Carotenoid pool of extremophilic bacterium Deinococcus radiodurans
POKORNÁ, Natálie
2021 - English The thesis focuses on the carotenoid composition of the extremophilic bacterium D. radiodurans. The practical part of the thesis was focused on the composition of the carotenoid pool and the identification of the respective peaks of the chromatogram. Lastly, the influence of the age of the culture and exposure of the UV light was studied and evaluated. The thesis focuses on the carotenoid composition of the extremophilic bacterium D. radiodurans. The practical part of the thesis was focused on the composition of the carotenoid pool and the identification of the respective peaks of the chromatogram. Lastly, the influence of the age of the culture and exposure of the UV light was studied and evaluated. Keywords: Deinococcus radiodurans; bacteria; carotenoids; HPLC Available in the Digital Repository of University of South Bohemia. Carotenoid pool of extremophilic bacterium Deinococcus radiodurans

The thesis focuses on the carotenoid composition of the extremophilic bacterium D. radiodurans. The practical part of the thesis was focused on the composition of the carotenoid pool and the ...

POKORNÁ, Natálie
Jihočeská univerzita v Českých Budějovicích, 2021

Production and Biophysical Characterization of Phi8P4 Helicase
MOURA, Siroun
2020 - English The goal of this thesis was cloning of Phi8P4 protein into expression vector with N-terminal his-tag, optimization of protein expression and large-scale production for further functional and crystal structure studies with RNA. The goal of this thesis was cloning of Phi8P4 protein into expression vector with N-terminal his-tag, optimization of protein expression and large-scale production for further functional and crystal structure studies with RNA. Keywords: dsRNA viruses; bacteriophages; P4 protein; Phi8P4; N-terminal His-tag Available in the Digital Repository of University of South Bohemia. Production and Biophysical Characterization of Phi8P4 Helicase

The goal of this thesis was cloning of Phi8P4 protein into expression vector with N-terminal his-tag, optimization of protein expression and large-scale production for further functional and crystal ...

MOURA, Siroun
Jihočeská univerzita v Českých Budějovicích, 2020

Pojetí věrnosti v Maloryho zpracování artušovských legend
HŘEBEJKOVÁ, Aneta
2020 - English This bachelor thesis deals with the conception of loyalty in Sir Thomas Malory's Le Morte d'Arthur. The objective of this work is to define the abstract term of loyalty and analyse two stories from Le Morte d'Arthur, in which loyalty plays a key role. This thesis also briefly introduces chivalry and personage of legendary king Arthur, due to better understanding and orientation in Malory's book. Předkládaná bakalářská práce se zabývá pojetím věrnosti v díle sira Thomase Malory, Le Morte d'Arthur (Artušova smrt). Záměrem práce je představit nesnadno definovatelný pojem věrnosti a posléze jeho prostřednictvím zanalyzovat dvojici příběhů z Le Morte d'Arthur, v nichž zastupuje věrnost klíčovou roli. Pro lepší pochopení a orientaci v Maloryho díle práce přibližuje ještě rytířskou kulturu a samostatnou postavu legendárního krále Artuše. Keywords: Thomas Malory; Artušova smrt; věrnost; rytířství; víra Available in the Digital Repository of University of South Bohemia. Pojetí věrnosti v Maloryho zpracování artušovských legend

This bachelor thesis deals with the conception of loyalty in Sir Thomas Malory's Le Morte d'Arthur. The objective of this work is to define the abstract term of loyalty and analyse two stories from Le ...

HŘEBEJKOVÁ, Aneta
Jihočeská univerzita v Českých Budějovicích, 2020

Understanding motivation of Czech high school and university students to learn the English and French languages
CSEVÁROVÁ, Marie
2020 - English The focus of this bachelor thesis is the study of the motivation of two target groups: high school and university students in learning both English and French languages. The research was stimulated by the great influence of motivation on Second Language Acquisition (SLA), and it aimed to give an insight into the motivations and different variables that influence the motivation of the students. The first chapter of the thesis introduces the topic of motivation in SLA, serving as the basis for the research. Accordingly, this work focused specifically on the following aspects of motivation: intrinsic and extrinsic motivation, instrumental motivation, language anxiety and amotivation, and several of the influences on the motivation of the students: teachers, parents, and friends. It is followed by the second chapter, which outlines the methodology: piloting a questionnaire, final questionnaire design, and the data collection. The third chapter contains the data analysis, which comprises the responses of the participants. The fourth chapter addresses both contrasts and similarities between the two studied groups. Several of the main findings are - Within the students, intrinsic and extrinsic motives coexist, nevertheless, for university students, the career motive was more noticeable. Concerning high school students, intrinsic motivation was the most prevalent. There were also some differences between the students of the target languages of this thesis, French students were often motivated by the culture of the language, and English students mostly perceived the practical usefulness of English as their main motive. The influence of teachers was perceived as more positive within university students than the high school students. Both groups of students expressed quite low perceptions of the active parental influence on their language learning. Many students displayed the occasional state of amotivation or signs of language anxiety. These aspects were higher within the university students, French majors in particular. This thesis could serve as a base for follow up research or as a tool for educators in researching the motives of their students in order to increase their motivation. Tato bakalářská práce je zaměřena na motivaci dvou cílových skupin: studentů středních a vysokých škol při výuce anglického a francouzského jazyka. Inspirace pro tuto práci byl potencionální vliv motivace na učení se druhého jazyka (SLA) a cílem bylo porozumět motivaci a různých proměnných, které ovlivňují motivaci studentů. První kapitola práce představuje téma motivace v SLA, které slouží jako základ pro výzkum. Tato práce se tedy zaměřila konkrétně na tyto aspekty motivace: vnitřní a vnější motivace, instrumentální motivace, jazyková úzkost a amotivace a několik vlivů na motivaci studentů: učitelé, rodiče a přátelé. Následuje druhá kapitola, která nastiňuje metodologii výzkumu: pilotní dotazník, návrh finálního dotazníku a sběr dat. Třetí kapitola obsahuje analýzu dat, která zahrnuje odpovědi účastníků. Čtvrtá kapitola pojednává o kontrastech a podobnostech mezi oběma studovanými skupinami. Několik hlavních zjištění: Mezi studenty existují v symbióze vnitřní a vnější motivy, u vysokoškolských studentů byl však zřetelnější kariérní motiv. Pokud jde o studenty středních škol, nejrozšířenější byla vnitřní motivace, což znamená že se učí jazyk, protože je to baví. Určité rozdíly se našly i mezi studenty cílových jazyků této práce, studenti francouzštiny byli často motivováni kulturou jazyka a studenti angličtiny většinou vnímali praktickou užitečnost angličtiny jako svůj hlavní motiv. Vliv učitelů byl vnímán jako pozitivnější u studentů vysokých škol více než u studentů středních škol. Obě skupiny studentů projevily poměrně nízké vnímání aktivního rodičovského vlivu na jejich jazykové vzdělávání. Mnoho studentů projevilo občasný stav amotivace nebo známky jazykové úzkosti. Tyto aspekty byly vyšší u studentů vysokých škol, a to zejména studentů francouzštiny. Tato práce by mohla sloužit jako základ pro následující výzkum nebo jako nástroj pro pedagogy při zkoumání motivů jejich studentů za účelem zvýšení jejich motivace. Keywords: motivace; SLA; francouzský jazyk; anglický jazyk; vnitřní motivy; vnější motivy; amotivace Available in the Digital Repository of University of South Bohemia. Understanding motivation of Czech high school and university students to learn the English and French languages

The focus of this bachelor thesis is the study of the motivation of two target groups: high school and university students in learning both English and French languages. The research was stimulated by ...

CSEVÁROVÁ, Marie
Jihočeská univerzita v Českých Budějovicích, 2020

Understanding the interactions between Borrelia duttonii and the tick Ornithodoros moubata as well as the mammalian host at the in vitro and in vivo level
BAYER, Maximilian
2020 - English The aim of this Bachelor thesis was to study the interactions between the spirochete B. duttonii, which is a causative agent for relapsing fever in Africa, and its vector, the soft tick O. moubata. Several experiments were conducted including passaging, membrane feeding and keeping infected ticks at different temperatures. Microscopy experiments were carried out to visualize the presence of B. duttonii in mammalian blood cells and in the salivary glands of the O. moubata tick. The aim of this Bachelor thesis was to study the interactions between the spirochete B. duttonii, which is a causative agent for relapsing fever in Africa, and its vector, the soft tick O. moubata. Several experiments were conducted including passaging, membrane feeding and keeping infected ticks at different temperatures. Microscopy experiments were carried out to visualize the presence of B. duttonii in mammalian blood cells and in the salivary glands of the O. moubata tick. Keywords: Molecular ecology; vectors and parasites; relapsing fever; Borrelia; soft tick Available in the Digital Repository of University of South Bohemia. Understanding the interactions between Borrelia duttonii and the tick Ornithodoros moubata as well as the mammalian host at the in vitro and in vivo level

The aim of this Bachelor thesis was to study the interactions between the spirochete B. duttonii, which is a causative agent for relapsing fever in Africa, and its vector, the soft tick O. moubata. ...

BAYER, Maximilian
Jihočeská univerzita v Českých Budějovicích, 2020

Transformation of marine protist Diplonema papillatum - characterization of selected cell lines
GRAF, Lena
2020 - English The aim of this thesis was to analyze and characterize selected transformant cell lines of marine protist Diplonema papillatum. The aim of this thesis was to analyze and characterize selected transformant cell lines of marine protist Diplonema papillatum. Keywords: Diplonema papillatum; marine protist; gene tagging; gene replacement Available in the Digital Repository of University of South Bohemia. Transformation of marine protist Diplonema papillatum - characterization of selected cell lines

The aim of this thesis was to analyze and characterize selected transformant cell lines of marine protist Diplonema papillatum.

GRAF, Lena
Jihočeská univerzita v Českých Budějovicích, 2020

The novel kinetoplastid kinesin TbKIFx and its partner TbPH1 are associated with specific cytoskeletal structures of Trypanosoma brucei
MÜLLER, Nora
2019 - English This study focuses on the kinesin motor protein TbKIFx and its presumably catalytically inactive partner TbPH1 in Trypanosoma brucei. Not much is known about the TbPH1/TbKIFx dimer so far. The aim of our research was to localize TbKIFx/TbPH1 in procyclic and bloodstream cells and to investigate potential cargo candidates. We believe that we identified the microtubule quartet and mature basal body as potential binding sites of TbKIFx/TbPH1 in both life cycle stages of T. brucei. However, our investigations into tyrosine-phosphorylated proteins as possible cargo of TbKIFx/TbPH1 remain inconclusive. Available in the Digital Repository of University of South Bohemia. The novel kinetoplastid kinesin TbKIFx and its partner TbPH1 are associated with specific cytoskeletal structures of Trypanosoma brucei

This study focuses on the kinesin motor protein TbKIFx and its presumably catalytically inactive partner TbPH1 in Trypanosoma brucei. Not much is known about the TbPH1/TbKIFx dimer so far. The aim of ...

MÜLLER, Nora
Jihočeská univerzita v Českých Budějovicích, 2019

Novel non-coding transcripts at imprinted loci in mammalian oocytes and embryos
NENIN, Nina
2019 - English Aims of this thesis were to annotate novel transcripts within clusters of imprinted genes in mouse oocytes and embryo, to analyze expression changes of these transcripts during mouse embryonic development, to identify enriched sequence motifs and potential transcription factors binding sites at promoters of transcripts within imprinted gene clusters as well as transposable elements acting as promoters of these transcripts and to identify potential candidates for further functional studies, using bioinformatic methods. Aims of this thesis were to annotate novel transcripts within clusters of imprinted genes in mouse oocytes and embryo, to analyze expression changes of these transcripts during mouse embryonic development, to identify enriched sequence motifs and potential transcription factors binding sites at promoters of transcripts within imprinted gene clusters as well as transposable elements acting as promoters of these transcripts and to identify potential candidates for further functional studies, using bioinformatic methods. Keywords: genetics; bioinformatics; imprinting in mammals; novel trananscripts; expression analysis; imprinted genes; mouse transcriptome; transcription factors; regulation of imprinting; transposable elements Available in the Digital Repository of University of South Bohemia. Novel non-coding transcripts at imprinted loci in mammalian oocytes and embryos

Aims of this thesis were to annotate novel transcripts within clusters of imprinted genes in mouse oocytes and embryo, to analyze expression changes of these transcripts during mouse embryonic ...

NENIN, Nina
Jihočeská univerzita v Českých Budějovicích, 2019

Changes of IgM expression levels in the head kidney of Cyprinus carpio following an infection with Sphareospora molnari
SIERANSKI, Viktor
2019 - English Sphaerospora molnar is a myxozoan inducing sphaerosporosis in common carp and causing serious damage to secondary lamellae and epithelia of the gills. The understanding of the host immune response against S. molnari as well as other myxozoans is very limited in part due to the unresolved life cycle. The focus of this thesis was the quantification of membrane bound and secretory IgM expression levels in the head kidney over a period of 9 weeks following an intraperitoneal injection of S. molnari blood stages. Significant increases in the expression levels of both IgMs were observed reaching its maximum at 42 days after infection. Two weeks following the infection the first significant increases of secretory IgM were detected. Keywords: IgM expression; head kidney; Sphaerospora molnari; Myxozoa; cnidaria; Cyprinus carpio Available in the Digital Repository of University of South Bohemia. Changes of IgM expression levels in the head kidney of Cyprinus carpio following an infection with Sphareospora molnari

Sphaerospora molnar is a myxozoan inducing sphaerosporosis in common carp and causing serious damage to secondary lamellae and epithelia of the gills. The understanding of the host immune response ...

SIERANSKI, Viktor
Jihočeská univerzita v Českých Budějovicích, 2019

Generating and classifying molecules
MOSER, Stefan
2019 - English Available in the Digital Repository of University of South Bohemia. Generating and classifying molecules

MOSER, Stefan
Jihočeská univerzita v Českých Budějovicích, 2019