Droplet-based microfluidic systems bear significant value in an extremely wide range of applications due to its remarkable advantages, nevertheless, their technical demands on precise flow control are ...
Rapid fabrication of microextraction devices for direct determination of basic drugs in dried blood spots
Ryšavá, Lenka; Přikryl, Jan; Dvořák, Miloš; Malá, Zdeňka; Kubáň, Pavel
2018 - anglický
A 3D-printed cutter was designed for rapid fabrication of supported liquid membrane (SLM) extraction devices compatible with injection system of commercial capillary electrophoresis (CE) instrument. Low cost and single use microextraction devices were fabricated from polypropylene micropipette tips with high precision (dimension variances ≤ 3% RSD). Practical applicability of the microextraction devices was examined by in-line coupling of SLM extractions to CE for direct analysis of four model basic drugs in dried blood spot extracts. Excellent repeatability of the hyphenated technique was achieved (RSD ≤ 7.6%), transfer of analytes across the SLM ranged from 23 to 73% and limits of detection were as low as 0.07 μg/mL.
Klíčová slova:
supported liquid membrane (SLM); capillary electrophoresis; microextraction devices
Dokument je dostupný na externích webových stránkách.
Rapid fabrication of microextraction devices for direct determination of basic drugs in dried blood spots
A 3D-printed cutter was designed for rapid fabrication of supported liquid membrane (SLM) extraction devices compatible with injection system of commercial capillary electrophoresis (CE) instrument. ...
Maximizing efficiency of field-amplified electrokinetic injection of weak bases for enantioselective capillary electrophoresis with sulfated cyclodextins
Šesták, Jozef; Thormann, W.
2018 - anglický
The optimal conditions for field-amplified electrokinetic injection of cations followed by their separation based on the stereoselective interaction with sulfated cyclodextrins are discussed. Results show that addition of a short water plug together with a short plug free of cyclodextrins and with a buffer concentration similar to the BGE greatly increase the injection efficiency.
Klíčová slova:
capillary electrophoresis; cyclodextrins
Dokument je dostupný na externích webových stránkách.
Maximizing efficiency of field-amplified electrokinetic injection of weak bases for enantioselective capillary electrophoresis with sulfated cyclodextins
The optimal conditions for field-amplified electrokinetic injection of cations followed by their separation based on the stereoselective interaction with sulfated cyclodextrins are discussed. Results ...
A New evaluation of combined antitumor effects of natural and synthetic nuclear retinoid receptor ligands in human breast carcinoma cells
Strouhalová, Dana; Laštovičková, Markéta; Bobál, P.; Macejová, D.; Mosná, B.; Brtko, J.; Bobálová, Janette
2018 - anglický
This work represents the study of the effects of biologically active ligands of nuclear retinoid X receptors, triorganotin compounds, on proteomic profile of MDA-MB-231 cells. We focused primarily on tributyl/phenyltin derivatives that suggest the combined antitumor effect when they are used with natural nuclear retinoid receptor ligand all-trans retinoic acid (ATRA). Proteomic strategies connected to PDQuest evaluation were applied in this study. Some specific proteins with tumor process association were chosen for more detail study. Our findings indicate that selected triorganotins derivatives resulted to significantly reduced level of studied proteins belonging to metabolic pathway or to other cellular processes as apoptosis or epithelial–mesenchymal transition. In the case of vimentin, the key protein of EMT process, the silencing was set up by triorganotin compounds and significantly enhanced by combination with ATRA. This additive effect on downregulation can result from the potential engagement of RXR/RAR heterodimer, considering co-addition of both partner ligands of mentioned heterodimer to the treatment.
Klíčová slova:
nuclear retinoid X receptors; triorganotin compounds; breast carcinoma cells
Dokument je dostupný na externích webových stránkách.
A New evaluation of combined antitumor effects of natural and synthetic nuclear retinoid receptor ligands in human breast carcinoma cells
This work represents the study of the effects of biologically active ligands of nuclear retinoid X receptors, triorganotin compounds, on proteomic profile of MDA-MB-231 cells. We focused primarily on ...
SMV-2017-743: Preparation, testing and delivery of isoelectric point standards
Čmelík, Richard
2017 - anglický
The project continues long-term cooperation of both participants. The low-molecular mass pI markers were synthesized and examined with aim to fill up and deliver the previously described compounds.
Klíčová slova:
Isoelectric focusing; pI markers; aminomethylphenols
Plné texty jsou dostupné na vyžádání prostřednictvím repozitáře Akademie věd.
SMV-2017-743: Preparation, testing and delivery of isoelectric point standards
The project continues long-term cooperation of both participants. The low-molecular mass pI markers were synthesized and examined with aim to fill up and deliver the previously described compounds.
SMV-2017-732: Preparation, testing and delivery of isoelectric point standards
Čmelík, Richard
2017 - anglický
The project continues long-term cooperation of both participants. The low-molecular mass pI markers were synthesized and examined with aim to fill up and deliver the previously described compounds.
Klíčová slova:
Isoelectric focusing; pI markers; aminomethylphenols
Plné texty jsou dostupné na vyžádání prostřednictvím repozitáře Akademie věd.
SMV-2017-732: Preparation, testing and delivery of isoelectric point standards
The project continues long-term cooperation of both participants. The low-molecular mass pI markers were synthesized and examined with aim to fill up and deliver the previously described compounds.
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