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Sample preparation for single cell analysis
Adamová, Eva; Basova, E. Y.; Potáčová, Anna; Foret, František; Matalová, Eva; Klepárník, Karel
2014 - anglický
Development of sensitive, miniaturized and fast methods aims to reliable analyze even small amounts of samples. Micromanipulation systems such as laser capture microdissection enable to collect just single cells from cell suspensions or tissue samples. One option for further analysis is the bioluminescence reaction based on luciferin/luciferase conversion. This approach was used for detection of active caspases, caspase-3/7. Droplets based microfluidic systems provide benefits in biological, pharmaceutical and chemical research.
Klíčová slova:
single cell analysis; micromanipulation; bioluminiscence
Dokument je dostupný na externích webových stránkách.
Sample preparation for single cell analysis
Development of sensitive, miniaturized and fast methods aims to reliable analyze even small amounts of samples. Micromanipulation systems such as laser capture microdissection enable to collect just ...
Theoretical principles of capillary isotachophoresis in moving boundary systems
Gebauer, Petr; Malá, Zdeňka; Boček, Petr
2014 - anglický
This contribution provides fundamental theoretical background of ITP in moving-boundary systems. It is based on the approximation of capillary ITP as a 1D process and the general condition of ITP migration, i.e., the existence of a self-sharpening boundary capable to form a stack of zones moving with equal velocities.
Klíčová slova:
capillary isotachophoresis; moving boundary system; stacking capability
Dokument je dostupný na externích webových stránkách.
Theoretical principles of capillary isotachophoresis in moving boundary systems
This contribution provides fundamental theoretical background of ITP in moving-boundary systems. It is based on the approximation of capillary ITP as a 1D process and the general condition of ITP ...
Separation of tryptic digest of cytochrome c within a long nanoelectrospray tip
Týčová, Anna; Foret, František
2014 - anglický
Capillary electrophoresis coupled to mass spectrometry (CE-MS) posses powerful tool for separation and detection of wide range of ionic species. To avoid complicated constructions of interfaces we have conducted the separation within a long and thin nanoelectrospray tip [1]. We have investigated main properties of such an experimental design on a complex sample of tryptic digest of cytochrome c.
Klíčová slova:
capillary electrophoresis; cytochrome c; nanoelectrospray
Dokument je dostupný na externích webových stránkách.
Separation of tryptic digest of cytochrome c within a long nanoelectrospray tip
Capillary electrophoresis coupled to mass spectrometry (CE-MS) posses powerful tool for separation and detection of wide range of ionic species. To avoid complicated constructions of interfaces we ...
Arsenic speciation analysis by HPLC postcolumn hydride generation and detection by atomic fluorescence spectrometry
Marschner, Karel; Musil, Stanislav; Rychlovský, P.; Dědina, Jiří
2014 - anglický
The aim of this contribution is to present a new method of hydride generation that enables to generate arsines from iAs , iAs , MMA and DMA in a flow injection mode with the same efficiency and in the next step connection of this hydride generator with HPLC column.
Klíčová slova:
arsenic speciation analysis; hydride generation; atomic fluorescence spectrometry
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Arsenic speciation analysis by HPLC postcolumn hydride generation and detection by atomic fluorescence spectrometry
The aim of this contribution is to present a new method of hydride generation that enables to generate arsines from iAs , iAs , MMA and DMA in a flow injection mode with the same efficiency and in the ...
Analysis of individual organelles
Václavek, Tomáš; Manion-Fisher, D.; Muratore, K.; Taylor, T.; Křenková, Jana; Foret, František; Arriaga, E. A.
2014 - anglický
This poster describes a method of organelle analysis using capillary electrophoresis instrumentation with high sensitivity laser induced fluorescence detection.
Klíčová slova:
organelle; capillary; fluorescence
Dokument je dostupný na externích webových stránkách.
Analysis of individual organelles
This poster describes a method of organelle analysis using capillary electrophoresis instrumentation with high sensitivity laser induced fluorescence detection.
Identification of nosocomial infection by electrophoretic techniques
Kubesová, Anna; Moravcová, Dana; Tesařová, Marie; Horká, Marie
2014 - anglický
The detection and identification of pathogens currently relies upon a very diverse range of techniques and skills, from traditional culturing and taxonomic procedures to modern molecular biology based methods. However, conventional laboratory methods are time consuming, laborious, and they may provide both false positive or negative results, especially for closely related microorganisms. In this study, we suggest capillary electrophoresis techniques for differentiation and characterization of Methicillin-resistant and Methicillin-susceptible Staphylococcus aureus.
Klíčová slova:
capillary electrophoresis; nosocomial infection; Staphylococcus aureus
Dokument je dostupný na externích webových stránkách.
Identification of nosocomial infection by electrophoretic techniques
The detection and identification of pathogens currently relies upon a very diverse range of techniques and skills, from traditional culturing and taxonomic procedures to modern molecular biology based ...
Computer assisted development of a pneumatic electrospray nebulizer
Járvás, G.; Szigeti, M.; Grym, Jakub; Foret, František; Guttman, A.
2014 - anglický
In this study we demonstrate how computer simulations can assist the development of a novel microfabricated pneumatic electrospray nebulizer. The microdevice under consideration is designed for electrospray mass spectrometry interfacing without the need to fabricate an electrospray needle. This new interfacing strategy requires comprehensive evaluation of the differently designed chip layouts. The flow evaluation was supported by computational fluid dynamics simulations. It was proved with experimental validation of the simulation results that the developed models were practical tools for the numerical investigation of the separations-ESI-MS coupling.
Klíčová slova:
microfabricated ESI-MS; electrospray mass spectrometry; pneumatic electrospray nebulizer
Dokument je dostupný na externích webových stránkách.
Computer assisted development of a pneumatic electrospray nebulizer
In this study we demonstrate how computer simulations can assist the development of a novel microfabricated pneumatic electrospray nebulizer. The microdevice under consideration is designed for ...
Isotachophoretic determination of ketone bodies and lactate in cattle serum
Vyskočilová, V.; Pechová, A.; Pantůčková, Pavla
2014 - anglický
The aim of the work was the optimization of isotachophoretic determination of lactate and ketone bodies (beta-hydroxybutyrate and acetoacetate) for veterinary medicine. The measurement of these metabolites is very important for the evaluation of metabolic diseases of cattle. The complete separation of these three analytes together with other possible blood components was achieved using 10 mmol/l hydrochloric acid with beta-alanine as a leading electrolyte at pH = 3.15 and 10 mmol/l sodium propionate as a terminating electrolyte. Limits of detection determined from the standard mixture for acetoacetate, lactate and beta-hydroxybutyrate were 3.75 mu mol/l, 3.96 mu mol/l and 7.46 mu mol/l, respectively.
Klíčová slova:
cattle serum; lactate; ketone bodies; isotachophoresis
Dokument je dostupný na externích webových stránkách.
Isotachophoretic determination of ketone bodies and lactate in cattle serum
The aim of the work was the optimization of isotachophoretic determination of lactate and ketone bodies (beta-hydroxybutyrate and acetoacetate) for veterinary medicine. The measurement of these ...
Microbicidal properties and chemical composition of essential oils
Křůmal, Kamil; Večeřa, Zbyněk
2014 - anglický
The microbicidal properties of 6 essential oils (EOs; Lavandula angustifolia, Cymbopogon nardus, Citrus aurantifolia, Juniperus communis, Myrtus communis and Cinnamomum zeylanicum ) for 17 microorganisms were determined using the vapour-agar contact method. The most effective EO (i.e. Lavandula angustifolia ) whose volatile components provided the sufficient microbicidal properties was chosen for detailed study of chemical composition.
Klíčová slova:
essential oil; microbicidal properties; denuder
Dokument je dostupný na externích webových stránkách.
Microbicidal properties and chemical composition of essential oils
The microbicidal properties of 6 essential oils (EOs; Lavandula angustifolia, Cymbopogon nardus, Citrus aurantifolia, Juniperus communis, Myrtus communis and Cinnamomum zeylanicum ) for 17 ...
Direct coupling of supported liquid membrane extractions to capillary electrophoresis. Sensitivity enhancement in analyses of untreated complex samples
Pantůčková, Pavla; Kubáň, Pavel; Boček, Petr
2014 - anglický
Extractions across supported liquid membrane (SLM) were in-line coupled to capillary electrophoresis (CE) commercial system for direct injection of basic drugs from raw complex samples. A micro-extraction unit was inserted into the CE system sampling device and ensured excellent sample clean-up and injections directly from the membrane surface. Transient isotachophoresis (t-ITP) combined with large electrokinetic injections of selectively extracted basic analytes increased the sensitivity of the analytical method up to 300-fold when compared with conventional hydrodynamic injections and capillary zone electrophoresis (CZE) of the basic analytes.
Klíčová slova:
capillary electrophoresis; supported liquid membrane; transient isotachophoresis
Dokument je dostupný na externích webových stránkách.
Direct coupling of supported liquid membrane extractions to capillary electrophoresis. Sensitivity enhancement in analyses of untreated complex samples
Extractions across supported liquid membrane (SLM) were in-line coupled to capillary electrophoresis (CE) commercial system for direct injection of basic drugs from raw complex samples. A ...
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