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Microbial degradation of 17alfa-ethinylestradiol and bisphenol A
Křesinová, Zdena; Svobodová, Kateřina; Moeder, M.; Cajthaml, Tomáš
2009 - Czech
17ethinylestradiol (EE2) and bisphenol A (BA) are endocrine disrupting compounds (ED). ED have a negative influence on animals and human hormonal systems animals, and thus they are serious envronmental contaminants. This work was focused on biodegradtion of EE2 and BA using 8 white rot fungi strains in liquid media. Pleurotus ostreatus, Pycnoporus cinnabarinus and Irpex lacteus were able to remove both ED under detection limit (initial concetration was 10 ppm) during the first three days of cultivation. In contrary, Phanerochaete chrysosporium was not able to degrade both ED durin 2 weaks long cultivation 17 ethinylestradiol (EE2) a bisfenol A (BA) patří mezi skupinu látek označovaných jako endokrinní disruptory (ED). Tyto látky mají negativní vliv na hormonální systém živočichů a člověka, a představují závažný problém z hlediska kontaminace životního prostředí. Tato práce byla zaměřená na biodegradace uvedených látek pomocí osmi druhů hub bílé hniloby v tekutých médiích. Již během prvních tří dnů kultivace se zástupci kmenů Pleurotus ostreatus, Pycnoporus cinnabarinus a Irpex lacteus poklesla koncentrace obou ED z původní koncentrace10 ppm pod limit detekce. Naopak v experimentu s Phanerochaete chrysosporium nebyl zaznamenán pokles koncentrace EE2 a BA ani po dvou týdnech kultivace. Během degradačního pokusu byl dále měřen vliv přítomnosti EE2 a BA na expresi ligninolytických enzymů hub bílé hniloby (lakasa, manganperoxidasa a ligninperoxidasa), které by mohly participovat na biodegradačních procesech
Keywords:
ligninolytic fungi; biobiodegradation; endocrine disrupting compounds
Available at various institutes of the ASCR
Microbial degradation of 17alfa-ethinylestradiol and bisphenol A
17ethinylestradiol (EE2) and bisphenol A (BA) are endocrine disrupting compounds (ED). ED have a negative influence on animals and human hormonal systems animals, and thus they are serious ...
CHARACTERIZATION OF MICROBIAL POPULATIONS DURING COMPOSTING OF CONTAMINATED SOIL
Petránková, Mirka; Čechová, Zuzana; Brennerová, Mária; Cajthaml, Tomáš
2009 - Czech
Bioremediation is an indisputably advantageous approach to cleaning up contaminated soils. The efficiency of bioremediation depends on a number of factors, the most important of which are the ability of the bioremediative organism to degrade the target pollutants, the pollutant’s intrinsic properties (aqueous solubility being of key importance), and soil parameters. Composting has been recently shown to be efficient for remediation of soils contaminated e.g. with PAHs Bioremediace je jedna z nejvýhodnějších metod pro dekontaminaci znečištěných půd. Její účinnost závisí na několika faktorech, nejvíce však na schopnosti bioremediačních organismů degradovat cílové polutanty, na fyzikálně-chemických vlastnostech polutantů (např. rozpustnost ve vodě) a na vlastnostech půdy. Kompostování se ukazuje jako vhodná technika pro remediaci půd kontaminovaných např. PAU. Proces je založen na schopnosti mikrorganismů degradovat organický materiál v závislosti na termogenezi. Teplota uvnitř kompostovací jednotky může dosáhnout až 75 stupňů C. Pro remediaci je kompostovací substrát jednoduše smíchán s částí kontaminované půdy
Keywords:
Phospholipid fatty acids; biodegradation; composting
Available at various institutes of the ASCR
CHARACTERIZATION OF MICROBIAL POPULATIONS DURING COMPOSTING OF CONTAMINATED SOIL
Bioremediation is an indisputably advantageous approach to cleaning up contaminated soils. The efficiency of bioremediation depends on a number of factors, the most important of which are the ability ...
Degradation of lignin and humic substances in forest soil by saprotrophic basidiomycetes
Šnajdr, Jaroslav; Steffen, K. T.; Hofrichter, M.; Baldrian, Petr
2009 - English
The work deals of degradation of lignin and humic substances in forest soil by saprotrophic basidiomycetes is significant, yet species-dependent
Keywords:
soil; enzyme; interaction
Available at various institutes of the ASCR
Degradation of lignin and humic substances in forest soil by saprotrophic basidiomycetes
The work deals of degradation of lignin and humic substances in forest soil by saprotrophic basidiomycetes is significant, yet species-dependent
SILVER NANOPARTICLES AND THEIR BACTERICIDAL EFFECT
Piksová, K.; Weiserová, Marie; Jedličková, A.; Fojtík, A.
2009 - English
Rapid development of bio-nanotechnology lead to the new way in the combating of bacteria and to searching specific properties of nanomaterials The study of bactericidal nanomaterials is particularly timely considering the recent increase of new resistant strains of bacteria to the most potent antibiotics The present work studies the bactericidal effect of silver nanoparticles in the range of 10-30 nm on Gram-negative bacteria and Gram-positive bacteria
Keywords:
silver nanoparticles; bactericidal effect
Available at various institutes of the ASCR
SILVER NANOPARTICLES AND THEIR BACTERICIDAL EFFECT
Rapid development of bio-nanotechnology lead to the new way in the combating of bacteria and to searching specific properties of nanomaterials The study of bactericidal nanomaterials is particularly ...
Comparison of in vitro tests for measurement of estrogenity of chlorinated compounds and their use in the evaluation of biodegradation of chlorbenzoates by the fungus Panus tigrinus
Svobodová, Kateřina; Covino, S.; Křesinová, Zdena; Cajthaml, Tomáš
2009 - English
Recently, contamination of the environment by endocrine disruptive compounds, toxicity of these chemicals and their biodegradability are widely studied. This work is focused on estrogenity of chlorinated phenols and chlorbenzoic acids that represent potential degradation products of PCB compounds. Two different yeast bioassays for evaluation of estrogenity based on recombinated strains of Saccharomyces cerevisie were compared in this work. Estrogenic activity of chlorbenzoic acids varied with the chemical structure of chemicals. A mixture of chlorbenzoates (initial concentration 10 ppm) was degraded by a ligninolytic fungus Panus tigrinus in liquid cultures. Chemicals resulting from degradation were tested for their estrogenity, however, results indicated that the test responses were inhibited probably by some compounds present in the fungal culture liquid
Keywords:
yeast assay; biodegradation; chlorbenzoates
Available at various institutes of the ASCR
Comparison of in vitro tests for measurement of estrogenity of chlorinated compounds and their use in the evaluation of biodegradation of chlorbenzoates by the fungus Panus tigrinus
Recently, contamination of the environment by endocrine disruptive compounds, toxicity of these chemicals and their biodegradability are widely studied. This work is focused on estrogenity of ...
Screening of fungi are able to degradation of chlorobenzoates
Muzikář, Milan; Křesinová, Zdena; Svobodová, Kateřina; Cajthaml, Tomáš
2009 - Czech
Degradation of chlorobenzoates (CBA), which are the intermediates by degradation of polychlorinated biphenyls, with fungi capable degrade lignin. These fungi are called ligninolytic or white-rot fungi. Biodegradation was tested in rich medium. The cultures were Bjerkandera adusta, Dichomitus squalens, Irpex lacteus, Phanerochaete chysosporium, Pleurotus ostreatus, Pycnoporus cinnabarinus, Pycnoporus magnoliae a Trametes versicolor. The harvesting was performance 1., 2. and 3. week. Via ethylacetate we obtained the organic extract which was analyzed on HPLC. The start concentration was 10 ppm (10 μg/ml). We tested 12 substances of CBA Degradace chlorbenzoátů (CBA), meziproduktů při odbourávání polychlorovaných bifenylů (PCB), pomocí hub schopných odbourávat lignin. Tyto houby se nazývají ligninolytické nebo houby bílé hniloby. Biodegradace byla testována v bohatém médiu. Měřené kultury Bjerkandera adusta, Dichomitus squalens, Irpex lacteus, Phanerochaete chysosporium, Pleurotus ostreatus, Pycnoporus cinnabarinus, Pycnoporus magnoliae a Trametes versicolor byly odebírána v časových intervalech a organický extrakt byl posléze analyzován na HPLC. Časové intervaly byly 1, 2 a 3 týdny. Zvolená počáteční koncentrace CBA byla 10 ppm (10 μg/ml). Bylo sledováno 12 druhů látek ze skupiny CBA. 2,6 – CBA se v médiu vyskytovala téměř ve stejné koncentraci jako na začátku pokusu. Stejného výsledku se dosáhlo i u více chlorovaných CBA
Keywords:
degradation; ligninolytic fungi; chlorobenzoates
Available at various institutes of the ASCR
Screening of fungi are able to degradation of chlorobenzoates
Degradation of chlorobenzoates (CBA), which are the intermediates by degradation of polychlorinated biphenyls, with fungi capable degrade lignin. These fungi are called ligninolytic or white-rot ...
Biodegradation of PCBs by a ligninolytic fungus
Cajthaml, Tomáš; Kožená, Lenka; Filipová, Alena; Svobodová, Kateřina
2009 - Czech
Although production and use of polychlorinated biphenyls has been prohibited for many years, the compounds still represent a serious problem for the environment. The objective of this work was to test abilities of PCB degradation of several representatives of ligninolytic fungi: Phanerochaete chrysosporium, Pleurotus ostreatus, Trametes versicolor, Irpex lacteus, Bjerkandera adusta, Picnoporus cinnabarinus, Dichomitus squalens, Phanerochaete magnolia, Panus tigrinus in two types of nutrient media i.e. low nitrogen mineral and complex. Ačkoliv výroba používání polychlorovaných bifenylů (PCB) je již řadu desetiletí zakázána, stále tyto persistentní polutanty představují významné riziko z hlediska životního prostředí. V rámci této práce bylo testováno 9 zástupců perspektivních kmenů ligninolytických hub pro biodegradace PCB: Phanerochaete chrysosporium, Pleurotus ostreatus, Trametes versicolor, Irpex lacteus, Bjerkandera adusta, Picnoporus cinnabarinus, Dichomitus squalens, Phanerochaete magnolia, Panus tigrinus, ve dvou typech živných medií: dusíkem limitovaném a bohatém komplexním
Keywords:
degradation; ligninolytic fungi; pcb
Available at various institutes of the ASCR
Biodegradation of PCBs by a ligninolytic fungus
Although production and use of polychlorinated biphenyls has been prohibited for many years, the compounds still represent a serious problem for the environment. The objective of this work was to test ...
Enzyme-catalysed processes in the spoil heaps after brown coal mining: effects of site age, vegetation and seasonality
Baldrian, Petr; Šnajdr, Jaroslav; Valášková, Vendula; Urbanová, Michaela; Voříšková, Jana; Popelářová, Petra; Herinková, Jana; Cajthaml, Tomáš; Frouz, Jan
2009 - English
Enzyme-catalysed processes in the spoil heaps after brown coal mining are affected by site age, vegetation composition and seasonality
Keywords:
soil; enzyme; succesion
Available at various institutes of the ASCR
Enzyme-catalysed processes in the spoil heaps after brown coal mining: effects of site age, vegetation and seasonality
Enzyme-catalysed processes in the spoil heaps after brown coal mining are affected by site age, vegetation composition and seasonality
Comparison of ecotoxicity and persistent organic pollutant bioavailability in real contaminated soil, using selective supercritical fluid extraction (SFE)
Čvančarová, Monika; Křesinová, Zdena; Cajthaml, Tomáš
2009 - Czech
The amount of bioavailable polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs) in contaminated soil was estimated using Supercritical fluid extraction (SFE). The toxicity of contaminated soil was determinated using different organisms: earthworms Eisenia fetida, duckweed Lemna minor, crustacean Heterocypris incongruens, bioluminiscent bacterium Vibrio fischeri and worms Enchytraeus crypticus. There was a different amount of bioavailable fraction in each soil. There was close relationship between ecotoxicity and bioavailability in PAHs contaminated soil Metoda sekvenční nadkritické fluidní extrakce (SFE) je vhodným nástrojem pro odhad biodostupnosti organopolutantů v půdě. Byly stanoveny biodostupné frakce organopolutantů v několika půdách kontaminovaných PCB a PAU
Keywords:
soil; bioavailability; sfe
Available at various institutes of the ASCR
Comparison of ecotoxicity and persistent organic pollutant bioavailability in real contaminated soil, using selective supercritical fluid extraction (SFE)
The amount of bioavailable polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs) in contaminated soil was estimated using Supercritical fluid extraction (SFE). The toxicity of ...
Microalgae as a feedstock for production of bioethanol
Doucha, Jiří; Lívanský, Karel; Doušková, Irena; Zachleder, Vilém
2008 - English
Mnoho zemí aktivně vyvíjí program produkce biopaliv. Vedoucí postavení má v tomto směru Brazílie, kde současnou spotřebu benzínu ze 30 % pokrývá etanol vyráběný relativně levně z cukrové třtiny. Ve vyspělých zemích se bioetanol (3-5 % současné spotřeby benzínu) vyrábí především z kukuřice (USA) nebo z pšenice (EU). Nicméně produkce etanolu z obilnin je drahá. Slibnou surovinu představují mikroskopické řasy. Některé mohou překročit výtěžky zemědělských plodin 10–15 krát. Technologie růstu jednobuněčných řas v tenké vrstvě na nakloněných rovinách je porovnána se světově rozšířeným způsobem kultivace v otevřených bazénech. Bylo zjištěno, že díky vyšším výtěžkům a nižší spotřebě energie, jsou náklady na výrobu biomasy v tenkovrstvých reaktorech pouze 20 % v porovnání s otevřenými bazény Many countries are actively developing biofuels program. Leading in this field is Brazil whose present need of gasoline is supplied with 30 % by ethanol produced relatively cheaply from sugarcane. In developed countries bioethanol (3-5 % of present gasoline need) is produced mostly from corn (USA) or wheat (EU). However, ethanol production from cereals is expensive. Promising feedstock are microalgae. Some of them can surpass yields of classical crop 10-15 times. Technology of growth of unicellular Chlorella in very thin layer on inclined areas is compared with the technology of world-wide mode of cultivation in open ponds. It was found, that due to higher yields and lower energy needs, the cost of biomass production in thin-layer bioractores is only 20 % compared with ponds
Keywords:
biomass; microalgae; bioethanol
Available at various institutes of the ASCR
Microalgae as a feedstock for production of bioethanol
Mnoho zemí aktivně vyvíjí program produkce biopaliv. Vedoucí postavení má v tomto směru Brazílie, kde současnou spotřebu benzínu ze 30 % pokrývá etanol vyráběný relativně levně z cukrové třtiny. Ve ...
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