Bacteriophage 936 by qPCR quantification in dairy products.
Olša Fliegerová, Kateřina; Mrázek, Jakub; Štrosová, Lenka; Němečková, I.; Kopečný, Jan
2018 - Czech
Virulent bacteriophages are still representing significant threat in dairy production even with frequent disinfection and sterilization all the equipment in dairy plants. Therefore a reliable \nmethod of phage monitoring and quantification necessary part of phage prevention. Method is based on qPCR amplification and fluorescent detection of copies number of the phage main\nstructural protein and therefore is independent on the phage cultivation. It is quick, reliable a sensitive enough to estimate phage copy numbers in dairy starters and whey in any phase of dairy production. \n Virulentní bakteriofágy stále představují vážnou hrozbu pro průmyslové fermentace mléka, a to i při časté dezinfekci a sterilizaci všech zařízení v mlékárenských provozech. Proto je spolehlivá metoda monitorování přítomnosti bakteriofágů, ale zejména jejich kvantifikace, nutnou součástí boje proti virové infekci. Tato práce popisuje kvantifikaci laktokokového bakteriofága 936 pomocí polymerázové řetězové reakce (qPCR) a aplikaci tohoto postupu na vzorky provozních zákysů a syrovátek. Metoda je založena na amplifikaci a fluorescenční detekci nárůstu počtu kopií úseku genu pro hlavní strukturální protein fága 936 a je tudíž nezávislá na kultivaci bakteriofága. Metoda qPCR je rychlá, citlivá, umožňuje kvantifikaci bakteriofága 936 v malých objemech průmyslových vzorků a v kterékoliv fázi fermentačního procesu.\n
Keywords:
Bacteriophage 936; dairy products; lactobacillus
Available at various institutes of the ASCR
Bacteriophage 936 by qPCR quantification in dairy products.
Virulent bacteriophages are still representing significant threat in dairy production even with frequent disinfection and sterilization all the equipment in dairy plants. Therefore a reliable \nmethod ...
Sequencing of DNA variable fragment coding aspartyl-tRNA synthetase suitable for taxonomic classification and phylogenetic analysis of bacterial genera closely related to genus Lactobacillales \n
Killer, Jiří; Mrázek, Jakub
2018 - Czech
There was designed and tested a reliable marker for aspartyl-tRNA synthetase suitable for taxonomic classification and phylogenetic analysis of bacterial genera closely related to genus Lactobacillales. Method enables reliable a sensitive estimation in dairy starters and is independent on the phage cultivation. It is quick, reliable a sensitive estimation of dairy bacteria. It is possible to use it for basic research as well as for the effective analysis in dairy production plants. \n Byl připraven a ověřen marker (aspartyl-tRNA syntáza) pro klasifikaci a fylogenetické analýzy zástupců rodů náležejících do řádu Lactobacillales. Umožňuje rychlé a přesné druhové stanovení mléčných bakterií. Předkládanou metodiku lze využít v základním výzkumu při klasifikaci a fylogenetických analýzách vybraných rodů bakterií mléčného kvašení. Především je určen pro rychlé analýzy v provozech mléčného průmyslu. \n
Keywords:
Lactobacillales; PCR; marker
Available at various institutes of the ASCR
Sequencing of DNA variable fragment coding aspartyl-tRNA synthetase suitable for taxonomic classification and phylogenetic analysis of bacterial genera closely related to genus Lactobacillales \n
There was designed and tested a reliable marker for aspartyl-tRNA synthetase suitable for taxonomic classification and phylogenetic analysis of bacterial genera closely related to genus ...
In vivo IBD using minipig course results
Juhás, Štefan; Juhásová, Jana
2017 - Czech
Innovative approaches testing of Inflammatory Bowel Disease on minipigs. Testování inovativních postupů léčby IBD na miniaturních prasatech.
Keywords:
minipig; IBD; operational equipment
Available at various institutes of the ASCR
In vivo IBD using minipig course results
Innovative approaches testing of Inflammatory Bowel Disease on minipigs.
Results of the realisation of the experiments using the experimental animals
Juhás, Štefan; Juhásová, Jana
2017 - Czech
A design of methods to test a functionality of inovative replacements, solution of the replacement incl. implantation and connected agenda. Návrh způsobu ověření funkčnosti inovativních náhrad, řešení tohoto návrhu včetně implantací a souvisejících aktivit.
Keywords:
implants; experimental animals
Available at various institutes of the ASCR
Results of the realisation of the experiments using the experimental animals
A design of methods to test a functionality of inovative replacements, solution of the replacement incl. implantation and connected agenda.
Written report of the contractor on the results of the research in ophtalmology, implantation and cell biology.
Ardan, Taras
2017 - Czech
Research on the reaction of the organism and specific eye cells on the innovative implant developer by the contractor. Výzkum v oblasti reakce organismu a specifických buněk oka na inovativní implantát vyvinutý objednatelem.
Keywords:
intraocular; cells; implant
Available at various institutes of the ASCR
Written report of the contractor on the results of the research in ophtalmology, implantation and cell biology.
Research on the reaction of the organism and specific eye cells on the innovative implant developer by the contractor.
Implant study protocol including results
Juhás, Štefan; Juhásová, Jana
2017 - Czech
Testing and analysis of implant material and design in real environment. Ověření a analýza funkčnosti implantačního materiálu a designu v reálném prostředí.
Keywords:
implant; material; in vivo
Available at various institutes of the ASCR
Implant study protocol including results
Testing and analysis of implant material and design in real environment.
Results of in vivo nanomaterials testing using minipig
Juhás, Štefan; Juhásová, Jana
2017 - Czech
Using in vivo experiments we tested healing of a fresh wound treated with a nanofibrous cover. From predefined wounds, samples were taken at regular intervals for histological analysis, wound measurements and photodocumentation were performed. Pomocí in vivo experimentů jsme testovali hojení čerstvé rány ošetřené pomocí nanovlákenného krytu. Z předem definovaných ran se v pravidelných intervalech odebírali vzorky na histologickou analýzu, provádělo se měření ran a fotodokumentace.
Keywords:
minipig; skin wound; nanomaterial
Available at various institutes of the ASCR
Results of in vivo nanomaterials testing using minipig
Using in vivo experiments we tested healing of a fresh wound treated with a nanofibrous cover. From predefined wounds, samples were taken at regular intervals for histological analysis, wound ...
Results of nanomaterials testing for skin wounds nursing
Juhás, Štefan; Juhásová, Jana
2017 - Czech
Using minipigs experiments, we tested the healing of fresh wounds treated with different nanofibrous materials and compared them with standard treatment. From predefined wounds, samples were taken at regular intervals for histological analysis, microbiology, wound measurements and photodocumentation. Blood was also collected from the miniprasate to determine pro-inflammatory cytokines. Pomocí experimentů na miniprasatech jsme testovali hojení čerstvé rány ošetřené pomocí různých nanovlákenných materiálu a porovnávali se standardním ošetřením. Z předem definovaných ran se v pravidelných intervalech odebírali vzorky na histologickou analýzu, mikrobiologii, provádělo se měření ran a fotodokumentace. Z miniprasat se taky odebírala krev pro stanovení prozánětlivých cytokinů.
Keywords:
minipig; skin wound; nanomaterial; bandage
Available at various institutes of the ASCR
Results of nanomaterials testing for skin wounds nursing
Using minipigs experiments, we tested the healing of fresh wounds treated with different nanofibrous materials and compared them with standard treatment. From predefined wounds, samples were taken at ...
Mendel Forum 2014
Matalová, Eva; Matalová, A.
2014 - Czech
Keywords:
Mendel
Available at various institutes of the ASCR
Mendel Forum 2014
Methodology of „acute“and transgenic Huntington disease model design in miniature pigs and its application for new methods of treatment and drugs testing in neurodegenerative diseases area
Motlík, Jan; Juhás, Štefan; Juhásová, Jana; Vodička, Petr; Baxa, Monika
2014 - Czech
The aim of this methodology is to develop a biomedical model of Huntington's disease in miniature pigs and its use in practice for preclinical testing of new treatments both pharmacological and using new molecular biological methods. Cílem metodiky je vypracování biomedicínského modelu Huntingtonovy choroby u miniaturních prasat a jeho využití v preklinické praxi pro testování nových léčebných postupů jak farmakologických, tak pomocí nových molekulárně biologických metod.
Keywords:
Huntington´s disease
Available at various institutes of the ASCR
Methodology of „acute“and transgenic Huntington disease model design in miniature pigs and its application for new methods of treatment and drugs testing in neurodegenerative diseases area
The aim of this methodology is to develop a biomedical model of Huntington's disease in miniature pigs and its use in practice for preclinical testing of new treatments both pharmacological and using ...
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