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Determination of biological active compounds in pressurized water extract of Sambucus nigra L. branches
Hohnová, Barbora; Šalplachta, Jiří
2015 - anglický
GC-MS and SDS-PAGE methods were employed for determination of carbohydrates and biological active proteins in pressurized hot water extract of Sambucus nigra L. branches.
Klíčová slova:
PHWE; GC-MS; SDS-PAGE
Dokument je dostupný na externích webových stránkách.
Determination of biological active compounds in pressurized water extract of Sambucus nigra L. branches
GC-MS and SDS-PAGE methods were employed for determination of carbohydrates and biological active proteins in pressurized hot water extract of Sambucus nigra L. branches.
Simple route of caspase-3 FRET sensor synthesis using “click chemistry”
Lišková, Marcela; Křenková, Jana; Klepárník, Karel; Pazdera, P.; Foret, František
2015 - anglický
Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences Asp-Glu-Val-Asp (DEVD) on their substrates and hydrolyze peptide bonds after aspartic acid residues. Various techniques for the determination of caspase-3 are commercially available e.g. Enzyme Linked Immuno-Sorbent Assay (ELISA), Western blotting or flow cytometric analysis. The products of the cleavage can be detected by spectrophotometry, fluorimetry, chemiluminescence (CL) or ELISA. In this work, we suggested fluorescent sensor based on easily prepared Förster Resonance Energy Transfer (FRET). We use very simple chemistry called “click”. This type of chemistry takes advantages of quickness, simplicity and cheapness. “Click chemistry” is based on usage of various functional group and cross-linkers to combine individual molecules together.
Klíčová slova:
caspases; click chemistry; cell analysis
Dokument je dostupný na externích webových stránkách.
Simple route of caspase-3 FRET sensor synthesis using “click chemistry”
Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences ...
Low-cost 3D printed device for fabrication of nanoESI tips by grinding
Přikryl, Jan; Týčová, Anna; Foret, František
2015 - anglický
The aim of this work was the development of a simple and low-cost device for nanospray tips fabrication. Although there are several ways of the tip preparation, grinding was chosen as the most suitable in this work. Inexpensive, flexible and reliable method of 3D printing was used for the device construction making it available for any research group with a 3D printer.
Klíčová slova:
3D printing; grinding; nanospray tip
Dokument je dostupný na externích webových stránkách.
Low-cost 3D printed device for fabrication of nanoESI tips by grinding
The aim of this work was the development of a simple and low-cost device for nanospray tips fabrication. Although there are several ways of the tip preparation, grinding was chosen as the most ...
Quantum dot-based immunoprobe for optical and electrochemical detection
Dvořáková, V.; Čadková, M.; Datinská, Vladimíra; Chałupniak, A.; Korecká, L.; Merkoci, A.; Klepárník, Karel; Foret, František; Bílková, Z.
2015 - anglický
Three-stage labeling strategy allows the preparation of specific conjugates composed of required antibodies and quantum dots as an extremely sensitive bio-probe applicable in varied immunoassays with following optical and electrochemical detection. This approach is essentially based on carbodiimide chemistry however each single step of whole procedure is separated and controlled which makes this applied protocol oriented, highly efficient and it enables gain of pure antibody-conjugated quantum dots in ready-to-use condition.
Klíčová slova:
quantum dots; conjugation; carbodiimide coupling; IgG molecules
Dokument je dostupný na externích webových stránkách.
Quantum dot-based immunoprobe for optical and electrochemical detection
Three-stage labeling strategy allows the preparation of specific conjugates composed of required antibodies and quantum dots as an extremely sensitive bio-probe applicable in varied immunoassays with ...
Study of the cell wall of Staphylococcus aureus and its sensitivity to enzybiotics
Čmelík, Richard; Melková, K.; Kobzová, Š.; Janda, L.
2015 - anglický
The endolysin resistant and sensitive strains of Staphylococcus aureus were compared by means of LC-MS based structural analysis of peptidoglycan isolated from their cell walls. The structural explanation of the resistance was suggested.
Klíčová slova:
peptidoglycan; LC-MS; enzybiotics
Dokument je dostupný na externích webových stránkách.
Study of the cell wall of Staphylococcus aureus and its sensitivity to enzybiotics
The endolysin resistant and sensitive strains of Staphylococcus aureus were compared by means of LC-MS based structural analysis of peptidoglycan isolated from their cell walls. The structural ...
Simple flow-focusing microfluidic chip for droplet generation
Křivánková, Jana; Basova, E. Y.; Foret, František
2015 - anglický
There is an ascending requirement for analysis of chemical content of individual cells involving combination of various bioanalytical and optical sensors and arrangements. At the beginning of the cell analysis there is a need for creation of suitable transport vessels - droplets - that can be easily counted, sorted and serve for manipulation of cells with preserved biocompability as well. Among all of bioanalytical approaches droplet-based microfluidics proved its potential for producing droplets with precisely controlled size, shape as well as for encapsulation of reactants and delivering drugs. This study presents a simple microfluidic chip with flow-focusing geometry for generation of water droplets in fluorocarbon oil.
Klíčová slova:
microfluidic chip; droplet generation; fluorinated oil phase
Dokument je dostupný na externích webových stránkách.
Simple flow-focusing microfluidic chip for droplet generation
There is an ascending requirement for analysis of chemical content of individual cells involving combination of various bioanalytical and optical sensors and arrangements. At the beginning of the cell ...
Efficient generation of arsanes coupled with HPLC or with cryotrapping for speciation analysis of arsenic
Marschner, Karel; Musil, Stanislav; Rychlovský, P.; Dědina, Jiří
2015 - anglický
Arsenic speciation analysis by HPLC postcolumn hydride generation or with cryotrapping and detection by atomic fluorescence spectrometry.
Klíčová slova:
arsenic speciation analysis; hydride generation; HPLC
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Efficient generation of arsanes coupled with HPLC or with cryotrapping for speciation analysis of arsenic
Arsenic speciation analysis by HPLC postcolumn hydride generation or with cryotrapping and detection by atomic fluorescence spectrometry.
Comparison of ionisation properties of aetma-labeled saccharides with common labels
Partyka, Jan; Foret, František
2015 - anglický
We have tested (2-aminoethyl)trimethylammonium (AETMA) as a label for analysis of oligosaccharides by capillary electrophoresis with electrospray (ESI) mass spectrometry detection and compared its performance to taurine, 2-aminobenzoic acid, 2-aminobenzamide and 8-aminopyrene-1,3,6-trisulfonic acid. The AETMA-labeled saccharides were provided higher ionization signals in positive mode than native sodium or ammonium adducts and equal or higher signals than saccharides labeled by the more commonly used labels tested in this study.
Klíčová slova:
saccharides; AETMA; ESI/MS
Dokument je dostupný na externích webových stránkách.
Comparison of ionisation properties of aetma-labeled saccharides with common labels
We have tested (2-aminoethyl)trimethylammonium (AETMA) as a label for analysis of oligosaccharides by capillary electrophoresis with electrospray (ESI) mass spectrometry detection and compared its ...
System for fast analysis of explosives in the enviromnemt
Čapka, Lukáš; Večeřa, Zbyněk; Mikuška, Pavel; Šesták, Jozef; Kahle, Vladislav
2015 - anglický
Detection of explosives is the subject of many investigations by law enforcement agents and forensic scientist. The identification and discrimination of evidence taken from crime scenes is a common practice in forensic investigations. The higher demands on simple and fast determination of hazard materials or strange objects are required in connection with worldwide often repeated terrorist activities.
Klíčová slova:
explosives; hazard materials; energetic materials; portable device for the analysis
Dokument je dostupný na externích webových stránkách.
System for fast analysis of explosives in the enviromnemt
Detection of explosives is the subject of many investigations by law enforcement agents and forensic scientist. The identification and discrimination of evidence taken from crime scenes is a common ...
Thiol-ene-based monolithic enzymatic microreactor for glycoprotein deglycosylation
Novotný, Jakub; Lafleur, J. P.; Kutter, J. P.; Foret, František
2015 - anglický
Enzymatic microreactor was fabricated from thiol-ene polymer. Glycoprotein samples were deglycosylated by enzyme PNGase F which was immobilized on the monolith.
Klíčová slova:
thiol-enes; mictoreactor; monolith
Dokument je dostupný na externích webových stránkách.
Thiol-ene-based monolithic enzymatic microreactor for glycoprotein deglycosylation
Enzymatic microreactor was fabricated from thiol-ene polymer. Glycoprotein samples were deglycosylated by enzyme PNGase F which was immobilized on the monolith.
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